Karaciğer yağlanmasına karşı kullanılan bitkisel preparatlar: Sistematik derleme

The fatty liver disease is defined as more than 5% of liver histiocytes filled with fatty vacuoles, depending on diet and indepen- dent of alcohol consumption. As it is known, fatty liver and related dis- orders are common, but still have no effective treatment. It is one of the important health problems in developed countries, associated with social and economic problems. Non-alcoholic fatty liver disease (NAFLD) is among the most common chronic metabolic disorders. It was observed that the prevalence of NAFLD has increased significantly worldwide in recent years. With advancing obesity epidemics, NAFLD has become the most common cause of chronic liver disease both in adults and children. Therefore, the clinical and economic burden of the disease is remarkable and increasing within the health system. While fatty liver is a metabolic disorder that has no definite drug treatment today, there are some traditional preparations used for this purpose as ethnobotanically. According to ethnobotanical studies in the current lit- erature associated to liver diseases; various in vitro, in vivo, or clinical experimental studies were reported with successful results. Thus, in this present review, studies on natural sourced raw materials, herbal for- mulations related to liver diseases, especially fatty liver, obesity, hy- perlipidemia were compiled mainly from ethnobotanical origin.Beslenmeye bağlı, alkol tüketiminden bağımsız karaciğer his- tiositlerinin %5’ten fazlasının yağ vakuolleriyle dolu olması “yağlı ka- raciğer hastalığı” olarak tan ımlanmaktadır. Bilindiği üzere karaci ğer yağlanması ve karaciğer yağlanmasına bağlı rahatsızlıklar sık görülen ancak günümüzde kanıtlanmış etkin bir tedavisi olmayan metabolik ra- hatsızlıklardır. Ülkemizde de önemli sa ğlık sorunlarının başında gel- mekte olup, sosyal ve ekonomik problemleri beraberinde getirmektedir. Nonalkolik yağlı karaciğer hastalığı, gelişmiş ülkelerde en yaygın kro- nik metabolik rahats ızlıklar arasındadır. Son y ıllarda alkole ba ğlı ol- mayan yağlı karaciğer hastalığı prevalansının dünya genelinde önemli bir şekilde arttığı gözlenmiştir. İlerleyen obezite salgınları ile nonalko- lik yağlı karaciğer hastalığı, erişkinlerde ve çocuklarda kronik karaci- ğer hastalığının en yayg ın nedeni hâline gelmi ştir. Bu nedenle ya ğlı karaciğer hastalığının klinik ve ekonomik yükü dikkate de ğerdir. Ka- raciğer yağlanması, günümüzde kesin bir ilaç tedavisi olmayan bir me- tabolik rahats ızlık iken, halk aras ında bu amaçla kullan ılan baz ı geleneksel preparatlar mevcuttur. Karaciğer rahatsızlıklarında bitkiler etnobotanik çalışmalarda yer almış; çeşitli in vitro, in vivo veya klinik deneysel araştırmalarda yer almış olup, başarılı sonuçlar alınmıştır. Bu derlemede, bilhassa karaciğer yağlanması, obezite, hiperlipidemi başta olmak üzere karaciğer rahatsızlıkları ile ilişkili bitkiler, bitkisel formü- lasyonlar ve bitkilerden elde edilen do ğal kaynaklı ham maddeler ile yapılan çalışmalar listelenmiştir. Gerek halk arasında kullanımları içe- ren, gerek deneysel çalışmaları (in vivo ve klinik çalışmalar) içeren ma- kaleler derlenmiş ve özetlenmiştir

ACE2 and LOX enzyme inhibitions of different lavender essential oils and major components linalool and camphor

In this present study, Lavandula angustifolia, Lavandula stoechas, and Lavandula x heterophylla essential oils and their main compounds linalool and camphor were evaluated in vitro for lipoxygenase enzyme (LOX) and for angiotensin converting enzyme 2 (ACE2) inhibition potential. The chemical compositions of L. angustifolia, L. stoechas, and L. heterophylla essential oils were confirmed both by gas chromatography-mass spectrometry and gas chromatography-flame ionization detection, where 22.4, 0.9, and 30.6% linalool and 17.8, 54.7, and 15% camphor were identified for each oil among other components, respectively. Enzyme inhibitory activity studies were performed at 20 mu g/mL for the tested essential oils, whereas for linalool and camphor concentrations, 5 mu g/mL was used. The ACE2 inhibitions of L. angustifolia, L. stoechas, and L. heterophylla essential oils were 25.4, 34.1, and 27.1%, while the LOX inhibitions were observed as 79, 49.1, and 86.7%, respectively. In addition, linalool and camphor showed remarkable ACE2 inhibition with 77.1 and 85.1%, whereas the LOX inhibition was observed at 92 and 67.2%, respectively. In conclusion of the initial findings, further detailed in vivo studies are needed to confirm the safe use.Anadolu University Scientific Research Projects Commissio

Self-transcendence among adults 65 years and older : A meta-analysis

Introduction Self-transcendence is a human capacity for wellbeing by expanding one's personal boundaries and may act as a health-promoting resource among adults ≥65 years. Therefore, the objectives of this meta-analysis were to determine the mean score of self-transcendence based on place of residence and gender, and to evaluate the correlations of self-transcendence with meaning, sense of coherence, resilience and depression. Methods Based on inclusion criteria, 13 studies were included. Orwin Safe N and Egger's test assessed publication bias. The mean score of self-transcendence and the correlation coefficients of the selected variables were estimated by random effects models. Results The self-transcendence mean score (n = 1634) was low (M = 43.6) and a bit lower among those staying in care facilities (M = 42.8), but did not vary significantly across gender. The correlation coefficients were self-transcendence_depression (r = −0.40), self-transcendence_meaning-in-life (r = 0.53), self-transcendence_resilience (r = 0.50) and self-transcendence_sense of coherence (r = 0.28). The correlation coefficients, except for meaning-in-life, were homogeneous. Conclusion In a health-promoting perspective, the concept of self-transcendence can help to better understand wellbeing among older individuals and provide guidance for health professionals in facilitating wellbeing and health. The concept and theory of self-transcendence can inspire health professionals in realising new health-promoting approaches to support older individuals in maintaining health, wellbeing and independency.publishedVersio

Harmonic resonance phenomena on nonlinear SH waves

The interaction of shear horizontal (SH) waves in a two layered elastic medium and its mth harmonic component is studied. The dispersion relation is analysed to obtain the wave number-phase velocity pairs where the third and fifth harmonic resonance phenomena emerge. By employing an asymptotic perturbation method it is shown that the balance between the weak nonlinearity and dispersion yields a coupled nonlinear Schrödinger (CNLS) equation for the slowly varying amplitudes of the fundamental wave and its fifth harmonic component. The nonlinearity effects of the materials and the ratio of layers’ thicknesses on the linear instabilities of solutions and the existence of solitary waves are examined.Publisher's VersionQ4WOS:00096429890000

Ergenlerde Okul Tükenmişliği ve Okul Bağlılığı: Facebook Bağımlılığının Aracı ve Farklılaştırıcı Rolü

In this study, the mediating and moderating role of Facebook addiction in the relationship between school burnout, and school engagement was investigated. The study group comprised of 283 students continuing to 6th, 7th, 8th and 9th grades. The data were collected by School Burnout Inventory, School Engagement Scale and Facebook Addiction Scale. As a result of the correlation analysis, a significant positive correlation was found between school burnout and Facebook addiction. Also, school engagement was negatively related to school burnout and Facebook addiction. The structural equation model findings indicated that Facebook addiction had a partial mediating role between school burnout and school engagement. The results of the bootstrap analysis supported the significance of the partial mediating role. Besides, as a result of the regression analysis, it was found that Facebook addiction had a differentiating role in the relationship between burnout and school engagement. Research findings are discussed in light of the literature. It is recommended to carry out studies to prevent social media addiction and to enhance conscious technology use. These studies are considered to contribute to reduce school burnout of the students and increase their school engagement.Bu çalışmada Facebook bağımlılığının okul tükenmişliği ve okul bağlılığı arasındaki ilişkide aracı ve farklılaştırıcı rolü araştırılmıştır. Çalışmanın katılımcıları 6., 7., 8. ve 9. sınıfta öğrenim gören 283 öğrenciden oluşmaktadır. Veriler Okul Tükenmişliği Ölçeği, Facebook Bağımlılığı Ölçeği ve Okul Bağlılığı Ölçeği aracılığıyla toplanmıştır. Korelasyon analizi sonucunda okul tükenmişliği ve Facebook bağımlılığı arasında anlamlı düzeyde pozitif ilişki bulunmuştur. Okul bağlılığı ise okul tükenmişliği ve Facebook bağımlılığı ile anlamlı düzeyde negatif ilişkilidir. Yapısal Eşitlik Modeli sonuçları okul tükenmişliği ve okul bağlılığı arasındaki ilişkide Facebook bağımlılığının kısmi aracı rolü olduğunu göstermektedir. Bootstrap analizi sonuçları kısmi aracı rolün anlamlılığını desteklemektedir. Ayrıca regresyon analizi sonucunda okul tükenmişliği ve okul bağlılığı arasındaki ilişkide Facebook bağımlılığının farklılaştırıcı rolü olduğu bulunmuştur. Sonuçlar alanyazın eşliğinde tartışılmıştır. Sosyal medya bağımlılığı önlemeye ve teknolojinin bilinçli kullanımını geliştirmeye yönelik çalışmaların yapılması önerilmektedir. Bu çalışmaların öğrencilerin okul tükenmişliğinin azalmasına ve okula olan bağlılıklarının artmasına katkısı olabileceği düşünülmektedir

Phlomis Pungens’in fitokimya ve in vitro farmakolojik etkilerinin değerlendirilmesi

Objective: This study aimed to investigate the in vitro wound healing, anti-inflammatory, antimicrobial and antioxidant activity of Phlomis pungens Willd. extract derived from the aerial parts. Material and Method: The phytochemical analysis was performed using GC-MS in order to identify the volatile components of the bioactive Hex extract. The wound healing activity of P. pungens extract was evaluated based on in vitro antimicrobial, antioxidant, anti-inflammatory, and, scratch activity was studied. In addition, the in vitro cytotoxicity of the extract was also evaluated. Result and Discussion: P. pungens methanol extract depicted a 5-LOX inhibitory activity at 78.2µg/mL (IC50), while the antioxidant activity by DPPH radical provided an IC50=2.41mg/mL, and the ABTS radical showed IC50=3.32mg/mL, respectively. The extract showed dose-dependently anti-inflammatory activity while L-NAME and P. pungens methanol extract significantly decreased LPS stimulated PGE2 production. According to the scratch assay results, all treatments led to an increase in cell migration rate with a dose-dependent effect. Our findings suggested that P. pungens methanol extract may have a role in wound healing according to the scratch test, and it is thought that its antioxidant and anti-inflammatory activity also contributed. Further evaluations are ongoing to confirm the in vitro activity under in vivo conditions.Amaç: Bu çalışmada, Phlomis pungens Willd. topraküstü kısımlarından elde edilen ekstrelerin in vitro yara iyileşmesi, antiinflamatuar, antimikrobiyal ve antioksidan aktivitesinin araştırılması amaçlanmıştır. Gereç ve Yöntem: Biyoaktif hekzan ekstresinin uçucu bileşenlerini belirlemek için fitokimyasal analiz GC-MS kullanılarak yapılmıştır. P. pungens ekstresinin yara iyileştirme aktivitesi, in vitro antimikrobiyal, antioksidan, antiinflamatuar etkinlikleri değerlendirilmiş ve ek olarak ekstrenin in vitro sitotoksisitesi de değerlendirilmiştir. Sonuç ve Tartışma: P. pungens metanol ekstresi, 78,2 µg/mL'de (IC50) 5-LOX inhibe edici aktivite gösterirken, DPPH yöntemi ile antioksidan aktivitesi IC50=2.41mg/mL ve ABTS IC50=3.32 mg/mL olarak bulunmuştur. Ekstre, doza bağlı olarak anti-inflamatuar aktivite gösterirken, L-NAME ve P. pungens metanol ekstresi, LPS ile uyarılan PGE2 üretimini önemli ölçüde azaltmıştır. Strach metodu sonuçlarına göre doza bağlı etki ile hücre göç hızında bir artış gözlemlenmiştir. Bulgularımız, starch testine göre P. pungens metanol ekstresinin yara iyileşmesinde rol oynayabileceğini ve antioksidan ve antiinflamatuar aktivitesinin de katkıda bulunduğu düşündürmüştür. İn vivo koşullar altında in vitro aktiviteyi doğrulamak için başka değerlendirmeler devam etmektedir

Bioautography for evaluation of several Lavandula L. and origanum species antimicrobial and antioxidant activity

In the search of bioactive natural compounds, bioautography of plant extracts were associated in an antioxidant screening. Due to containing variety of phenolic compounds Lavandula and Origanum species are important medicinal plants. The antioxidant and free radical scavenging activities of Lavandula angustifolia, L. stoechas, L. heterophylla, Origanum majorana, O. onites, O. vulgare, O. minituflorum, and their main phenolic compounds linalool and carvacrol was carried out by TLC-bioautography method based on the DPPH· andABTS·+ assays to compare essential oils and known main active constituents. The antimicrobial activity of the materials was tested using the in vitro broth microdilution assay towards two different microorganisms. Methicillin-resistant Staphylococcus aureus and Streptococcus mutans were used for the study. As a result of our studies, it is determined that O. vulgare showed the highest activity against S. mutans and O. onites and O. vulgare showed the highest activity against MRSA. compared to the tested antibiotic

Traditional herbal drugs against liver diseases – Experimented in vitro using HepG2 cells for induced steatosis

Salvia fruticosa leaves, Malva sylvestris flowers, Taraxacum officinale aerial parts, Plantago ovata seeds, Tanacetum parthenium aerial parts, and Allium sativum bulbs are documented for traditional use against hepatic disorders and different liver diseases. To evaluate herbal drug material for potential use against liver diseases, at molecular level for the efficacy linked to ethnobotanical documented data.Different herbal extracts were prepared and standardized by HPLC, according to European Pharmacopoeia. Initially 0.25 mg/mL each standardized extract was applied to oleic+palmitic acid induced fatty liver using a HepG2 cell culture model. ALT, AST, GSH, and MDA levels were comparatively analyzed, in addition to cell Nil Red staining. The highest activity for MDA reduction was observed for the A. sativum extract at 48.2% level, followed by 36.4% for M. sylvestris, and S. fruticosa extracts with 27.3% reduction, respectively. Glutathione levels increased to 59.1% when A. sativum extract was applied. M. sylvestris extract increased the glutathione levels in the medium by 49.7%; S. fruticosa extract decreased ALT levels by 53.5% and M. sylvestris extract by 38.5%, whereas the standard resveratrol reduced ALT level by 30.9%, respectively. The AST levels for M. sylvestris extract was 46.5%, compared to resveratrol by 93%. A. sativum, M. sylvestris, and S. fruticosa standard extracts showed relatively good correlation and activity where further in vivo studies should be performed

In vitro antiviral evaluations of coldmix(R): An essential oil blend against SARS-CoV-2

Coldmix® is a commercially available Eucalyptus aetheroleum and, Abies aetheroleum blend for medicinal applications. In this present study, the in vitro antiviral potential of Coldmix®, and its major constituents 1,8-cineole and α-pinene were evaluated by using the in vitro ACE2 enzyme inhibition assay as well as the direct contact test against SARS-CoV-2. The observed ACE2 enzyme inhibitory activity of Coldmix®, 1,8-cineole, and α-pinene were 72%, 88%, and 80%, respectively; whereas in the direct contact test in the vapor phase, the destruction of the virus was 79.9% within 5 min and 93.2% in the 30th min, respectively. In a similar Coldmix® vapor phase setup using the in vitro cytotoxicity cell assay, E6 VERO healthy cells were experimentally not affected by toxicity. According to the promising initial antiviral results of Coldmix® and the individually tested constituents, detailed further in vivo evaluation using different virus classes is suggested