50 research outputs found
Development of a hybrid model to interpolate monthly precipitation maps incorporating the orographic influence
[EN] This paper proposes an interpolation model for monthly rainfall in large areas of complex orography. It has been implemented in the Iberian Peninsula (continental territories of Spain and Portugal), Balearic and Canary Islands covering a territory of almost 600.000km(2). To do this a data set that comprises a total number of 11,822 monthly precipitation series has been created (11,042 provided by the Spanish Meteorological Agency and 780 provided by the National Water Resources Information System of the Portuguese Water Institute). The data set covers the period from October 1940 until September 2005. The interpolation model has been based on the assumption of two different components on monthly precipitation. The first component reflects local and seasonal characteristics and 24 different mean monthly precipitation maps (12) and SDs maps (12) compose it. It considers the varying influence of physiographic variables such as altitude and orientation. The second precipitation component reflects the synoptic pattern that dominated each month of the series and it is composed by series of anomalies of monthly precipitation (780). Anomalies have been interpolated by means of ordinary kriging once local spatial continuity was assumed. Gridded maps of each variable have been developed at 200m resolution following a hybrid methodology that implements two different interpolation techniques. The first technique applies a regression analysis to derive maps depending on altitude and orientation; the second one is a weighting technique to consider the non-linearity of the precipitation/altitude dependence. Cross validation has been applied to estimate the goodness of both techniques. Results show an average annual precipitation of 655mm/year. Although this figure is only 4% less than the estimate of MAGRAMA (2004), regional and local differences are highlighted when the spatial distribution is considered. The model constitutes a comprehensive implementation considering the availability of historical records and the need of avoiding slow calculations in large territories.Ministry of Economy, Industry and Competitiveness, Grant/Award Number: CGL2014-52571-RĂlvarez-RodrĂguez, J.; Llasat, M.; Estrela Monreal, T. (2019). Development of a hybrid model to interpolate monthly precipitation maps incorporating the orographic influence. International Journal of Climatology. 39(10):3962-3975. https://doi.org/10.1002/joc.6051S396239753910AEMET.2011Atlas ClimĂĄtico IbĂ©rico. (Iberian Climate Atlas) VV.AA. Agencia Estatal de MeteorologĂa. Ministerio de Medio Ambiente. ISBN: 978â84â7837â079â5. 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Stochastic simulation of precipitation-consistent daily reference evapotranspiration using vine copulas
Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)1.
In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field
Sphingosine 1-phosphate enhances the excitability of rat sensory neurons through activation of sphingosine 1-phosphate receptors 1 and/or 3
Coupling sensor observation services and web processing services for online geoprocessing in water dam monitoring
Novel sensor technologies are rapidly emerging. They enable a monitoring and modelling of our environment in a level of detail that was not possible a few years ago. However, while the raw data produced by these sensors are useful to get a first overview, it usually needs to be post-processed and integrated with other data or models in different applications. In this paper, we present an approach for integrating several geoprocessing components in the TaMIS water dam monitoring system developed with the Wupperverband, a regional waterbody authority in Germany. The approach relies upon the OGC Web Processing Service and is tightly coupled with Sensor Observation Service instances running at the Wupperverband. Besides implementing the standardized XML-based interface, lightweight REST APIs have been developed to ease the integration with thin Web clients and other Web-based components. Using this standards-based approach, new processing facilities can be easily integrated and coupled with different observation data sources
Loss of sphingosine 1-phosphate (S1P) in septic shock is predominantly caused by decreased levels of high-density lipoproteins (HDL)
Abstract Background Sphingosine 1-phosphate (S1P) is a signaling lipid essential in regulating processes involved in sepsis pathophysiology, including endothelial permeability and vascular tone. Serum S1P is progressively reduced in sepsis patients with increasing severity. S1P function depends on binding to its carriers: serum albumin (SA) and high-density lipoproteins (HDL). The aim of this single-center prospective observational study was to determine the contribution of SA- and HDL-associated S1P (SA-S1P and HDL-S1P) to sepsis-induced S1P depletion in plasma with regard to identify future strategies to supplement vasoprotective S1P. Methods Sequential precipitation of lipoproteins was performed with plasma samples obtained from 100 ICU patients: surgical trauma (nâ=â20), sepsis (nâ=â63), and septic shock (nâ=â17) together with healthy controls (nâ=â7). Resultant fractions with HDL and SA were analyzed by liquid chromatography coupled to triple-quadrupole mass spectrometry (LC-MS/MS) for their S1P content. Results Plasma S1P levels significantly decreased with sepsis severity and showed a strong negative correlation with increased organ failure, quantified by the Sequential Organ Failure Assessment (SOFA) score (rho ââ0.59, Pâ<â0.001). In controls, total plasma S1P levels were 208âÎŒg/L (187â216âÎŒg/L). In trauma patients, we observed an early loss of SA-S1P (ââ70%) with a concurrent increase of HDL-S1P (+â20%), resulting in unaltered total plasma S1P with 210âÎŒg/L (143â257âÎŒg/L). The decrease of plasma S1P levels with increasing SOFA score in sepsis patients with 180.2âÎŒg/L (123.3â253.0âÎŒg/L) and in septic shock patients with 99.5âÎŒg/L (80.2â127.2âÎŒg/L) was mainly dependent on equivalent reductions of HDL and not SA as carrier protein. Thus, HDL-S1P contributed most to total plasma S1P in patients and progressively dropped with increasing SOFA score. Conclusions Reduced plasma S1P was associated with sepsis-induced organ failure. A constant plasma S1P level during the acute phase after surgery was maintained with increased HDL-S1P and decreased SA-S1P, suggesting the redistribution of plasma S1P from SA to HDL. The decrease of plasma S1P levels in patients with increasing sepsis severity was mainly caused by decreasing HDL and HDL-S1P. Therefore, strategies to reconstitute HDL-S1P rather than SA-S1P should be considered for sepsis patients