GSU Event Portal

Creating an event landing page or website is a very important step in your event marketing tactic as it acts as a hub for all things associated to your event. Because this is the best way to reach maximum number of people. With the help of social media nowadays there is huge impact of web portals in the market. For an any event, there is need to create an awareness among the people that particular event is going to happen. With this portal we can reach people very easily make them know what is the event all about and who can participate and a lot more information can be shared among them. This is not completely a new application, there are lot more event portals, this is just an alternative to the existing model/service. This is a Web Based system, the basic idea behind designing this application is that the user can plan and create the event according to his requirement, needs and budget. System very efficiently store, access and maintain data from database and can be used for further analysis. This project is a user interactive web application. The system will help the user to create an event. Our Event Management System is especially design to reduce the communication gap between event organizer and clients. Easy to manage historical data in database. User can select the theme for their event virtually on his computer. Participants can register for any happening event from anywhere. Event Organizer can keep records of participants. This project provides a platform to promote events by event organizers where customers can find nearby events using customer’s current location. It allows any user to create any event with the parameters are category, event type, date and price with landmarks. This project primarily focusing on creating and promoting an event for the event organizers. It helps users to find specific event based on category with the details of travel distance and price. It provides refine search facility to users to search This application featuring services are location identifiers with the help of longitude and latitude points, it provides social bar for each and every event to share with the friends and also supporting saving an event feature. for an event using location, category, date and price and also displays relevant events to the users. Modules: Admin Admin have all the access, he can add, update, delete any information in the system. He can add new event, event manager, Volunteer as well as update them. Admin also have the user credentials to enter into the system. He can able to see all the participants who are registered for an Event. Admin can read all the feedback given by visitors about events. User In this system users are event visitors and event creators. User have to register for event creation or event visit. Can easily check event details times and can contact event organizer easily. Participants can register online and able to get notification about event timing, place or any updates. User can give the feedback about the Event. He can search the events by location, date, event type, Free or paid. Organizer Organizer also has Credentials to login into his panel. He maintains the total no of visitors who are registered for a particular Event. He is responsible for all needs of an Event

Author Correction: Cancer Testis Antigen Promotes Triple Negative Breast Cancer Metastasis and is Traceable in the Circulating Extracellular Vesicles (Scientific Reports, (2019), 9, 1, (11632), 10.1038/s41598-019-48064-w)

Triple negative breast cancer (TNBC) has poor survival, exhibits rapid metastases, lacks targeted therapies and reliable prognostic markers. Here, we examined metastasis promoting role of cancer testis antigen SPANXB1 in TNBC and its utility as a therapeutic target and prognostic biomarker. Expression pattern of SPANXB1 was determined using matched primary cancer, lymph node metastatic tissues and circulating small extracellular vesicles (sEVs). cDNA microarray analysis of TNBC cells stably integrated with a metastasis suppressor SH3GL2 identified SPANXB1 as a potential target gene. TNBC cells overexpressing SH3GL2 exhibited decreased levels of both SPANXB1 mRNA and protein. Silencing of SPANXB1 reduced migration, invasion and reactive oxygen species production of TNBC cells. SPANXB1 depletion augmented SH3GL2 expression and decreased RAC-1, FAK, A-Actinin and Vinculin expression. Phenotypic and molecular changes were reversed upon SPANXB1 re-expression. SPANXB1 overexpressing breast cancer cells with an enhanced SPANXB1:SH3GL2 ratio achieved pulmonary metastasis within 5 weeks, whereas controls cells failed to do so. Altered expression of SPANXB1 was detected in the sEVs of SPANXB1 transduced cells. Exclusive expression of SPANXB1 was traceable in circulating sEVs, which was associated with TNBC progression. SPANXB1 represents a novel and ideal therapeutic target for blocking TNBC metastases due to its unique expression pattern and may function as an EV based prognostic marker to improve TNBC survival. Uniquely restricted expression of SPANXB1 in TNBCs, makes it an ideal candidate for targeted therapeutics and prognostication

Arpc1b, a centrosomal protein, is both an activator and substrate of Aurora A

In addition to its function as an Arp2/3 complex subunit, Arp1cb interacts with and stimulates Aurora A at centrosomes, functioning in cell cycle progression

Biocontrol of charcoal-rot of sorghum by actinomycetes isolated from herbal vermicompost

A total of 137 actinomycetes, isolated from 25 different herbal vermicomposts, were characterized for their antagonistic potential against Macrophomina phaseolina by dual-culture assay. Of them, eight most promising isolates (CAI-17, CAI-21, CAI-26, CAI-68, CAI-78, KAI-26, KAI-27 and MMA-32) were characterized for the production of siderophore, chitinase, protease, hydrocyanic acid (HCN), indole acetic acid (IAA) and further evaluated for their antagonistic potential against M. phaseolina by blotter-paper assay and in greenhouse. All the eight isolates produced HCN and IAA, seven produced siderophore (except CAI-78) and protease (except KAI-27) and four produced chitinase (CAI-26, KAI-26, KAI-27 and MMA-32). In the blotter-paper assay, no charcoal-rot infection was observed in KAI-26 and KAI-27-treated sorghum roots, indicating complete inhibition of the pathogen, while the other isolates showed 47 to 88% lesser charcoal-rot infection compared to the control. In the antifungal activity test against M. phaseolina (in greenhouse on sorghum), all the isolates increased in shoot dry mass by 28 to 53% and root dry mass by 5 to 21%, over the control. In order to confirm the plant growth promoting (PGP) traits of the isolates, the green house experiment was repeated, but in the absence of M. phaseolina. The results further confirmed the PGP traits of the isolates as evidenced by 15 to 34% increase in shoot dry mass on six isolates (except CAI-26 and KAI-27), 14 to 57% increase in root dry mass on five isolates (except CAI-68, KAI-26 and KAI-27), 17 to 60% increase in root length on five isolates (except CAI-17, CAI-21 and CAI-68) and 10 to 64% increase in root volume on six isolates (except CAI-17 and CAI-68). Culture filtrate of three potential actinomycetes (CAI-21, CAI-26 and MMA-32) at 0.5% inhibited the growth of M. phaseolina, indicating that the metabolites of these actinomycetes were responsible for the inhibition. The sequences of 16S rDNA gene of the isolates matched with Streptomyces but with different species in BLAST analysis. This study indicates that the selected actinomycetes have the potential for PGP and control of charcoal-rot disease in sorghum

p21-activated kinase signaling in breast cancer

The p21-activated kinases signal through a number of cellular pathways fundamental to growth, differentiation and apoptosis. A wealth of information has accumulated at an impressive pace in the recent past, both with regard to previously identified targets for p21-activated kinases that regulate the actin cytoskeleton and cellular stress pathways and with regard to newly identified targets and their role in cancer. Emerging data also provide new clues towards a previously unappreciated link between these various cellular processes. The present review attempts to provide a quick tutorial to the reader about the evolving significance of p21-activated kinases and small GTPases in breast cancer, using information from mouse models, tissue culture studies, and human materials

Lysophosphatidic Acid Induces MDA-MB-231 Breast Cancer Cells Migration through Activation of PI3K/PAK1/ERK Signaling

Enhanced motility of cancer cells is a critical step in promoting tumor metastasis. Lysophosphatidic acid (LPA), representing the major mitogenic activity in serum, stimulates migration in various types of cancer cells. However, the underlying signaling mechanisms for LPA-induced motility of cancer cells remain to be elucidated.In this study, we found that LPA dose-dependently stimulated migration of MDA-MB-231 breast cancer cells, with 10 µM being the most effective. LPA also increased ERK activity and the MEK inhibitor U0126 could block LPA-induced ERK activity and cell migration. In addition, LPA induced PAK1 activation while ERK activation and cell migration were inhibited by ectopic expression of an inactive mutant form of PAK1 in MDA-MB-231 cells. Furthermore, LPA increased PI3K activity, and the PI3K inhibitor LY294002 inhibited both LPA-induced PAK1/ERK activation and cell migration. Moreover, in the breast cancer cell, LPA treatment resulted in remarkable production of reactive oxygen species (ROS), while LPA-induced ROS generation, PI3K/PAK1/ERK activation and cell migration could be inhibited by N-acetyl-L-Cysteine, a scavenger of ROS.Taken together, this study identifies a PI3K/PAK1/ERK signaling pathway for LPA-stimulated breast cancer cell migration. These data also suggest that ROS generation plays an essential role in the activation of LPA-stimulated PI3K/PAK1/ERK signaling and breast cancer cell migration. These findings may provide a basis for designing future therapeutic strategy for blocking breast cancer metastasis

RhoD regulates cytoskeletal dynamics via the actin nucleation-promoting factor WASp homologue associated with actin Golgi membranes and microtubules

The Rho GTPases have mainly been studied in association with their roles in the regulation of actin filament organization. These studies have shown that the Rho GTPases are essential for basic cellular processes, such as cell migration, contraction, and division. In this paper, we report that RhoD has a role in the organization of actin dynamics that is distinct from the roles of the better-studied Rho members Cdc42, RhoA, and Rac1. We found that RhoD binds the actin nucleation–promoting factor WASp homologue associated with actin Golgi membranes and microtubules (WHAMM), as well as the related filamin A–binding protein FILIP1. Of these two RhoD-binding proteins, WHAMM was found to bind to the Arp2/3 complex, while FILIP1 bound filamin A. WHAMM was found to act downstream of RhoD in regulating cytoskeletal dynamics. In addition, cells treated with small interfering RNAs for RhoD and WHAMM showed increased cell attachment and decreased cell migration. These major effects on cytoskeletal dynamics indicate that RhoD and its effectors control vital cytoskeleton-driven cellular processes. In agreement with this notion, our data suggest that RhoD coordinates Arp2/3-dependent and FLNa-dependent mechanisms to control the actin filament system, cell adhesion, and cell migration

Specific induction of pp125 focal adhesion kinase in human breast cancer

The pp125 focal adhesion kinase (FAK) is involved in integrin-mediated cell signalling and overexpressed in a variety of solid tumours. Focal adhesion kinase expression has been correlated to invasion and metastasis, but the data on breast cancer are inconclusive. We analysed FAK mRNA, protein levels and expression patterns in primary breast cancer and normal breast tissue. FAK expression on the functional protein level and mRNA was determined in 55 matched pairs of breast cancer and corresponding normal tissue by Western blot, immunohistochemistry and RT–PCR. Using a score ranging from 0 to +5 for Western blots, we determined in normal breast tissue a score of 1.51±0.84 (mean±standard deviation), which was strongly induced to 2.91 (±1.22) in breast cancers (P<0.001). Overall, 45 out of 55 tissue pairs (81.8%) showed this upregulation of FAK protein in tumours in comparison to normal tissue. Immunohistochemistry confirmed these findings with a significant higher score for tumours vs physiological tissue (1.0±0.63 vs 2.27±0.91; P=0.001). Interestingly, no overall significant difference in the mRNA levels (P=0.359) was observed. In conclusion, expression levels of the FAK protein are specifically upregulated in breast cancer in comparison to matched normal breast tissue supporting its pivotal role in neoplastic signal transduction and representing a potential marker for malignant transformation