Supernatant from Bifidobacterium Differentially Modulates Transduction Signaling Pathways for Biological Functions of Human Dendritic Cells

International audienceBACKGROUND:Probiotic bacteria have been shown to modulate immune responses and could have therapeutic effects in allergic and inflammatory disorders. However, the signaling pathways engaged by probiotics are poorly understood. We have previously reported that a fermentation product from Bifidobacterium breve C50 (BbC50sn) could induce maturation, high IL-10 production and prolonged survival of DCs via a TLR2 pathway. We therefore studied the roles of mitogen-activated protein kinases (MAPK), glycogen synthase kinase-3 (GSK3) and phosphatidylinositol 3-kinase (PI3K) pathways on biological functions of human monocyte-derived DCs treated with BbC50sn.METHODOLOGY/PRINCIPAL FINDINGS:DCs were differentiated from human monocytes with IL-4 and GM-CSF for 5 days and cultured with BbC50sn, lipopolysaccharide (LPS) or Zymosan, with or without specific inhibitors of p38MAPK (SB203580), ERK (PD98059), PI3K (LY294002) and GSK3 (SB216763). We found that 1) the PI3K pathway was positively involved in the prolonged DC survival induced by BbC50sn, LPS and Zymosan in contrast to p38MAPK and GSK3 which negatively regulated DC survival; 2) p38MAPK and PI3K were positively involved in DC maturation, in contrast to ERK and GSK3 which negatively regulated DC maturation; 3) ERK and PI3K were positively involved in DC-IL-10 production, in contrast to GSK3 that was positively involved in DC-IL-12 production whereas p38MAPK was positively involved in both; 4) BbC50sn induced a PI3K/Akt phosphorylation similar to Zymosan and a p38MAPK phosphorylation similar to LPS.CONCLUSION/SIGNIFICANCE:We report for the first time that a fermentation product of a bifidobacteria can differentially activate MAPK, GSK3 and PI3K in order to modulate DC biological functions. These results give new insights on the fine-tuned balance between the maintenance of normal mucosal homeostasis to commensal and probiotic bacteria and the specific inflammatory immune responses to pathogen bacteria

Essential role of the initial activation signal in isotype selection upon deletion of a transcriptionally committed promoter

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Two modes of cis-activation of switch transcription by the IgH superenhancer

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The immunoglobulin heavy chain super enhancer controls class switch recombination in developing B cells

International audienceAbstractClass switch recombination (CSR) plays an important role in adaptive immune response by enabling mature B cells to replace the initial IgM by another antibody class (IgG, IgE or IgA). CSR is preceded by transcription of the IgH constant genes and is controlled by the super-enhancer 3′ regulatory region (3′RR) in an activation-specific manner. The 3’RR is composed of four enhancers (hs3a, hs1-2, hs3b and hs4). In mature B cells, 3’RR activity correlates with transcription of its enhancers. CSR can also occur in primary developing B cells though at low frequency, but in contrast to mature B cells, the transcriptional elements that regulate the process in developing B cells are ill-known. In particular, the role of the 3’RR in the control of constant genes’ transcription and CSR has not been addressed. Here, by using a mouse line devoid of the 3’RR and a culture system that highly enriches in pro-B cells, we show that the 3’RR activity is indeed required for switch transcription and CSR, though its effect varies in an isotype-specific manner and correlates with transcription of hs4 enhancer only

Developmental regulation of DNA cytosine methylation at the immunoglobulin heavy chain constant locus.

DNA cytosine methylation is involved in the regulation of gene expression during development and its deregulation is often associated with disease. Mammalian genomes are predominantly methylated at CpG dinucleotides. Unmethylated CpGs are often associated with active regulatory sequences while methylated CpGs are often linked to transcriptional silencing. Previous studies on CpG methylation led to the notion that transcription initiation is more sensitive to CpG methylation than transcriptional elongation. The immunoglobulin heavy chain (IgH) constant locus comprises multiple inducible constant genes and is expressed exclusively in B lymphocytes. The developmental B cell stage at which methylation patterns of the IgH constant genes are established, and the role of CpG methylation in their expression, are unknown. Here, we find that methylation patterns at most cis-acting elements of the IgH constant genes are established and maintained independently of B cell activation or promoter activity. Moreover, one of the promoters, but not the enhancers, is hypomethylated in sperm and early embryonic cells, and is targeted by different demethylation pathways, including AID, UNG, and ATM pathways. Combined, the data suggest that, rather than being prominently involved in the regulation of the IgH constant locus expression, DNA methylation may primarily contribute to its epigenetic pre-marking

BbC50sn induced both Akt and p38MAPK phosphorylations.

<p>Akt and p38MAPK phosphorylations induced by either BbC50sn or LPS or Zymosan in DC were evaluated at different times by flow cytometry and expressed in mean fluorescence intensity (MFI) for A) Akt; B) p38MAPK. Black histograms represent staining of phosphorylated kinases and empty or gray histograms correspond to isotype controls for Akt and p38MAPK respectively. These results are representative of one out of 2 experiments.</p

Scheme of putative signaling pathways involved in cytokine production induced by BbC50sn, LPS or Zymosan in DCs.

<p>PI3K decreased IL-12 and increased IL-10 production with BbC50sn and Zymosan through (1) phosphorylation of Akt which inhibits GSK3 (inhibitor of CREB = IL-10 nuclear factor); (2) phosphorylation of ERK which positively regulates IL-10 in contrast to IL-12 production; (3) phosphorylation of p38MAPK which positively regulates IL-10 and IL-12 productions. Putative main pathways are represented in bold.</p