Étude de la diversité des populations historiques de Montréal et de Québec par l’analyse de la morphologie dentaire : le cimetière catholique de la première église Notre-Dame (1691-1796) et le cimetière protestant Saint-Matthew de Québec (1771-1860)

Deux principaux événements colonisateurs ont apporté de nouvelles vagues de migration au Québec : La fondation de la Nouvelle France, de 1608 à 1763 et la conquête du territoire par les Britanniques après 1763. Afin d’étudier les différences et similarités entre ces dernières et les interactions possibles entre les migrants et les communautés locales déjà présentes sur le territoire, la morphologie dentaire, un outil permettant de proposer des interprétations d’ordre paléogénétique sur l’origine des populations passées, a été analysée pour les deux groupes suivants: 37 individus provenant du cimetière de la première église Notre-Dame à Montréal (1691-1796); et 61 individus provenant du cimetière de Saint-Matthew à Québec (1771-1860). À cette fin, le protocole de l’Arizona State University -Dental Anthropology System a été utilisé pour la collecte de données. La mesure moyenne de divergence et une analyse d’hétérogénéité des populations (Matrice R et Fst modifiés pour les données non-métriques) ont été ensuite calculées. Les valeurs de biodistance confirment que la majorité des individus observés pour les deux collections sont d’ascendance européenne. L’analyse intra-populationnelle a aussi permis d’identifier certains individus, probablement métis, qui s’approchent de la variation amérindienne. Il semble aussi, selon la matrice R et les valeurs Fst calculées pour les deux échantillons, que Notre-Dame est légèrement plus hétérogène et semble avoir incorporé une composante amérindienne un peu plus importante que Saint-Matthew, probablement par métissage, faisant suite, par exemple, à l’incorporation d’individus Amérindiens convertis dans les premières sociétés coloniales. Bien que nos résultats soient très préliminaires, la relation qu’ont entretenue ces deux populations d’origine européenne avec les populations locales, semble avoir varié au cours du temps, en fonction du contexte politique et économique des différentes vagues de migration européenne. Le degré de métissage plus élevé à Montréal au XVIIIe siècle qu’à Québec au XIXe siècle pourrait ainsi refléter un besoin plus pressant de la part des premiers migrants européens de se faire des alliés amérindiens en vue de la réussite du projet colonisateur.Two colonisation events occurred in Quebec, from 1608 to 1763 (New France), and after 1763 (British Regime), providing new waves of immigrants. In order to examine differences and similarities between the latter waves and the possible interactions between the immigrants and the local communities already living on the territory, dental morphology, which allows us to propose paleogenetic interpretations on the ancestry of past populations, has been analysed for the following two groups: 37 individuals from the cemetery of the Première Église Notre-Dame in Montreal (1691-1796); and 61 individuals from the cemetery of Saint-Matthew in Quebec City (1771-1860). We used the Arizona State University’s -Dental Anthropology System protocol for the observation of dental traits. Mean measures of divergence and population heterogeneity analysis (R Matrix and Fst modified for non-metric data) were calculated. Biodistance values confirm that the majority of the analysed individuals from both collections were of European ancestry. However, intra-population analysis was able to identify certain individuals who were closer to Native American variation. Furthermore, results of R matrix and Fst tests showed that Notre-Dame sample was slightly more heterogeneous. It seemed to have incorporated more of a Native American component than Saint-Matthew, probably through admixture, which was a consequence of the assimilation of “Christianised” Native Americans within the early colonial society. Therefore, although our results are preliminary, interactions between Europeans and local groups seem to have changed through time as a result of colonisation. The higher levels of admixture in the 18th century Montreal (in comparison to the 19th century Quebec City) might reflect a rather urgent need from the first European migrants to set up alliances with Native Americans for the long-term viability of the colony

Hydrogen trapping by VC precipitates and structural defects in a high strength Fe-Mn-C steel studied by small-angle neutron scattering

The trapping of hydrogen by VC precipitates and structural defects in high strength Fe-Mn-C steel was studied by small angle neutron scattering. No interaction between H and V in solid solution has been detected but a significant interaction between H and structural defects introduced by plastic deformation has been measured. This last effect was reversible upon outgassing of the H. Moreover a significant interaction between H and VC precipitates has been measured; 5 ppm wt. of H could be trapped in the precipitates. This is consistent with the homogeneous trapping of H within the precipitates rather than at the precipitate/matrix interface

Nonverbal expression of empathy in healthy human populations : a scoping review protocol

The present scoping review aims to understand the extent and type of evidence related to the nonverbal expression of empathy (and empathic concern) in healthy human (or human-like) empathizers across contexts

Direct interpretation of the X-ray and neutron three-dimensional difference pair distribution functions (3D-ΔPDFs) of yttria-stabilized zirconia

Three-dimensional difference pair distribution functions (3D-ΔPDFs) from X-ray and neutron diffraction experiments are reported for yttria-stabilized zirconia (Zr0.82Y0.18O1.91). A quantitative analysis of the signatures in the three-dimensional difference pair distribution functions is used to establish that oxygen ions neighbouring a vacancy shift by 0.525 (5) Å along ⟨1, 0, 0⟩ towards the vacancy while metal ions neighbouring a vacancy shift by 0.465 (2) Å along ⟨1, 1, 1⟩ away from the vacancy. The neutron 3D-ΔPDF shows a tendency for vacancies to cluster along ⟨½, ½, ½⟩, which results in sixfold coordinated metal ions

Learning-Based Dequantization For Image Restoration Against Extremely Poor Illumination

All existing image enhancement methods, such as HDR tone mapping, cannot recover A/D quantization losses due to insufficient or excessive lighting, (underflow and overflow problems). The loss of image details due to A/D quantization is complete and it cannot be recovered by traditional image processing methods, but the modern data-driven machine learning approach offers a much needed cure to the problem. In this work we propose a novel approach to restore and enhance images acquired in low and uneven lighting. First, the ill illumination is algorithmically compensated by emulating the effects of artificial supplementary lighting. Then a DCNN trained using only synthetic data recovers the missing detail caused by quantization

New insights on the mechanism of quinoline-based DNA methyltransferase inhibitors

Among the epigenetic marks, DNA methylation is one of the most studied. It is highly deregulated in numerous diseases, including cancer. Indeed, it has been shown that hypermethylation of tumor suppressor genes promoters is a common feature of cancer cells. Because DNA methylation is reversible, the DNA methyltransferases (DNMTs), responsible for this epigenetic mark, are considered promising therapeutic targets. Several molecules have been identified as DNMT inhibitors and, among the non-nucleoside inhibitors, 4-aminoquinoline-based inhibitors, such as SGI-1027 and its analogs, showed potent inhibitory activity. Here we characterized the in vitro mechanism of action of SGI-1027 and two analogs. Enzymatic competition studies with the DNA substrate and the methyl donor cofactor, S-adenosyl-L-methionine (AdoMet), displayed AdoMet non-competitive and DNA competitive behavior. In addition, deviations from the Michaelis-Menten model in DNA competition experiments suggested an interaction with DNA. Thus their ability to interact with DNA was established; although SGI-1027 was a weak DNA ligand, analog 5, the most potent inhibitor, strongly interacted with DNA. Finally, as 5 interacted with DNMT only when the DNA duplex was present, we hypothesize that this class of chemical compounds inhibit DNMTs by interacting with the DNA substrate

Multi-objective optimization as a tool to identify possibilities for future agricultural landscapes

Agricultural landscapes provide many functions simultaneously including food production, regulation of water and regulation of greenhouse gases. Thus, it is challenging to make land management decisions, particularly transformative changes, that improve on one function without unintended consequences on other functions. To make informed decisions the trade-offs between different landscape functions must be considered. Here, we use a multi-objective optimization algorithm with a model of crop production that also simulates environmental effects such as nitrous oxide emissions to identify trade-off frontiers and associated possibilities for agricultural management. Trade-offs are identified in three soil types, using wheat production in the UK as an example, then the trade-off for combined management of the three soils is considered. The optimisation algorithm identifies trade-offs between different objectives and allows them to be visualised. For example, we observed a highly non-linear trade-off between wheat yield and nitrous oxide emissions, illustrating where small changes might have a large impact. We used a cluster analysis to identify distinct management strategies with similar management actions and use these clusters to link the trade-off curves to possibilities for management. There were more possible strategies for achieving desirable environmental outcomes and remaining profitable when the management of different soil types was considered together. Interestingly, it was on the soil capable of the highest potential profit that lower profit strategies were identified as useful for combined management. Meanwhile, to maintain average profitability across the soils, it was necessary to maximise the profit from the soil with the lowest potential profit. These results are somewhat counterintuitive and so the range of strategies supplied by the model could be used to stimulate discussion amongst stakeholders. In particular, as some key objectives can be met in different ways, stakeholders could discuss the impact of these management strategies on other objectives not quantified by the model

Uterine Nodal expression supports maternal immunotolerance and establishment of the FOXP3+ regulatory T cell population during the preimplantation period

Pregnancy success is dependent on the establishment of maternal tolerance during the preimplantation period. The immunosuppressive function of regulatory T cells is critical to limit inflammation arising from implantation of the semi-allogeneic blastocyst. Insufficient maternal immune adaptations to pregnancy have been frequently associated with cases of female infertility and recurrent implantation failure. The role of Nodal, a secreted morphogen of the TGFβ superfamily, was recently implicated during murine pregnancy as its conditional deletion (NodalΔ/Δ) in the female reproductive tract resulted in severe subfertility. Here, it was determined that despite normal preimplantation processes and healthy, viable embryos, NodalΔ/Δ females had a 50% implantation failure rate compared to NodalloxP/loxP controls. Prior to implantation, the expression of inflammatory cytokines MCP-1, G-CSF, IFN-γ and IL-10 was dysregulated in the NodalΔ/Δ uterus. Further analysis of the preimplantation leukocyte populations in NodalΔ/Δ uteri showed an overabundance of infiltrating, pro-inflammatory CD11bhigh Ly6C+ macrophages coupled with the absence of CD4+ FOXP3+ regulatory T cells. Therefore, it is proposed that uterine Nodal expression during the preimplantation period has a novel role in the establishment of maternal immunotolerance, and its dysregulation should be considered as a potential contributor to cases of female infertility and recurrent implantation failure

Early T Cell Signalling Is Reversibly Altered in PD-1+ T Lymphocytes Infiltrating Human Tumors

To improve cancer immunotherapy, a better understanding of the weak efficiency of tumor-infiltrating T lymphocytes (TIL) is necessary. We have analyzed the functional state of human TIL immediately after resection of three types of tumors (NSCLC, melanoma and RCC). Several signalling pathways (calcium, phosphorylation of ERK and Akt) and cytokine secretion are affected to different extents in TIL, and show a partial spontaneous recovery within a few hours in culture. The global result is an anergy that is quite distinct from clonal anergy induced in vitro, and closer to adaptive tolerance in mice. PD-1 (programmed death -1) is systematically expressed by TIL and may contribute to their anergy by its mere expression, and not only when it interacts with its ligands PD-L1 or PD-L2, which are not expressed by every tumor. Indeed, the TCR-induced calcium and ERK responses were reduced in peripheral blood T cells transfected with PD-1. Inhibition by sodium stibogluconate of the SHP-1 and SHP-2 phosphatases that associate with several inhibitory receptors including PD-1, relieves part of the anergy apparent in TIL or in PD-1-transfected T cells. This work highlights some of the molecular modifications contributing to functional defects of human TIL