Potomac River pound-net survey, summers 1996-1997 : 1998 final report

The pound net (Fig 1) is a fixed fishing structure that intercepts fish as they migrate up- or down-river. Fish weirs made of stakes were first used by the Native Americans along the east coast of the English Colonies and were the model for the later development of the pound net. Pound nets were first used in New England around 1850; and were subsequently introduced to Long Island in 1855. A Captain Henry Fitzgerald is reported to have erected the first Chesapeake Bay pound net in 1858, but it failed due to its poor construction. No further attempts were made to use this gear until 1870. At this time two New Jersey fishermen successfully fished a pound net in the James River. A second net was erected in 1875 in the Mob jack Bay, and was so successful that the local fishermen destroyed it. The design of the net was passed around among the fishermen and by 1876 there were 12 nets. So great was the profit that by 1880 there were 162 nets in Virginia (Reid 1955, Austin 1987). The design of the gear has changed little over the years; and during the period between the World Wars the number of nets in the Chesapeake Bay exceeded 2,000. Since the mid-1950\u27s the numbers have declined. June (1956) cited competition with the otter trawl after the mid-1950\u27s as one reason for a general decline in pound nets in the Mid-Atlantic Bight states. (...

Metabolome-Informed Microbiome Analysis Refines Metadata Classifications and Reveals Unexpected Medication Transfer in Captive Cheetahs.

Even high-quality collection and reporting of study metadata in microbiome studies can lead to various forms of inadvertently missing or mischaracterized information that can alter the interpretation or outcome of the studies, especially with nonmodel organisms. Metabolomic profiling of fecal microbiome samples can provide empirical insight into unanticipated confounding factors that are not possible to obtain even from detailed care records. We illustrate this point using data from cheetahs from the San Diego Zoo Safari Park. The metabolomic characterization indicated that one cheetah had to be moved from the non-antibiotic-exposed group to the antibiotic-exposed group. The detection of the antibiotic in this second cheetah was likely due to grooming interactions with the cheetah that was administered antibiotics. Similarly, because transit time for stool is variable, fecal samples within the first few days of antibiotic prescription do not all contain detected antibiotics, and the microbiome is not yet affected. These insights significantly altered the way the samples were grouped for analysis (antibiotic versus no antibiotic) and the subsequent understanding of the effect of the antibiotics on the cheetah microbiome. Metabolomics also revealed information about numerous other medications and provided unexpected dietary insights that in turn improved our understanding of the molecular patterns on the impact on the community microbial structure. These results suggest that untargeted metabolomic data provide empirical evidence to correct records and aid in the monitoring of the health of nonmodel organisms in captivity, although we also expect that these methods may be appropriate for other social animals, such as cats.IMPORTANCE Metabolome-informed analyses can enhance omics studies by enabling the correct partitioning of samples by identifying hidden confounders inadvertently misrepresented or omitted from carefully curated metadata. We demonstrate here the utility of metabolomics in a study characterizing the microbiome associated with liver disease in cheetahs. Metabolome-informed reinterpretation of metagenome and metabolome profiles factored in an unexpected transfer of antibiotics, preventing misinterpretation of the data. Our work suggests that untargeted metabolomics can be used to verify, augment, and correct sample metadata to support improved grouping of sample data for microbiome analyses, here for nonmodel organisms in captivity. However, the techniques also suggest a path forward for correcting clinical information in microbiome studies more broadly to enable higher-precision analyses

Modeling peer effect modification by network strength: The diffusion of implantable cardioverter defibrillators in the US hospital network

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/154422/1/sim8466.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/154422/2/sim8466_am.pd

Test, Trace, and Isolate: Covid-19 and the Canadian Constitution

Contact tracing is essential to controlling the spread of infectious disease and plays a central role in plans to safely loosen Covid-19 physical distancing measures and begin to reopen the economy. Contact tracing apps, used in conjunction with established human contact tracing methods, could serve as part of Canada’s “test, trace, and isolate” strategy. In this brief, we consider the potential benefits of using contract tracing apps to identify people who have been exposed to Covid-19, as well as the limitations of using this technology. We also consider the privacy implications of different app design choices. Finally, we consider how the privacy impacts of contact tracing apps could be evaluated under the Canadian Charter of Rights and Freedoms, which provides a framework for balancing competing rights and interests. We argue that so long as apps are carefully constructed and the information they reveal is appropriately safeguarded, tracing apps may have a role to play in the response of a free and democratic society to the Covid 19 pandemic. 1. Improving the Efficiency of Human Contact Tracing: The public health goal of a contact tracing app should be to integrate with human contact tracing and make it more efficient rather than replace it. We need to keep humans in the loop to ensure accuracy and to maintain the important social functions of contact tracing, which includes educating people about risks and helping them access social supports. 2. Privacy Choices: Currently, the most privacy-protective design for contact tracing apps makes use of proximity data (via Bluetooth) through a decentralized design. This method is receiving significant technical support from Apple and Google. However, this method fails to integrate with the human contact tracing system. Other options, such as the use of location logs or a centralized registration system, are more aligned with the public health goal of integration with human contact tracing but raise additional privacy questions. In addition to the constitutional questions raised by these privacy choices, there are two important considerations. First, social trust is important. If individuals do not feel comfortable with using a particular contact tracing app there will not be the large-scale uptake needed to make these an effective addition to human contact tracing. Second, due to various technical challenges, it is difficult to make effective contact tracing apps utilizing proximity data unless one uses the method supported by Apple and Google. However, Google and Apple prohibit app developers both from utilizing centralized methods and from utilizing location data. 3. Constitutional Balancing: Our privacy commissioners have discussed the need to assess these privacy choices according to the principles of necessity and proportionality. The Canadian Charter provides an important framework for thinking about these principles as it provides us with a framework for how to balance rights and interests in a free and democratic society. The Charter requires that we choose the most privacy-protective app design that meets the public health goal, so long as the benefits of meeting this goal outweigh its deleterious effects on privacy. This requires a reasonable belief in the efficacy of such an app. It also requires an assessment of the nature of the benefits, which are not just the economic benefits of reopening the economy. The currently prevailing restrictions on movement and work are themselves limitations of basic rights and liberties. Individuals who self-isolate in situations of poverty, precarious housing, mental health challenges, abusive relationships, or other vulnerabilities face challenges that affect their security of the person. There are also broader effects on equality and human flourishing. If contact tracing, enhanced by an app, reduces the need for restrictions in the form of self-isolation, it promotes other Charter rights and values (e.g., security of the person) which must be balanced against the potential infringement of privacy rights

Encontro casual na região central do Texas fornece informações sobre a ecologia da estivação de Siren nettingi (Caudata: Sirenidae)

Siren spp. costumam ser vertebrados dominantes nas áreas úmidas que ocupam e são conhecidas por estivar quando essas áreas úmidas secam. Considerações práticas limitam as observações in situ de indivíduos em estivação. Em 12 de outubro de 2021, descobrimos por acaso um agregado em estivação de Siren nettingi no condado de Bastrop, Texas, Estados Unidos. Essas salamandras foram escavadas em solo compacto e rochoso adjacente a uma estrada de caliche, em profundidades que variavam entre ~0,2 e 1,5 m. A vegetação dominante nesse local incluía Ulmus crassifolia, Persicaria sp. e várias espécies de gramíneas. Recuperamos 140 indivíduos, dos quais sete foram resgatados e 133 foram capturados vivos. Medimos 115 deles quanto ao comprimento rostro-cloacal (SVL) e observamos que o agregado era dominado por jovens. Estimamos uma densidade de estivação de 2,33 indivíduos/m2 que é comparável às densidades estimadas para populações sem estivação. No entanto, como não houve monitoramento para esse estudo, provavelmente tenha ocorrido um evento de mortalidade em massa. Portanto, sugerimos que a construção de estradas no habitat preferido seja considerada uma ameaça às populações dessas salamandras.Siren spp. are often dominant vertebrates in the wetlands they occupy and are known to estivate when such wetlands dry up. Practical considerationslimit in-situ observations of estivating individuals. On 12 October 2021, we incidentally discovered an estivating aggregate of Siren nettingi in Bastrop County, Texas, USA. These salamanders were excavated from compact, rocky soil adjacent to a caliche road, at depths that ranged between ~0.2 to 1.5 m. The dominant vegetation at this site included Ulmus crassifolia, Persicaria sp., and various grass species. We recovered 140 individuals of which seven were salvaged and 133 were captured live. We measured 115 of these for snout–vent length (SVL) and observed the aggregate was predominated by juveniles. We estimated an estivation density of 2.33 sirens/m2 that is comparable to densities estimated for non-estivating populations. However, in-lieu of monitoring that was in place for this study, we expect a mass mortality event would have likely occurred. We therefore suggest that roadway construction in preferred habitat be considered as a threat to siren populations

Association between diagnosis code expansion and changes in 30-day risk-adjusted outcomes for cardiovascular diseases

BACKGROUND In January 2011, Centers for Medicare and Medicaid Services expanded the number of inpatient diagnosis codes from 9 to 25, which may influence comorbidity counts and risk-adjusted outcome rates for studies spanning January 2011. This study examines the association between (1) limiting versus not limiting diagnosis codes after 2011, (2) using inpatient-only versus inpatient and outpatient data, and (3) using logistic regression versus the Centers for Medicare and Medicaid Services risk-standardized methodology and changes in risk-adjusted outcomes. METHODS AND RESULTS Using 100% Medicare inpatient and outpatient files between January 2009 and December 2013, we created 2 cohorts of fee-for-service beneficiaries aged ≥65 years. The acute myocardial infarction cohort and the heart failure cohort had 578 728 and 1 595 069 hospitalizations, respectively. We calculate comorbidities using (1) inpatient-only limited diagnoses, (2) inpatient-only unlimited diagnoses, (3) inpatient and outpatient limited diagnoses, and (4) inpatient and outpatient unlimited diagnoses. Across both cohorts

Understanding Assistive Programs: Evaluating the Impact of Various Math Services on Student Performance

High school math performance has the potential to have a positive impact on later educational success. This project seeks to determine the role of math services and intervention programs including online and in-person tutoring, workshops, coaching, physical tools, and other class services on students’ performance in the North Carolina’s High School Math 1 course. Data being used was gathered from rural North Carolina middle and high schools by the college access program GEAR UP (Gaining Early Awareness and Readiness for Undergraduate Programs). The effects of these services will be measured by End of Grade (EOG) test and course grades in the Math 1 course. This project will help develop a deeper understanding of how these types of intervention services impact students\u27 performance in current and future math courses

1-800-Help-Me-With-Open-Science-Stuff: A Qualitative Examination of Open Science Practices in Communication Sciences and Disorders

Purpose: The purpose of this qualitative study was to examine the perceptions of communication sciences and disorders (CSD) assistant professors in the United States related to barriers and facilitators to engaging in open science (OS) practices and identify opportunities for improving OS training and support in the field. Method: Thirty-five assistant professors (16 from very high research activity (R1) institutions, 19 from institutions with other Carnegie classifications) participated in one 1-hour virtual focus group conducted via Zoom recording technology. The researchers used a conventional content analysis approach to analyze the focus group data and develop categories from the discussions. Results: Five categories were developed from the focus groups: (a) a desire to learn about OS through opportunities for independent learning and learning with peers, (b) perceived benefits of engaging in OS on assistant professors’ careers, the broader scientific community, and the quality of research in the field of CSD; (c) personal factors that act as barriers and/or facilitators to engaging in OS practices; (d) systemic factors that act as barriers and/or facilitators to engaging in OS practices; and (e) differences in perceptions of R1 and non-R1 assistant professors. Conclusions: Assistant professors in CSD perceive benefits of OS for their careers, the scientific community, and the field. However, they face many barriers (e.g., time, lack of knowledge and training) which impede their engagement in OS practices. Recommendations for CSD assistant professors, academic institutions, publishers, and funding agencies are provided to reduce barriers to engagement in OS practices

The Biarzo case in northern Italy: Is the temporal dynamic of swine mitochondrial DNA lineages in Europe related to domestication?

Genetically-based reconstructions of the history of pig domestication in Europe are based on two major pillars: 1) the temporal changes of mitochondrial DNA lineages are related to domestication; 2) Near Eastern haplotypes which appeared and then disappeared in some sites across Europe are genetic markers of the first Near Eastern domestic pigs. We typed a small but informative fragment of the mitochondrial DNA in 23 Sus scrofa samples from a site in north eastern Italy (Biarzo shelter) which provides a continuous record across a ≈6,000 year time frame from the Upper Palaeolithic to the Neolithic. We additionally carried out several radiocarbon dating. We found that a rapid mitochondrial DNA turnover occurred during the Mesolithic, suggesting that substantial changes in the composition of pig mitochondrial lineages can occur naturally across few millennia independently of domestication processes. Moreover, so-called Near Eastern haplotypes were present here at least two millennia before the arrival of Neolithic package in the same area. Consequently, we recommend a re-evaluation of the previous idea that Neolithic farmers introduced pigs domesticated in the Near East, and that Mesolithic communities acquired domestic pigs via cultural exchanges, to include the possibility of a more parsimonious hypothesis of local domestication in Europe

Bi-cultural dynamics for risk and protective factors for cardiometabolic health in an Alaska Native (Yup\u27ik) population

Alaska Native people experience disparities in mortality from heart disease and stroke. This work attempts to better understand the relationships between socioeconomic, behavioral, and cardiometabolic risk factors among Yup\u27ik people of southwestern Alaska, with a focus on the role of the socioeconomic, and cultural components. Using a cross-sectional sample of 486 Yup\u27ik adults, we fitted a Partial Least Squares Path Model (PLS-PM) to assess the associations between components, including demographic factors [age and gender], socioeconomic factors [education, economic status, Yup\u27ik culture, and Western culture], behavioral factors [diet, cigarette smoking and smokeless tobacco use, and physical activity], and cardiometabolic risk factors [adiposity, triglyceride-HDL and LDL lipids, glycemia, and blood pressure]. We found relatively mild associations of education and economic status with cardiometabolic risk factors, in contrast with studies in other populations. The socioeconomic factor and participation in Yup\u27ik culture had potentially protective associations with adiposity, triglyceride-HDL lipids, and blood pressure, whereas participation in Western culture had a protective association with blood pressure. We also found a moderating effect of participation in Western culture on the relationships between Yup\u27ik culture participation and both blood pressure and LDL lipids, indicating a potentially beneficial additional effect of biculturalism. Our results suggest that reinforcing protective effects of both Yup\u27ik and Western cultures could be useful for interventions aimed at reducing cardiometabolic health disparities