The Gulf of Cádiz: thrusting or strike-slip motion?

In the Gulf of Cádiz (SW Iberian Peninsula) the boundary between Eurasia and Nubia plates corresponds to a narrow band well defined by the seismicity, where large earthquakes (M>7.0) may be associated to N-S to NNW-SSE horizontal compression due to the convergence between the two plates. Most of these earthquakes are at shallow depth (less than 40 km), with some foci at intermediate-depth, showing E-W distribution and limited by a narrow band less than 20 km wide that broadens as we move to the Strait of Gibraltar. In this area the lithospheric material is relatively rigid and the stresses are released by larger earthquakes. General tectonic models proposed for the Azores-Tunisia plate boundary explain fairly well the nature of its seismicity and tectonic motions; however, details of some of its aspects are still poorly understood and controversial. Zittelini et al. (2009) has recently proposed for the Gulf of Cádiz, transcurrent-transpressional motion along a long strike slip fault based on multichannel seismic reflection surveys. However, this contradicts the compressional motion and reverse faulting of large 1755 Lisbon earthquake (~Mw9), which generate a large tsunami and the recent moderate (Mw>6.0) in 1964, 2007 and 2009 and large (Mw= 8.0) in 1969 earthquakes occurred in the region. These earthquakes show thrusting motion along E-W faults with the southern block going under, corresponding to horizontal NW-SE compression, and they can be related directly to the plate convergence between Nubia and Iberia. References Zitellini et al., 2009. The quest for the Africa–Eurasia plate boundary west of the Strait of Gibraltar. Earth and Planetary Science Letters 280 (2009) 13–50

Geoarcheologicalprospection of megalithic structures using Ground Penetrating Radar (Almendralejo, Spain)

In 1980, in Almendralejo, Spain, it was discovered the Tomb of Huerta Montero, a megalithic funerary monument, the best preserved in the Iberian Peninsula, dating from the Chalcolithic (Copper Age, about 4650 BC). According to the opinion of archaeologists, it is quite probable that there are similar buried monuments in the vicinity of the known tomb. This suspicion led to the geophysical study, carried out with the aim of identifying new structures in the vicinity of the necropolis discovered. The choice of geophysical method it was the GPR, because it is a non-invasive technique, accurate and rapid in its implementation. The results obtained allow us to conclude that there may be a structure of circular shape, with a diameter of about 7 m which may to correspond to a funeral monument as there are a few meters, with a temporal thickness ranging between 13 and 21 ns deep. In the northern part of the structure there are two protrusions that may match an entry in the building or even the rest of the tunnel entrance

Learning from Unlabelled data with Transformers: Domain Adaptation for Semantic Segmentation of High Resolution Aerial Images

Data from satellites or aerial vehicles are most of the times unlabelled. Annotating such data accurately is difficult, requires expertise, and is costly in terms of time. Even if Earth Observation (EO) data were correctly labelled, labels might change over time. Learning from unlabelled data within a semi-supervised learning framework for segmentation of aerial images is challenging. In this paper, we develop a new model for semantic segmentation of unlabelled images, the Non-annotated Earth Observation Semantic Segmentation (NEOS) model. NEOS performs domain adaptation as the target domain does not have ground truth masks. The distribution inconsistencies between the target and source domains are due to differences in acquisition scenes, environment conditions, sensors, and times. Our model aligns the learned representations of the different domains to make them coincide. The evaluation results show that it is successful and outperforms other models for semantic segmentation of unlabelled data

Consensus on circulatory shock and hemodynamic monitoring. Task force of the European Society of Intensive Care Medicine.

OBJECTIVE: Circulatory shock is a life-threatening syndrome resulting in multiorgan failure and a high mortality rate. The aim of this consensus is to provide support to the bedside clinician regarding the diagnosis, management and monitoring of shock. METHODS: The European Society of Intensive Care Medicine invited 12 experts to form a Task Force to update a previous consensus (Antonelli et al.: Intensive Care Med 33:575-590, 2007). The same five questions addressed in the earlier consensus were used as the outline for the literature search and review, with the aim of the Task Force to produce statements based on the available literature and evidence. These questions were: (1) What are the epidemiologic and pathophysiologic features of shock in the intensive care unit ? (2) Should we monitor preload and fluid responsiveness in shock ? (3) How and when should we monitor stroke volume or cardiac output in shock ? (4) What markers of the regional and microcirculation can be monitored, and how can cellular function be assessed in shock ? (5) What is the evidence for using hemodynamic monitoring to direct therapy in shock ? Four types of statements were used: definition, recommendation, best practice and statement of fact. RESULTS: Forty-four statements were made. The main new statements include: (1) statements on individualizing blood pressure targets; (2) statements on the assessment and prediction of fluid responsiveness; (3) statements on the use of echocardiography and hemodynamic monitoring. CONCLUSIONS: This consensus provides 44 statements that can be used at the bedside to diagnose, treat and monitor patients with shock

Evaluation and optimization of PCR primers for selective and quantitative detection of marine ANME subclusters involved in sulfate-dependent anaerobic methane oxidation

Since the discovery that anaerobic methanotrophic archaea (ANME) are involved in the anaerobic oxidation of methane coupled to sulfate reduction in marine sediments, different primers and probes specifically targeting the 16S rRNA gene of these archaea have been developed. Microbial investigation of the different ANME subtypes (ANME-1; ANME-2a, b, and c; and ANME-3) was mainly done in sediments where specific subtypes of ANME were highly enriched and methanogenic cell numbers were low. In different sediments with higher archaeal diversity and abundance, it is important that primers and probes targeting different ANME subtypes are very specific and do not detect other ANME subtypes or methanogens that are also present. In this study, primers and probes that were regularly used in AOM studies were tested in silico on coverage and specificity. Most of the previously developed primers and probes were not specific for the ANME subtypes, thereby not reflecting the actual ANME population in complex samples. Selected primers that showed good coverage and high specificity for the subclades ANME-1, ANME-2a/b, and ANME-2c were thoroughly validated using quantitative polymerase chain reaction (qPCR). From these qPCR tests, only certain combinations seemed suitable for selective amplification. After optimization of these primer sets, we obtained valid primer combinations for the selective detection and quantification of ANME-1, ANME-2a/b, and ANME-2c in samples where different ANME subtypes and possibly methanogens could be present. As a result of this work, we propose a standard workflow to facilitate selection of suitable primers for qPCR experiments on novel environmental samples.This research is supported by the Dutch Technology Foundation STW (project 10711), which is part of the Netherlands Organization for Scientific Research (NWO), and which is partly funded by the Ministry of Economic Affairs. Research of AJMS is supported by ERC grant (project 323009). Research of PHATand AJMS is supported by the SIAM Gravitation grant (project 024.002.002) of the Netherlands Ministry of Education, Culture and Science and the Netherlands Science Foundation (NWO).info:eu-repo/semantics/publishedVersio

Exploring the Consistency of Data Collected in Archaeological Geophysics: A Case Study from the Iron Age Hillfort of Villasviejas del Tamuja (Extremadura, Spain)

Different geophysical methods applied at the settlement of Villasviejas del Tamuja (Botija, Spain) have identified robust anomalies located at the same position, but some anomalies are reflected by only one method. Furthermore, analysing the spatial correlation of these anomalies is of fundamental importance for obtaining a correct archaeological interpretation. In this work, we analysed the main results of electrical resistivity tomography (ERT), ground-penetrating radar (GPR) and magnetic gradiometry methods in a particular area of the archaeological site. In this analysis, we performed graphical and numerical spatial correlation analyses of the anomalies and observed strong agreement among the results provided by each method. Certain anomalies were reflected only in the magnetic and ERT studies. The results highlight the importance of applying several geophysical methods and performing spatial correlational analyses. Furthermore, the methodology that we have applied to evaluate the spatial correlation offers interesting results

Selective activation of striatal indirect pathway suppresses levodopa induced-dyskinesias

Levodopa (L-DOPA) administration remains the gold standard therapy for Parkinson's disease (PD). Despite several pharmacological advances in the use of L-DOPA, a high proportion of chronically treated patients continues to suffer disabling involuntary movements, namely, L-DOPA-induced dyskinesias (LIDs). As part of the effort to stop these unwanted side effects, the present study used a rodent model to identify and manipulate the striatal outflow circuitry responsible for LIDs. To do so, optogenetic technology was used to activate separately the striatal direct (D1R- expressing) and indirect (D2R- expressing) pathways in a mouse model of PD. Firstly, D1-cre or A2a-cre animals received unilateral injections of neurotoxin 6-hydroxydopamine (6-OHDA) to simulate the loss of dopamine observed in PD patients. The effects of independently stimulating each pathway were tested to see if experimental dyskinesias could be induced. Secondly, dopamine depleted A2a-cre animals received systemic L-DOPA to evoke dyskinetic movements. The ability of indirect pathway optogenetic stimulation to suppress pre-established LIDs was then tested. Selective manipulation of direct pathway evoked optodyskinesias both in dopamine depleted and intact animals, but optical inhibition of these neurons failed to suppress LIDs. On the other hand, selective activation of indirect striatal projection neurons produced an immediate and reliable suppression of LIDs. Thus, a functional dissociation has been found here whereby activation of D1R- and D2R-expressing projection neurons evokes and inhibits LIDs respectively, supporting the notion of tight interaction between the two striatal efferent systems in both normal and pathological conditions. This points to the importance of maintaining an equilibrium in the activity of both striatal pathways to produce normal movement. Finally, the ability of selective indirect pathway optogenetic activation to block the expression of LIDs in an animal model of PD sheds light on intrinsic mechanisms responsible for striatal-based dyskinesias and identifies a potential therapeutic target for suppressing LIDs in PD patients.This work was supported by: Marie Slodowksa-Curie Fellowship, H2020-MSCA-IF-2014_RI:660964-ROSNPD (LFH); and by grants from the Spanish Government: SAF2015–67239-P (JAO), BES-2016-077493 (ICM); CIBERNED (JAO, LFH). Fundación Gangoiti (YVW). Funding agencies: Marie Slodowksa-Curie Fellowship, H2020-MSCA-IF-2014_RI:660964-ROSNPD (LFH); and by grants from the Spanish Government: SAF2015–67239-P (JAO), BES-2016-077493 (ICM); CIBERNED (JAO, LFH). Fundación Gangoiti (YVW) and by grants PID2019-111693RB-100 funded by MICIN /AEI/http://dx.doi.org/10.13039/501100011033, by European Union's Horizon 2020 research and innovation program, AND-PD (grant agreement no. 84800), and by NextGenerationEU/PRTR (MICIN/CSIC/PTI+ NeuroAging) to R.M