626 research outputs found
Phase separation in transparent liquid-liquid miscibility gap systems
A program to be carried out on transparent liquid-phase miscibility gap materials was developed for the purpose of acquiring additional insight into the separation process occurring in these systems. The transparency feature allows the reaction to be viewed directly through light scattering and holographic methods
Materials science experiments in space
The criteria for the selection of the experimental areas and individual experiments were that the experiment or area must make a meaningful contribution to the field of material science and that the space environment was either an absolute requirement for the successful execution of the experiment or that the experiment can be more economically or more conveniently performed in space. A number of experimental areas and individual experiments were recommended for further consideration as space experiments. Areas not considered to be fruitful and others needing additional analysis in order to determine their suitability for conduct in space are also listed. Recommendations were made concerning the manner in which these materials science experiments are carried out and the related studies that should be pursued
Calibration of Gas Flow Meters using Choked Flow and an Evacuated Vessel
The measurement of gas flow rates is of great importance in a wide range of modern technologies. This paper introduces a simple, yet accurate technique for in-house calibration of gas FMs (mass and volumetric) even under harsh environmental conditions such as encountered during field measurement campaigns. The method requires only readily available, low cost components: a vessel of known volume, an air pump, a pressure sensor and a metal plate orifice or a needle valve to act as a CO. The unique property of choked flow in the CO is used here for flow calibration. In the method presented here a vessel is evacuated to below the critical pressure ( \u3c 0.53 of upstream pressure) and then allowed to refill with ambient air (or some other process gas) under so-called choked flow conditions through the CO. The method presented here leverages that the flow rate upstream of the CO is not only constant but readily determined from (a) the known V VESS, (b) the measured time rate of change of the absolute pressure in the vessel and (c) the ideal gas law. This calculated flow rate can be used for calibration of FMs. The accuracy of the method depends only on the accuracy of the pressure measurement, the timer and the value of the V VESS. The flow rate computed in this way is found to be in excellent agreement (typically 1% difference) with the flow rate measured by a soap film FM (Gilibrator). As expected from theory this method is found to work for all kinds of CFRs (here: various types of metal plate orifices and needle valves were tested), gas types (here: air, Argon, and CO2) and upstream pressures (here: between 650 hPa and 1400 hPa). The accuracy of this technique (∼1%) is as good as that of standard volume displacement methods (e.g. soap film FMs) (typically 1% difference), the standard of laboratory-based flow calibrators, but less expensive and more suitable for harsh environments
Quantum Eavesdropping without Interception: An Attack Exploiting the Dead Time of Single Photon Detectors
The security of quantum key distribution (QKD) can easily be obscured if the
eavesdropper can utilize technical imperfections of the actual implementation.
Here we describe and experimentally demonstrate a very simple but highly
effective attack which even does not need to intercept the quantum channel at
all. Only by exploiting the dead time effect of single photon detectors the
eavesdropper is able to gain (asymptotically) full information about the
generated keys without being detected by state-of-the-art QKD protocols. In our
experiment, the eavesdropper inferred up to 98.8% of the key correctly, without
increasing the bit error rate between Alice and Bob significantly. Yet, we find
an evenly simple and effective countermeasure to inhibit this and similar
attacks
Percolation in the classical blockmodel
Classical blockmodel is known as the simplest among models of networks with
community structure. The model can be also seen as an extremely simply example
of interconnected networks. For this reason, it is surprising that the
percolation transition in the classical blockmodel has not been examined so
far, although the phenomenon has been studied in a variety of much more
complicated models of interconnected and multiplex networks. In this paper we
derive the self-consistent equation for the size the global percolation cluster
in the classical blockmodel. We also find the condition for percolation
threshold which characterizes the emergence of the giant component. We show
that the discussed percolation phenomenon may cause unexpected problems in a
simple optimization process of the multilevel network construction. Numerical
simulations confirm the correctness of our theoretical derivations.Comment: 7 pages, 6 figure
Calibration of Tethered Particle Motion Experiments
The Tethered Particle Motion (TPM) method has been used to observe and characterize a variety of protein-DNA interactions including DNA loping and transcription. TPM experiments exploit the Brownian motion of a DNA-tethered bead to probe biologically relevant conformational changes of the tether. In these experiments, a change in the extent of the bead’s random motion is used as a reporter of the underlying macromolecular dynamics and is often deemed sufficient for TPM analysis. However, a complete understanding of how the motion depends on the physical properties of the tethered particle complex would permit more quantitative and accurate evaluation of TPM data. For instance, such understanding can help extract details about a looped complex geometry (or multiple coexisting geometries) from TPM data. To better characterize the measurement capabilities of TPM experiments involving DNA tethers, we have carried out a detailed calibration of TPM magnitude as a function of DNA length and particle size. We also explore how experimental parameters such as acquisition time and exposure time affect the apparent motion of the tethered particle. We vary the DNA length from 200 bp to 2.6 kbp and consider particle diameters of 200, 490 and 970 nm. We also present a systematic comparison between measured particle excursions and theoretical expectations, which helps clarify both the experiments and models of DNA conformation
Single metallic nanoparticle imaging for protein detection in cells
We performed a visualization of membrane proteins labeled with 10-nm gold
nanoparticles in cells, using an all-optical method based on photothermal
interference contrast. The high sensitivity of the method and the stability of
the signals allows 3D imaging of individual nanoparticles without the drawbacks
of photobleaching and blinking inherent to fluorescent markers. A simple
analytical model is derived to account for the measurements of the signal
amplitude and the spatial resolution. The photothermal interference contrast
method provides an efficient, reproducible, and promising way to visualize low
amounts of proteins in cells by optical means
Single-molecule experiments in biological physics: methods and applications
I review single-molecule experiments (SME) in biological physics. Recent
technological developments have provided the tools to design and build
scientific instruments of high enough sensitivity and precision to manipulate
and visualize individual molecules and measure microscopic forces. Using SME it
is possible to: manipulate molecules one at a time and measure distributions
describing molecular properties; characterize the kinetics of biomolecular
reactions and; detect molecular intermediates. SME provide the additional
information about thermodynamics and kinetics of biomolecular processes. This
complements information obtained in traditional bulk assays. In SME it is also
possible to measure small energies and detect large Brownian deviations in
biomolecular reactions, thereby offering new methods and systems to scrutinize
the basic foundations of statistical mechanics. This review is written at a
very introductory level emphasizing the importance of SME to scientists
interested in knowing the common playground of ideas and the interdisciplinary
topics accessible by these techniques. The review discusses SME from an
experimental perspective, first exposing the most common experimental
methodologies and later presenting various molecular systems where such
techniques have been applied. I briefly discuss experimental techniques such as
atomic-force microscopy (AFM), laser optical tweezers (LOT), magnetic tweezers
(MT), biomembrane force probe (BFP) and single-molecule fluorescence (SMF). I
then present several applications of SME to the study of nucleic acids (DNA,
RNA and DNA condensation), proteins (protein-protein interactions, protein
folding and molecular motors). Finally, I discuss applications of SME to the
study of the nonequilibrium thermodynamics of small systems and the
experimental verification of fluctuation theorems. I conclude with a discussion
of open questions and future perspectives.Comment: Latex, 60 pages, 12 figures, Topical Review for J. Phys. C (Cond.
Matt
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