Transformation of settlement history in the Körös Region in the period between the Late Bronze Age and the end of Iron Age

Abstract of PhD thesis submitted in 2020 to the Archaeology Doctoral Programme, Doctoral School of History, Eötvös Loránd University, Budapest under the supervision of Gábor V. Szabó

Characterisation of Streptomycin resistant mutants of biocontrol Bacillus strains

A good antibiotic-producing Bacillus subtilis and an elevated extracellular enzyme-secreting Bacillus amyloliquefaciens biocontrol strain were investigated. The B. subtilis strain produces non-ribosomal oligopeptides: iturin, surfactin and fengycin. These cyclic lipopeptides have antifungal and antibacterial effects. The B. amyloliquefaciens strain secrets many extracellular enzymes (proteases, chitinases, cellulases and lipases); these have great significance in the antagonism. Kurosawa and co-workers reported elevated protease and P-amylase secretion from streptomycin-resistant B. subtilis mutants. We isolated twenty spontaneous streptomycin resistant mutants from our B. subtilis strain and four spontaneous streptomycin resistant mutants from the B. amyloliquefaciens strain. We investigated the extracellular enzyme and antibiotic production of the mutants. Some of the mutants showed elevated enzyme and antibiotic secretion. The rpsL gene in theses spontaneous streptomycin resistant mutants, were sequenced and point mutations were detected in it, so very likely the changes of the structure of the rpsL protein is responsible in some cases for the elevated enzyme and depsipeptide production

EXPERIMENTELLE BESTIMMUNG DES DYNAMISCHEN VERHALTENS EINER KREISELPUMPE BEIM AUSLAUFEN

The primary condition of transporting liquids in pipelines is the installation of a properly matched pump. The calculations of operation parameters in both steady-state and transient flow conditions require the mathematical model of the pump. The currently used model is based on the well-known steady-state equations and characteristics of the pump [1], [2]. Both experimental results and theoretical considerations suggest that this model is inaccurate under transient flow conditions and requires further development. The first step of creating a more accurate mathematical model is to make experiments to find out how the characteristics of the pump change under transient flow conditions. The research was conducted within the framework of OTKA T029073 R& D scheme

Determination of Load Equivalency Factors by Statistical Analysis of Weigh-In-Motion Data

The load equivalency factors for pavement design currently in use by the Hungarian standard have been developed using Weigh-in-Motion data obtained during the first few years of operations after installing some 30 measuring sites in Hungary in 1996. In the past years, and currently, data is collected mainly at the border crossings of the country, however the data is used only for law enforcement purposes, and no comprehensive statistical analyses have been done. To develop actual load equivalency factors for the use in pavement design, data of one year was collected and statistical methods were applied. An algorithm was used to help managing the multimodal distribution of axle loads in mathematical perspectives. Monte-Carlo methods were applied to determine the factors for each heavy vehicle type and eventually for each vehicle class used by the current Hungarian pavement design manual. The calculated factors are considerably different from the current ones, indicating that the pavement design may lead to a false result. Furthermore, there are three vehicle types suggested to be incorporated into the standard due to their high occurrence

Development of a novel electrochemical method for the detection of invertase enzyme in honey samples

Invertase (α-glucosidase) is one of the most important honey enzymes; it hydrolyses sucrose into fructose and glucose during honey ripening process. Next to the basic honey ingredients (glucose, fructose, water), invertase activity is one of the main characterising parameter of honey: it can be used as indicator of aging and/or overheating, but it also may give information about adulteration. Our aim was to develop a novel analytical method for the fast determination of invertase activity that can be used during quality control of honey samples. Our assay based on the application of an artificial substrate, namely p-nitrophenyl-α-D-glucopyranoside. p-Nitrophenol produced by the enzyme reaction is detected by amperometric method which is much more sensitive than the traditional spectrophotometric determination. Screen-printed carbon electrodes and a potentiostat were used for amperometric measurement. Our measuring system worked in flow injection system. The measuring parameters (polarization potential, pH etc.) were optimized. The applicability of the method was tested for detection of α-glucosidase enzyme activity