Formal Verification Integration Approach for DSML

International audienceThe application of formal methods (especially, model check- ing and static analysis techniques) for the verification of safety critical embedded systems has produced very good results and raised the inter- est of system designers up to the application of these technologies in real size projects. However, these methods usually rely on specific verifica- tion oriented formal languages that most designers do not master. It is thus mandatory to embed the associated tools in automated verification toolchains that allow designers to rely on their usual domain-specific modeling languages (DSMLs) while enjoying the benefits of these power- ful methods. More precisely, we propose a language to formally express system requirements and interpret verification results so that system designers (DSML end-users) avoid the burden of learning some formal verification technologies. Formal verification is achieved through trans- lational semantics. This work is based on a metamodeling pattern for executable DSML that favors the definition of generative tools and thus eases the integration of tools for new DSML

Plasmodium falciparum metacaspase PfMCA-1 triggers a z-VAD-fmk inhibitable protease to promote cell death.

Activation of proteolytic cell death pathways may circumvent drug resistance in deadly protozoan parasites such as Plasmodium falciparum and Leishmania. To this end, it is important to define the cell death pathway(s) in parasites and thus characterize proteases such as metacaspases (MCA), which have been reported to induce cell death in plants and Leishmania parasites. We, therefore, investigated whether the cell death function of MCA is conserved in different protozoan parasite species such as Plasmodium falciparum and Leishmania major, focusing on the substrate specificity and functional role in cell survival as compared to Saccharomyces cerevisae. Our results show that, similarly to Leishmania, Plasmodium MCA exhibits a calcium-dependent, arginine-specific protease activity and its expression in yeast induced growth inhibition as well as an 82% increase in cell death under oxidative stress, a situation encountered by parasites during the host or when exposed to drugs such as artemisins. Furthermore, we show that MCA cell death pathways in both Plasmodium and Leishmania, involve a z-VAD-fmk inhibitable protease. Our data provide evidence that MCA from both Leishmania and Plasmodium falciparum is able to induce cell death in stress conditions, where it specifically activates a downstream enzyme as part of a cell death pathway. This enzymatic activity is also induced by the antimalarial drug chloroquine in erythrocytic stages of Plasmodium falciparum. Interestingly, we found that blocking parasite cell death influences their drug sensitivity, a result which could be used to create therapeutic strategies that by-pass drug resistance mechanisms by acting directly on the innate pathways of protozoan cell death

Model-Based Code Generation

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Using AADL to build critical real-time systems: Experiments in the IST-ASSERT project

International audienceIn this paper, we discuss the use of the AADL (Architecture Analysis and Design Language) in the IST- ASSERT project, which spans from 2004 to 2007. In the context of this project, the European Space Agency, in collaboration with tool providers and academic partners explored the use of AADL to build space systems. At the completion of the project, we provide our report and experiments on AADL for building critical systems

Tuberculosis lymphadenitis in a south-eastern region in Tunisia: Epidemiology, clinical features, diagnosis and treatment

Aim: To evaluate patients’ profiles, demographics, clinical and therapeutic approaches and strategies in patients with tuberculous lymphadenitis (TBG). Patients and methods: A retrospective study of all TBG-confirmed cases admitted in a tuberculosis specific health care facility between 1 January 2009 and 16 June 2013. Results: A total of 181 clinical files were examined. Mean age was 32 years old; the female/male ratio was 1.78 to 1. Raw milk consumption was noted in 1/3 of patients. Most cases involved the head and neck region (83.4%), nodes involvement, including axillary (12 cases), and mediastinal (9 cases). Clinical symptoms were present in only 55.2%. TST was conducted with 82.6% positive responses. Diagnostics confirmation was done with anatomical pathology in most of the patients; only 56 of them had any microbiology analysis done. Demonstration of acid-fast bacilli in microscopy from either fine-needle aspirates or biopsies was done in 17.5%, and cultures yielded positive results in 27%. Treatment duration was varied. Paradoxical reactions were noted in 12% and persistent lymphadenopathy after treatment completion was noted in 10% of cases. Conclusions: TBG remains a disease of interest. Today, its diagnosis and management is still a problem despite its increasing worldwide incidence, and especially in this study area. Disease control should be strengthened in this country