Exoskeletal predator defenses of juvenile California spiny lobsters (Panulirus interruptus) are affected by fluctuating ocean acidification-like conditions

Spiny lobsters rely on multiple biomineralized exoskeletal predator defenses that may be sensitive to ocean acidification (OA). Compromised mechanical integrity of these defensive structures may tilt predator-prey outcomes, leading to increased mortality in the lobsters’ environment. Here, we tested the effects of OA-like conditions on the mechanical integrity of selected exoskeletal defenses of juvenile California spiny lobster, Panulirus interruptus. Young spiny lobsters reside in kelp forests with dynamic carbonate chemistry due to local metabolism and photosynthesis as well as seasonal upwelling, yielding daily and seasonal fluctuations in pH. Lobsters were exposed to a series of stable and diurnally fluctuating reduced pH conditions for three months (ambient pH/stable, 7.97; reduced pH/stable 7.67; reduced pH with low fluctuations, 7.67 ± 0.05; reduced pH with high fluctuations, 7.67 ± 0.10), after which we examined the intermolt composition (Ca and Mg content), ultrastructure (cuticle and layer thickness), and mechanical properties (hardness and stiffness) of selected exoskeletal predator defenses. Cuticle ultrastructure was consistently robust to pH conditions, while mineralization and mechanical properties were variable. Notably, the carapace was less mineralized under both reduced pH treatments with fluctuations, but with no effect on material properties, and the rostral horn had lower hardness in reduced/high fluctuating conditions without a corresponding difference in mineralization. Antennal flexural stiffness was lower in reduced, stable pH conditions compared to the reduced pH treatment with high fluctuations and not correlated with changes in cuticle structure or mineralization. These results demonstrate a complex relationship between mineralization and mechanical properties of the exoskeleton under changing ocean chemistry, and that fluctuating reduced pH conditions can induce responses not observed under the stable reduced pH conditions often used in OA research. Furthermore, this study shows that some juvenile California spiny lobster exoskeletal defenses are responsive to changes in ocean carbonate chemistry, even during the intermolt period, in ways that can potentially increase susceptibility to predation among this critical life stage

Square Root Compression and Noise Effects in Digitally Transformed Images

We report on a particular example of noise and data representation interacting to introduce systematic error into scientific measurements. Many instruments collect integer digitized values and apply nonlinear coding, in particular square root coding, to compress the data for transfer or downlink; this can introduce surprising systematic errors when they are decoded for analysis. Square root coding and subsequent decoding typically introduces a variable &plusmn;1 count value-dependent systematic bias in the data after reconstitution. This is significant when large numbers of measurements (e.g., image pixels) are averaged together. Using direct modeling of the probability distribution of particular coded values in the presence of instrument noise, one may apply Bayes' theorem to construct a decoding table that reduces this error source to a very small fraction of a digitizer step; in our example, systematic error from square root coding is reduced by a factor of 20 from 0.23 to 0.012 count rms. The method is suitable both for new experiments such as the upcoming PUNCH mission, and also for post facto application to existing data sets&mdash;even if the instrument noise properties are only loosely known. Further, the method does not depend on the specifics of the coding formula, and may be applied to other forms of nonlinear coding or representation of data values. &nbsp;</p

Optical Properties of Deep Ice at the South Pole - Absorption

We discuss recent measurements of the wavelength-dependent absorption coefficients in deep South Pole ice. The method uses transit time distributions of pulses from a variable-frequency laser sent between emitters and receivers embedded in the ice. At depths of 800 to 1000 m scattering is dominated by residual air bubbles, whereas absorption occurs both in ice itself and in insoluble impurities. The absorption coefficient increases approximately exponentially with wavelength in the measured interval 410 to 610 nm. At the shortest wavelength our value is about a factor 20 below previous values obtained for laboratory ice and lake ice; with increasing wavelength the discrepancy with previous measurements decreases. At around 415 to 500 nm the experimental uncertainties are small enough for us to resolve an extrinsic contribution to absorption in ice: submicron dust particles contribute by an amount that increases with depth and corresponds well with the expected increase seen near the Last Glacial Maximum in Vostok and Dome C ice cores. The laser pulse method allows remote mapping of gross structure in dust concentration as a function of depth in glacial ice.Comment: 26 pages, LaTex, Accepted for publication in Applied Optics. 9 figures, not included, available on request from [email protected]

Physics and Astrophysics of Strange Quark Matter

3-flavor quark matter (strange quark matter; SQM) can be stable or metastable for a wide range of strong interaction parameters. If so, SQM can play an important role in cosmology, neutron stars, cosmic ray physics, and relativistic heavy-ion collisions. As an example of the intimate connections between astrophysics and heavy-ion collision physics, this Chapter gives an overview of the physical properties of SQM in bulk and of small-baryon number strangelets; discusses the possible formation, destruction, and implications of lumps of SQM (quark nuggets) in the early Universe; and describes the structure and signature of strange stars, as well as formation and detection of strangelets in cosmic rays. It is concluded, that astrophysical and laboratory searches are complementary in many respects, and that both should be pursued to test the intriguing possibility of a strange ground state for hadronic matter, and (more generally) to improve our knowledge of the strong interactions.Comment: 45 pages incl. figures. To appear in "Hadrons in Dense Matter and Hadrosynthesis", Lecture Notes in Physics, Springer Verlag (ed. J.Cleymans

The AMANDA Neutrino Telescope: Principle of Operation and First Results

AMANDA is a high-energy neutrino telescope presently under construction at the geographical South Pole. In the Antarctic summer 1995/96, an array of 80 optical modules (OMs) arranged on 4 strings (AMANDA-B4) was deployed at depths between 1.5 and 2 km. In this paper we describe the design and performance of the AMANDA-B4 prototype, based on data collected between February and November 1996. Monte Carlo simulations of the detector response to down-going atmospheric muon tracks show that the global behavior of the detector is understood. We describe the data analysis method and present first results on atmospheric muon reconstruction and separation of neutrino candidates. The AMANDA array was upgraded with 216 OMs on 6 new strings in 1996/97 (AMANDA-B10), and 122 additional OMs on 3 strings in 1997/98.Comment: 36 pages, 23 figures, submitted to Astroparticle Physic

Measurements of the Cosmic-Ray Positron Fraction From 1 to 50 GeV

Two measurements of the cosmic-ray positron fraction as a function of energy have been made using the High Energy Antimatter Telescope (HEAT) balloon-borne instrument. The first flight took place from Ft. Sumner, New Mexico in 1994, and yielded results above the geomagnetic cutoff energy of 4.5 GeV. The second flight from Lynn Lake, Manitoba in 1995 permitted measurements over a larger energy interval, from 1 GeV to 50 GeV. In this letter we present results on the positron fraction based on data from the Lynn Lake flight, and compare these with the previously published results from the Ft. Sumner flight. The results confirm that the positron fraction does not increase with energy above ~10 GeV, although a small excess above purely secondary production cannot be ruled out. At low energies the positron fraction is slightly larger than that reported from measurements made in the 1960's. This effect could possibly be a consequence of charge dependence in the level of solar modulation.Comment: 7 pages, LaTeX, two eps figures, aas2pp4.sty and epsf.tex needed, accepted by Ap.J. Lett. (March 27, 1997

The AMANDA Neutrino Telescope and the Indirect Search for Dark Matter

With an effective telescope area of order 10^4 m^2, a threshold of ~50 GeV and a pointing accuracy of 2.5 degrees, the AMANDA detector represents the first of a new generation of high energy neutrino telescopes, reaching a scale envisaged over 25 years ago. We describe its performance, focussing on the capability to detect halo dark matter particles via their annihilation into neutrinos.Comment: Latex2.09, 16 pages, uses epsf.sty to place 15 postscript figures. Talk presented at the 3rd International Symposium on Sources and Detection of Dark Matter in the Universe (DM98), Santa Monica, California, Feb. 199

Limits to the muon flux from WIMP annihilation in the center of the Earth with the AMANDA detector

A search for nearly vertical up-going muon-neutrinos from neutralino annihilations in the center of the Earth has been performed with the AMANDA-B10 neutrino detector. The data sample collected in 130.1 days of live-time in 1997, ~10^9 events, has been analyzed for this search. No excess over the expected atmospheric neutrino background is oberved. An upper limit at 90% confidence level on the annihilation rate of neutralinos in the center of the Earth is obtained as a function of the neutralino mass in the range 100 GeV-5000 GeV, as well as the corresponding muon flux limit.Comment: 14 pages, 11 figures. Version accepted for publication in Physical Review

Coevolved mutations reveal distinct architectures for two core proteins in the bacterial flagellar motor

Switching of bacterial flagellar rotation is caused by large domain movements of the FliG protein triggered by binding of the signal protein CheY to FliM. FliG and FliM form adjacent multi-subunit arrays within the basal body C-ring. The movements alter the interaction of the FliG C-terminal (FliGC) "torque" helix with the stator complexes. Atomic models based on the Salmonella entrovar C-ring electron microscopy reconstruction have implications for switching, but lack consensus on the relative locations of the FliG armadillo (ARM) domains (amino-terminal (FliGN), middle (FliGM) and FliGC) as well as changes during chemotaxis. The generality of the Salmonella model is challenged by the variation in motor morphology and response between species. We studied coevolved residue mutations to determine the unifying elements of switch architecture. Residue interactions, measured by their coevolution, were formalized as a network, guided by structural data. Our measurements reveal a common design with dedicated switch and motor modules. The FliM middle domain (FliMM) has extensive connectivity most simply explained by conserved intra and inter-subunit contacts. In contrast, FliG has patchy, complex architecture. Conserved structural motifs form interacting nodes in the coevolution network that wire FliMM to the FliGC C-terminal, four-helix motor module (C3-6). FliG C3-6 coevolution is organized around the torque helix, differently from other ARM domains. The nodes form separated, surface-proximal patches that are targeted by deleterious mutations as in other allosteric systems. The dominant node is formed by the EHPQ motif at the FliMMFliGM contact interface and adjacent helix residues at a central location within FliGM. The node interacts with nodes in the N-terminal FliGc α-helix triad (ARM-C) and FliGN. ARM-C, separated from C3-6 by the MFVF motif, has poor intra-network connectivity consistent with its variable orientation revealed by structural data. ARM-C could be the convertor element that provides mechanistic and species diversity.JK was supported by Medical Research Council grant U117581331. SK was supported by seed funds from Lahore University of Managment Sciences (LUMS) and the Molecular Biology Consortium