Updated CPT-based p–y formulation for laterally loaded piles in cohesionless soil under static loading

The p–y method is currently the most popular design method to predict the response of piles to lateral load. The authors had previously used numerical methods to develop a cone penetration test (CPT)-based p–y formulation for piles in sand and this has subsequently been shown by independent verification to show considerable promise. This paper addresses some of the uncertainties associated with the original p–y formulation by examining the influence of pile bending stiffness, the presence of a water table, the cross-sectional shape of the pile and soil non-homogeneities. Numerical experiments are presented examining these four effects and lead to an updated proposal for a CPT-based p–y formulation. This formulation, which is consistent with the original proposal, is validated against three-dimensional finite-element calculations and data obtained from a full-scale offshore monopile foundation supporting a wind turbine

Numerical derivation of CPT-based p-y curves for piles in sand

The formulations for the lateral load-displacement (p-y) springs conventionally used for the analysis of laterally loaded piles have been based largely on the back-analysis of the performance of small-scale instrumented piles subjected to lateral load. Although such formulations have been employed with much success in industry, their applicability to large-diameter piles, such as those used to support offshore wind turbines, is uncertain and has necessitated further research in this area. Moreover, with the growth in popularity of in-situ cone penetration tests (CPTs), there are demands for a theoretically supported direct method that can enable the derivation of p-y curves from the CPT end resistance (q c). In this paper, a numerical derivation of CPT-based p-y curves applicable to both small- and large-diameter laterally loaded single piles in sand is presented. Three-dimensional finite-element analyses are performed using a non-linear elasto-plastic soil model to predict the response of single piles in sand subjected to lateral loads. The corresponding CPT q c profile is derived using the same soil constitutive model by way of the cavity expansion analogue. An extensive series of computations of the lateral pile response and CPT q c values is then employed to formulate a direct method of constructing p-y curves from CPT q c values. The proposed method is shown to be generally consistent with existing empirical correlations and to provide good predictions in relation to the measurements obtained during lateral load tests on instrumented piles in an independent case study

The Response of Driven Single Piles Subjected to Combined Loads

The behaviour of piles subjected to lateral load has generally been investigated experimentally using free-headed piles with a lateral load applied close to the pile head. In practice, however, there is some degree of restraint at the head of many piles and these are often subjected to a combination of lateral and vertical loads. The case history described in this paper involved full-scale field experiments comprising instrumented precast concrete piles subjected to a range of loading conditions including combined lateral and axial loading with partial rotational restraint at the pile head. The pile instrumentation, which included electrolevels and electrical resistance strain gauges, allowed accurate determination of the lateral soil reaction-lateral displacement (p-y) response adjacent to the test piles. This paper concentrates on the analysis and interpretation of the test data for the pile subjected to combined loading. These results are presented in conjunction with test data from an adjacent pile subjected to the same lateral load to allow the difference in behaviour to be evaluated

Adult midgut expressed sequence tags from the tsetse fly Glossina morsitans morsitans and expression analysis of putative immune response genes

BACKGROUND: Tsetse flies transmit African trypanosomiasis leading to half a million cases annually. Trypanosomiasis in animals (nagana) remains a massive brake on African agricultural development. While trypanosome biology is widely studied, knowledge of tsetse flies is very limited, particularly at the molecular level. This is a serious impediment to investigations of tsetse-trypanosome interactions. We have undertaken an expressed sequence tag (EST) project on the adult tsetse midgut, the major organ system for establishment and early development of trypanosomes. RESULTS: A total of 21,427 ESTs were produced from the midgut of adult Glossina morsitans morsitans and grouped into 8,876 clusters or singletons potentially representing unique genes. Putative functions were ascribed to 4,035 of these by homology. Of these, a remarkable 3,884 had their most significant matches in the Drosophila protein database. We selected 68 genes with putative immune-related functions, macroarrayed them and determined their expression profiles following bacterial or trypanosome challenge. In both infections many genes are downregulated, suggesting a malaise response in the midgut. Trypanosome and bacterial challenge result in upregulation of different genes, suggesting that different recognition pathways are involved in the two responses. The most notable block of genes upregulated in response to trypanosome challenge are a series of Toll and Imd genes and a series of genes involved in oxidative stress responses. CONCLUSIONS: The project increases the number of known Glossina genes by two orders of magnitude. Identification of putative immunity genes and their preliminary characterization provides a resource for the experimental dissection of tsetse-trypanosome interactions

Verification of numerically derived CPT based p-y curves for piles in sand

This paper examines the potential of a numerically derived CPT-based formulation for p-y curves in sand (Suryasentana & Lehane 2014) to predict the response observed in lateral load tests conducted on six piles in four different sand deposits. A summary of the methodology employed in the derivation of this formulation is first described before presenting information related to each of the case histories examined. The lateral load displacement data measured in these case histories are shown to compare well with predictions obtained using the p-y formulation. This agreement should encourage further refinement of the formulation and ultimately the direct use of CPT qc profiles for the analysis of laterally loaded piles in sand

Bearing Capacity and Load-Displacement Behavior of Rigid Pads on Soft, Sensitive, Clay

The paper describes tests on rigid square pads at the UK national soft clay research site at Bothkennar, Scotland. The work was performed as a low cost adjunct to the instrumented pile research described by Lehane and Jardine (1992). Its aims were to investigate (i} bearing capacity, (ii) load-displacement response to short and long term loading, (iii) the applicability of relevant theories and (iv) relationships between soil properties determined in-situ and those measured in high quality laboratory tests

Probabilistic soil strata delineation using DPT data and Bayesian changepoint detection

Soil strata delineation is a fundamental step for any geotechnical engineering design. The dynamic penetration test (DPT) is a fast, low cost in situ test that is commonly used to locate boundaries between strata of differing density and driving resistance. However, DPT data are often noisy and typically require time-consuming, manual interpretation. This paper investigates a probabilistic method that enables delineation of dissimilar soil strata (where each stratum is deemed to belong to different soil groups based on their particle size distribution) by processing DPT data with Bayesian changepoint detection methods. The accuracy of the proposed method is evaluated using DPT data from a real-world case study, which highlights the potential of the proposed method. This study provides a methodology for faster DPT-based soil strata delineation, which paves the way for more cost-effective geotechnical designs

Vegetation and the importance of insecticide-treated target siting for control of Glossina fuscipes fuscipes

Control of tsetse flies using insecticide-treated targets is often hampered by vegetation re-growth and encroachment which obscures a target and renders it less effective. Potentially this is of particular concern for the newly developed small targets (0.25 high × 0.5 m wide) which show promise for cost-efficient control of Palpalis group tsetse flies. Consequently the performance of a small target was investigated for Glossina fuscipes fuscipes in Kenya, when the target was obscured following the placement of vegetation to simulate various degrees of natural bush encroachment. Catches decreased significantly only when the target was obscured by more than 80%. Even if a small target is underneath a very low overhanging bush (0.5 m above ground), the numbers of G. f. fuscipes decreased by only about 30% compared to a target in the open. We show that the efficiency of the small targets, even in small (1 m diameter) clearings, is largely uncompromised by vegetation re-growth because G. f. fuscipes readily enter between and under vegetation. The essential characteristic is that there should be some openings between vegetation. This implies that for this important vector of HAT, and possibly other Palpalis group flies, a smaller initial clearance zone around targets can be made and longer interval between site maintenance visits is possible both of which will result in cost savings for large scale operations. We also investigated and discuss other site features e.g. large solid objects and position in relation to the water's edge in terms of the efficacy of the small targets

An insight into the sialome of Glossina morsitans morsitans

<p>Abstract</p> <p>Background</p> <p>Blood feeding evolved independently in worms, arthropods and mammals. Among the adaptations to this peculiar diet, these animals developed an armament of salivary molecules that disarm their host's anti-bleeding defenses (hemostasis), inflammatory and immune reactions. Recent sialotranscriptome analyses (from the Greek <it>sialo </it>= saliva) of blood feeding insects and ticks have revealed that the saliva contains hundreds of polypeptides, many unique to their genus or family. Adult tsetse flies feed exclusively on vertebrate blood and are important vectors of human and animal diseases. Thus far, only limited information exists regarding the Glossina sialome, or any other fly belonging to the Hippoboscidae.</p> <p>Results</p> <p>As part of the effort to sequence the genome of <it>Glossina morsitans morsitans</it>, several organ specific, high quality normalized cDNA libraries have been constructed, from which over 20,000 ESTs from an adult salivary gland library were sequenced. These ESTs have been assembled using previously described ESTs from the fat body and midgut libraries of the same fly, thus totaling 62,251 ESTs, which have been assembled into 16,743 clusters (8,506 of which had one or more EST from the salivary gland library). Coding sequences were obtained for 2,509 novel proteins, 1,792 of which had at least one EST expressed in the salivary glands. Despite library normalization, 59 transcripts were overrepresented in the salivary library indicating high levels of expression. This work presents a detailed analysis of the salivary protein families identified. Protein expression was confirmed by 2D gel electrophoresis, enzymatic digestion and mass spectrometry. Concurrently, an initial attempt to determine the immunogenic properties of selected salivary proteins was undertaken.</p> <p>Conclusions</p> <p>The sialome of <it>G. m. morsitans </it>contains over 250 proteins that are possibly associated with blood feeding. This set includes alleles of previously described gene products, reveals new evidence that several salivary proteins are multigenic and identifies at least seven new polypeptide families unique to <it>Glossina</it>. Most of these proteins have no known function and thus, provide a discovery platform for the identification of novel pharmacologically active compounds, innovative vector-based vaccine targets, and immunological markers of vector exposure.</p

Evaluation of Serious Infection in Pediatric Patients with Low Immunoglobulin Levels Receiving Rituximab for Granulomatosis with Polyangiitis or Microscopic Polyangiitis

Introduction: The aim of this work was to assess the impact of prolonged low immunoglobulin (IgG or IgM) serum concentrations on the potential cumulative serious infection (SI) risk in pediatric patients following rituximab treatment for granulomatosis with polyangiitis or microscopic polyangiitis (GPA/MPA) in PePRS. Methods: Patients aged ≥ 2 to < 18 years received four weekly intravenous rituximab infusions of 375 mg/m2 and concomitant glucocorticoid taper. After 6 months, patients could receive further rituximab and/or other immunosuppressants per investigator discretion. Immunoglobulin levels and SIs were assessed throughout the 4.5-year observation period. Prolonged low IgG or IgM was defined as below the lower limit of normal age-specific reference range for ≥ 4 months. Results: A total of 25 patients were included, of whom 19 (76%) had GPA and six (24%) had MPA; 18 (72%) had newly diagnosed disease and seven (28%) had relapsing disease. All 25 patients completed the rituximab induction regimen; 24 completed ≥ 18 months of follow-up. At month 18, eighteen patients (72%) had prolonged low IgG; 19 (76%), prolonged low IgM; and 15 (60%), both. Seven patients (28%) had nine SIs; one occurred during or after prolonged low IgG only, two during or after prolonged low IgM only, and six during or after concurrent prolonged low IgG and IgM. No patients died or discontinued the study due to SI. All patients had complete and sustained peripheral B-cell depletion for ≥ 6 months. Conclusions: The majority of pediatric patients who received rituximab for GPA/MPA with prolonged low immunoglobulin levels did not experience SIs. In patients with SIs, these events were manageable, and the number of SIs did not increase over time or with multiple rituximab treatments. These observations are consistent with the rituximab safety profile in adults with GPA/MPA. Trial registration: ClinicalTrials.gov identifier, NCT01750697