The effect of various carbon sources on the growth of single-celled cyanophyta

In 19 strains of unicellular blue-green algae, belonging to general Synechococcus, Synechocystis, Aphanocapsa and Aphanothece, the capacity of growth under mixotrophic conditions in mineral media with organic carbon sources (carbohydrates, polyols) was investigated. At moderate light intensity (1200 lx) and 0.5% of carbon source there was revealed: (1) Stimulation of growth; (2) Partial or complete inhibition of growth; (3) No influence of carbohydrate and polyols on the growth of some algae strains. Three physiological groups for the investigated strains have been outlined on the basis of data obtained. The possibility of using the differences revealed in classification of unicellular blue-green algae is discussed

Actin assembly ruptures the nuclear envelope by prying the lamina away from nuclear pores and nuclear membranes in starfish oocytes.

The nucleus of oocytes (germinal vesicle) is unusually large and its nuclear envelope (NE) is densely packed with nuclear pore complexes (NPCs) stockpiled for embryonic development. We showed that breakdown of this specialized NE is mediated by an Arp2/3-nucleated F-actin 'shell' in starfish oocytes, in contrast to microtubule-driven tearing in mammalian fibroblasts. Here, we address the mechanism of F-actin-driven NE rupture by correlated live-cell, super-resolution and electron microscopy. We show that actin is nucleated within the lamina sprouting filopodia-like spikes towards the nuclear membranes. These F-actin spikes protrude pore-free nuclear membranes, whereas the adjoining membrane stretches accumulate NPCs associated with the still-intact lamina. Packed NPCs sort into a distinct membrane network, while breaks appear in ER-like, pore-free regions. Thereby, we reveal a new function for actin-mediated membrane shaping in nuclear rupture that is likely to have implications in other contexts such as nuclear rupture observed in cancer cells

Rolling current collector in direct current machines

The new method of rolling current collector has been developed. Its theoretical research was carried out. The operating model sample of the current collecting device using the rolling contact was made

Finite element modeling of an alternating current electromagnetic weld pool support in full penetration laser beam welding of thick duplex stainless steel plates

This article may be downloaded for personal use only. Any other use requires prior permission of the author and AIP Publishing. This article appeared in M. Bachmann et al., J. Laser Appl. 28, 022404 (2016) and may be found at https://doi.org/10.2351/1.4943906.An electromagnetic weld pool support system for 20 mm thick duplex stainless steel AISI 2205 was investigated numerically and compared to experiments. In our former publications, it was shown how an alternating current (AC) magnetic field below the process zone directed perpendicular to the welding direction can induce vertically directed Lorentz forces. These can counteract the gravitational forces and allow for a suppression of material drop-out for austenitic stainless steels and aluminum alloys. In this investigation, we additionally adopted a steady-state complex magnetic permeability model for the consideration of the magnetic hysteresis behavior due to the ferritic characteristics of the material. The model was calibrated against the Jiles–Atherton model. The material model was also successfully tested against an experimental configuration before welding with a 30 mm diameter cylinder of austenitic stainless steel surrounded by duplex stainless steel. Thereby, the effects of the Curie temperature on the magnetic characteristics in the vicinity of the later welding zone were simulated. The welding process was modeled with a three-dimensional turbulent steady-state model including heat transfer and fluid dynamics as well as the electromagnetic field equations. Main physical effects, the thermo-capillary (Marangoni) convection at the weld pool boundaries, the natural convection due to gravity as well as latent heat of solid–liquid phase transitions at the phase boundaries were accounted for in the model. The feedback of the electromagnetic forces on the weld pool was described in terms of the electromagnetic-induced pressure. The finite element software COMSOL Multiphysics 4.2 was used in this investigation. It is shown that the gravity drop-out associated with the welding of 20 mm thick duplex stainless steel plates due to the hydrostatic pressure can be prevented by the application of AC magnetic fields between around 70 and 90 mT. The corresponding oscillation frequencies were between 1 and 10 kHz and the electromagnetic AC powers were between 1 and 2.3 kW. In the experiments, values of the electromagnetic AC power between 1.6 and 2.4 kW at oscillation frequencies between 1.2 and 2.5 kHz were found to be optimal to avoid melt sagging or drop-out of melt in single pass full-penetration laser beam welding of 15 and 20 mm thick AISI 2205

HUBUNGAN TINGKAT PENDIDIKAN KEPALA KELUARGA DENGAN TINGKAT KESIAPSIAGAAN BENCANA TSUNAMI DI DESA ALUE NAGA KECAMATAN SYIAH KUALA KOTA BANDA ACEH

ABSTRAKTsunami pada Desember 2004, yang dipicu oleh gempa berkekuatan 9.0 SR di sebelah utara pulau Sumatra mengakibatkan kerugian yang sangat besar. Aceh merupakan daerah paling parah dengan korban tewas sebanyak 123.000 jiwa, 113.000 orang hilang, 406.000 orang kehilangan tempat tinggal. Faktor utama timbulnya banyak korban akibat bencana gempa bumi adalah kurangnya pengetahuan masyarakat tentang bencana dan kesiapan mereka dalam mengantisipasi bencana. Sekolah merupakan salah satu media transformasi ilmu pengetahuan yang paling efektif dalam menyerap dan mengaplikasikan pengetahuan kesiapan menghadapi bencana dengan menggunakan metode yang tepat dan benar. Tujuan penelitian ini adalah untuk mengetahui apakah ada hubungan tingkat pendidikan formal dengan tingkat kesiapsiagaan dalam menghadapi bencana tsunami. Populasi penelitian ini 494 orang kepala keluarga dan terdapat jumlah sampel sebanyak 84 responden. Pengambilan sampel yang digunakan adalah non-probability sampling. Pengumpulan data menggunakan angket dengan wawancara terarah dan diolah dengan menggunakan statistik uji Kolmogorov Smirnov dengan hasil 55 responden (66%) memiliki kesiapsiagaaan rendah dan tingkat pendidikan yang paling banyak adalah pendidikan dasar berjumlah 67 responden (80%). Kesimpulan dalam penelitian ini adalah terdapat pengaruh tingkat pendidikan dengan tingkat kesiapsiagaan kepala keluaga di Desa Alue Naga kecamatan Syiah Kuala Kota Banda Aceh dengan nilai p=0,000 (pBanda Ace

Numerical assessment and experimental verification of the influence of the Hartmann effect in laser beam welding processes by steady magnetic fields

Controlling the dynamics in the weld pool is a highly demanding challenge in deep-penetration laser beam welding with modern high power laser systems in the multi kilowatt range. An approach to insert braking forces in the melt which is successfully used in large-scaled industrial applications like casting is the so-called Hartmann effect due to externally applied magnetic fields. Therefore, this study deals with its adaptation to a laser beam welding process of much smaller geometric and time scale. In this paper, the contactless mitigation of fluid dynamic processes in the melt by steady magnetic fields was investigated by numerical simulation for partial penetration welding of aluminium. Three-dimensional heat transfer, fluid dynamics including phase transition and electromagnetic field partial differential equations were solved based on temperature-dependent material properties up to evaporation temperature for two different penetration depths of the laser beam. The Marangoni convection in the surface region of the weld pool and the natural convection due to the gravitational forces were identified as main driving forces in the weld pool. Furthermore, the latent heat of solid–liquid phase transition was taken into account and the solidification was modelled by the Carman–Kozeny equation for porous medium morphology. The results show that a characteristic change of the flow pattern in the melt can be achieved by the applied steady magnetic fields depending on the ratio of magnetic induced and viscous drag. Consequently, the weld bead geometry was significantly influenced by the developing Lorentz forces. Welding experiments with a 16 kW disc laser with an applied magnetic flux density of around 500 mT support the numerical results by showing a dissipating effect on the weld pool dynamics