Human immunodeficiency virus (HIV) infection in tuberculosis patients in Addis Ababa

BACKGROUND: In a country with a rapidly spreading HIV epidemic information regarding HIV and TB Co-infection are lacking. OBJECTIVES: To determine the prevalence of HIV infection in a representative sample of sputum-positive tuberculosis patients. METHODS: A cross-sectional survey whereby blood sample was collected from 236 consecutively coming smear positive pulmonary tuberculosis patients for HIV testing. This study, which involved all the health centres in Addis Ababa, was carried out during August 1, 1998 to the end of December 1998. RESULTS: Of the 236 blood samples collected, 107(45.3%) were HIV positive. Among the HIV positives, 66 (61.7%) were male and 41(38.3%) females. The HIV-TB co-infection was highest in the age group 20-49 and the largest number of TB co-infection (75% of all such co-infection) was found in the 20-39 age group. There was no significant difference between the HIV positive and negative TB patients concerning to other socio-demographic factors or presenting symptoms. CONCLUSION: The prevalence of co-infection appeared to have increased compared to previous studies, 6.6%, 20 & 25% and 44.4% in 1990, 1995, and 1996, respectively. This trend may have a serious impact on the control of tuberculosis. Co-ordinating strategies of the TB and HIV control programs is recommended. (Ethiopian Journal of Health Development, 2000, 14(3): 277-282

Diversion of the melanin synthetic pathway by dopamine product scavengers: A quantum chemical modeling of the reaction mechanisms

We report the stability and reactivity of the oxidation products as well as L-cysteine and N-acetylcysteine adducts of dopamine studied using quantum chemical calculations. The overall reactions studied were subdivided into four reaction channels. The first reaction channel is the oxidation of dopamine to form dopaminoquinone. The second reaction channel leads to melanin formation through subsequent reactions. The third and fourth reaction channels are reactions leading to the formation of dopaminoquinone adducts which are aimed to divert the synthesis of melanin. The results indicate that L-cysteine and N-acetylcysteine undergo chemical reactions mainly at C5 position of dopaminoquinone. The analyses of the thermodynamic energies indicate that L-cysteine and N-acetylcysteine covalently bind to dopaminoquinone by competing with the internal cyclization reaction of dopaminoquinone which leads to the synthesis of melanin. The analysis of the results, based on the reaction free energies, is also supported by the investigation of the natural bond orbitals of the reactants and products. KEY WORDS: Dopamine, Melanin, L-Cysteine, N-Acetylcysteine, DFT calculations Bull. Chem. Soc. Ethiop. 2016, 30(3), 437-448DOI: http://dx.doi.org/10.4314/bcse.v30i3.1

Enhancing first year chemistry student’s participation in practical chemistry course

In this study, enhancing student’s participation in practical analytical chemistry course at Haramaya University with various reasons was conducted. The data were collected from I year chemistry undergraduate students of class size 56 of which 23 were females and 33 were males. The class was arranged in to two groups for laboratory class and the experiment was conducted once per week in analytical laboratory. The research used mainly three kinds of data collection techniques namely questionnaire and laboratory report and demonstration result to gather the required qualitative and quantitative data for improving the participation of first year chemistry students. From the result, it was found that students were interested towards the practical analytical courses on the basis of condition such as, necessity of the chemistry with life, their participation in the laboratory, getting experience from the laboratory and performing the experiment in group. The results of the study support the notion that, students were more interested to a group work rather than individual work since they share idea, read their manual before coming to laboratory which contributed to their own participation in learning practical chemistry courses. The research has also shown that student’s activity through experimental demonstration in group increases student’s participations in the laboratory effectively by achieving better results than using laboratory report writing methods. The findings also revealed that some of the causes of students’ negative attitudes towards learning practical Chemistry were mainly due to problems in preparing a flow chart for the experiments by themselves and lack of exposure to well-equipped laboratory for conducting demonstrations. In view of the findings and conclusions drawn in the study, Chemistry laboratories should be adequately equipped to ensure a smooth running of the practical classes and students should be encouraged to participate on practical chemistry courses and appropriate motivation should be given so that they will develop positive attitude towards the practical sessions

Mercury Methylation by Cobalt Corrinoids: Relativistic Effects Dictate the Reaction Mechanism

The methylation of HgII(SCH3)2 by corrinoid‐based methyl donors proceeds in a concerted manner through a single transition state by transfer of a methyl radical, in contrast to previously proposed reaction mechanisms. This reaction mechanism is a consequence of relativistic effects that lower the energies of the mercury 6p1/2 and 6p3/2 orbitals, making them energetically accessible for chemical bonding. In the absence of spin–orbit coupling, the predicted reaction mechanism is qualitatively different. This is the first example of relativity being decisive for the nature of an observed enzymatic reaction mechanism.Of relative importance: The methylation of HgII(SCH3)2 by corrinoid‐based methyl donors proceeds in a concerted manner through a single transition state by transfer of a methyl radical. This reaction mechanism is a consequence of relativistic effects, and constitutes the first example of relativity being decisive for the nature of an enzymatic reaction mechanism. SOC=spin–orbit coupling.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/137374/1/anie201606001-sup-0001-misc_information.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/137374/2/anie201606001.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/137374/3/anie201606001_am.pd

Longer delays in diagnosis and treatment ofpulmonary tuberculosis in pastoralist setting, Eastern Ethiopia

Purpose: This study aimed to assess the extent of patient, health system and total delays in diagnosis and treatment of pulmonary tuberculosis (TB) in Somali pastoralist setting, Ethiopia. Patients and Methods: A cross-sectional study among 444 confirmed new pulmonary TB patients aged ≥15 years in 5 TB care units was conducted between December 2017 and October 2018. Data were collected using a structured questionnaire and record review. We measured delays from symptom onset to provider visit, provider visit to diagnosis and diagnosis to treatment initiation. Delays were summarized using median days. Mann- Whitney and Kruskal-Wallis tests were used to compare delays between categories of explanatory variables. The Log-binomial regression model was used to reveal factors associated with health system delay ≥15 days, presented in adjusted prevalence ratio (APR) with 95% confidence interval (CI). Results: The median age of patients was 30 years, ranged from 15 to 82. The majority (62.4%) were male, and nearly half (46.4%) were pastoralists. The median patient, health system and total delays were 30 (19-48.5), 14 (4.5-29.5) and 50 (35-73.5) days, respec-tively. The median patient delay (35.5 days) and total delay (58.5 days) among pastoralists were substantially higher than the equivalent delays among non-pastoralists [p<0.001]. Of all, 3.8% of patients (16 of 18 were pastoralists) delayed longer than 6 months without initiating treatment. Factors associated with health system delay ≥15 days were mild symptoms [APR (95% CI) = 1.4 (1.1-1.7)], smear-negativity [APR (95% CI) = 1.2 (1.01- 1.5)], first visit to health centers [APR (95% CI) = 1.6 (1.3-2.0)] and multiple provider contacts [APR (95% CI) = 5.8 (3.5-9.6)]. Conclusion: Delay in diagnosis and treatment remains a major challenge of tuberculosis control targets in pastoralist settings of Ethiopia. Efforts to expand services tailored to transhumance patterns and diagnostic capacity of primary healthcare units need to be prioritized. © 2020 Getnet et al.Purpose: This study aimed to assess the extent of patient, health system and total delays in diagnosis and treatment of pulmonary tuberculosis (TB) in Somali pastoralist setting, Ethiopia. Patients and Methods: A cross-sectional study among 444 confirmed new pulmonary TB patients aged ≥15 years in 5 TB care units was conducted between December 2017 and October 2018. Data were collected using a structured questionnaire and record review. We measured delays from symptom onset to provider visit, provider visit to diagnosis and diagnosis to treatment initiation. Delays were summarized using median days. Mann- Whitney and Kruskal-Wallis tests were used to compare delays between categories of explanatory variables. The Log-binomial regression model was used to reveal factors associated with health system delay ≥15 days, presented in adjusted prevalence ratio (APR) with 95% confidence interval (CI). Results: The median age of patients was 30 years, ranged from 15 to 82. The majority (62.4%) were male, and nearly half (46.4%) were pastoralists. The median patient, health system and total delays were 30 (19-48.5), 14 (4.5-29.5) and 50 (35-73.5) days, respec-tively. The median patient delay (35.5 days) and total delay (58.5 days) among pastoralists were substantially higher than the equivalent delays among non-pastoralists [p<0.001]. Of all, 3.8% of patients (16 of 18 were pastoralists) delayed longer than 6 months without initiating treatment. Factors associated with health system delay ≥15 days were mild symptoms [APR (95% CI) = 1.4 (1.1-1.7)], smear-negativity [APR (95% CI) = 1.2 (1.01- 1.5)], first visit to health centers [APR (95% CI) = 1.6 (1.3-2.0)] and multiple provider contacts [APR (95% CI) = 5.8 (3.5-9.6)]. Conclusion: Delay in diagnosis and treatment remains a major challenge of tuberculosis control targets in pastoralist settings of Ethiopia. Efforts to expand services tailored to transhumance patterns and diagnostic capacity of primary healthcare units need to be prioritized. © 2020 Getnet et al

Integration of family planning services with HIV treatment for women of reproductive age attending ART clinic in Oromia regional state, Ethiopia

Abstract Background In settings where HIV prevalence is high, management of sexual and reproductive health is critical to reducing HIV transmission and maternal mortality. Integration of family planning with HIV services is appropriate for HIV therapy, HIV prevention, and care in a resource-limited country s like Ethiopia. The study aimed at examining the status of integration of family planning services with HIV treatment and factors associated with successful integration of family planning and HIV services for women of reproductive age in Oromia, Ethiopia for better health outcomes. Methods The research design of this study was a quantitative survey, non-experimental, explorative and descriptive. A questionnaire was used to collect data from women living with HIV attending ART clinics in the special zone of surrounding Finfinne, Oromia Region in five health centers. Simple random sampling was used to select 654 respondents. Data was analysed through the use of Statistical Package for Social Sciences version 23.0. Bivariate and multivariate logistic regressions were performed to identify factors associated integration of family planning with HIV services with the significant association at an adjusted odds ratio (AOR) with a 95% confidence interval (CI) to controlled effects of possible confounders from the final model. Result The response rate of this study was 97.6% (654/670). The ages of those who responded to the administered questionnaires ranged between 18 and 49 years. The mean age of the respondents was 31.86 years with an SD of ± 6.0 years. Most of the respondents in the sample were in the age group 26–35 (n = 374, 57%), and only 96 (14.7%) were in the age group 18–25. This overall integration of FP-HIV services among reproductive-age women living with HIV in Oromia regional state of special zone health centers was found to be 55.8%. Almost all respondents (n = 635, 97.1%) preferred integrated family planning and HIV services from the same facility and the same providers. the study found that 622 (95%) were most satisfied with the utilization of integrated family planning/HIV services. Conclusion This study established that in overall, the integration of family planning/HIV services was relatively moderate among women of reproductive age living HIV. The identified factors that affected the integration of family planning with HIV services were the level of education, occupational status, residence, discussion of family planning with healthcare providers, fertility desire and CD4 counts.Plain language summary These study findings are strongly in favour of integrated services offering multiple health services at the same facility by the same provider. Service integration based on the needs of women of reproductive age was found to be necessary in public health facilities where people have access only to primary healthcare facilities. Integrated family planning with HIV (FP-HIV) services contribute to women’s wellbeing by addressing a wide range of health needs and offering convenience of receiving multiple services a single visit. Integrated FP-HIV services further address women’s vulnerability to other sexual reproductive health problems. Integrated FP-HIV services should contribute to comprehensive national family planning programs to enable the provision of full access to a variety of contraceptive methods. The latter should enable couples and individuals to obtain services at a one-stop supermarket to achieve optimal reproductive and sexual health to meet their needs. The findings from study generated adequate knowledge and equipped managers with skills integrating family planning with HIV services among women of reproductive age in Oromia regional state, Ethiopia. The study’s findings have emphasized the urgent need to fully implement action focusing on integrating family planning/HIV services for women of reproductive age living HIV and attending ART clinics. The study prioritized the need to strengthen the capacity of health centers to provide quality FP-HIV

Challenges in delivery of tuberculosis services in Ethiopian pastoralist settings: clues for reforming service models and organizational structures

BACKGROUND: The End-TB strategy aims to see a world free of tuberculosis (TB) by the coming decade through detecting and treating all cases irrespective of socioeconomic inequalities. However, case detections and treatment outcomes have not been as they should be in Somali pastoral settings of Ethiopia. Hence, this study aimed to explore the challenges that hinder the delivery and utilization of TB services in pastoral areas. METHODS: A qualitative study was conducted between December 2017 and October 2018 among pastoralist patients with delay of >/=2 months in seeking healthcare, healthcare providers and programme managers. Data were collected from different sources using 41 in-depth interviews, observations of facilities and a review meeting of providers from 50 health facilities. The data were transcribed, coded and analyzed to identify pre-defined and emerging sub-themes. ATLAS.ti version 7.0 was used for coding data, categorizing codes, and visualizing networks. RESULTS: Poor knowledge of TB and its services, limited accessibility (unreachability, unavailability and unacceptability), pastoralism, and initial healthcare-seeking at informal drug vendors that provide improper medications were the key barriers hindering the uptake of TB medical services. Inadequate infrastructure, shortage of trained and enthused providers, interruptions of drugs and laboratory supplies, scarce equipment, programme management gaps, lack of tailored approach, low private engagement, and cross-border movement were the major challenges affecting the provision of TB services for pastoral communities. The root factors were limited potential healthcare coverage, lack of zonal and district TB units, mobility and drought, strategy and funding gaps, and poor development infrastructure. CONCLUSION: In pastoral settings of Ethiopia, the major challenges of TB services are limited access, illicit medication practices, inadequate resources, structural deficits, and lack of tailored approaches. Hence, for the pastoral TB control to be successful, mobile screening and treatment modalities and engaging rural drug vendors will be instrumental in enhancing case findings and treatment compliance; whereas, service expansion and management decentralization will be essential to create responsive structures for overcoming challenges

Spatiotemporal variability of soil moisture over Ethiopia and its teleconnections with remote and local drivers

Soil moisture is one of the essential climate variables with a potential impact on local climate variability. Despite the importance of soil moisture, studies on soil moisture characteristics in Ethiopia are less documented. In this study, the spatiotemporal variability of Ethiopian soil moisture (SM) has been characterized, and its local and remote influential driving factors are investigated. An empirical orthogonal function (EOF) and KMeans clustering algorithm have been employed to classify the large domain into homogeneous zones. Complex maximum covariance analysis (CMCA) is applied to evaluate the covariability between SM and selected local and remote variables such as rainfall (RF), evapotranspiration (ET), and sea surface temperature (SST). Inter-comparison among SM datasets highlight that the FLDAS dataset better depicts the country’s SM spatial and temporal distribution (i.e., a correlation coefficient r=0.95 , rmsd=0.04m3m−3 with observations). Results also indicate that regions located in northeastern Ethiopia are drier irrespective of the season (JJAS, MAM, and OND) considered. In contrast, the western part of the country consistently depicted a wetter condition in all seasons. During summer (JJAS), the soil moisture variability is characterized by a strong east–west spatial contrast. The highest and lowest soil moisture values were observed across the country’s central western and eastern parts, respectively. Furthermore, analyses indicate that interannual variability of SM is dictated substantially by RF, though the impact on some regions is weaker. It is also found that ET likely drives the SM in the eastern part of Ethiopia due to a higher atmospheric moisture demand that ultimately invokes changes in surface humidity and rainfall. A composite analysis based on the extreme five wettest and driest SM years revealed a similar spatial distribution of wet SM with positive anomalies of RF across the country and ET over the southern regions. Remote SSTs are also found to have a significant influence on SM distribution. In particular, equatorial central Pacific and western Indian oceans SST anomalies are predominant factors for spatiotemporal SM variations over the country. Major global oceanic indices: Oceanic Nino Index (ONI), Indian Ocean Dipole (IOD), Pacific warm pool (PACWARMPOOL), and Pacific Decadal Oscillations (PDO) are found to be closely associated with the SM anomalies in various parts of the country. The associationship between these remote SST anomalies and local soil moisture is via large-scale atmospheric circulations that are linked to regional factors such as precipitation and temperature anomalies.publishedVersio

Limekiln Slough Drainage, Pulaski County, Illinois

published or submitted for publicationis peer reviewedOpe