Effects of Amino Acid and Branched-Chain Volatile Fatty Acid Additions on in Vitro Fermentation of Dormant Range Grasses

Two-stage in vitro fermentation was used to screen five amino acids and three branched-chain volatile fatty acids as potential additions to a grain urea supplement for cows grazing dormant winter range. Urea addition alone increased dry matter and fiber digestibility of dormant cool season grasses. Methionine addition improved fiber digestibility and rate of fermentation of cool season grasses over urea alone. Compared to urea addition, the branched-chain volatile fatty acids did not increase dry matter or fiber disappearance or improve rate of fermentation of dormant range grasses. None of the buffer additions tested or urea increased digestibility of the dormant warm season grasses. This preliminary laboratory study indicates that methionine offers the greatest potential for addition to a grain urea supplement to increase utilization of dormant range grasses

Evaluation of Wheat Middlings as a Supplement for Beef Cows Grazing Native Winter Range

Two winter grazing trials were conducted at the SDSU Cottonwood Research Station near Cottonwood, SD, to compare wheat middlings to soybean meal and corn-soybean meal supplements. In consecutive years, grazing trials from December to February were conducted using Simmental x Angus crossbred cows grazing two pastures with differing amounts of available forage and fed four supplemental treatments that were balanced to provide the following amounts of crude protein (Ib) and metabolizable energy (Mcal) per cow daily: 1) soybean meal .75 and 2.40, 2) low wheat middlings .75 and 4.76, 3) corn-soybean meal 1.50 and 9.40, and 4) high wheat middlings 1.50 and 9.40. Cows grazing the high available forage pasture gained 53 Ib more than those grazing the low available forage pasture. The supplement x pasture interaction indicates that level of available forage affects response to the supplemental treatment. When available forage was low, wheat middlings was a less effective source of supplemental protein than soybean meal. When available forage was high, soybean meal and the low wheat middlings supplements resulted in similar cow weight gains. Regardless of forage availability, the high wheat middlings supplement was a less effective source of supplemental energy cornpared to the corn-soybean meal supplement balanced to provide equal protein and energy. The supplement x year interaction resulted from soybean meal being more beneficial than low wheat middlings in year 1 while in year 2, soybean meal and low wheat middlings resulted in similar cow performance

Level of Available Forage and Supplemental Protein and Energy for Cows Grazing Winter Range

A winter grazing trial at the SDSU Range and Livestock Research Station near Cottonwood was conducted to determine if the response to feeding a high starch supplement is dependent on the amount of protein fed and the amount of forage available. During December and January 126 Simmental-Angus crossbred cows grazing two pastures with differing amounts of available forage were fed four supplemental treatments that provided the following amounts of crude protein (Ib) and metabolizable energy (Mcal) per cow daily: 1) .72 and 3.92, 2) .72 and 10.64, 3) 1.44 and 7.78, and 4) 1.44 and 10.91. Cows grazing the high available forage pasture gained 41 Ib more than those grazing the low forage pasture. lncreasing the amount of supplemental protein from .72 to 1.44 1b per cow daily increased cow gains. lncreasing the amount of supplemental energy did not improve cow weight gains when the level of supplemental protein was .72 1b per cow daily. When the amount of protein was doubled, increasing the amount of supplemental energy increased gains by 21 Ib. There was a tendency for a greater response to the higher protein, higher energy supplement for cows grazing the pasture with less forage available

Big bottlenecks in cardiovascular tissue engineering.

Although tissue engineering using human-induced pluripotent stem cells is a promising approach for treatment of cardiovascular diseases, some limiting factors include the survival, electrical integration, maturity, scalability, and immune response of three-dimensional (3D) engineered tissues. Here we discuss these important roadblocks facing the tissue engineering field and suggest potential approaches to overcome these challenges

An analysis of integrative outcomes in the Dayton peace negotiations

The nature of the negotiated outcomes of the eight issues of the Dayton Peace Agreement was studied in terms of their integrative and distributive aspects. in cases where integrative elements were Sound, further analysis was conducted by concentrating on Pruitt's five types of integrative solutions: expanding the pie, cost cutting, non-specific compensation, logrolling, and bridging. The results showed that real world international negotiations can arrive at integrative agreements even when they involve redistribution of resources tin this case the redistribution of former Yugoslavia). Another conclusion was that an agreement can consist of several distributive outcomes and several integrative outcomes produced by different kinds of mechanisms. Similarly, in single issues more than one mechanism can be used simultaneously. Some distributive bargaining was needed in order to determine how much compensation was required. Finally, each integrative formula had some distributive aspects as well

Relational considerations in the use of influence tactics.

It is proposed that the existing relationship between the influencing agent and the target of influence plays a central role in the choice of using hard and soft influence tactics. In a field study, 3 key aspects of the relation between agent and target were examined. and the results generally supported our hypotheses. First, the more unfairly people felt they were treated, the more often they wielded influence, especially using harder influence tactics. Second. the better the influencing agent liked the target. the relatively less often he or she used hard tactics. Finally, the more the influencing agent felt dependent upon the target, the fewer influence tactics, both hard and soft, were used. The discussion focuses on both the practical and theoretical implications of these findings

Evaluation of Wheat Middlings as a Supplement for Beef Cows Grazing Native Winter Range

A winter grazing trial was conducted at the SDSU Cottonwood Research Station near Cottonwood, SD, to compare wheat middlings to soybean meal and corn-soybean meal supplements. During December and January 122 pregnant Simmental-Angus crossbred cows grazing two pastures with differing amounts of available forage were fed four supplemental treatments that provided the following amounts of crude protein (Ib) and metabolizable energy (Mcal) per cow daily: I) soybean meal .75 and 2.40, 2) corn-soybean meal 1.50 and 9.40, 3) low wheat middlings .75 and 4.76, and 4) high wheat middlings 1.50 and 9.40. Cows grazing the high available forage pasture gained 56 Ib more than those grazing the low available forage pasture. Cows grazing the high available forage pasture were able to select a diet higher in crude protein and lower in acid = detergent fiber. The supplement x pasture interaction indicates that level of forage availability is a factor in determining a cow\u27s response to the supplemental treatment. When forage availability was low, wheat middlings was a less effective source of supplemental protein than soybean meal and a less effective source of supplemental energy compared to a corn-soybean meal supplement balanced to provide equal protein and energy. For cows grazing the high available forage pasture, soybean meal and the low wheat middlings supplements produced similar cow weight gains and the high wheat middlings supplement was a less effective source of supplemental energy than the corn-soybean meal supplement. Cows grazing the high forage pasture receiving 1.89 Ib soybean meal had similar weight gains and lower supplement cost than cows grazing the low forage pasture receiving 6.59Ib of the corn-soybean meal supplement

On Determining Dead Layer and Detector Thicknesses for a Position-Sensitive Silicon Detector

In this work, two particular properties of the position-sensitive, thick silicon detectors (known as the "E" detectors) in the High Resolution Array (HiRA) are investigated: the thickness of the dead layer on the front of the detector, and the overall thickness of the detector itself. The dead layer thickness for each E detector in HiRA is extracted using a measurement of alpha particles emitted from a $^{212}$Pb pin source placed close to the detector surface. This procedure also allows for energy calibrations of the E detectors, which are otherwise inaccessible for alpha source calibration as each one is sandwiched between two other detectors. The E detector thickness is obtained from a combination of elastically scattered protons and an energy-loss calculation method. Results from these analyses agree with values provided by the manufacturer.Comment: Accepted for publication in Nuclear Instruments and Methods in Physics Researc

DNA replication stress restricts ribosomal DNA copy number

Ribosomal RNAs (rRNAs) in budding yeast are encoded by ~100–200 repeats of a 9.1kb sequence arranged in tandem on chromosome XII, the ribosomal DNA (rDNA) locus. Copy number of rDNA repeat units in eukaryotic cells is maintained far in excess of the requirement for ribosome biogenesis. Despite the importance of the repeats for both ribosomal and non-ribosomal functions, it is currently not known how “normal” copy number is determined or maintained. To identify essential genes involved in the maintenance of rDNA copy number, we developed a droplet digital PCR based assay to measure rDNA copy number in yeast and used it to screen a yeast conditional temperature-sensitive mutant collection of essential genes. Our screen revealed that low rDNA copy number is associated with compromised DNA replication. Further, subculturing yeast under two separate conditions of DNA replication stress selected for a contraction of the rDNA array independent of the replication fork blocking protein, Fob1. Interestingly, cells with a contracted array grew better than their counterparts with normal copy number under conditions of DNA replication stress. Our data indicate that DNA replication stresses select for a smaller rDNA array. We speculate that this liberates scarce replication factors for use by the rest of the genome, which in turn helps cells complete DNA replication and continue to propagate. Interestingly, tumors from mini chromosome maintenance 2 (MCM2)-deficient mice also show a loss of rDNA repeats. Our data suggest that a reduction in rDNA copy number may indicate a history of DNA replication stress, and that rDNA array size could serve as a diagnostic marker for replication stress. Taken together, these data begin to suggest the selective pressures that combine to yield a “normal” rDNA copy number