Building cloud applications for challenged networks

Cloud computing has seen vast advancements and uptake in many parts of the world. However, many of the design patterns and deployment models are not very suitable for locations with challenged networks such as countries with no nearby datacenters. This paper describes the problem and discusses the options available for such locations, focusing specifically on community clouds as a short-term solution. The paper highlights the impact of recent trends in the development of cloud applications and how changing these could better help deployment in challenged networks. The paper also outlines the consequent challenges in bridging different cloud deployments, also known as cross-cloud computing

Manufacturing Macroporous Monoliths of Microporous Metal-Organic Frameworks

We report the first use of a pressure-assisted sintering technique on metal-organic framework (MOF) powders allowing for fast production of crystalline macro-porous monoliths. Mechanical and micro-structural characterization of the monoliths is presented. The interconnected macro-porosity of the compacts can be adjusted providing an immense interface for gas-solid interaction. The sintering technique is well-established and commercially available, allowing industrial scale-up of the process

A Differential Effect of E. coli Toxin-Antitoxin Systems on Cell Death in Liquid Media and Biofilm Formation

Toxin-antitoxin (TA) modules are gene pairs specifying for a toxin and its antitoxin and are found on the chromosomes of many bacteria including pathogens. Here we report how each of five such TA systems in E. coli affect bacterial cell death differently in liquid media and during biofilm formation. Of all these systems, only the TA system mazEF mediated cell death both in liquid media and during biofilm formation. At the other extreme, as our results have revealed here, the TA system dinJ-YafQ is unique in that it is involved only in the death process during biofilm formation. Cell death governed by mazEF and dinJ-YafQ seems to participate in biofilm formation through a novel mechanism

Olfactory dysfunction: a diagnostic symptom of COVID-19

Besides the common respiratory symptoms or viral pneumonia, COVID-19 is also presented with different neurological symptoms. Olfactory dysfunction (OD) or impairment of the sense of smell is one of the common neurological symptoms being reported in infection caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). The severity varies from anosmia to microsmia or hyposmia. OD could have a potential early screening and diagnostic value besides other neurological and common respiratory symptoms. In our descriptive concise review, we aimed to elicit the manifestation of olfactory dysfunction as an early predictor of SARS-CoV-2 infection. We are also aiming to establish, OD as a quick and reliable assessment tool of COVID-19 risk, among the healthcare workers and contact tracers, which can justify self-quarantine of the person as well as recommendation for testing on a priority basis. We have also focused on, any effect to produce this symptom by the drug used for treatment in COVID-19, and if the old age of the patient showing any extra influence to develop OD in COVID-19

Escherichia coli MazF Leads to the Simultaneous Selective Synthesis of Both “Death Proteins” and “Survival Proteins”

The Escherichia coli mazEF module is one of the most thoroughly studied toxin–antitoxin systems. mazF encodes a stable toxin, MazF, and mazE encodes a labile antitoxin, MazE, which prevents the lethal effect of MazF. MazF is an endoribonuclease that leads to the inhibition of protein synthesis by cleaving mRNAs at ACA sequences. Here, using 2D-gels, we show that in E. coli, although MazF induction leads to the inhibition of the synthesis of most proteins, the synthesis of an exclusive group of proteins, mostly smaller than about 20 kDa, is still permitted. We identified some of those small proteins by mass spectrometry. By deleting the genes encoding those proteins from the E. coli chromosome, we showed that they were required for the death of most of the cellular population. Under the same experimental conditions, which induce mazEF-mediated cell death, other such proteins were found to be required for the survival of a small sub-population of cells. Thus, MazF appears to be a regulator that induces downstream pathways leading to death of most of the population and the continued survival of a small sub-population, which will likely become the nucleus of a new population when growth conditions become less stressful

Improved conductivity in dye-sensitised solar cells through block-copolymer confined TiO2 crystallisation

Anatase TiO2 is typically a central component in high performance dye-sensitised solar cells (DSCs). This study demonstrates the benefits of high temperature synthesised mesoporous titania for the performance of solid-state DSCs. In contrast to earlier methods, the high temperature stability of mesoporous titania is enabled by the self-assembly of the amphiphilic block copolymer polyisoprene-block-polyethylene oxide (PI-b -PEO) which compartmentalises TiO2 crystallisation, preventing the collapse of porosity at temperatures up to 700 degrees C. The systematic study of the temperature dependence on DSC performance reveals a parameter trade-off: high temperature annealed anatase consisted of larger crystallites and had a higher conductivity, but this came at the expense of a reduced specific surface area. While the reduction in specific surface areas was found to be detrimental for liquid-electrolyte DSC performance, solid-state DSCs benefitted from the increased anatase conductivity and exhibited a performance increase by a factor of three

A Toxin-Antitoxin Module in Bacillus subtilis Can Both Mitigate and Amplify Effects of Lethal Stress

Bacterial type-2 (protein-protein) toxin-antitoxin (TA) modules are two-gene operons that are thought to participate in the response to stress. Previous work with Escherichia coli has led to a debate in which some investigators conclude that the modules protect from stress, while others argue that they amplify lethal stress and lead to programmed cell death. To avoid ambiguity arising from the presence of multiple TA modules in E. coli, the effect of the sole type-2 toxin-antitoxin module of Bacillus subtilis was examined for several types of lethal stress.Genetic knockout of the toxin gene, ndoA (ydcE), conferred protection to lethal stressors that included kanamycin, moxifloxacin, hydrogen peroxide, and UV irradiation. However, at low doses of UV irradiation the ndoA deficiency increased lethality. Indeed, gradually increasing UV dose with the ndoA mutant revealed a crossover response--from the mutant being more sensitive than wild-type cells to being less sensitive. For high temperature and nutrient starvation, the toxin deficiency rendered cells hypersensitive. The ndoA deficiency also reduced sporulation frequency, indicating a role for toxin-antitoxin modules in this developmental process. In the case of lethal antimicrobial treatment, deletion of the toxin eliminated a surge in hydrogen peroxide accumulation observed in wild-type cells.A single toxin-antitoxin module can mediate two opposing effects of stress, one that lowers lethality and another that raises it. Protective effects are thought to arise from toxin-mediated inhibition of translation based on published work. The enhanced, stress-mediated killing probably involves toxin-dependent accumulation of reactive oxygen species, since a deficiency in the NdoA toxin suppressed peroxide accumulation following antimicrobial treatment. The type and perhaps the level of stress appear to be important for determining whether this toxin will have a protective or detrimental effect

The Indian COSMOS Network (ICON): validating L-band remote sensing and modelled soil moisture data products

Availability of global satellite based Soil Moisture (SM) data has promoted the emergence of many applications in climate studies, agricultural water resource management and hydrology. In this context, validation of the global data set is of substance. Remote sensing measurements which are representative of an area covering 100 m2 to tens of km2 rarely match with in situ SM measurements at point scale due to scale difference. In this paper we present the new Indian Cosmic Ray Network (ICON) and compare it’s data with remotely sensed SM at different depths. ICON is the first network in India of the kind. It is operational since 2016 and consist of seven sites equipped with the COSMOS instrument. This instrument is based on the Cosmic Ray Neutron Probe (CRNP) technique which uses non-invasive neutron counts as a measure of soil moisture. It provides in situ measurements over an area with a radius of 150–250 m. This intermediate scale soil moisture is of interest for the validation of satellite SM. We compare the COSMOS derived soil moisture to surface soil moisture (SSM) and root zone soil moisture (RZSM) derived from SMOS, SMAP and GLDAS_Noah. The comparison with surface soil moisture products yield that the SMAP_L4_SSM showed best performance over all the sites with correlation (R) values ranging from 0.76 to 0.90. RZSM on the other hand from all products showed lesser performances. RZSM for GLDAS and SMAP_L4 products show that the results are better for the top layer R = 0.75 to 0.89 and 0.75 to 0.90 respectively than the deeper layers R = 0.26 to 0.92 and 0.6 to 0.8 respectively in all sites in India. The ICON network will be a useful tool for the calibration and validation activities for future SM missions like the NASA-ISRO Synthetic Aperture Radar (NISAR)

Role of RecA and the SOS Response in Thymineless Death in Escherichia coli

Thymineless death (TLD) is a classic and enigmatic phenomenon, documented in bacterial, yeast, and human cells, whereby cells lose viability rapidly when deprived of thymine. Despite its being the essential mode of action of important chemotherapeutic agents, and despite having been studied extensively for decades, the basic mechanisms of TLD have remained elusive. In Escherichia coli, several proteins involved in homologous recombination (HR) are required for TLD, however, surprisingly, RecA, the central HR protein and activator of the SOS DNA–damage response was reported not to be. We demonstrate that RecA and the SOS response are required for a substantial fraction of TLD. We show that some of the Rec proteins implicated previously promote TLD via facilitating activation of the SOS response and that, of the roughly 40 proteins upregulated by SOS, SulA, an SOS–inducible inhibitor of cell division, accounts for most or all of how SOS causes TLD. The data imply that much of TLD results from an irreversible cell-cycle checkpoint due to blocked cell division. FISH analyses of the DNA in cells undergoing TLD reveal blocked replication and apparent DNA loss with the region near the replication origin underrepresented initially and the region near the terminus lost later. Models implicating formation of single-strand DNA at blocked replication forks, a SulA-blocked cell cycle, and RecQ/RecJ-catalyzed DNA degradation and HR are discussed. The data predict the importance of DNA damage-response and HR networks to TLD and chemotherapy resistance in humans