Nerve growth factor, mast cells and arthritis

Nerve growth factor is a well-characterized neurotrophic protein required for the survival and differentiation of a variety of neuronal cell types both in the peripheral and central nervous systems. Recent studies indicate that nerve growth factor also plays a role in cells originating in the immune system, since it is synthesized by cells of immune system lineage and its level increases during inflammatory responses. Moreover, it has been shown that cytokines such as interleukin 1β and tumor necrosis factor-α are potent inducers of nerve growth factor secretion. These studies were recently confirmed and extended by demonstrating that cells normally present in inflammatory tissues, such as mast cells and lymphocytes, express nerve growth factor receptors and are receptive to the action of nerve growth factor. The aim of the present review is to outline the current understanding of mast cells and nerve growth factor in autoimmune diseases and particularly in arthritis.Biomedical Reviews 1995; 4: 7-14

Hypercomplex Image-to-Image Translation

Image-to-image translation (I2I) aims at transferring the content representation from an input domain to an output one, bouncing along different target domains. Recent I2I generative models, which gain outstanding results in this task, comprise a set of diverse deep networks each with tens of million parameters. Moreover, images are usually three-dimensional being composed of RGB channels and common neural models do not take dimensions correlation into account, losing beneficial information. In this paper, we propose to leverage hypercomplex algebra properties to define lightweight I2I generative models capable of preserving pre-existing relations among image dimensions, thus exploiting additional input information. On manifold I2I benchmarks, we show how the proposed Quaternion StarGANv2 and parameterized hypercomplex StarGANv2 (PHStarGANv2) reduce parameters and storage memory amount while ensuring high domain translation performance and good image quality as measured by FID and LPIPS scores. Full code is available at: https://github.com/ispamm/HI2I

Tape winding angle influence on subsea cable sheathing fatigue performance

A fundamental component of subsea power cables is the thin galvanized steel tape winded around the dielectric and sheathing layer in order to prevent permanent thermal cycling induced deformation. The pressure state induced by the resistance offered by such tapes against radial reformation reduces the triaxiality ratio of the stress state of the lead sheathing layer. It is known that a reduced triaxiality has a beneficial effect on ductility and fatigue life of metals. In the present work a series of finite element simulations are performed in presence of galvanized steel tapes at three different winding angles and without such reinforcement at all, obtaining a qualitative indication of its effect on the stress state induced in the sheathing layer. Loading conditions as internal pressure related to thermal dielectric expansion and cable bending are modelled. The numerical qualitative results are discussed in connection to a series of full-scale fatigue tests performed on subsea power cables with and without the support of steel tapes. 2020 The Author(s). Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/

Alfabetizzazione digitale, scrittura enciclopedica ed educazione linguistica democratica

The text describes two different kinds of literacy: the traditional and the digital one. It then places them in a context of “Linguistic education” (educazione linguistica), highlighting the possible benefits of a more significant inclusion of real practices within the educational curricula. This is done by looking at both “vernacular literacy” practices and practices taken from the workplace. The use of Wikipedia in the Italian language classroom is afterwards introduced as an example of such possible integrations

Vaginal Evisceration of Small Bowel With Extraperitoneal Ileal Resection of the Herniated Loops: A Case Report

Introduction: Vaginal evisceration is an extremely rare surgical emergency that can be described as the extrusion of abdominal viscera through a defect or a rupture of the vaginal wall. We reported the case of an acute abdomen due to small bowel evisceration secondary to vaginal vault dehiscence that required combined vaginal-abdominal approach Case: We discuss the case of a 72-year-old female who presented to the emergency department for a large prolapse with visible extrusion of the small bowel per vagina. The eviscerated bowel was resected by external vaginal approach due to excessive swelling of the loops which made it impossible to reduce them through the vagina defect. A midline laparotomy was undertaken for further assessment, and the vault defect was closed by transabdominal repair Conclusion: From its first description in 1864, just a few cases of vaginal evisceration had been described in the medical literature; the most common organ to eviscerate is the distal ileum, although cases of omentum, colon, fallopian tube, and appendix evisceration have also been reported. We described a rare case of transvaginal evisceration of the small bowel in our emergency department; it is a rare surgical emergency that must be managed to prevent serious consequences, such as bowel ischemia and necrosis, sepsis, and death. We suggest that a multidisciplinary approach to prompt examination and management by gynecologists and general surgeons is recommended to reduce the risk of morbidity and mortality. With this paper the authors would like to share the surgical manage of this rare emergency with other surgeons all around the world

Pulse EPR-enabled interpretation of scarce pseudocontact shifts induced by lanthanide binding tags

Pseudocontact shifts (PCS) induced by tags loaded with paramagnetic lanthanide ions provide powerful long-range structure information, provided the location of the metal ion relative to the target protein is known. Usually, the metal position is determined by fitting the magnetic susceptibility anisotropy (Δχ) tensor to the 3D structure of the protein in an 8-parameter fit, which requires a large set of PCSs to be reliable. In an alternative approach, we used multiple Gd(3+)-Gd(3+) distances measured by double electron-electron resonance (DEER) experiments to define the metal position, allowing Δχ-tensor determinations from more robust 5-parameter fits that can be performed with a relatively sparse set of PCSs. Using this approach with the 32 kDa E. coli aspartate/glutamate binding protein (DEBP), we demonstrate a structural transition between substrate-bound and substrate-free DEBP, supported by PCSs generated by C3-Tm(3+) and C3-Tb(3+) tags attached to a genetically encoded p-azidophenylalanine residue. The significance of small PCSs was magnified by considering the difference between the chemical shifts measured with Tb(3+) and Tm(3+) rather than involving a diamagnetic reference. The integrative sparse data approach developed in this work makes poorly soluble proteins of limited stability amenable to structural studies in solution, without having to rely on cysteine mutations for tag attachment.Financial support by the Australian Research Council (ARC) and an Australia-Weizmann Making Connections grant is gratefully acknowledged. B. G. thanks the ARC for a Future Fellowship

Cannabis sativa L. in Foodstuffs: the Italian Case and the Need for EU Harmonised Limits for THC Unavoidable Contamination

The following report aims at a short description of the plant species Cannabis sativa L. from a scientific perspective, in order to provide key aspects related to botanical classification, secondarymetabolism, phytochemistry and breeding; highlighting the legal uncertainties linked to the lack of harmonized regulatory frameworks at EU level for hemp and cannabis-derived foods

Delineating the Mode of Action of Adenosine A 1 Receptor Allosteric Modulators □ S

ABSTRACT Despite the identification of 2-amino-3-benzoylthiophenes (2A3BTs) as the first example of small-molecule allosteric potentiators of agonist function at a G protein-coupled receptor (GPCR)-the adenosine A 1 receptor-their mechanism of action is still not fully understood. We now report the mechanistic basis for the complex behaviors noted for 2A3BTs at A 1 receptors. Using a combination of membranebased and intact-cell radioligand binding, multiple signaling assays, and a native tissue bioassay, we found that the allosteric interaction between 2A3BTs and the agonists 2-chloro- 3 H]8-cyclopentyl-1,3-dipropylxanthine is consistent with a ternary complex model involving recognition of a single extracellular allosteric site. However, when allowed access to the intracellular milieu, 2A3BTs have a secondary action as direct G protein inhibitors; this latter property is receptor-independent as it is observed in nontransfected cells and also after stimulation of another GPCR. In addition, we found that 2A3BTs can signal as allosteric agonists in their own right but show bias toward certain pathways relative to the orthosteric agonist, R-PIA. These results indicate that 2A3BTs have a dual mode of action when interacting with the A 1 receptor and that they can engender novel functional selectivity in A 1 signaling. These mechanisms need to be factored into allosteric ligand structure-activity studies