Efeito do exercício de obestatina e tapete rolante no perfil lipídico e níveis de malondialdeído sérmio (mda) em modelo de rato

Objective: The present study investigates the effect of intraperitoneally (IP)-administrated obestatin and short-term treadmill exercise on serum malondialdehyde (MDA) and lipid profile in rat model. Materials and Methods: Forty-five rats weighing 100 ± 5g were selected and divided into five experimental groups; Group-1/control was normally kept and did not receive obestatin-included solution or exercise. Group-2 (placebo) was treated with basal solution (50 ml distilled water). Group-3 was referred to as obestatin-group which was subjected to injection during the experiment with obestatin solution (10 μg obestatin/ rat). Group-4 was known as treadmill-group which was subjected to exercise protocol (15 days treadmill training), and group-5, known as obestatin and treadmill group (OT), was subjected to injection with obestatin and exercise protocol. The exercise-groups were trained for 3-weeks. Results: the treadmill-group was decreased total cholesterol (TC) concentration (67.67 mg/dl), in comparison with control and obestatin-group (79.00 and 78.33 mg/dl, respectively). Similarly with TC level, the triglyceride and HDL cholesterol (HDL-C) was decreased in treadmill-group (‌triglyceride: 70.66 mg/dl in compared with 87.67 mg/dl in control, and HDL-C: 46.10 mg/dl in compared with 57.33 mg/dl in control; P<0.05). Also, level of MDA was decreased in treadmill- and treadmill-obestatin groups. Conclusion: short-term treadmill exercise significantly reduced plasma lipid profile and MDA levels in rat model. Nevertheless, administration of obestatin had no significant effect on lipid profile and MDA. Furthermore, obestatin-administration in combination with treadmill-exercise did not have synergic effect on plasma lipids, MDA level and total protein in rats.Objetivo: O presente estudo investiga o efeito da obestatina administrada por via intraperitoneal (IP), exercício em esteira de curta duração no malondialdeído sérico (MDA) e no perfil lipídico no modelo de ratos. Materiais e Métodos: Quarenta e cinco ratos pesando 100 ± 5g foram selecionados e divididos em cinco grupos experimentais; O grupo 1 / controle foi normalmente mantido e não recebeu solução ou exercício incluído na obestatina. O grupo 2 (placebo) foi tratado com solução basal (50 ml de água destilada). O grupo 3 foi referido como grupo de obestatina, que foi submetido a injeção durante o experimento com solução de obestatina (10 μg de obestatina / rato). O grupo 4 era conhecido como grupo de esteira que foi submetido ao protocolo de exercícios (treinamento de 15 dias em esteira) e o grupo 5, conhecido como grupo de obestatina e esteira (OT), foi submetido à injeção de obestatina e protocolo de exercício. Os grupos de exercícios foram treinados por 3 semanas. Resultados: o grupo da esteira apresentou diminuição da concentração de colesterol total (CT) (67,67 mg / dl), em comparação com o grupo controle e o grupo da obestatina (79,00 e 78,33 mg / dl, respectivamente). Da mesma forma que com o nível de CT, o triglicerídeo e o HDL-colesterol (HDL-C) diminuíram no grupo da esteira (triglicerídeo: 70,66 mg / dl em comparação com 87,67 mg / dl no controle e HDL-C: 46,10 mg / dl em comparação com 57,33 mg / dl no controle; P <0,05). Além disso, o nível de MDA diminuiu nos grupos de esteira e esteira-obestatina. Conclusão: o exercício em esteira de curta duração reduziu significativamente o perfil lipídico plasmático e os níveis de MDA no modelo de ratos. No entanto, a administração de obestatina não teve efeito significativo no perfil lipídico e no MDA. Além disso, a administração de obestatina em combinação com o exercício em esteira não teve efeito sinérgico nos lipídios plasmáticos, nível de MDA e proteína total em ratos

Effect of different rest interval lengths of resistance exercise on lipid peroxidation and creatine kinase responses

The purpose of this study was to determine the effects of two resistance exercise (RE) protocols with different rest intervals between sets on oxidative stress and exercise-induced muscle damage. For this purpose, twenty untrained males voluntarily participated in the research and were randomly assigned to one of two resistance exercise groups: a) a shorter inter-set rest interval of 90 second (RI-90; n=10); or b) a longer rest interval of 180 second (RI-180; n=10). Resistance exercise in both groups consisted of chest press (CP), “lat” pull down (LP), leg extension (LE), leg curl (LC), and back squat (BS) exercises; these were done at a load of six repetitions maximum (6 RM). Blood samples were collected from the antecubital vein preexercise, immediately post-exercise, 6, 24, and 48 hours post-exercise, and analyzed for malondiadehyde (MDA) concentration and creatine kinase (CK) activity. The results indicated that both RI-90 and RI-180 caused significant changes in the MDA response (p=.003 and p=.036 in RI-90 and RI-180, respectively); MDA significantly increased six hours post-resistance exercise in both groups. Creatine kinase activity significantly increased at the 24-hour point post-exercise in both groups and continued for 48 hours postexercise (p=.000 for RI-90 and RI-180). There was no significant difference between corresponding MDA and CK values of two groups. We conclude that the rest interval between sets of resistance exercise does not affect oxidative stress and myocellular damage

Effect of resistance training with and without vitamin D calcium chitosan nanoparticle supplements on apoptosis markers in ovariectomized rats: An experimental study

Background: Hormone therapy is one of the most effective treatments for menopausal disorders, but it may increase the risk of breast cancer, coronary heart disease, and pulmonary embolism. Objective: The present study investigated the effect of resistance training with and without vitamin D calcium (Ca++) chitosan nanoparticles on apoptosis markers in ovariectomized rats. Materials and Methods: 42 female Wistar rats were divided into 7 groups (n = 6/each). One group was assigned as the healthy controls to show the induction of menopause. The other 6 groups comprised ovariectomized (OVX) animals including: 1) vitamin D + calcium + chitosan + resistance training, 2) saline + estrogen + resistance training, 3) saline + resistance training, 4) vitamin D + calcium + chitosan, 5) saline + estrogen, and 6) OVX + control. 48 hr after the last intervention, the hippocampus tissue was extracted to measure the BCL-2-associated X (BAX), B-cell lymphoma 2 (BCL-2), and caspase-3 gene expression as well as the percentage of dead cells. Results: OVX rats demonstrated increased BAX gene expression, ratio of BAX gene expression to BCL-2, caspase-3 gene expression, and percentage of dead cells of hippocampal tissue, but decreased BCL-2 gene expression. Resistance training and vitamin D Ca++ chitosan nanoparticle supplements seemed to reverse these changes. Conclusion: The combination of resistance training and vitamin D Ca++ chitosan nanoparticle supplements may be considered a non-pharmacological treatment for OVX-induced apoptosis. Key words: Apoptosis, BCL-2-associated X protein, Caspase-3, Estrogen replacement therapy, Hormone replacement therapy

INFLUENCE OF MUSIC TYPE LISTENING ON ANAEROBIC PERFORMANCE AND SALIVARY CORTISOL IN MALES ATHLETES

Music has been widely recommended as a technique to enhance the psychophysical state of participants in sport and exercise. However, there is scant scientific evidence to clarify its proposed benefits. Therefore, the aim of this study was to determine the effect of fast and slow rhythm of music on anaerobic performance and salivary cortisol concentration in trained men. Thirty male physical education college students (ages: 25.66±3.89 yr, height: 176.65 ± 7.66 cm, body mass: 78.45±16.20 kg) voluntary participated in this study and divided to three groups: fast music, slow music, and no music(control). All subjects performed the coninghum test following a 20% grate and 14.3km/h speed on the treadmill. For measuring of cortisol, not stimulated samples of saliva collected, 15 minutes befor and immediately 5 and 30 minute after the exercise. No significant differences were found in anaerobic performance among the three groups in pretest indicating homogeneity of the groups. However, salivary cortisol no significant in anaerobic performance 5 and 30 minute after exercise as well. Summarily, Music doed not have a positive effect on performance, this study provided some support for the hypothesis that listening fast and slow music not significantly impacted during supramaximal exercise

Učinci suplementacije omega 3 masnim kiselinama na razinu oksidativnih markera u krvi, mišićno oštećenje i upalne biljege nakon treninga s opterećenjem u mladih sportaša

The present study was conducted to assess the effect of dietary omega-3 fatty acid supplementation on the levels of oxidative stress, muscle damage, and inflammatory markers after acute resistance exercise in young athletes. In a randomized double-blind design, twenty subjects were divided into two equal groups; each subject receiving three capsules per day (3000 mg) of either omega-3 or a placebo for seven days. All subjects underwent high intensity acute resistance exercise. Venous blood samples were collected one week prior to the exercise, immediately pre-exercise, and 24 hours post exercise. Malondiadehyde (MDA), plasma total antioxidant capacity (FRAP), C-reactive protein (CRP), creatine kinase (CK), and lactate dehydrogenase (LDH) were measured in the serum. MDA, CRP and CK concentrations were significantly higher 24 hours post exercise in the placebo versus the omega-3 group (p=.005). The mean of total antioxidant capacity in both groups showed no significant differences immediately pre-exercise and 24 hours post exercise (p>.005). LDH activity was significantly higher 24 hours post exercise in both groups (p=0,005). Aktivnost LDH bila je statistički značajno viša 24 sata nakon vježbanja u obje grupe (p<0,05). Rezultati ovog istraživanja pokazuju da visokointenzivne vježbe s opterećenjem izazivaju oksidativni stres, sistemske upalne procese i povećavaju razinu pokazatelja staničnih oštećenja u sportaša. Ipak, sedam dana suplementacije omega 3 masnim kiselinama mogu smanjiti navedene učinke treninga

Effect of obestation and treadmill exercise on lipid profile and serum malondialdehyde (MDA) levels in rat model

Objective: The present study investigates the effect of intraperitoneally (IP)-administrated obestatin and short-term treadmill exercise on serum malondialdehyde (MDA) and lipid profile in rat model. Materials and Methods: Forty-five rats weighing 100 ± 5g were selected and divided into five experimental groups; Group-1/control was normally kept and did not receive obestatin-included solution or exercise. Group-2 (placebo) was treated with basal solution (50 ml distilled water). Group-3 was referred to as obestatin-group which was subjected to injection during the experiment with obestatin solution (10 μg obestatin/ rat). Group-4 was known as treadmill-group which was subjected to exercise protocol (15 days treadmill training), and group-5, known as obestatin and treadmill group (OT), was subjected to injection with obestatin and exercise protocol. The exercise-groups were trained for 3-weeks. Results: the treadmill-group was decreased total cholesterol (TC) concentration (67.67 mg/dl), in comparison with control and obestatin-group (79.00 and 78.33 mg/dl, respectively). Similarly with TC level, the triglyceride and HDL cholesterol (HDL-C) was decreased in treadmill-group (‌triglyceride: 70.66 mg/dl in compared with 87.67 mg/dl in control, and HDL-C: 46.10 mg/dl in compared with 57.33 mg/dl in control; P<0.05). Also, level of MDA was decreased in treadmill- and treadmill-obestatin groups. Conclusion: short-term treadmill exercise significantly reduced plasma lipid profile and MDA levels in rat model. Nevertheless, administration of obestatin had no significant effect on lipid profile and MDA. Furthermore, obestatin-administration in combination with treadmill-exercise did not have synergic effect on plasma lipids, MDA level and total protein in rats.Objetivo: O presente estudo investiga o efeito da obestatina administrada por via intraperitoneal (IP), exercício em esteira de curta duração no malondialdeído sérico (MDA) e no perfil lipídico no modelo de ratos. Materiais e Métodos: Quarenta e cinco ratos pesando 100 ± 5g foram selecionados e divididos em cinco grupos experimentais; O grupo 1 / controle foi normalmente mantido e não recebeu solução ou exercício incluído na obestatina. O grupo 2 (placebo) foi tratado com solução basal (50 ml de água destilada). O grupo 3 foi referido como grupo de obestatina, que foi submetido a injeção durante o experimento com solução de obestatina (10 μg de obestatina / rato). O grupo 4 era conhecido como grupo de esteira que foi submetido ao protocolo de exercícios (treinamento de 15 dias em esteira) e o grupo 5, conhecido como grupo de obestatina e esteira (OT), foi submetido à injeção de obestatina e protocolo de exercício. Os grupos de exercícios foram treinados por 3 semanas. Resultados: o grupo da esteira apresentou diminuição da concentração de colesterol total (CT) (67,67 mg / dl), em comparação com o grupo controle e o grupo da obestatina (79,00 e 78,33 mg / dl, respectivamente). Da mesma forma que com o nível de CT, o triglicerídeo e o HDL-colesterol (HDL-C) diminuíram no grupo da esteira (triglicerídeo: 70,66 mg / dl em comparação com 87,67 mg / dl no controle e HDL-C: 46,10 mg / dl em comparação com 57,33 mg / dl no controle; P <0,05). Além disso, o nível de MDA diminuiu nos grupos de esteira e esteira-obestatina. Conclusão: o exercício em esteira de curta duração reduziu significativamente o perfil lipídico plasmático e os níveis de MDA no modelo de ratos. No entanto, a administração de obestatina não teve efeito significativo no perfil lipídico e no MDA. Além disso, a administração de obestatina em combinação com o exercício em esteira não teve efeito sinérgico nos lipídios plasmáticos, nível de MDA e proteína total em ratos

Effects of concurrent exercise training on the oxidative stress biomarkers concentration in elderly men

Introduction: Excessive generation of free radicals, especially reactive oxygen species (ROS) play a general role in the acceleration of aging process. Nevertheless, there is increasing evidence indicats that elderly men who are physically active are benefited from exercise-induced adaptation in cellular antioxidant defensive systems. Therefore, the aim of this study was to study the effect of concurrent training on malondyaldeyde (MDA) and carbonyl protein (PC) concentrations in elderly men. Materials and Methods: Twenty four inactive elderly men voluntarily participated in this research and were assigned in one of the training or control groups. Participants in training group performed three days per week of concurrent training for fourteen weeks. At baseline and after 14 weeks, blood samples were obtained for MDA and PC concentration measurements. Results: After 14 weeks of concurrent training, we observed a significant decrease in MDA in the training group compared with control group (p<0.05). Nevertheless, despite of abundant decrease in PC in training group after exercises, this change was not significant statistically (P=0.068). Conclusion: Hence, according to the results of this study it seems that concurrent exercise training is a suitable training method and an effective therapeutic device in modulation of oxidative stress in aged &nbsp;me

Effect of short-term creatine monohydrate supplementation on indirect markers of cellular damage in young soccer players

But : Ces dernières années, la supplémentation de créatine a retenu la plus grande attention des médias populaires et scientifiques. Néanmoins, il n’y a pas d’information valide sur les effets secondaires de ce supplément. C’est pourquoi nous formulons l’hypothèse que le chargement de monohydrate de créatine (CrM) augmente l’activité d’enzymes de sérum comme des marqueurs indirects de dommages cellulaires. Donc, l’étude présente a été faite pour étudier l’effet à cour terme d’une supplémentation en créatine sur la lactico-déshydrogénase (LDH), la créatine phosphokinase (CK) et l’isoforme CK du myocarde (CKMB) sériques chez de jeunes footballeurs. Méthodes : Dans une étude aléatoire en double aveugle, 18 jeunes footballeurs ont participé volontairement et ont été divisés en deux groupes égaux (le groupe de Créatine et le groupe de Placebo). Le premier groupe (CrM) a consommé le supplément de créatine (0,3 g/kg par jour pendant sept jours), mais le groupe de placebo (Pl) n’a pas pris des supplément. Tous les sujets ont participés à un programme d’entraînement (huit exercices, trois séries, 15 répétitions, et 60 % one repetition maximum [1RM]). Afin de détecter l’activité enzymatique (IU/L), les échantillons de sang veineux ont été prélevés avant le chargement en créatine et 24 heures après la dernière séance d’entraînement. De plus, VO2max de chaque sujet a été obtenu avec le test navette (20 m). Les données ont été exprimées en moyens (± SD) et elles ont analysées par les t-tests dépendant et indépendant en utilisant SPSS15. Résultats L’augmentation moyenne d’activité de LDH dans les deux groupes n’était pas significative à la suite de la supplémentation à court terme de créatine, tandis que, la moyenne et la limite de changement d’activité de CK (p = 0,0004 ; tCK = 5,13) et de CKMB (p < 0,05 ; tCKMB = 2,10) ont été significativement plus élevées dans le groupe de CrM par rapport au groupe de Pl (p < 0,05). Conclusion : Les résultats présents suggèrent que l’activité des enzymes de CK et de CKMB comme des marqueurs indirects de dommages cellulaires apès la supplémentations à court terme de CrM augmente chez les jeunes footballeurs. Donc, il peut être conclu que le chargement en CrM a probablement des effets significatifs défavorables sur les marqueurs sériques indirects de dommages cellulaires. Néanmoins, d’autres études seront nécessaires pour déterminer l’effet à long et court termes de la supplémentassions de créatine sur les autres marqueurs indirects de dommages cellulaires. Mots clés : Supplémentation en créatine ; Créatine kinase ; Lactico-hydrogénase ; Footballeur

Effects of ginger (Zingiber officinale Roscoe) supplementation and resistance training on some blood oxidative stress markers in obese men

Excessive adiposity increases oxidative stress, and thus may play a critical role in the pathogenesis and development of obesity-associated comorbidities, in particular atherosclerosis, diabetes mellitus, and arterial hypertension. Improved body composition, through exercise training and diet, may therefore significantly contribute to a reduction in oxidative stress. Further, some foods high in antioxidants (e.g., ginger) provide additional defense against oxidation. This study was conducted to assess the effects of ginger (Zingiber officinale Roscoe) supplementation and progressive resistance training (PRT) on some nonenzymatic blood [total antioxidant capacity (TAC) and malondialdehyde (MDA)] oxidative stress markers in obese men. Thirty-two obese males (body mass index ≥30, aged 18–30 years) were randomized to one of the following four groups: a placebo (PL; n = 8); resistance training plus placebo (RTPL; n = 8); resistance training plus ginger supplementation (RTGI; n = 8); and ginger supplementation only (GI; n = 8). Participants in the RTGI and GI groups consumed 1 g ginger/day for 10 weeks. At the same time, PRT was undertaken by the RTPL and RTGI groups three times/week. Resting blood samples were collected at baseline and at 10 weeks, and analyzed for plasma nonenzymatic TAC and MDA concentration. After the 10-week intervention, we observed significant training × ginger supplementation × resistance training interaction for TAC (p = 0.043) and significant interactions for training × resistance training and training × ginger supplementation for MDA levels (p < 0.05). The results of this study show that 10 weeks of either ginger supplementation or PRT protects against oxidative stress and therefore both of these interventions can be beneficial for obese individuals; however, when combined, the effects cancel each other out