93 research outputs found
Eta-mesic nuclei
In this contribution we report on theoretical studies of nuclear
quasi-bound states in few- and many-body systems performed recently by the
Jerusalem-Prague Collaboration [1-5]. Underlying energy-dependent
interactions are derived from coupled-channel models that incorporate the
resonance. The role of self-consistent treatment of the strong
energy dependence of subthreshold amplitudes is discussed. Quite large
downward energy shift together with rapid decrease of the amplitudes
below threshold result in relatively small binding energies and widths of the
calculated nuclear bound states. We argue that the subthreshold behavior
of scattering amplitudes is crucial to conclude whether nuclear
states exist, in which nuclei the meson could be bound and if the
corresponding widths are small enough to allow detection of these
nuclear states in experiment.Comment: 7 pages, 5 figures; presented at HADRON2017, Sept. 25-29, 2017,
Salamanca (Spain); prepared for Proceedings of Scienc
Specific Rna Self-assembly With Minimal Paranemic Motifs
The paranemic crossover (PX) is a motif for assembling two nucleic acid molecules using Watson-Crick (WC) basepairing without unfolding preformed secondary structure in the individual molecules. Once formed, the paranemic assembly motif comprises adjacent parallel double helices that crossover at every possible point over the length of the motif. The interaction is reversible as it does not require denaturation of basepairs internal to each interacting molecular unit. Paranemic assembly has been demonstrated for DNA but not for RNA and only for motifs with four or more crossover points and lengths of five or more helical half-turns. Here we report the design of RNA molecules that paranemically assemble with the minimum number of two crossovers spanning the major groove to form paranemic motifs with a length of three half turns (3HT). Dissociation constants (K-d\u27s) were measured for a series of molecules in which the number of basepairs; between the crossover points was varied from five to eight basepairs. The paranemic 3HT complex with six basepairs (3HT_6M) was found to be the most stable with K-d = 1 x 10(-8) M. The half-time for kinetic exchange of the 3HT_6M complex was determined to be similar to 100 min, from which we calculated association and dissociation rate constants k(a) = 5.11 x 10(3) M(-1)s(-1) and k(d) = 5.11 x 10(-5) s(-1). RNA paranemic assembly of 3HT and 5HT complexes is blocked by single-base substitutions that disrupt individual intermolecular Watson-Crick basepairs; and is restored by compensatory substitutions that,restore those basepairs. The 3HT motif appears suitable for specific, programmable, and reversible tecto-RNA self-assembly for constructing artificial RNA molecular machines
Renal oncocytoma: a comparative clinicopathologic study and fluorescent in-situ hybridization analysis of 73 cases with long-term follow-up
Clinical studies have confirmed that renal oncocytoma (RO) is a benign neoplasm with excellent prognosis. In diagnostically challenging cases of renal oncocytic epithelial neoplasms, fluorescent in-situ hybridization (FISH) is increasingly being used and its ability to distinguish RO from chromophobe renal cell carcinoma (ChRCC) has been documented. In this study, we evaluated the differential diagnostic contribution of FISH in cases of RO
Separable potential model for interactions at low energies
The effective separable meson-baryon potentials are constructed to match the
equivalent chiral amplitudes up to the second order in external meson momenta.
We fit the model parameters (low energy constants) to the threshold and low
energy data. In the process, the -proton bound state problem is
solved exactly in the momentum space and the 1s level characteristics of the
kaonic hydrogen are computed simultaneously with the available low energy
cross sections. The model is also used to describe the
mass spectrum and the energy dependence of the amplitude.Comment: 31 pages, v2 - added corrections to make it compatible with the
published versio
Elastic Pion Scattering on the Deuteron in a Multiple Scattering Model
Pion elastic scattering on deuterium is studied in the KMT multiple
scattering approach developed in momentum space. Using a Paris wave function
and the same methods and approximations as commonly used in pion scattering on
heavier nuclei excellent agreement with differential cross section data is
obtained for a wide range of pion energies. Only for MeV and very
backward angles, discrepancies appear that are reminiscent of disagreements in
pion scattering on He, H, and He. At low energies the second order
corrections have been included. Polarization observables are studied in detail.
While tensor analyzing powers are well reproduced, vector analyzing powers
exhibit dramatic discrepancies.Comment: 25 pages LATEX and 9 postscript figures in a self-extracting uufile
archiv
The reaction on p-shell nuclei
This letter is concerned with the study of the reaction in p-shell nuclei, i.e., , ,
and . The emission rates are
reported as a function of . These rates are discussed in comparison with
previous findings. The ratio in p-shell nuclei is
found to depart largely from that on hydrogen, which provides support for large
in-medium effects possibly generated by the sub-threshold . The
continuum momentum spectra of prompt pions and free sigmas are also discussed
as well as the missing mass behavior and the link with the
reaction mechanism. The apparatus used for the investigation is the FINUDA
spectrometer operating at the DANE -factory (LNF-INFN, Italy).Comment: 14 pages, 5 figures, accepted for publication in Phys. Lett.
Pion interaction with the trinucleon up to the eta production threshold
Pion elastic, charge exchange scattering and induced eta production on the
trinucleon systems are investigated in a coupled-channels approach in momentum
space with Fadeev wave functions. The channel is
included using an isobar model with S-, P-, and D-wave resonances. While the
coherent reactions like He(He can be reasonably well reproduced
up to =500 MeV, large discrepancies appear for the incoherent
processes, He(H and He(H at backward
angles and energies above -resonance. In the forward direction the
calculations underestimate the experimental measurements very
close to threshold but agreement with the data improves with increasing pion
energy. Predictions are made for the asymmetries of the various reactions on
polarized He.Comment: 40 pages, 12 figures (available from the authors), Mainz preprint
MKPH-T-92-1
Correlated pairs from the reaction
Correlated pairs emitted after the absorption of negative kaons
at rest in light nuclei and are
studied. -hyperons and deuterons are found to be preferentially
emitted in opposite directions. The invariant mass spectrum of
shows a bump whose mass is 32516 MeV/c. The bump mass (binding
energy), width and yield are reported. The appearance of a bump is discussed in
the realm of the [] clustering process in nuclei. The experiment was
performed with the FINUDA spectrometer at DANE (LNF).Comment: 13 pages, 5 figures, accepted for publication in Phys. Lett.
Glutamine depletion by crisantaspase hinders the growth of human hepatocellular carcinoma xenografts
Background:
A subset of human hepatocellular carcinomas (HCC) exhibit mutations of ÎČ-catenin gene CTNNB1 and overexpress Glutamine synthetase (GS). The CTNNB1-mutated HCC cell line HepG2 is sensitive to glutamine starvation induced in vitro with the antileukemic drug Crisantaspase and the GS inhibitor methionine-L-sulfoximine (MSO).
Methods:
Immunodeficient mice with subcutaneous xenografts of the CTNNB1-mutated HCC cell lines HepG2 and HC-AFW1 were treated with Crisantaspase and/or MSO, and tumour growth was monitored. At the end of treatment, tumour weight and histology were assessed. Serum and tissue amino acids were determined by HPLC. Gene and protein expression were estimated with RT-PCR and western blot and GS activity with a colorimetric method. mTOR activity was evaluated from the phosphorylation of p70S6K1.
Results:
Crisantaspase and MSO depleted serum glutamine, lowered glutamine in liver and tumour tissue, and inhibited liver GS activity. HepG2 tumour growth was significantly reduced by either Crisantaspase or MSO, and completely suppressed by the combined treatment. The combined treatment was also effective against xenografts of the HC-AFW1 cell line, which is Crisantaspase resistant in vitro.
Conclusions:
The combination of Crisantaspase and MSO reduces glutamine supply to CTNNB1-mutated HCC xenografts and hinders their growth
Maternal DNA Methylation Regulates Early Trophoblast Development
This work was funded by the BBSRC, Wellcome Trust, MRC, the EC Network of Excellence EpiGeneSys, the EC BLUEPRINT project, the Centre for Trophoblast Research, and CIHR (grant MOP-119357 to L.L.). M.R.B. is a Sir Henry Dale Fellow (101225/Z/13/Z), jointly funded by the Wellcome Trust and the Royal Society
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