Morphological and molecular characterization of a new species of Isospora Schneider, 1881 (Apicomplexa: Eimeriidae) from the western wattlebird Anthochaera lunulata Gould in Western Australia

A new coccidian species, Isospora lunulatae n. sp., from the western wattlebird Anthochaera lunulata Gould in Western Australia is described and characterised molecularly. Microscopic analysis of a faecal sample identified subspheroidal oöcysts measuring 27–34 × 26–31 (30.6 × 29.4) μm (n = 20), with a length/width (L/W) ratio of 1.0–1.1 (1.0). Oöcysts have a bi-layered wall, 0.9–1.2 (1.0) μm thick; the outer layer is smooth, representing c.2/3 of total thickness. Micropyle and oöcyst residuum are both absent, but a polar granule is present. Sporocysts are ovoidal, 17–19 × 10–12 (18.3 × 10.7) μm, with a L/W ratio of 1.6–1.8 (1.7) and occupying about 21% of the area (each one) within the oöcyst. Stieda body is flattened to rounded, measuring on average 0.9 × 1.8 μm; sub-Stieda body is rounded to rectangular, measuring on average 1.5 × 2.6 μm; para-Stieda body is absent. Sporocyst residuum has an irregular shape consisting of numerous granules and appears membrane-bound. Sporozoites are vermiform 12.8 × 3.0 μm on average, with prominent striations at the more pointed end and two refractile bodies below striations. Segments of three gene loci (18S rRNA, 28S rRNA and cox1) were sequenced and I. lunulatae n. sp. exhibited 99.6% genetic similarity to Isospora phylidonyrisae Yang, Brice, Berto & Ryan, 2021 at the 18S rRNA gene locus, 99.8% genetic similarity to Isospora anthochaerae Yang, Brice & Ryan, 2014 and shared a 98.1% genetic similarity with Isospora manorinae Yang, Brice, Jian & Ryan, 2016 at the cox1 gene locus. Morphological and molecular data support the distinct species status of the new species

Combinations of isoform-targeted histone deacetylase inhibitors and bryostatin analogues display remarkable potency to activate latent HIV without global T-cell activation

AbstractCurrent antiretroviral therapy (ART) for HIV/AIDS slows disease progression by reducing viral loads and increasing CD4 counts. Yet ART is not curative due to the persistence of CD4+ T-cell proviral reservoirs that chronically resupply active virus. Elimination of these reservoirs through the administration of synergistic combinations of latency reversing agents (LRAs), such as histone deacetylase (HDAC) inhibitors and protein kinase C (PKC) modulators, provides a promising strategy to reduce if not eradicate the viral reservoir. Here, we demonstrate that largazole and its analogues are isoform-targeted histone deacetylase inhibitors and potent LRAs. Significantly, these isoform-targeted HDAC inhibitors synergize with PKC modulators, namely bryostatin-1 analogues (bryologs). Implementation of this unprecedented LRA combination induces HIV-1 reactivation to unparalleled levels and avoids global T-cell activation within resting CD4+ T-cells.</jats:p

Ultracompact and unidirectional metallic antennas

International audienceWe investigate the angular redistribution of light radiated by a single emitter located in the vicinity of dipolar silver nanoparticles. We point out the fundamental role of the phase differences introduced by the optical path difference between the emitter and the particle and demonstrate that the polarizability of the metallic nanoparticle alone cannot predict the emission directionality. In particular, we show that collective or reflective properties of single nanoparticles can be controlled by tuning the distance of a single emitter at a λ/30 scale. These results enable us to design unidirectional and ultracompact nanoantennas composed of just two coupled nanoparticles separated by a distance achievable with biological linkers

Eimeria collieie n. sp. (Apicomplexa:Eimeriidae) from the western long-necked turtle (Chelodina colliei)

A new species, Eimeria collieie n. sp., is described from the western long-necked turtle (Chelodina colliei). Sporulated oocysts (n = 35) are spherical to subspherical, with colourless single layer oocyst wall, 0.6 ± 0.2 (0.4-0.7) μm thick. Oocyst with elongated ellipsoid sporocysts. Oocyst length, 29.8 ± 0.4 (28.2-31.0) μm; oocyst width, 29.4 ± 0.3 (28.0-30.8) μm; oocyst length/width (L/W) ratio, 1.0 ± 0.03 (1.0-1.05). Micropyle, oocyst residuum and polar granule were absent. Sporocysts with sporocyst residuum and 2 sporozoites. Sporocyst length, 21.6 ± 0.4 (21.2-22.0) μm; sporocyst width, 6.0 ± 0.3 (5.7-6.3) μm; sporocyst L/W ratio, 3.6 ± 0.2 (3.4-3.8). Stieda, parastieda and substieda bodies were absent. Sporozoite length, 14.0 ± 0.2 (13.8-14.2) μm; sporozoite width, 2.6 ± 0.2 (2.4-2.8) μm; sporozoite L/W ratio, 5.46 ± 0.10 (5.4-5.6). Molecular analysis was conducted at three loci: the 18S and 28S ribosomal RNA (rRNA), and the mitochondrial cytochrome oxidase gene (COI). At the 18S rRNA locus, E. collieie n. sp. shared 96.4% and 98.3% genetic similarity to E. ranae (GenBank accession number: EU717219) and E. arnyi (AY613853) respectively. At the 28S rRNA locus, E. collieie n. sp. shared 91.6% genetic similarity to E. papillata (GenBank accession number: GU593706) and phylogenetic analysis at this locus placed E. collieie n. sp. in aseparateclade. At the COI locus, E. collieie n. sp. shared 92.7% genetic similarity to Eimeria setonicis (GenBankaccession number: KF225638) from a quokka (Setonix brachyurus) in Western Australia. Reptile-derived sequences were not available for the 28S rRNA and the COI loci. Based on morphological and molecular data, this isolate is a new species of coccidian parasite that, to date, has only been found in western long-necked turtles

Pression Agrofonciere Et Mutations Agraires Sur Le Plateau Adja Au Sud Ouest Du Benin

History shows that the evolution of agrarian systems has generally been towards more intensive systems under the pressure of demographic pressure. The agrofoncial pressure experienced by the Adja Plateau and the resulting agrarian mutations have been studied by this research. The methodology used includes data collection, processing and analysis. The socio-economic surveys were carried out in selected villages on the basis of well-defined criteria. The 1995 Schwartz method was used to establish the sample. Also, seven (7) focus groups were carried out in 7 different villages. The proportions of arable land per farm and land tenure were calculated and analyzed. Agricultural practices were identified and then characterized. The determination of the level of pressure on the land was carried out by calculating the coefficients of Rutemberg R (1980), Allan L (1965) and the agro-demographic index (IAT). The results show that, in addition to traditional modes of access to arable land, such as inheritance and donation, new modes such as sharecropping, leasing and purchase have emerged and developed on the Adja Plateau. This situation can be explained by the gradual reduction of land available for agricultural assets, with an average of 0.34 ha for the whole of the Plateau. The result is a very long period of land use (9 years) before set-aside. For the whole Plateau, the average values of R, L and AIT are respectively 78.39; 1.28 and 0.12 ha / hbt. These figures indicate that the environment is characterized by a system of permanent crops in an area subject to high human pressure

Gravitational Lensing as Signal and Noise in Lyman-alpha Forest Measurements

In Lyman-alpha forest measurements it is generally assumed that quasars are mere background light sources which are uncorrelated with the forest. Gravitational lensing of the quasars violates this assumption. This effect leads to a measurement bias, but more interestingly it provides a valuable signal. The lensing signal can be extracted by correlating quasar magnitudes with the flux power spectrum and with the flux decrement. These correlations will be challenging to measure but their detection provides a direct measure of how features in the Lyman-alpha forest trace the underlying mass density field. Observing them will test the fundamental hypothesis that fluctuations in the forest are predominantly driven by fluctuations in mass, rather than in the ionizing background, helium reionization or winds. We discuss ways to disentangle the lensing signal from other sources of such correlations, including dust, continuum and background residuals. The lensing-induced measurement bias arises from sample selection: one preferentially collects spectra of magnified quasars which are behind overdense regions. This measurement bias is ~0.1-1% for the flux power spectrum, optical depth and the flux probability distribution. Since the effect is systematic, quantities such as the amplitude of the flux power spectrum averaged across scales should be interpreted with care.Comment: 22 pages, 8 figures; v2: references added, discussion expanded, matches PRD accepted versio

Eimeria spp. and Tyzzeria perniciosa Allen, 1936 (Apicomplexa: Eimeriidae) from a Pacific black duck, Anas superciliosa Gmelin (Aves: Anseriformes), in western Australia

Four species of the Eimeriidae, Eimeria anatis Scholtyseck, 1955, Eimeria aythyae Farr, 1965, Eimeria krylovi Svanbaev & Rakhmatullina, 1967 and Tyzzeria perniciosa Allen, 1936, were morphologically identified from oöcysts recovered from a Pacific black duck, Anas superciliosa Gmelin. Additionally, genotypic characterization of E. anatis is provided via sequencing of the mitochondrial cytochrome c oxidase subunit 1 (cox1) and the small subunit ribosomal RNA (18S) genes. The four species are redescribed, providing additional morphological details. The validity of genera and coccidian species parasitizing birds of the order Anseriformes such as Wenyonella Hoare, 1933 and some Tyzzeria spp. are discussed. Molecular phylogenetic analyses for the cox1 and 18S rRNA genes resulted in monophylies of Eimeria spp. from Anseriformes which included the sequences obtained from E. anatis oöcysts

Reviewing Fire, Climate, Deer, and Foundation Species as Drivers of Historically Open Oak and Pine Forests and Transition to Closed Forests

Historically open oak and pine savannas and woodlands have transitioned to closed forests comprised of increased numbers of tree species throughout the eastern United States. We reviewed evidence for and against a suite of previously postulated drivers of forest transition focused on (1) change in fire regimes, (2) increased precipitation, (3) increased white-tailed deer densities, and (4) loss of American chestnut. We found that fire and fire exclusion provide a parsimonious mechanism for historical dominance by open forests of fire-tolerant oak and/or pine species and subsequent transition to closed forests with fire-sensitive tree species that fill the vertical profile. Based on statistical tests, increased precipitation during the past century was within historical ranges and thus fails to provide an explanation for forest change; additionally, precipitation variability is incongruent with tree traits (i.e., both drought-tolerant and drought-intolerant species have increased and decreased) and patterns of tree establishment. Similarly, current deer densities fail to provide a statistical relationship to explain tree densities at regional scales, species trends are unrelated to deer browse preferences, and both historically open forests and contemporary closed forests contained high deer densities. Functional extinction of the American chestnut had localized impacts but chestnut was not abundant compared to oak or widespread enough in distribution to match forest transitions throughout the eastern United States. Although Euro-American settlement affected many processes, not all changes were consistent enough to cause transitions in forest composition and structure that generally trailed westward expansion by Euro-American settlers. Evidence about these drivers continues to mount and we recognize the need for further research and continual re-evaluation of drivers of historical forests and forest change due to importance for understanding and management of these ecosystems

A functional non-central limit theorem for jump-diffusions with periodic coefficients driven by stable Levy-noise

We prove a functional non-central limit theorem for jump-diffusions with periodic coefficients driven by strictly stable Levy-processes with stability index bigger than one. The limit process turns out to be a strictly stable Levy process with an averaged jump-measure. Unlike in the situation where the diffusion is driven by Brownian motion, there is no drift related enhancement of diffusivity.Comment: Accepted to Journal of Theoretical Probabilit

Spatial mapping and prediction of Plasmodium falciparum infection risk among school-aged children in Côte d'Ivoire

BACKGROUND: In Côte d'Ivoire, malaria remains a major public health issue, and thus a priority to be tackled. The aim of this study was to identify spatially explicit indicators of Plasmodium falciparum infection among school-aged children and to undertake a model-based spatial prediction of P. falciparum infection risk using environmental predictors. METHODS: A cross-sectional survey was conducted, including parasitological examinations and interviews with more than 5,000 children from 93 schools across Côte d'Ivoire. A finger-prick blood sample was obtained from each child to determine Plasmodium species-specific infection and parasitaemia using Giemsa-stained thick and thin blood films. Household socioeconomic status was assessed through asset ownership and household characteristics. Children were interviewed for preventive measures against malaria. Environmental data were gathered from satellite images and digitized maps. A Bayesian geostatistical stochastic search variable selection procedure was employed to identify factors related to P. falciparum infection risk. Bayesian geostatistical logistic regression models were used to map the spatial distribution of P. falciparum infection and to predict the infection prevalence at non-sampled locations via Bayesian kriging. RESULTS: Complete data sets were available from 5,322 children aged 5-16 years across Côte d'Ivoire. P. falciparum was the predominant species (94.5 %). The Bayesian geostatistical variable selection procedure identified land cover and socioeconomic status as important predictors for infection risk with P. falciparum. Model-based prediction identified high P. falciparum infection risk in the north, central-east, south-east, west and south-west of Côte d'Ivoire. Low-risk areas were found in the south-eastern area close to Abidjan and the south-central and west-central part of the country. CONCLUSIONS: The P. falciparum infection risk and related uncertainty estimates for school-aged children in Côte d'Ivoire represent the most up-to-date malaria risk maps. These tools can be used for spatial targeting of malaria control interventions