Kandaki ender tümör hücrelerinin mikroakışkan kanal içi biyo-aktif oksit yüzeyde yakalanması

Isolation and characterization of circulating tumor cells (CTCs) have important clinical significance in terms of prognosis and early detection of response to treatment. Moreover, downstream characterization of CTCs may help better patient stratification and therapy guidance. However, CTCs are extremely rare (~10 CTCs/1010 peripheral blood cells) and highly sensitive, and specific technology is required for their isolation. Rapidly developing microfluidic technologies offer variety of advantages in rare cell isolation including rapid, low-cost and automated sample procesing, and higher sensitivity and specificity due to their similar physical dimenions to biological cells. Many of these technologies utilize immuno-affinity based CTC capture, where anti-EpCAM antibody against epithelial cell surface biomarker is widely utilized for CTC-specific cell capture. In such applications, a proper antibody immobilization plays a crucial role for high efficiency cell capture. In this study, development and evaluation of four different surface modification approaches to immobilize anti-EpCAM on the silicon oxide surfaces was presented and the selected modification method is implemented in microfluidic channels. Cell capture efficiency and capture specificity were determined using different breast cancer cell lines as a CTC model. Selective CTC capture was demonstrated using breast cancer cells (MCF-7) spiked in buffer containing background leukocytes with cell concentration ratio of 1:104. Cell capture efficiency and specificity of 90% and 87% have been achieved, respectively, with MCF7 cells. Besides, protocols for in-channel immunofluorecent cell staining and viable cell release have been developed. Shelf life of the functionalized surfaces was also determined by inspecting the capture efficiency of microchannels weekly.M.S. - Master of Scienc

Capture and release of viable CTCs in microfluidic channel

The number of circulating tumor cells (CTCs) in blood is associated with prognosis in several types of cancer. Isolation and characterization of CTCs have important clinical significance in terms of prognosis and early detection of response to treatment. Moreover, downstream characterization of CTCs may help better patient stratification and therapy guidance. However, CTCs are extremely rare and highly sensitive and specific technology is required to isolate viable CTCs from blood cells. In this study, a surface modification strategy is developed for (i) on-chip detection of CTCs by using immunostaining and (ii) viable cell release for downstream analysis. In the first application, selective CTC capture is demonstrated using breast cancer cells (MCF-7) spiked in buffer containing background leukocytes with cell concentration ratio of 1:100. Cells captured by anti-EpCAM coated microchannel are stained with immunofluorescence conjugated anti-pan cytokeratin antibody for epithelial cells and anti-CD45 antibody for hematologic cells, and by DAPI for nuclear staining. Cytokeratin and DAPI stained cells are scored as CTCs whereas CD45+ cells are scored as leukocytes. In the second application, captured cells are released with the combination of enzymatic treatment and high flow rate washing. The viability of released cells is confirmed by observing cell growth in culture

Development of EpCAM Sensitive Surface Functionalization Strategy for CTC Capture in Microfluidic Channels

Dolaşımdaki tümör hücrelerinin (CTC'ler) izolasyonu ve karakterizasyonu, prognoz ve tedaviye yanıtın erken tespiti açısından önemli klinik öneme sahiptir. Ayrıca, CTC'lerin aşağı akış karakterizasyonu, daha iyi hasta sınıflandırmasına ve tedavi kılavuzluğuna yardımcı olabilir. Bununla birlikte, CTC'ler son derece nadirdir (~ 10 CTC / 1010 periferik kan hücresi) ve oldukça hassastır ve izolasyonları için özel teknoloji gereklidir. Hızla gelişen mikroakışkan teknolojiler, nadir hücre izolasyonunda hızlı, düşük maliyetli ve otomatik numune işleme dahil olmak üzere çeşitli avantajlar ve biyolojik hücrelere benzer fiziksel boyutları nedeniyle daha yüksek hassasiyet ve özgüllük sunar. Bu teknolojilerin çoğu, immünoafinite tabanlı CTC yakalama kullanır, epitel hücre yüzeyi biyobelirtecine karşı anti-EpCAM antikoru, CTC'ye özgü hücre yakalama için yaygın olarak kullanılmaktadır. Bu tür uygulamalarda, uygun bir antikor immobilizasyonu, yüksek verimli hücre yakalama için çok önemli bir rol oynar. Bu çalışmada, verimli, seçici ve EpCAM'a duyarlı CTC yakalama için bir oksit yüzey modifikasyon stratejisi geliştirilmiştir. Hücre yakalama etkinliği, bir CTC modeli olarak MCF-7 göğüs kanseri hücre dizisi kullanılarak belirlenmiş ve ortalama hücre yakalama etkinliği, 8 bağımsız deneyden% 89 olarak bulunmuştur. Seçici CTC yakalama, hücre konsantrasyonu oranı 1: 104 olan arka plan lökositleri içeren tamponda spike edilmiş göğüs kanseri hücreleri (MCF-7) kullanılarak gösterildi ve sırasıyla% 90 ve% 87'lik yakalama verimliliği ve özgüllüğü elde edildi. Hücre yakalamada EpCAM ekspresyonunun önemi, farklı EpCAM ekspresyon seviyelerine sahip beş farklı meme kanseri hücre dizisi, yani MDA-MB-231, MDA-MB-468, SKBR3, MCF-7 ve CAMA-1 ile gösterilmiştir. Sonuçlar, hücrelerin bağlanma verimliliğinin, hücre yüzeyindeki EpCAM ekspresyon seviyesi ile orantılı olduğunu gösterdi

Epidemiological, Clinical, and Laboratory Features of Children With COVID-19 in Turkey

Objectives: The aim of this study is to identify the epidemiological, clinical, and laboratory features of coronavirus disease 2019 (COVID-19) in children

Education of healthcare personnel working with pediatric patients during covid-19 pandemic within the framework of infection control Covid-19 pandemisinde enfeksiyon kontrol çalışmaları çerçevesinde çocuk hastalarla çalışan sağlık personeli eğitimi

© 2020, AVES. All rights reserved.Objective: In the early stages of any epidemic caused by new emerging pathogens healthcare personnel is subject to a great risk. Pandemic caused by SARS-CoV-2, proved to be no exception. Many healthcare workers died in the early stages of pandemic due to inadequate precautions and insufficient protection. It is essential to protect and maintain the safety of healthcare personnel for the confinement of pandemic as well as continuity of qualified healthcare services which is already under strain. Educating healthcare personnel on appropiate use of personal protective equipment (PPE) is as essential as procuring them. Material and Methods: A survey is conducted on 4927 healthcare personnel working solely with pediatric patients from 32 different centers. Education given on PPE usage were questioned and analyzed depending on age, sex, occupation and region. Results: Among four thousand nine hundred twelve healthcare personnel from 32 different centers 91% (n= 4457) received education on PPE usage. Of those who received education only 36% was given both theoretical and applied education. Although there was no differences among different occupation groups, receiving education depended on regions. Conclusion: It is essential to educate healthcare personnel appropiately nationwidely for the continuity of qualified healthcare services during the pandemic

SARS-CoV-2 seropositivity among pediatric health care personnel just after the first peak of pandemic: A nationwide surveillance.

BACKGROUND: COVID-19 pandemic affected every single person on earth one way or the other. The healthcare personnel were no exception, their responsibilities as well as their risks being immense. METHODS: 4927 healthcare personnel all working in pediatric units at 32 hospitals from seven different regions of Turkey enrolled to the study to determine the seroprevalence of SARS Co-V-2 after the first peak wave. Point of care serologic lateral flow rapid test kit for IgM/IgG was used (Ecotest CE Assure Tech. Co. Ltd.). Seroprevalence and its association with demographic characteristics and possible risk factors were analyzed. RESULTS: Nearly 6.1% of healthcare personnel were found to be seropositive for SARS Co-V- 2. Seropositivity was more common among those who did not universally wear protective masks (10.6% vs 6.1%). Having a COVID-19 co-worker increased the likelihood of infection. The least and the most experienced personnel affected more. Most of the seropositive healthcare personnel (68%) did not have any suspicion that they had COVID-19 previously. CONCLUSIONS: Health surveillance for healthcare personnel involving routine point-of-care nucleic acid testing as well as monitoring PPE adherence would be important strategies to protect healthcare personnel from COVID-19 and to reduce nosocomial SARS-CoV-2 transmission

Evaluation of vaccination status of health care workers for recommended vaccines and their acceptance of SARS-CoV-2 vaccines

© 2022 The Author(s). Published with license by Taylor & Francis Group, LLC.Introduction: Health care workers (HCWs) are disproportionately exposed to infectious diseases and play a role in nosocomial transmission, making them a key demographic for vaccination. HCW vaccination rates are not optimal in many countries; hence, compulsory vaccination policies have been implemented in some countries. Although these policies are effective and necessary under certain conditions, resolving HCWs’ hesitancies and misconceptions about vaccines is crucial. HCWs have the advantage of direct contact with patients; hence, they can respond to safety concerns, explain the benefits of vaccination, and counter antivaccine campaigns that escalate during pandemics, as has been observed with COVID-19. Method: A short survey was carried out in May–June 2020 on the vaccination status of HCWs working with pediatric patients with COVID-19. The survey inquired about their vaccination status (mumps/measles/rubella [MMR], varicella, influenza, and diphtheria/tetanus [dT]) and willingness to receive hypothetical future COVID-19 vaccines. The respondents were grouped according to gender, age, occupation, and region. Results: In total, 4927 HCWs responded to the survey. Most were young, healthy adults. The overall vaccination rates were 57.8% for dT in the past 10 years, 44.5% for MMR, 33.2% for varicella, and 13.5% for influenza. Vaccination rates were the highest among physicians. The majority of HCWs (81%) stated that they would be willing to receive COVID-19 vaccines. Conclusion: Although vaccination rates for well-established vaccines were low, a majority of HCWs were willing to receive COVID-19 vaccines when available. Education and administrative trust should be enhanced to increase vaccination rates among HCWs