Structural transitions of monoolein bicontinuous cubic phase induced by inclusion of protein lysozyme solutions

Inclusion of protein lysozyme molecules in lipidic monoolein cubic phase induces a transition from a $\rm Pn\bar{3}m$ structure to $\rm Im\bar{3}m$ one. Small-angle X-ray scattering (SAXS) method with high intensity synchrotron radiation enabled us to follow closely the transition depending on the conditions of lysozyme solutions. We showed that concentrated lysozyme solutions induced the appearance of the $\rm Im\bar{3}m$ structure coexisting with the $\rm Pn\bar{3}m$ structure. From the relation between the lattice parameters of these two structures it was shown that they were related by the Bonnet transformation of underlying triply periodic minimal surfaces. We found that the transition also occurred at lower lysozyme concentration when NaCl induced attraction between lysozyme molecules. The origin of the transition was considered as a frustration in the cubic phase where lysozyme molecules were highly confined. A simple estimation of the frustration was given, which took into account of the translational entropy of lysozyme molecules. At the highest concentration of lysozyme and NaCl the $\rm Im\bar{3}m$ structure was found to disappear and left only the $\rm Pn\bar{3}m$ structure. This was probably either due to the crystallization or phase separation of lysozyme solutions ongoing microscopically, which absorbed lysozyme molecules from channels of the cubic phase and thus removed the frustration.Comment: 8 pages, 5 figure

Study on efficient planning for advanced logistics network model based on robust genetic algorithm

制度:新 ; 報告番号:甲3000号 ; 学位の種類:博士(工学) ; 授与年月日:2010/2/22 ; 早大学位記番号:新525

Analisis Pemenuhan Hak Atas Restitusi Terhadap Anak Sebagai Korban Tindak Pidana Kekerasan Seksual

Sexual violence against children can have complex impacts, including physical and non-physical loss and suffering. Children who experience sexual violence need to be protected and recovered psychologically and socially. A form of legal protection for children as victims can be in the form of restitution charged to the perpetrator to fulfill the rights of children as victims. This research aims to find out how the implementation of the right to restitution for children as victims of criminal acts of sexual violence is implemented and how judges consider in making decisions that include requests for restitution for children as victims of criminal acts of sexual violence in the jurisdiction of the Bantul District Court. This research uses a type of juridical-empirical legal research and uses interviews and literature study as data collection techniques. There were 35 cases of criminal acts of sexual violence against children at the Bantul District Court from 2020 to 2022, but only 7 cases included a request for restitution against the victim in the prosecution, and there was 1 case where the judge rejected the request for restitution. In 6 cases where restitution was granted, the victim's right to restitution was not fulfilled because the defendant did not have the good faith to pay restitution

Magnetic-field-inspired navigation for quadcopter robot in unknown environments

In this paper, a magnetic-field-inspired robot navigation is used to navigate an under-actuated quad-copter towards the desired position amidst previously-unknown arbitrary-shaped convex obstacles. Taking inspiration from the phenomena of magnetic field interaction with charged particles observed in nature, the algorithm outperforms previous reactive navigation algorithms for flying robots found in the literature as it is able to reactively generate motion commands relying only on a local sensory information without prior knowledge of the obstacles' shape or location and without getting trapped in local minima configurations. The application of the algorithm in a dynamic model of quadcopter system and in the realistic model of the commercial AscTec Pelican micro-aerial vehicle confirm the superior performance of the algorithm

Implementasi Peraturan Daerah Kabupaten Bungo Nomor 6 Tahun 2015 tentang Pengelolaan Sampah di Kelurahan Tanjung Gedang Kecamatan Pasar Muara Bungo

Estimated solid waste generated in Bungo District in a day is about 143 tons, and the leading sector in waste management in Bungo Regency is the Office of the Environment, identifying problems in implementing Bungo District Regulation No. 6/2015 on Waste Management in Tanjung Gedang Village, among others: Lack Temporary Shelter, low participation and awareness of the community in maintaining cleanliness, lack of socialization from the government to the community towards the Regional Regulation. The purpose of this study is to examine in depth related to the implementation and inhibiting factors in the implementation of the Regional Regulation in Tanjung Gedang Village. The method used in this research is descriptive method with a qualitative approach. The informants in this study numbered twelve people who were determined by purposive sampling and accidental sampling techniques. The results of this study note that the implementation of Waste Management in Tanjung Gedang Urban Village has not been implemented in accordance with the established regulations, it can be seen from the implementation of rights, obligations, prohibitions, waste management, and sanctions that have not been implemented in accordance with the rules has been established. The inhibiting factors in the implementation of the Regional Regulation are: there is no location and geographically Tanjung Gedang Village is near the riverbanks, making it difficult to create a Temporary Shelter, and there is no waste handling activity. Lack of adequate waste management facilities causes low participation and public awareness in maintaining environmental cleanliness. The limited budget causes a lack of socialization of the Regional Regulation to the public, and there is no firm action from the Government on the enforcement of the norms that have been regulated therein

Surface-enhanced infrared absorption spectroscopy (SEIRAS) to probe monolayers of membrane proteins

AbstractSurface-enhanced infrared absorption spectroscopy (SEIRAS) represents a variation of conventional infrared spectroscopy and exploits the signal enhancement exerted by the plasmon resonance of nano-structured metal thin films. The surface enhancement decays in about 10nm with the distance from the surface and is, thus, perfectly suited to selectively probe monolayers of biomembranes. Peculiar to membrane proteins is their vectorial functionality, the probing of which requires proper orientation within the membrane. To this end, the metal surface used in SEIRAS is chemically modified to generate an oriented membrane protein film. Monolayers of uniformly oriented membrane proteins are formed by tethering His-tagged proteins to a nickel nitrilo-triacetic acid (Ni-NTA) modified gold surface and SEIRAS commands molecular sensitivity to probe each step of surface modification. The solid surface used as plasmonic substrate for SEIRAS, can also be employed as an electrode to investigate systems where electron transfer reactions are relevant, like e.g. cytochrome c oxidase or plant-type photosystems. Furthermore, the interaction of these membrane proteins with water-soluble proteins, like cytochrome c or hydrogenase, is studied on the molecular level by SEIRAS. The impact of the membrane potential on protein functionality is verified by monitoring light–dark difference spectra of a monolayer of sensory rhodopsin (SRII) at different applied potentials. It is demonstrated that the interpretations of all of these experiments critically depend on the orientation of the solid-supported membrane protein. Finally, future directions of SEIRAS including cellular systems are discussed. This article is part of a Special Issue entitled: FTIR in membrane proteins and peptide studies

Geopolitics or Geobody Politics? Understanding the Rise of China and its Actions in the South China Sea

The rise of China, especially within U.S. academic and policy circles, has been increasingly analyzed through a geopolitical lens. Yet geopolitics alone cannot account for the complex political mobilization of historical memory and how it frames any discussion of peace and cooperation in the region. Drawing on the concept of geobody, or how space and people are connected in a biopolitical manner, this article examines how the territorial disputes in the South China Sea are remaking the identity and interests of China. It develops an alternative theoretical understanding of Chinas rise that focuses on identity (or geobody) politics, and explores the risks involved in a further escalation of tensions for peace and cooperation in the area, and in East Asia more generally