Stump Appendicitis: An Uncompleted Surgery, a Rare but Important Entity with Potential Problems

Appendicectomy for appendicitis is one of the commonest surgical procedures performed worldwide. The residual appendiceal stump left after an initial appendectomy risks the development of stump appendicitis. Stump appendicitis is a real recognized entity but not often considered when evaluating patients with right lower quadrant abdominal pain, especially those with past history of appendectomy. It remains a clinical challenge with the result that its diagnosis and effective treatment are often delayed with possible attendant morbidity or mortality. Stump appendicitis results from obstruction of the lumen of the remaining appendix stump, usually by a faecolith. This increases intraluminal pressure, impairing venous drainage and allowing subsequent bacterial infection. We present the case of a twenty-five (25)-year-old female who underwent laparoscopic appendicectomy and presented four and half (4(1/2)) months later with fever, right lower quadrant abdominal pain, and tenderness associated with repeated vomiting. Exploratory laparotomy was carried out after clinical and imaging studies which revealed big inflammatory mass with abscess at the right iliac fossa and recurrent appendicitis of the appendiceal stump. Surgical treatment is easy but recognition of this important entity but potentially dangerous condition should always be borne in mind in order to avoid delay in its diagnosis and treatment

Performance evaluation and characterization of wetted soil parameters of improvised medi-emitters installed in a drip irrigation tomato field

Field study was conducted to evaluate the emission uniformity (EU), global coefficient of variation (CGv), emitter flow variation (Qvar) and distribution uniformity (DU), and determine the wetted radius (rw) on soil surface of improvised medi-emitters installed in a tomato field. Soil water content (SWC) at four layers was determined after different periods of irrigation. Radius of wetted soil surface was determined and predicted. Irrigation frequency had no significant effect on the average discharge rate of the medi-emitters throughout the growing cycle. Average Qvar and CGv were significantly (P=0.05) influenced by the frequency of application while the EU and DU did not significantly (P=0.05) differ among the treatments. There were significant differences in the average values of SWC in different soil layers under the different periods of irrigation. Both the observed and calculated rw on the soil surface were fitted with fourth order polynomial. The model performance parameters of MAE and RMSE between the calculated and observed radii were low, indicating good prediction. Medical infusion set can successfully replace the more expensive conventional emitters for drip irrigation system

Nutritionally Improved Cookies from Composite Flour: African Walnut (Tetracarpidium conophorum) with Wheat

African walnut seed (Tetracarpidium conophorum) is rich in protein and phytochemical with great potentials for food application, but has limited uses in food industry. The African walnut seed was procured from Oje market and commercial wheat flour in Aleshiloye market, Ibadan. The commercial wheat and African walnut flours were composites of varying ratios; 100:0, 90:10, 80:20, 70:30 and 60:40 to prepare cookies and labeled samples X, A, B, C and D respectively. The proximate composition, anti-nutrients (oxalates, phytate and protease inhibitor), phyto-chemical (tannins, flavonoids, alkaloids, terpenoids, saponins, and Oxygen Radical Absorbance Capacity (ORAC) and sensory evaluation of the cookies were determined using standard methods. The result of proximate composition of cookies showed that carbohydrate (47.0-52.36%), protein (16.3-19.6%) and fat (18.5-19.2%) were the major components in the cookie samples. Other components including; moisture (7.7-8.4%), ash (4.1-4.2%) and fibre (0.9-1.8%) were generally low. Anti-nutrient factors of cookie samples ranged from 11.3-17.7mg/100g for Phytate and no traces of oxalates and protease inhibitors in all cookie samples. Control cookie sample had no traces of tannin, flavonoids and saponins, but enriched cookie ranged from 10.3-14.7mg/100g; 3.4-5.7mg/100g and 10.7-15.3mg/100g, respectively. Phytochemical constituents of cookie samples in ORAC ranged from 3.4-12.0mg/100g and alkaloids (3.7-7.7mg/100g). Organoleptic panelists preferred sample X to all other samples, followed by sample A. Inclusion of 10% African walnut flour compared favorably with wheat cookie in terms of sensory quality

Assessment of Microplastics Contamination in River Water, Bottled Water, Sachet Water and Branded Table Salt Samples in Kaduna Metropolis, Nigeria

Microplastics (MPs) < 5 mm-sized are regarded as global environmental contaminants. This study analyzes MP concentrations in the Kaduna River (raw water), treated water from two conventional water treatment plants, brands of bottled water, and food-grade salts available in Kaduna Metropolis, Nigeria using standard methods. Data obtained show that levels of MPs ranged from 25 to 36 particles L-1 in treated water, and to 153 particles L-1 in raw water. While samples of bottled water contained 1.4 to 3.7 particles L-1 and samples of table salt contained 0.13 to 0.27 particles g-1. Water and salt samples contained five different types of polymers, including polyethylene, polypropylene, polyester, polyvinyl chloride and polyethylene terephthalate. Additionally, MPs were divided into three groups based on their physical characteristics. In both raw and treated water, fragments were clearly more prevalent; in samples of bottled water and table salt, fragments and fibers predominated. Microplastics in bottled water pose a medium pollution risk, according to pollution risk indices. The estimated daily intake was generally minimal, indicating little harm from daily consumption, but it also demonstrates that children have a larger intake of microplastics than adults. Leaching from the packing material was identified as the MPs' primary source. This study fills the knowledge gap in the area of emerging microplastic pollution of water sources, drinking water, and food-grade salt

Investigating the functionality of an OCT4-short response element in human induced pluripotent stem cells.

Pluripotent stem cells offer great therapeutic promise for personalized treatment platforms for numerous injuries, disorders, and diseases. Octamer-binding transcription factor 4 (OCT4) is a key regulatory gene maintaining pluripotency and self-renewal of mammalian cells. With site-specific integration for gene correction in cellular therapeutics, use of the OCT4 promoter may have advantages when expressing a suicide gene if pluripotency remains. However, the human OCT4 promoter region is 4 kb in size, limiting the capacity of therapeutic genes and other regulatory components for viral vectors, and decreasing the efficiency of homologous recombination. The purpose of this investigation was to characterize the functionality of a novel 967bp OCT4-short response element during pluripotency and to examine the OCT4 titer-dependent response during differentiation to human derivatives not expressing OCT4. Our findings demonstrate that the OCT4-short response element is active in pluripotency and this activity is in high correlation with transgene expression in vitro, and the OCT4-short response element is inactivated when pluripotent cells differentiate. These studies demonstrate that this shortened OCT4 regulatory element is functional and may be useful as part of an optimized safety component in a site-specific gene transferring system that could be used as an efficient and clinically applicable safety platform for gene transfer in cellular therapeutics

Dragon's Paradise Lost: Palaeobiogeography, Evolution and Extinction of the Largest-Ever Terrestrial Lizards (Varanidae)

BACKGROUND: The largest living lizard species, Varanus komodoensis Ouwens 1912, is vulnerable to extinction, being restricted to a few isolated islands in eastern Indonesia, between Java and Australia, where it is the dominant terrestrial carnivore. Understanding how large-bodied varanids responded to past environmental change underpins long-term management of V. komodoensis populations. METHODOLOGY/PRINCIPAL FINDINGS: We reconstruct the palaeobiogeography of Neogene giant varanids and identify a new (unnamed) species from the island of Timor. Our data reject the long-held perception that V. komodoensis became a giant because of insular evolution or as a specialist hunter of pygmy Stegodon. Phyletic giantism, coupled with a westward dispersal from mainland Australia, provides the most parsimonious explanation for the palaeodistribution of V. komodoensis and the newly identified species of giant varanid from Timor. Pliocene giant varanid fossils from Australia are morphologically referable to V. komodoensis suggesting an ultimate origin for V. komodoensis on mainland Australia (>3.8 million years ago). Varanus komodoensis body size has remained stable over the last 900,000 years (ka) on Flores, a time marked by major faunal turnovers, extinction of the island's megafauna, the arrival of early hominids by 880 ka, co-existence with Homo floresiensis, and the arrival of modern humans by 10 ka. Within the last 2000 years their populations have contracted severely. CONCLUSIONS/SIGNIFICANCE: Giant varanids were once a ubiquitous part of Subcontinental Eurasian and Australasian faunas during the Neogene. Extinction played a pivotal role in the reduction of their ranges and diversity throughout the late Quaternary, leaving only V. komodoensis as an isolated long-term survivor. The events over the last two millennia now threaten its future survival

EVALUATION OF ANALGESIC, ANTICONVULSANT AND HYPNOTIC ACTIVITIES OF PYRENACANTHIA STAUNDTII

An Aqueous leaf extract of Pyrenacanthia Staundtii (AqPs) was studied for central nervous activities. The extract (100.0 – 400.0 mg/kg i. p). significantly (