Deformations of extended objects with edges

We present a manifestly gauge covariant description of fluctuations of a relativistic extended object described by the Dirac-Nambu-Goto action with Dirac-Nambu-Goto loaded edges about a given classical solution. Whereas physical fluctuations of the bulk lie normal to its worldsheet, those on the edge possess an additional component directed into the bulk. These fluctuations couple in a non-trivial way involving the underlying geometrical structures associated with the worldsheet of the object and of its edge. We illustrate the formalism using as an example a string with massive point particles attached to its ends.Comment: 17 pages, revtex, to appear in Phys. Rev. D5

Geometry of Deformations of Relativistic Membranes

A kinematical description of infinitesimal deformations of the worldsheet spanned in spacetime by a relativistic membrane is presented. This provides a framework for obtaining both the classical equations of motion and the equations describing infinitesimal deformations about solutions of these equations when the action describing the dynamics of this membrane is constructed using {\it any} local geometrical worldsheet scalars. As examples, we consider a Nambu membrane, and an action quadratic in the extrinsic curvature of the worldsheet.Comment: 20 pages, Plain Tex, sign errors corrected, many new references added. To appear in Physical Review

Open strings with topologically inspired boundary conditions

We consider an open string described by an action of the Dirac-Nambu-Goto type with topological corrections which affect the boundary conditions but not the equations of motion. The most general addition of this kind is a sum of the Gauss-Bonnet action and the first Chern number (when the background spacetime dimension is four) of the normal bundle to the string worldsheet. We examine the modification introduced by such terms in the boundary conditions at the ends of the string.Comment: 12 pages, late

Hamilton's equations for a fluid membrane: axial symmetry

Consider a homogenous fluid membrane, or vesicle, described by the Helfrich-Canham energy, quadratic in the mean curvature. When the membrane is axially symmetric, this energy can be viewed as an `action' describing the motion of a particle; the contours of equilibrium geometries are identified with particle trajectories. A novel Hamiltonian formulation of the problem is presented which exhibits the following two features: {\it (i)} the second derivatives appearing in the action through the mean curvature are accommodated in a natural phase space; {\it (ii)} the intrinsic freedom associated with the choice of evolution parameter along the contour is preserved. As a result, the phase space involves momenta conjugate not only to the particle position but also to its velocity, and there are constraints on the phase space variables. This formulation provides the groundwork for a field theoretical generalization to arbitrary configurations, with the particle replaced by a loop in space.Comment: 11 page

The Constraints in Spherically Symmetric General Relativity II --- Identifying the Configuration Space: A Moment of Time Symmetry

We continue our investigation of the configuration space of general relativity begun in I (gr-qc/9411009). Here we examine the Hamiltonian constraint when the spatial geometry is momentarily static (MS). We show that MS configurations satisfy both the positive quasi-local mass (QLM) theorem and its converse. We derive an analytical expression for the spatial metric in the neighborhood of a generic singularity. The corresponding curvature singularity shows up in the traceless component of the Ricci tensor. We show that if the energy density of matter is monotonically decreasing, the geometry cannot be singular. A supermetric on the configuration space which distinguishes between singular geometries and non-singular ones is constructed explicitly. Global necessary and sufficient criteria for the formation of trapped surfaces and singularities are framed in terms of inequalities which relate appropriate measures of the material energy content on a given support to a measure of its volume. The strength of these inequalities is gauged by exploiting the exactly solvable piece-wise constant density star as a template.Comment: 50 pages, Plain Tex, 1 figure available from the authors

The String Deviation Equation

The relative motion of many particles can be described by the geodesic deviation equation. This can be derived from the second covariant variation of the point particle's action. It is shown that the second covariant variation of the string action leads to a string deviation equation.Comment: 18 pages, some small changes, no tables or diagrams, LaTex2

Synthesis of Novel 6-(4-Substituted piperazine-1-yl)-9(b-D-ribofuranosyl) purine Derivatives, Which Lead to Senescence-Induced Cell Death in liver Cancer Cells

Cataloged from PDF version of article.Novel purine ribonucleoside analogues (9-13) containing a 4-substituted piperazine in the substituent at N-6 were synthesized and evaluated for their cytotoxicity on Huh7, HepG2, FOCUS, Mahlavu liver, MCF7 breast, and HCT116 colon carcinoma cell lines. The purine nucleoside analogues were analyzed initially by an anticancer drug-screening method based on a sulforhodamine B assay. Two nucleoside derivatives with promising cytotoxic activities (11 and 12) were further analyzed on the hepatoma cells. The N-6-(4-Trifluoromethylphenyl)piperazine analogue 11 displayed the best antitumor activity, with IC50 values between 5.2 and 9.2 mu M. Similar to previously described nucleoside analogues, compound 11 also interferes with cellular ATP reserves, possibly through influencing cellular kinase activities. Furthermore, the novel nucleoside analogue 11 was shown to induce senescence-associated cell death, as demonstrated by the SA beta-gal assay. The senescence-dependent cytotoxic effect of 11 was also confirmed through phosphorylation of the Rb protein by p15(INK4b) overexpression in the presence of this compound

Recapitulating cranial osteogenesis with neural crest cells in 3-D microenvironments

The experimental systems that recapitulate the complexity of native tissues and enable precise control over the microenvironment are becoming essential for the pre-clinical tests of therapeutics and tissue engineering. Here, we described a strategy to develop an in vitro platform to study the developmental biology of craniofacial osteogenesis. In this study, we directly osteo-differentiated cranial neural crest cells (CNCCs) in a 3-D in vitro bioengineered microenvironment. Cells were encapsulated in the gelatin-based photo-crosslinkable hydrogel and cultured up to three weeks. We demonstrated that this platform allows efficient differentiation of p75 positive CNCCs to cells expressing osteogenic markers corresponding to the sequential developmental phases of intramembranous ossification. During the course of culture, we observed a decrease in the expression of early osteogenic marker Runx2, while the other mature osteoblast and osteocyte markers such as Osterix, Osteocalcin, Osteopontin and Bone sialoprotein increased. We analyzed the ossification of the secreted matrix with alkaline phosphatase and quantified the newly secreted hydroxyapatite. The Field Emission Scanning Electron Microscope (FESEM) images of the bioengineered hydrogel constructs revealed the native-like osteocytes, mature osteoblasts, and cranial bone tissue morphologies with canaliculus-like intercellular connections. This platform provides a broadly applicable model system to potentially study diseases involving primarily embryonic craniofacial bone disorders, where direct diagnosis and adequate animal disease models are limited