Treatment of grade III furcation lesion by tunneling in a smoker patient: Clinical Case Report

The aim of this study is to describe a case report of lower molar treatment with grade III furcation by the tunneling technique in a heavy smoker patient. Case presentation: In the present study, a 40-year- -old man, a heavy smoker (> 20 cigarettes / day) presented a grade III furcation injury on dental element 46 after a clinical and radiographic examination. After non-surgical periodontal therapy, the patient was treated with the execution of the tunneling of the furcation of the tooth 46, which was effective in the control of the disease and in the maintenance of the dental element after 12 months of follow up. Final considerations: The tunneling technique was effective in the control of periodontal disease in a heavy smoking patient, with a good standard of hygiene and motivation, and good clinical results were maintained after 12 months of follow-up

Characterization of ligature‐induced experimental periodontitis

We sought to better characterize the progression of periodontal tissue breakdown in rats induced by a ligature model of experimental periodontal disease (PD). A total of 60 male Sprague–Dawley rats were evenly divided into an untreated control group and a PD group induced by ligature bilaterally around first and second maxillary molars. Animals were sacrificed at 1, 3, 5, 7, 14, and 21 days after the induction of PD. Alveolar bone loss was evaluated by histomorphometry and microcomputed tomography (μCT). The immune‐inflammatory process in the periodontal tissue was assessed using descriptive histologic analysis and quantitative polymerase chain reaction (qPCR). This ligature model resulted in significant alveolar bone loss and increased inflammatory process of the periodontal tissues during the initial periods of evaluation (0–14 days). A significant increase in the gene expression of pro‐inflammatory cytokines, interleukin‐1β (IL‐1β), interleukin‐6 (IL‐6), and tumor necrosis factor‐α (TNF‐α), and proteins involved in osteoclastogenesis, receptor activator of nuclear factor‐k B ligand (RANKL) and osteoprotegerin (OPG) was observed in the first week of analysis. In the later periods of evaluation (14–21 days), no significant alterations were noted with regard to inflammatory processes, bone resorption, and expression of cytokine genes. The ligature‐induced PD model resulted in progressive alveolar bone resorption with two different phases: Acute (0–14 days), characterized by inflammation and rapid bone resorption, and chronic (14–21 days) with no significant progression of bone loss. Furthermore, the gene expressions of IL‐6, IL‐1β, TNF‐α, RANKL, and OPG were highly increased during the progress of PD in the early periods.Research HighlightsLigature‐induced bone resorption in rats occurred in the initial periods after disease inductionThe bone resorption was characterized by two distinct phases: Acute (0–14 days), with pronounced inflammation and alveolar bone lossChronic phase (14–21 days): No further disease progressionSeveral pro‐inflammatory cytokines were increased during the progress of periodontitisPeer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/147007/1/jemt23101_am.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/147007/2/jemt23101.pd

Endocannabinoid system and periodontitis: mechanisms and therapeutic implications

Abstract Introduction Periodontitis is a major public health problem. Although the principle of periodontitis therapy is mainly focused on removing dental biofilm and associated factors, its physiopathology enrolls different molecular and inflammatory events related to the host immune system, as the participation of the endocannabinoid system. Objective This review aimed to explore and elucidate the mechanisms and roles of the endocannabinoid system on periodontitis physiopathology and its possibilities for future related therapies. Material and method An electronic search was carried out on the PubMed platform for studies involving the action of the endocannabinoid system on periodontitis. Result Nineteen clinical and preclinical studies were included in this narrative review. Conclusion Cannabinoid receptors type 1 and 2 are integral components of the endocannabinoid system, manifesting in various forms in the periodontal tissues. The actions and mechanisms through which cannabinoid receptors are activated in healthy or inflamed sites remain the focus of ongoing investigations. Moreover, phytocannabinoids and synthetic cannabinoids show therapeutic potential, with pre-clinical studies indicating benefits in reducing inflammation and facilitating tissue repair

Periodontal regeneration : is it still a goal in clinical periodontology?

In the last decades, Periodontal Regeneration has been one of the most discussed topics in Periodontics, attracting the attention of researchers and clinicians. This can be justified by the evident and continuous progress observed in the field, characterized by a better understanding of the biological mechanisms involved, significant improvement of operative and technical principles, and the emergence of a wide range of biomaterials available for this purpose. Together, these aspects put the theme much in evidence in the search for functional and esthetic therapeutic solutions for periodontal tissue destruction. Despite the evident evolution, periodontal regeneration may be challenging and require the clinician to carefully evaluate each case before making a therapeutic decision. With a critical reassessment of the clinical and preclinical literature, the present study aimed to discuss the topic to answer whether Periodontal Regeneration is still a goal in clinical periodontology. The main aspects involved in the probability of success or failure of regenerative approaches were considered. A greater focus was given to intrabony and furcation defects, clinical conditions with greater therapeutic predictability. Aspects such as more appropriate materials/approaches, long-term benefits and their justification for a higher initial cost were discussed for each condition. In general, deep intrabony defects associated with residual pockets and buccal/lingual class II furcation lesions have predictable and clinically relevant results. Careful selection of the case (based on patient and defect characteristics) and excellent maintenance are essential conditions to ensure initial and long-term success

Evaluation of physicochemical properties of root-end filling materials using conventional and Micro-CT tests

Objective To evaluate solubility, dimensional stability, filling ability and volumetric change of root-end filling materials using conventional tests and new Micro-CT-based methods. Material and Methods 7 Results The results suggested correlated or complementary data between the proposed tests. At 7 days, BIO showed higher solubility and at 30 days, showed higher volumetric change in comparison with MTA (p;0.05) at 7 days. At 30 days, they presented similar solubility. BIO and MTA showed higher dimensional stability than ZOE (

Effect of two corticotomy protocols on periodontal tissue and orthodontic movement

Objective: To compare two corticotomy surgical protocols in rats to verify whether they alter conventional orthodontic movement. Methodology: Sixty Wistar rats were divided into three groups – orthodontic movement (CG), orthodontic movement and corticotomy (G1) and orthodontic movement with corticotomy and decortication (G2) – and euthanized after 7 and 14 days. Tooth movement (mm), bone volume fraction and bone volume ratio to total volume (BV/TV), and bone mineral density (BMD) were evaluated by micro-CT. The total amount of bone was measured in square millimeters and expressed as the percentage of bone area in the histomorphometry. The number of positive TRAP cells and RANK/RANKL/OPG interaction were also investigated. Results: Day 14 showed a statistically significant difference in orthodontic tooth movement in CG compared with G1 (7.52 mm; p=0.009) and G2 (7.36 mm; p=0.016). A micro-CT analysis revealed a difference between CG, G1 and G2 regarding BV/TV, with G1 and G2 presenting a lower BV/TV ratio at 14 days (0.77 and 0.73 respectively); we found no statistically significant differences regarding BMD. There was a difference in the total amount of bone in the CG group between 7 and 14 days. At 14 days, CG presented a significantly higher bone percentage than G1 and G2. Regarding TRAP, G2 had more positive cells at 7 and 14 days compared with CG and G1. Conclusion: Corticotomy accelerates orthodontic movement. Decortication does not improve corticotomy efficiency

Modulation of host cell signaling pathways as a therapeutic approach in periodontal disease

Recently, new treatment approaches have been developed to target the host component of periodontal disease. This review aims at providing updated information on host-modulating therapies, focusing on treatment strategies for inhibiting signal transduction pathways involved in inflammation. Pharmacological inhibitors of MAPK, NFκB and JAK/STAT pathways are being developed to manage rheumatoid arthritis, periodontal disease and other inflammatory diseases. Through these agents, inflammatory mediators can be inhibited at cell signaling level, interfering on transcription factors activation and inflammatory gene expression. Although these drugs offer great potential to modulate host response, their main limitations are lack of specificity and developments of side effects. After overcoming these limitations, adjunctive host modulating drugs will provide new therapeutic strategies for periodontal treatment

A autoconfiança a ensinar Expressão Musical dos recém diplomados do Mestrado em Ensino de 1º e 2º Ciclos da ESELx

Os estudos realizados em diferentes sistemas educativos sobre o ensino-aprendizagem de Música no 1º Ciclo do Ensino Básico (Primary School, em Inglaterra, e École Primaire, em França) continuam a mostrar uma grande diversidade na qualidade das ofertas curriculares. Dos vários aspetos que têm sido apontados como causas prováveis para este problema procurou-se investigar os níveis de autoconfiança dos professores generalistas para lecionar a área curricular de Expressão Musical. Tendo a ESELx um Mestrado Ensino do 1º e do 2º Ciclo do Ensino Básico (ME12c) considerou-se adequado realizar um estudo baseado numa investigação de quatro Faculdades de Educação do Reino Unido em que foram inquiridos 341 finalistas dos cursos de formação inicial de Primary teachers. Assim, aplicou-se um questionário online ao universo dos 33 alunos que se diplomaram nesse curso, no ano letivo 2013/14, tendo todos eles realizado o mesmo percurso inicial, ou seja, a nossa Licenciatura em Educação Básica (LEB). Os resultados indicam que embora 70% dos recém-diplomados manifeste elevada confiança para ensinar no 1º ciclo, em geral e sem especificar a área curricular, quando inquiridos sobre ensinar especificamente Expressão Musical, apenas 20% mantém essa mesma elevada confiança. Quanto ao total de horas de formação em Música proporcionada pela ESELx no conjunto da LEB+ME12c, 70% considera que foi Insuficiente ou Manifestamente Insuficiente. No final são feitas comparações com os resultados da investigação inglesa, apresentadas propostas de melhoria do curso e de satisfação das necessidades de formação contínua dos recém-diplomados.info:eu-repo/semantics/publishedVersio

Regulation of Ghrelin Receptor by Periodontal Bacteria In Vitro

Ghrelin plays a major role in obesity-related diseases which have been shown to be associated with periodontitis. This study sought to analyze the expression of the functional receptor for ghrelin (GHS-R1a) in periodontal cells and tissues under microbial conditions in vitro and in vivo. The GHS-R1a expression in human periodontal cells challenged with the periodontopathogen Fusobacterium nucleatum, in gingival biopsies from periodontally healthy and diseased individuals, and from rats with and without ligature-induced periodontitis was analyzed by real-time PCR, immunocytochemistry, and immunofluorescence. F. nucleatum induced an initial upregulation and subsequent downregulation of GHS-R1a in periodontal cells. In rat experimental periodontitis, the GHS-R1a expression at periodontitis sites was increased during the early stage of periodontitis, but significantly reduced afterwards, when compared with healthy sites. In human gingival biopsies, periodontally diseased sites showed a significantly lower GHS-R1a expression than the healthy sites. The expression of the functional ghrelin receptor in periodontal cells and tissues is modulated by periodontal bacteria. Due to the downregulation of the functional ghrelin receptor by long-term exposure to periodontal bacteria, the anti-inflammatory actions of ghrelin may be diminished in chronic periodontal infections, which could lead to an enhanced periodontal inflammation and tissue destruction