Standardization of the bio-active compounds (rotenoids) from the extract of local plant species (derris elliptica) using the internal standard method of high performance liquid chromatography (HPLC)

It is well known now that some plant species represent an efficient factory of chemicals, which are manufactured and used as bio-weapons against pest attacks. Extensive work has been done during the last few decades on these potentially useful compounds. During the last few decades a growing interest has been paid for safe agricultural production i.e free residual toxicity hazards to human beings and to the environment. Plant extracts-based biocides possess a great advantage compared with the chemical ones. Their efficacies are also acceptable. Research carried out was to standardize and determine the bio-active compounds from the extract of local plant species (Derris elliptica) using the internal standard method of the isocratic High Performance Liquid Chromatography (HPLC) analysis system. The raw plants were collected from Kota Johor Lama, Johor and sorted to collect the root and stem. Only the root and stem were utilized as a raw material of the extraction process. The root and stem were extracted by using the Normal Soaking Extraction (NSE) method at 28 0C to 30 0C with 95.0 % (v/v) of acetone as a solvent and the solvent-to-solid ratio of the extraction is (10.0 ml/g). The extraction was carried out for 24 hours and further cleaned up to remove fine debris of root and stem prior to determination of the rotenone and its derivatives content. The rotenone cube resin of SAPHYR S.A.R.L (France) was used to verify the appearances of the compounds in the extract. The employed method of analysis shows significant appearances of the bio-active compounds in the extract compared with the commercial grade of rotenone cube resin

Blind image separation based on exponentiated transmuted Weibull distribution

In recent years the processing of blind image separation has been investigated. As a result, a number of feature extraction algorithms for direct application of such image structures have been developed. For example, separation of mixed fingerprints found in any crime scene, in which a mixture of two or more fingerprints may be obtained, for identification, we have to separate them. In this paper, we have proposed a new technique for separating a multiple mixed images based on exponentiated transmuted Weibull distribution. To adaptively estimate the parameters of such score functions, an efficient method based on maximum likelihood and genetic algorithm will be used. We also calculate the accuracy of this proposed distribution and compare the algorithmic performance using the efficient approach with other previous generalized distributions. We find from the numerical results that the proposed distribution has flexibility and an efficient resultComment: 14 pages, 12 figures, 4 tables. International Journal of Computer Science and Information Security (IJCSIS),Vol. 14, No. 3, March 2016 (pp. 423-433

A study into the effect of concentration process on the yield of rotenone from the extract of local plant species (Derris elliptica)

Bio-pesticides are becoming increasingly important as pest management tools in various cropping systems in the tropics essentially to remedy problems associated with the indiscriminate use of ‘hard’ inorganic pesticide and interest in organic agriculture. In the few decades, many bio-pesticidal products, both microbial-based (bacteria, fungi, microsprodia, entomopathogenic nematodes, viruses) and plant-based botanicals (rotenone and azadiracthin) had been studied for use against insect pests in the tropics. In this paper, the effect of the concentration process towards the yield of rotenone; mg and its concentration; mg/ml are studied extensively. The raw plants were collected from Kota Johor Lama, Johor and sorted to collect the root and stem. Only the root and stem were utilized as a raw material of the extraction process. The root and stem were extracted using the Normal Soaking Extraction (NSE) at 28 0C to 30 0C with 95 % (v/v) of acetone as a solvent and the solvent-to-solid ratio of 10 ml/g). The extraction was carried out for 24 hours. The liquid crude extract was concentrated further (the solvent removed under reduced pressure) using the rotary evaporator at 50 0C and 80 mbar of vacuum pressures. The fractions of the liquid crude extract were collected for each interval time (15 mins/1.0 ml/fraction). Each fractions were diluted 1/100 with acetone and further cleaned up prior to determination of rotenone content; mg and concentration; mg/ml by using the High Performance Liquid Chromatography (HPLC). Significant effect of the concentration process against the yield of rotenone; mg was recorded and shows a significant thermal degradation or dissipation of rotenone content at higher operating temperature. The possibilities for better exploitation and identification of the effective operating parameters will be discussed

Normal soaking extraction (NSE) of rotenone from Derris elliptica

Derris elliptica or the tuba plant contains rotenone, bio-active compound known that has the potential to be used as bio-pesticide. Bio-pesticide is the best-known alternative bio-pesticide which has the potential to replace the use of conventional pesticides, as it is more environmentally friendly. The main objective of the paper is to obtain the best extraction solvent for optimizing the yield in rotenone extraction. Rotenone was extracted from Derris root using three different parts of roots and three types of solvent in Normal Soaking Extraction (NSE). The types of Derris roots are: (1) Fine root (2) Coarse root (3) Stem. The three types of solvent system are: (1) Acetone 95 % (v/v), (2) Chloroform 99.9 % (v/v) and (3) a mixture of ethanol: H2O (9:1) added with oxalic acid (1mg/ml). The liquid crude extracts were further cleaned up to remove the fine debris of roots. The presence of rotenone was confirmed using qualitative analysis Thin Layer Chromatography (TLC) and thereupon the determination of rotenone content was carried out using High Performance Liquid Chromatography (HPLC). From the results obtained, it was found that the Normal Soaking Extraction (NSE) using acetone 95 % (v/v) was the best method to extract the highest yield of rotenone; 1.14 % (w/w)

Quantum Monte Carlo simulation of overpressurized liquid 4He

A diffusion Monte Carlo simulation of superfluid $^4$He at zero temperature and pressures up to 275 bar is presented. Increasing the pressure beyond freezing ($\sim$ 25 bar), the liquid enters the overpressurized phase in a metastable state. In this regime, we report results of the equation of state and the pressure dependence of the static structure factor, the condensate fraction, and the excited-state energy corresponding to the roton. Along this large pressure range, both the condensate fraction and the roton energy decrease but do not become zero. The roton energies obtained are compared with recent experimental data in the overpressurized regime.Comment: 5 pages, accepted for publication in Phys. Rev. Let

Effect of Permeable Vessel Closure and Gelling Agent on Reduction of Hyperhydricity in in Vitro Culture of Carnation

Hyperhydricity, an abnormal morphological appearance and physiologicalfunction, is an important problem in carnation tissue culture. The problem causes premature flowering, high occurrence of abnormal shoots, difficulty in transferring hyperhydric plantlets to soil, and low survival rate of plantlets. High relative humidity and the water potential are considered as the key factors involved in the abnormality. Furthermore, permeable culture vessel and gelling agent were assured to be high potential treatment to eliminate it. Objective of this research was to reduce hyperhydricity in regenerants of carnation using different permeable vessel closures and gelling agents and to assess the multiplication and acclimatization abilities of recovered shoots. Experiment was arranged in randomized complete block design with four replications. First factor was different types of closure, i.e. cotton wool, plastic wrap, parafilm and aluminium foil, while second one was gelling agents, i.e. bacto agar, phytagel, swallow agar, and Type 900 agar. The recovered shoots were then multiplied, rooted, and acclimatized. The results showed that hyperhydricity was successfully reduced by applying permeable closure (cotton wool and plastic wrap) in combination with Type 900 agar. The combination of plastic wrap and Type 900 agar was the most appropriate treatment in reducing hyperhydricity and producing good quality shoots. The treatment reduced the problem down to 23% of total condition of hyperhydricity (100%) and increased leaf chlorophyll content from 0.0883 to 0.1288 mg mg-1. The plastic wrap was easily applied and cheaper material compared to cotton wool. The recovered shoots were able to produce 1-3 healthy axillary shoots and easily rooted on half-strength MS. The recovered plantlets were simply acclimatized with survival rate up to 100% on kossas peat + soil (1:1, v/v) and flowered 4-5 months after acclimatization with decreasing in number and size of flower

Identifikasi Tanaman Buah Tropika Berdasarkan Tekstur Permukaan Daun Menggunakan Jaringan Syaraf Tiruan

Indonesia merupakan salah satu negara dengan keanekaragaman tanaman buah tropika yang cukup tinggi. Keanekaragaman tanaman buah tropika tersebut merupakan satu tantangan dalam melakukan identifikasi. Identifikasi tanaman dapat dilakukan berdasarkan buah, bunga, maupun daun. Identifikasi berdasarkan daun merupakan identifikasi yang lebih mudah dilakukan karena daun akan ada sepanjang masa, sedangkan bunga dan buah mungkin hanya ada pada waktu tertentu. Identifikasi tanaman menggunakan daun dapat dilakukan berdasarkan bentuk, tekstur, maupun warna citra daun tersebut. Pada penelitian ini, ekstraksi fitur gray level co-occurrence matrix (GLCM) dari tekstur citra permukaan daun buah tropika digunakan sebagai input dari pelatihan Jaringan syaraf tiruan untuk proses identifikasi. Secara keseluruhan, pengujian dengan menggunakan hidden neuron sebanyak 7 menghasilkan hasil akurasi terbaik, yaitu 90%

High-quality variational wave functions for small 4He clusters

We report a variational calculation of ground state energies and radii for 4He_N droplets (3 \leq N \leq 40), using the atom-atom interaction HFD-B(HE). The trial wave function has a simple structure, combining two- and three-body correlation functions coming from a translationally invariant configuration-interaction description, and Jastrow-type short-range correlations. The calculated ground state energies differ by around 2% from the diffusion Monte Carlo results.Comment: 5 pages, 1 ps figure, REVTeX, submitted to Phys. Rev.