Expression of enhancer of zeste homolog 2 correlates with survival outcome in patients with metastatic breast cancer: exploratory study using primary and paired metastatic lesions

Evaluation of immunostaining of EZH2 and Ki67. (DOC 52 kb

A地区の自主防災力を高める保健師の支援

A地区における防災に関するニーズをもとに開催した防災講習会が住民の意識に与える影響および自主防災力を高めるための保健師の支援について検討した。地域の組織・団体へのインタビュー調査やA地区住民に対するアンケー結果をもとに、ニーズを反映した講習会を開催し、開催後にアンケートを実施した。各地域の組織・団体は「自主防災組織の発展」、「市や消防団との連携体制」を望んでいた。また、住民のニーズや防災講習会をとおして、①平均自立期間延伸のための取り組み、②住民組織間の連携の強化、③人材発掘、④ニーズの把握、⑤継続的かつ体験型の防災活動の実施等の保健師の支援が必要といえた

O-Glycan-Altered Extracellular Vesicles: A Specific Serum Marker Elevated in Pancreatic Cancer

Pancreatic cancer (PC) is among the most lethal malignancies due to an often delayed and difficult initial diagnosis. Therefore, the development of a novel, early stage, diagnostic PC marker in liquid biopsies is of great significance. In this study, we analyzed the differential glycomic profiling of extracellular vesicles (EVs) derived from serum (two cohorts including 117 PC patients and 98 normal controls) using lectin microarray. The glyco-candidates of PC-specific EVs were quantified using a high-sensitive exosome-counting system, ExoCounter. An absolute quantification system for altered glycan-containing EVs elevated in PC serum was established. EVs recognized by O-glycan-binding lectins ABA or ACA were identified as candidate markers by lectin microarray. Quantitative analyses using ExoCounter revealed that the ABA- or ACA-positive EVs were significantly increased in the culture of PC cell lines or in the serum of PC patients including carbohydrate antigen 19-9 negative patients with high area under curve values. The elevated numbers of EVs in PC serum returned to normal levels after pancreatectomy. Histological examination confirmed that the tumors stained with ABA/ACA. These specific EVs with O-glycans recognized by ABA/ACA are elevated in PC sera and can act as potential biomarkers in a liquid biopsy for PC patients screening