Identification of Anaerobic Bacteria in Iranian patients with Rheumatoid Arthritis

Please cite this article as: Shafeian SH, Bandehpour M, Mirzaahmadi S, Tofighi A. Identification of Anaerobic Bacteria in patients with Rheumatoid Arthritis. . Novel Biomed 2013;1(2):39-42.Background: There is a report about the 2-5% prevalence for septic arthritis by anaerobic bacteria. The relevance between synivitis and intestinal microbial flora has been a hypothesis for ten years. The PCR with sensitivity and specificity 99% for microorganism detection in acute, chronic and relapse form of septic arthritis is helpful.Methods: In this research we designed to diagnosis intestinal anaerobic bacteria which are able to occur bacteremia or septicemia. So amplification of the 16srRNA and narG genes in this type of bacteria is the best way for detection of them and followed by the treatment of the patients.Results: 100 patients with septic arthritis incidences were studied here. From these numbers 61% were bacterial arthritis and 18% were infected by anaerobic bacteria. Conclusion: On the base of coding of nitrate reductase, the positive samples were identified Enterobacter cloacae and Methylovorus sp. O157   Escherichia coli and Borrelia garinii

Anion-directed assemblies of Cu(II) mono, di, and poly-nuclear coordination compounds with a 32-membered azacrown ligand: Synthesis, characterisation and crystal structures

A novel 32-membered azacrown ligand L was synthesized from the [2 + 2] condensation between 2-[3-(2-formylphenoxy)-2-hydroxy propoxy]benzaldehyde and 1,2-diaminopropane by following in situ reduction. Three metal-organic coordination compounds $[CuL(NCS)]NO_3·CH_3OH (1), [Cu_2L(CH_3COO)_4].0.5_H2O (2)$, and $[CuL(μ-Cl)(NO_3)]_n.2nCH_3CN (3)$, were prepared from reaction of L with copper(II) salts containing different anions. Depending on the reaction controlling factor, i.e. $NCS^−, CH_3COO^−$, , and $Cl^−$anions, mono, di and polynuclear metal-organic coordination compounds were constructed. Complexes were characterized by spectroscopic methods and single crystal X-ray diffraction. Crystal structures indicate that L in presence of $NCS^−$ and $Cu(NO_3)_2·3H_2O$ forms mononuclear complex 1. Dinuclear complex 2 was formed by the reaction of L in presence of $Cu(CH_3COO)_2·2H_2O$. Reaction of $Cu(NO_3)_2·3H_2O$ and $CuCl_2·3H_2O$ with L give rise complex 3 which is a 1-dimensional coordination polymer. The coordination polymer constructed from distorted octahedron shaped copper nucleus that are exomacrocylic in which two Cl atoms serve as bridge. Potentially decadentate ligand, L, with four N- and six O-donor sites in presence of Cu(II) salts reacts only with its N-donors. Complexes 1 and 3, both, are crystalized in a centrosymmetric space group $P2_{1/c}$ and dinuclear complex 2 is crystalized in a polar space group $P2_1$