Effects of carvacrol on a human non-small cell lung cancer (NSCLC) cell line, A549

15th Annual Meeting of the Japanese-Association-for-Animal-Cell-Technology -- NOV 11-15, 2002 -- Fuchu, JAPANWOS: 000222558600036Carvacrol, the predominant monoterpene in many essential oils of Labitae including Origanum, Satureja, Thymbra, Thymus, and Corydothymus has substantial antibacterial, anti fungal, antihelmintic, insecticial, analgesic and antioxidant activities. In this study a human non-small cell lung cancer (NSCLC) cell line, A549 was used. Approximately 75% of lung cancer is non-small cell carcinoma (NSCLC) which comprises several histologic types: squamous cell, adenocarcinoma and large cell carcinoma. It was reported that the proportion of lung tumors diagnosed as adenocarcinoma has increased. For this reason A549 cell line was chosen for this study. To investigate the effects of carvacrol on cell morphology, apoptosis and total protein amount, the cells were incubated with various concentration of carvacrol in DMSO for 24 hours. In carvacrol applied A549 cell line, increase in dose of carvacrol caused a decrease in cell number, degeneration of cell morphology and a decrease in total protein amount. To characterize carvacrol induced changes in cell morphology, cells were examined by light microscopy. Cells were treated with carvacrol were seen to have detached from the disk, with cell rounding, cytoplasmic blebbing and irregularity in shape. The data demonstrate that carvacrol is very potent inhibitor of cell growth in A549 cell line.Japanese Assoc Anim Cell Techno

Cytotoxic effects of various lactic acid bacteria on Caco-2 cells

Probiotics are live microbial food supplements that can be considered a functional food. They benefit the health of a host animal by maintaining their intestinal microbial balance. Most probiotic microorganisms are lactic acid bacteria (LAB) such as Lactobacillus spp., Bifidobacterium spp., and Enterococcus spp. LAB have been reported to possess certain anticancer properties. The vast majority of studies on their anticancer effects have dealt with colorectal cancers, although there have also been some studies on breast and bladder cancers. Colon cancer is the fourth most common cause of cancer-related mortality in the world. The aim of this study was to investigate the antiproliferative effects of the cell-free filtrate and the cell-free lyophilized filtrate of 3 LAB (Pediococcus pentosaceus, Lactobacillus plantarum, and Weissella confusa) on human colorectal adenocarcinoma cell line Caco-2. The filtrates were found to inhibit the growth of colon cancer cells in a dose-dependent manner as detected by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, suggesting that these strains might have use as probiotics in functional food or for colon cancer treatment. There are no other studies related to the anticancer activities of W. confusa in the literature

Differential effect of green tea catechins on three endothelial cell clones isolated from rat adipose tissue and on human umbilical vein endothelial cells

WOS: 000229896800004PubMed ID: 19003256By single colony isolation from the cells in stromal vascular fraction (SVF) dispersed from rat adipose tissues, we isolated three independent clones with different proliferation potential. All clones showed cobblestone-like morphology at the confluence and incorporated fluorescent Dil acetylated low density lipoprotein. When plated on Matrigel, they formed a capillary network-like structure. These rat adipose tissue endothelial cell (RATEC) clones showed higher expression of wnt2, wnt4, wnt5a, wnt5b, fzd1 and fzd5 whereas lower expression of cell cycle controlling genes such as CIP1, KIP1, KIP2, CDKN2A, CDKN2B, CDKN2C and CDKN2D compared to human umbilical vein endothelial cell (HUVEC). As reported for HUVEC, the growth of RATEC was inhibited by green tea catechins such as epigallocatechin, epicatechin gallate, epicatechin and epigallocatechin gallate but with higher sensitivity than HUVEC. The sensitivity of RATEC to catechins was higher for the cultures with low plating density and for the clone with higher proliferation potential

Dill seed oil as a possible contraceptive agent: antiangiogenic effects on endothelial cells

Dill (Anethum graveolens L.) essential oil is wide spread in the food, beverage and pharmaceutical sectors. Dill is a member of the Apiaceae (Umbelliferae) family. It has the following biological activities: antioxidant, antifungal, antibacterial, antimicrobial, antihyperlipidemic, antihypercholesterolemic, antispasmodic, antiproliferative and anti-inflammatory. Aqueous extract of dill seed has reported effects on sex hormones and infertility potential. Moreover, boiled dill seed has an impact on reducing labor duration in giving birth. Implantation and placentation are necessary for a healthy pregnancy in the early stages. Angiogenesis is responsible for these essential processes. This study aimed to investigate dill seed oil’s cytotoxic and antiangiogenic effects on rat adipose tissue endothelial cells (RATECs). Dill seed oil showed dose-dependent cytotoxicity on RATECs. It disrupted endothelial tube formation and depolymerized F-actin stress fibers. According to this study, depolymerization of F-actin stress fiber by dill seed oil could inhibit angiogenesis by suppressing endothelial cell proliferation, tube formation and motility. In other words, dill seed oil can be a new anti-angiogenic agent and a novel contraceptive

Cryptic fragment ?4 LG4-5 derived from laminin ?4 chain inhibits de novo adipogenesis by modulating the effect of fibroblast growth factor-2

PubMed: 18067585Cleavage of the extracellular matrix (ECM) by proteolysis unmasks cryptic sites and generates novel fragments with biological activities functionally distinct from those of the intact ECM molecule. The laminin G-like (LG)4-5 fragment has been shown to be excised from the laminin ?4 chain in various tissues. However, the functional role of this fragment has remained unknown to date. To investigate this, we prepared ?4 LG1-3 and ?4 LG4-5 fragments by elastase digestion of recombinant ?4 LG1-5, and examined their effects on de novo adipogenesis in mice at the site of injection of basement membrane extract (Matrigel) and fibroblast growth factor (FGF)-2. Although the addition of whole ?4 LG1-5 suppressed adipogenesis to some extent, the ?4 LG4-5 fragment could strongly suppress adipogenesis at a concentration of less than 20 nm. Addition of the ?4 LG4 module, which contains a heparin-binding region, had a suppressive effect, but this was lost in mutants with reduced heparin-binding activity. In addition, antibodies against the extracellular domain of syndecan-2 and -4, which are known receptors for the ?4 LG4 module, suppressed adipogenesis. Thus, these results suggest that the cryptic ?4 LG4-5 fragment derived from the laminin ?4 chain inhibits de novo adipogenesis by modulating the effect of FGF-2 through syndecan

In Vitro Evaluation of Gilaburu (Viburnum Opulus L. ) Juice on Different Cell Lines

The purpose of this study is to investigate the effects of gilaburu juice on cell viability (3- (4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl tetrazolium bromide, MTT) and angiogenesis (tube formation assay) using different cell lines (human cancer cell lines A549, Caco-2, HeLa and normal cell lines MDCK and HUVEC) in vitro. In addition, the genotoxic effects of gilaburu juice is evaluated using COMET assay on HUVEC cells. Our results demonstrate that gilaburu juice could inhibit the growth of Caco-2 and HeLa cancer cell lines, but could not significantly inhibit normal cell lines and A549 cancer cell lines. It disrupted tube formation of HUVEC cells. Gilaburu juice appears to have no genotoxic potential to the DNA of HUVEC cells. The results obtained in this study confirm the potential application of commercial gilaburu juice as a functional food in prevention of cancer

Anti-angiogenic and antiproliferative properties of the lichen substances (-)-usnic acid and vulpinic acid

WOS: 000359348000006PubMed ID: 26136299The anti-proliferative activities of the lichen substances (-)-usnic acid and vulpinic acid on the viability of HepG2 hepatocarcinoma cells, NS20Y neuroblastoma cells and HUVEC endothelial cells were studied by the MTT assay. The anti-angiogenic potential of the substances was determined by the endothelial tube formation assay. Both lichen substances exhibited strong anti-angiogenic activity and were more cytotoxic to the cancer cell lines than to the normal cell line, but vulpinic acid has more potential as an anti-angiogenic substance because of its low cytotoxicity and stronger anti-angiogenic activity on the HUVEC cell line

The in vitro assessment of dipyridophenazine complexes in H-ras oncogene transformed rat embryo fibroblast 5RP7 cell line

WOS: 000444475200002PubMed ID: 29313280Purpose The aim of this study is to detect apoptotic and cytotoxic/antiproliferative effects of a ligand substance and its metal derivatives. The substances were investigated by using an h-ras oncogene transformed rat embryo fibroblast cell line (5RP7). Methods The cytotoxic influences of dipyrido[3,2-a:2,3'c]phenazine ligand, dipyrido[3,2-a:2,3'c] phenazine-platinum(II) complex ([Pt(dppz)Cl-2]) and dipyrido[3,2-a:2,3'c] phenazine-gold(III) complex ([Au(dppz)Cl-2]Cl) were determined with MTT (3[4,5-dimetiltiyazol2-yl]-2,5-difeniltetrazolyum bromid) assay on 5RP7 cells. Results Dipyrido[3,2-a:2,3'c] phenazine, dipyrido[3,2-a:2,3'c] phenazine-platinum(II) complex ([Pt(dppz)Cl-2]) and dipyrido[3,2-a:2,3'c] phenazine-gold(III) complexes ([Au(dppz)Cl-2]Cl) caused significant increase in cytotoxicity in a dose and time dependent manner. The effects of dipyridophenazine ligand (dppz) and its metal derivatives on apoptosis were monitorized using cytotoxic dose (10M) DAPI fluorescent staining. It was shown that dppz and its compounds induced apoptosis. Conclusions These findings show that dpzz and its complexes can be studied as novel alternative chemotherapeutics in cancer treatment.Scientific and Technological Research Council of Turkey [TUBITAK-SBAG-108S206]This work was financed by a grant from the Scientific and Technological Research Council of Turkey (TUBITAK-SBAG-108S206). The authors are grateful to Anadolu University and Bezmialem University. We would like to thank Barlas Benkli due to contributions related to corrections of english language

Promotion of Hair Growth by Traditionally Used Delphinium Staphisagria Seeds through Induction of Angiogenesis

WOS: 000377527300018PubMed ID: 27642326How the seeds of Delphinium staphisagria promote hair growth in humans is yet to be discovered. This lack of information leads us to the investigation of hair promoting effects of seeds of Delphinium staphisagria in-vitro. Extract prepared from the Seed of Delphinium staphisagria (ESDS) - traditionally used for hair loss treatment - was selected and tested for the cytotoxic and angiogenic potential in endothelial cells (HUVECs) and human keratinocytes (HaCaT) cells. The effects of extract was determined by using in-vitro colorimetric MTT [3-(4,5-dimethythiazol-2-yl)-2,5-diphenyltetrazolium bromide] cytotoxicity assay. To identify the compounds that induce angiogenesis, we applied matrigel capillary assay in-vitro using HUVECs. Vinegar and water extracts of D. staphisagria seeds significantly promoted the proliferation of human keratinocyte cells by 137, 139, 143, 149 and 147 % at the concentration of 100, 120, 200, 250 and 300 mu g/mL compared with vehicle -treated control, respectively at 24 h. HUVECs viability remained the same with the control group at the concentration 1, 10, 20 and 40 mu g/mL after 24 h. Results demonstrated that ESDS did not cause toxicity in human keratinocytes and endothelial cells, while inducing the angiogenic activity in-vitro. D. staphisagria seeds promote hair growth without overt cytotoxicity and through inducing angiogenesis. Based on the information from the traditional uses and our experimental results, D. staphisagria's seeds appear to be a good candidate for the promotion of hair growth