Anti-inflammatory and antioxidant properties of Eriobotrya japonica leaves extracts

Background: In the present work we determined phenolic and flavonoids content of Eriobotrya japonica leaves extracts and fractions and their antioxidant and anti-inflammatory properties.Objectives: To evaluate the inhibition of inflammatory PLA2 and antioxidant effects of extracts and fractions from Eriobotrya japonica leavesMethods: Antioxidant activity was evaluated with DPPH radical scavenging assay and anti-inflammatory effect of fractions was measured by their inhibition potency on the human pro-inflammatory phospholipase A2 (group IIA).Results: The EtOH/EtOAc 2:1 extract exhibited a potent inhibition of the hG-IIA with an IC50 values of 8 μg/ml. It also shows an antioxidant activity measured on DPPH with an IC50 of 42 μg/ml. Fractionation shows that CH2Cl2/MeOH 0:1 fraction was the rich one on flavonoids compounds (4.3 mg/g dry weight) and demonstrates a high antioxidant activity with an IC50 of 12 μg/ml. The anti-inflammatory evaluation demonstrates that the same fraction was the best one to inhibit the pro-inflammatory phospholipase A2 group IIA with an IC50 of 4 μg/ml.Conclusion: Study conducted on Eriobotrya japonica shows that CH2Cl2/MeOH 0:1 fraction inhibits efficiently the hGIIA phospholipase.which is considered as pro-inflammatory enzyme.Keywords: Eriobotrya japonica, extraction, flavonoids, anti-inflammatory

Refugees Hosting Other refugees: Endurance and Maintenance of Care in Ouzaii (Lebanon)

This article examines the socio-spatial mechanisms that emerge when refugees host other refugees. It argues that there is an underlying social infrastructure of care that impacts the refugees’ choice of destinations and modes of survival. When refugees host other refugees from close networks of relatives and neighbours, they create their own spatial clusters. In the process, the social infrastructure of care offers one mode of security to vulnerable refugees. Care as a concept and an approach is related to ideas of endurance and maintenance. It facilitates multiple dimensions, from space, to affection and to the everyday. It is able to reconfigure a life possible, life-enduring and a life meaningful in an urban setting. We focus on Ouzaii in Beirut, Lebanon. Ouzaii has been a destination for multiple displaced groups over different periods of time. Ouzaii currently hosts an approximate 10,000 Syrian refugees. They chose Ouzaii as their destination after they were helped by existing refugees who offered shelter and access to jobs. The resultant socio-spatial practices, flourishing businesses and leisurely facilities are evidence of successful social networks that form an infrastructure of care. They also play a role in the reconstitution of Ouzaii itself. We conclude with reflections on how urban informality may offer refugees an alternative right to the city while allowing them to escape the gaze of the humanitarian-aid apparatus that can signify their vulnerability by reducing them to only being aid recipients. Instead, they form protective socio-spatial networks that have proved to be powerful in sustaining their livelihoods, guarding them from possible social discrimination or political threats

Structural and hydrogeological features of a Lias carbonate aquifer in the Triffa Plain, NE Morocco

The rising demand for water and the contamination of shallow water table aquifers has led authorities in NE Morocco to look for deeper groundwater resources in the Triffa Plain, namely in Lower Jurassic (Lias) dolomitic limestones. The liassic aquifer is of strategic importance for the development of the region, however, its hydrodynamic behaviour is poorly understood due to lack of hydrogeological data and block structure. This article presents a first effort towards understanding the structure and hydraulic behaviour of the aquifer. Exploration borehole data and results from geophysical campaigns were integrated into a GIS environment to build a preliminary model of the aquifer structure. The aquifer behaves as an unconfined aquifer in the northern part of the Béni Snassen Mountains (the recharge area), but as it dips to the north, it becomes confined by marls and shales of the Middle/Upper Jurassic. Even though piezometric level data are scarce, a tentative piezometric map was produced. Three blocks separated by NW–SE trending faults in a horst and graben structure, with distinct flow behaviours were identified: Berkane, Fezouane and Sidi Rahmoun blocks. Those blocks also show differences in hydraulic conductivity distribution. As a result of the reaction with the dolomitic limestones, the groundwater is of calcium-magnesium bicarbonate type. Groundwater temperature as measured in springs ranges from 29 C to 37 C in springs and constitutes a potential low enthalpy geothermal resource

Contemporary tendencies of the global foreign exchange market development

Метою кваліфікаційної роботи є визначення особливостей та проблем світового ринку FOREX, проаналізувати його сучасний стан. Для досягнення поставленої мети необхідно вирішити наступні завдання: - розглянути сутність, еволюцію та функції валютних ринків; - вивчити класифікацію валютних ринків; - визначити особливості діяльності учасників валютного ринку; - проаналізувати ринок форекс та його роль у світовій фінансовій системі; - визначити вплив криптовалют на валютний ринок.The aim of the qualification work is to identify the features and problems of the global FOREX market, to analyze its current state. To achieve this goal, it is necessary to solve the following tasks: - to consider the essence, evolution and functions of foreign exchange markets; - to study the classification of foreign exchange markets; - to determine the peculiarities of the activities of forex market participants; - to analyze the forex market and its role in the global financial system; - to determine the impact of cryptocurrencies on the forex market

Fully automated lung segmentation from chest radiographs using SLICO superpixels

This project aims to create a computer-aided diagnosis (CAD) system that can be used to identify tuberculosis (TB) from chest radiographs (CXRs) and, in particular, to observe the progress of the disease where patients have had multiple images over a period of time. Such a CAD tool, if sufficiently automated could run in the background checking every CXR taken, regardless of whether the patient is a suspected carrier of TB. This paper outlines the first phase of the project: segmenting the lung region from a CXR. This is a challenge because of the variation in the appearance of the lung in different patients and even in images of the same patient

A low noise amplifier suitable for biomedical recording analog front-end in 65nm CMOS technology

This paper presents a fully integrated Front-end, low noise amplifier, dedicated to the processing of various types of bio-medical signals, such as Electrocardiogram (ECG), Electroencephalography (EEG), Axon Action Potential (AAP). A novel noise reduction technique, for an operational transconductance amplifier (OTA), has been proposed. This adds a current steering branch parallel to the differential pair, with a view to reducing the noise contribution by the cascode current sources. Hence, this reduces the overall input referred noise of the Low Noise Amplifier (LNA), without adding any additional power. The proposed technique implemented in 65nm CMOS technology achieves 30dB closed loop voltage gain, 0.05Hz lower cut-off frequency and 100MHz 3-dB bandwidth. It operates at 1.2V power supply and draws 1µA static current. The prototype described in this paper occupies 3300µm2silicon area

Characterisation of pellicles formed by acinetobacter baumannii at the air-liquidi interface

The clinical importance of Acinetobacter baumannii is partly due to its natural ability to survive in the hospital environment. This persistence may be explained by its capacity to form biofilms and, interestingly, A. baumannii can form pellicles at the air-liquid interface more readily than other less pathogenic Acinetobacter species. Pellicles from twenty-six strains were morphologically classified into three groups: I) egg-shaped (27%); II) ball-shaped (50%); and III) irregular pellicles (23%). One strain representative of each group was further analysed by Brewster's Angle Microscopy to follow pellicle development, demonstrating that their formation did not require anchoring to a solid surface. Total carbohydrate analysis of the matrix showed three main components: Glucose, GlcNAc and Kdo. Dispersin B, an enzyme that hydrolyzes poly-N-acetylglucosamine (PNAG) polysaccharide, inhibited A. baumannii pellicle formation, suggesting that this exopolysaccharide contributes to pellicle formation. Also associated with the pellicle matrix were three subunits of pili assembled by chaperon-usher systems: the major CsuA/B, A1S_1510 (presented 45% of identity with the main pilin F17-A from enterotoxigenic Escherichia coli pili) and A1S_2091. The presence of both PNAG polysaccharide and pili systems in matrix of pellicles might contribute to the virulence of this emerging pathogen

COSMOS: Python library for massively parallel workflows

Summary: Efficient workflows to shepherd clinically generated genomic data through the multiple stages of a next-generation sequencing pipeline are of critical importance in translational biomedical science. Here we present COSMOS, a Python library for workflow management that allows formal description of pipelines and partitioning of jobs. In addition, it includes a user interface for tracking the progress of jobs, abstraction of the queuing system and fine-grained control over the workflow. Workflows can be created on traditional computing clusters as well as cloud-based services. Availability and implementation: Source code is available for academic non-commercial research purposes. Links to code and documentation are provided at http://lpm.hms.harvard.edu and http://wall-lab.stanford.edu. Contact: [email protected] or [email protected]. Supplementary information: Supplementary data are available at Bioinformatics online

TGF-ß Regulates Enamel Mineralization and Maturation through KLK4 Expression

Transforming growth factor-ß (TGF-ß) signaling plays an important role in regulating crucial biological processes such as cell proliferation, differentiation, apoptosis, and extracellular matrix remodeling. Many of these processes are also an integral part of amelogenesis. In order to delineate a precise role of TGF-ß signaling during amelogenesis, we developed a transgenic mouse line that harbors bovine amelogenin promoter-driven Cre recombinase, and bred this line with TGF-ß receptor II floxed mice to generate ameloblast-specific TGF-ß receptor II conditional knockout (cKO) mice. Histological analysis of the teeth at postnatal day 7 (P7) showed altered enamel matrix composition in the cKO mice as compared to the floxed mice that had enamel similar to the wild-type mice. The µCT and SEM analyses revealed decreased mineral content in the cKO enamel concomitant with increased attrition and thinner enamel crystallites. Although the mRNA levels remained unaltered, immunostaining revealed increased amelogenin, ameloblastin, and enamelin localization in the cKO enamel at the maturation stage. Interestingly, KLK4 mRNA levels were significantly reduced in the cKO teeth along with a slight increase in MMP-20 levels, suggesting that normal enamel maturation is regulated by TGF-ß signaling through the expression of KLK4. Thus, our study indicates that TGF-ß signaling plays an important role in ameloblast functions and enamel maturation