59 research outputs found
The significance of measuring monocyte tissue factor activity in patients with breast and colorectal cancer
Monocytes express tissue factor (mTF) in several conditions including cancer where levels may be valuable in assessing tumour presence and progression. Using a two-stage kinetic chromogenic assay (KCA), mTF levels were measured in controls [normal subjects (n = 60) and patients undergoing hernia repair or cholecystectomy (n = 60)], in patients with benign and malignant disease of the breast (n = 83) and of the large bowel (n = 62). This was performed under fresh (resting) conditions and after incubation for 6 h without (unstimulated) and with (stimulated) Escherichia coli endotoxin. The malignant groups showed higher mTF levels than each of the three controls for resting (P < 0.05 breast, P < 0.05 colorectal) unstimulated (P < 0.05 breast, P < 0.05 colorectal) and stimulated cells (P < 0.001 breast, P < 0.01 colorectal). Similarly, the benign inflammatory groups had higher mTF levels than controls for resting (P < 0.05 colorectal), unstimulated (P < 0.05 colorectal) and stimulated cells (P < 0.01 breast, P < 0.01 colorectal). There was no significant difference between malignant and benign inflammatory groups in each organ. mTF levels showed an increase corresponding to that of histological tumour progression and were higher in non-surviving patients. In conclusion, mTF levels are raised in malignant and inflammatory disease compared to controls and patients with non-inflammatory conditions. Stimulated cells give better discrimination between the groups and may be of value in identifying high risk individuals. mTF levels showed an association with tumour grade or stage and the patients' survival time
The TNF Receptors p55 and p75 Mediate Chemotaxis of PMN Induced by TNFα and a TNFα 36–62 Peptide
The present study was performed to examine whether residues
36–62 of TNFα contain the chemotactic domain of
TNFα, and whether the p55 and p75 TNF receptors are involved
in TNFα induced chemotaxis. The chemotactic effect of
TNFα on PMN was inhibited by the mAbs Hrt-7b and Utr-1,
against the p55 and p75 TNF receptors, respectively. Both receptors may
therefore be required for mediating the chemotactic effect of TNFcz.
The synthetic TNFα 36–62, similar to TNFα, had
chemotactic effects on both PMN and monocytes. The chemotactic
activity of the TNFα 36–62 peptide on PMN, was inhibited
by Htr-7b, Utr-1 and soluble p55 receptor, which shows that the
peptide possessed the ability to induce chemotaxis through the TNF
receptors. In contrast to TNFα, the peptide did not show a
cytotoxic activity against WEHI 164 flbrosarcoma cells. It is
suggested that different domains of the TNFα molecule induce
distinct biological effects
Lipopolysaccharide Induced Monocyte Thromboplastin Synthesis and Coagulation Responses in Patients Undergoing Coronary Bypass Surgery after Preoperative Supplementation with n-3 Fatty Acids
Purification and Properties of an Abnormal Blood Coagulation Factor IX (Factor IXBm)/Kinetics of Its Inhibition of Factor X Activation by Factor VII and Bovine Tissue Factor
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