Shoulder chondromatosis with multiple loose bodies and glenoid erosion- managed by arthroscopic loose body removal and BMAC

Chondromatosis is a rare, benign condition of the joints with metaplasia of synovium into cartilage leading to the formation of loose bodies. Involvement of the shoulder joint is uncommon. Symptomatic individuals require removal of the loose bodies. Here we describe a case report of a patient with synovial chondromatosis of the shoulder with multiple loose bodies causing glenoid cartilage erosions and loss. This required not only loose body removal but cartilage regenerative procedure- bone marrow aspirate concentrate (BMAC) application. Post-surgery, he had a rapid resolution of symptoms

A short term analysis of external rotation deficit following a combined arthroscopic bankart with remplissage and rotator interval closure for anteroinferior instability with subcritical bone loss

Background: Our study aims at assessing the clinical outcomes of our patients who have undergone arthroscopic bankart and remplissage along with rotator interval closure in the treatment of anteroinferior instability with subcritical bone loss, with focus on external rotation deficit. Methods: This is a single-center retrospective study including 48 consecutive patients who underwent surgery Arthroscopic Bankarts with remplissage procedure for anterior shoulder instability with Hill-Sachs lesion. In our study, the minimum follow-up was 6 months and the average follow-up of was 21months. At the last follow-up, active range of motion, western ontario shoulder instability index (WOSI) and University of California at Los Angeles (UCLA) scores were assessed. Results: 48 patients who underwent surgery in the time period of 2019-2023 who completed minimum 6months follow up were included in the study. The average age of the patients was 31.7 years (range, 16-52 years), with 93.8% male patients and 6.2% female patients. The range of motion at follow-up was comparable with the normal side, with loss of terminal external rotation in 2 patients (4%). Average University of California at Los Angeles score was 30.7 and Western Ontario shoulder instability index was 3.8%. One patient had only one episode of subluxation and there was one case of infection. Conclusions: The results of our study validate the combination of Remplissage and rotator interval closure with Bankart repair in the treatment of anterior instability with glenoid bone loss <15% irrespective of tracking of Hill Sachs lesion for excellent functional outcomes, least rate of recurrence and without significant loss of external rotation

Some observations on the trend of zooplankton and Its probable influence on local pelagic fisheries at Colachel during 1973-74

The zooplankton standing crop showed three peaks of abundance, in March, September and December, the first two peaks during the periods of high salinity. In the order of abundance, the forms which constituted the bulk of the plankton were copepods, cladocerans, cirrepede larvae, Lucifer, chaetognaths and appendicularians. The copepod distribution was found to have characters common both to that of the adjacent coastal waters of Bay of Bengal on the southeast coast and to that of the Arabian sea on the southwest coast. The peak occurrence of cladocerans during May-July was followed by the appearance of juvenile mackerels in early August. Pelagic fish catch, consisting mostly of anchovies and lesser sardines, showed clear peaks, closely following the primary and secondary peaks of plankton. The peak occurrence of anchovies during September-October seems to be in prey-predator relationship with ribbonfish, the peak of which followed closely

Effect of different UCOE-promoter combinations in creation of engineered cell lines for the production of Factor VIII

<p>Abstract</p> <p>Background</p> <p>The most common approach used in generating cell lines for the production of therapetic proteins relies on gene amplification induced by a drug resistance gene e. g., DHFR and glutamine synthetase. Practically, this results in screening large number of clones for the one that expresses high levels of the biologic in a stable manner. The inefficiency of mammalian vector systems to express proteins in a stable manner typically involves silencing of the exogenous gene resulting from modifications such as methylation of CpG DNA sequences, histone deacetylation and chromatin condensation. The use of un-methylated CpG island fragments from housekeeping genes referred to as UCOE (ubiquitous chromatin opening elements) in plasmid vectors is now well established for increased stability of transgene expression. However, few UCOE-promoter combinations have been studied to date and in this report we have tested 14 different combinations.</p> <p>Findings</p> <p>In this report we describe studies with two different UCOEs (the 1.5 Kb human RNP fragment and the 3.2 Kb mouse RPS3 fragment) in combination with various promoters to express a large protein (B domain deleted factor VIII; BDD-FVIII) in a production cell line, BHK21. We show here that there are differences in expression of BDD-FVIII by the different UCOE-promoter combinations in both attached and serum free suspension adapted cells. In all cases, the 1.5 Kb human RNP UCOE performed better in expressing BDD-FVIII than their corresponding 3.2 Kb mouse RPS3 UCOE. Surprisingly, in certain scenarios described here, expression from a number of promoters was equivalent or higher than the commonly used and industry standard human CMV promoter.</p> <p>Conclusion</p> <p>This study indicates that certain UCOE-promoter combinations are better than others in expressing the BDD-FVIII protein in a stable manner in BHK21 cells. An empirical study such as this is required to determine the best combination of UCOE-promoter in a vector for a particular production cell line.</p

Production Ecology of a Tropical Sandy Beach at Benaulim, Goa

163-167Species composition, biomass and available food of the intertidal fauna were studied at monthly intervals. Fluctuations in the production of macrofauna were related to environmental stress and availability of food. In the premonsoon months (February-May), faunal diversity and biomass were maximum with a marked zonation on the beach. During monsoon (June-September), the beach was characterized by the presence of tidal migrants which could withstand changing sea conditions. Three feeding types, viz. feeders on plankton and suspended matter, deposit feeders and scavengers and predators, were represented. Annual requirement of carbon for the macrofauna was estimated to be 2574.4 gC/m2 transect. An attempt to understand trophic relationship indicated sufficient food from direct sources to meet the demand

Tilapia Fry and Fingerling Production in Small Tanks

Two experiments were conducted co evaluate the use of small tanks as a hatchery for the production of tilapia fry and fingerlings. The effect of stocking density of brood fish on fry production was evaluated using a sex ratio of four females to one male. Brood fish (Tilapia aurea) were stocked at rates of 2.7, 5.4 and 8.1 Fish/m2 in nylon spawning nets (hapas) suspended in tanks. Fry were collected seven times at 2-week intervals. Mean fry production was 12.2, 15.1 and 23.8/m2/hapa/day or 5.3, 3.9 and 3.8/female/day, respectively. The fry were stocked at races of 26, 52, 78, 104, 130 and 155/m2 to determine the optimum rate for fingerling production. The fry were fed four times daily for 63 days. The daily feeding rate was 15% of initial body weight for three weeks followed by weekly adjustments to 5%. Predation by dragonfly larvae caused wide variation in survival (23-88%). Fry at the 26/m2 rate grew fastest, averaging 2.3 g with 37% survival. 13.5% actual feeding rate and 2.3 feed conversion ratio. Fry at the 104/m2 rate weighed 1.2 g with 88% survival, 6% actual feeding rate and 0.75 feed conversion ratio. The data indicates that the daily feeding rate should be higher than 5% of body weight for maximum growth but should not exceed 40 kg/ha/day in small static tanks

Extra-adrenal retroperitoneal paraganglioma associated with duplication of inferior vena cava

Retroperitoneal paragangliomas arise from specialized neural crest cells symmetrically distributed along the aorta in association with the sympathetic chain. If this tissue aggregates in the adrenal medulla pheochromocytoma may arise. When it remains in the paraaortic sites it could develop into extra-adrenal, retroperitoneal paraganglioma. We report a case of extra-adrenal paraganglioma in the renal hilum intimately related to the left side of a double inferior vena cava. To the best of our knowledge such an association has never been described before. The clinical significance of this venous anomaly is reviewed

Growth of the Bean Clam <i>Donax incarnatus</i> Gmelin from a Sandy Beach at Benaulim, Goa

197-199Growth rate of Donax incarnatus was determined for 1 yr in relation to ecological factors and meristic characters. Average monthly growth rate was 2.2 mm. Growth of newly settled animals was faster than the older populations. Growth was influenced by the availability of particulate organic carbon and chlorophyll a in the surf water. From the multiregressional analysis it was observed that 2 variables of meristic characters gave better estimates of growth than a single variable