Identificaçao e caracterizaçao de TcNUP-1, uma proteína de lâmina nuclear em Trypanosoma cruzi

Orientador: Stenio Perdigao FragosoTese (doutorado) - Universidade Federal do Paraná, Setor de Ciencias Biológicas, Programa de Pós-Graduaçao em Biologia Celular e Molecular. Defesa: Curitiba, 01/06/2006Inclui bibliografia e anexosÁrea de concentraçao: Biologia celular e molecularA lâmina nuclear é uma estrutura fibrilar protéica que está abaixo da membrana nuclear interna. Nos metazoários, as laminas são os componentes estruturais principais dessa estrutura e estão envolvidas em diversos processos nucleares. Os eucariotos que não possuem laminas apresentam proteínas distintas com funções homólogas. Recentemente foi identificada uma proteína com domínio coiled-coil em Trypanosoma brucei, NUP-1, como o componente filamentoso principal da lâmina nuclear. Entretanto, seu papel exato ainda não foi definido. Com a intenção de sugerir funções para a lâmina nuclear em tripanossomatídeos, nós caracterizamos uma proteína ortóloga à NUP-1 em Trypanosoma cruzi. A TcNUP-1 é uma proteína com alta massa molecular e domínios coiled-coil e que está localizada na periferia nuclear. Utilizando ensaio de imunoprecipitação de cromatina, nós identificamos alguns de seus sítios de ligação com o DNA. Após a análise das seqüências encontradas, pudemos observar uma associação clara com fragmentos de DNA contendo genes e pseudogenes da família das trans-sialidases, membros da família da proteína gp85 e seqüências relacionadas à SIRE, que estão comumente associadas às regiões subteloméricas dos cromossomos do T. cruzi. Baseados nos nossos resultados, nós sugerimos que TcNUP-1 é uma proteína estrutural responsável por ancorar as extremidades dos cromossomos ao envelope nuclear e que, portanto, apresenta um papel essencial na organização nuclear do T. cruziThe nuclear lamina is a structure lining the inner nuclear membrane. In metazoans, lamins are the primary structural components of nuclear lamina and are involved in several processes. Eukaryotes lacking lamins present distinct proteins with homologous functions. Recently it was identified a coiled-coil protein in Trypanosoma brucei, NUP-1, as the major filamentous component of its nuclear lamina. However, its precise role has not been defined. To shed light to this question, we characterized a homologous protein in Trypanosoma cruzi, TcNUP-1 and identified some of its in vivo DNA binding sites using chromatin immunoprecipitation assay (ChIP). We found a clear association of the TcNUP-1 with DNA sequences containing (pseudo)genes from the ts (trans- sialidase)-like family, members of gp85 gene family and sequences related to SIRE (short interspersed repetitive element), which are commonly associated to the subtelomeric region of the T. cruzi chromosomes. Based on our results, we suggest that TcNUP-1 is a structural protein having an essential role in nuclear organization by anchoring the ends of the T. cruzi chromosomes to the nuclear envelope

Slimmable Encoders for Flexible Split DNNs in Bandwidth and Resource Constrained IoT Systems

The execution of large deep neural networks (DNN) at mobile edge devices requires considerable consumption of critical resources, such as energy, while imposing demands on hardware capabilities. In approaches based on edge computing the execution of the models is offloaded to a compute-capable device positioned at the edge of 5G infrastructures. The main issue of the latter class of approaches is the need to transport information-rich signals over wireless links with limited and time-varying capacity. The recent split computing paradigm attempts to resolve this impasse by distributing the execution of DNN models across the layers of the systems to reduce the amount of data to be transmitted while imposing minimal computing load on mobile devices. In this context, we propose a novel split computing approach based on slimmable ensemble encoders. The key advantage of our design is the ability to adapt computational load and transmitted data size in real-time with minimal overhead and time. This is in contrast with existing approaches, where the same adaptation requires costly context switching and model loading. Moreover, our model outperforms existing solutions in terms of compression efficacy and execution time, especially in the context of weak mobile devices. We present a comprehensive comparison with the most advanced split computing solutions, as well as an experimental evaluation on GPU-less devices

Excerpts from the 6th Latin American Congress of Sedimentology

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Potenciais efeitos da mudança do clima sobre a hidrologia do Pantanal.

O Pantanal compreende uma área de aproximadamente 150.000 km2 na Bacia do Alto Paraguai (BAP), cujo relevo varia entre 80 e 200 m. A rede de planícies inundáveis do Pantanal está conectada com as planícies do Chaco paraguaio ao sul. Os rios de leitos rochosos nas adjacências de planalto tornam-se rios aluviais ao adentrar a planície pantaneira, a qual tem sido progressivamente preenchida por sedimentos carreados por este sistema de rios. As falhas são evidentes na morfologia da bacia, condicionada pela ocorrência de terrenos précambrianos na borda ocidental da bacia e a drenagem aluvial moderna dentro da bacia. A sedimentação ocorre em um extenso trato aluvial deposicional formado pela planície do Rio Paraguai e por vários leques fluviais.bitstream/item/151519/1/ADM161.pd

Avulsions Drive Ecosystem Services and Economic Changes in the Brazilian Pantanal Wetlands

The Pantanal wetland is a mosaic of landscapes that brings together rich biodiversity with the valuable activities of fishing, tourism and ranching. Human occupation and land use in the headwaters have intensified the rate of channel avulsions in the lower reaches of the largest megafan on the Taquari River. This study evaluates the long-term changes of landscapes in the active depositional lobe of the Taquari megafan from the perspective of local communities of pantaneiros. Maps derived from multiple decades of multispectral Landsat data have proven useful for studying land cover changes through the relationship between dry (terrestrial vegetation and soil/dry pastures) and humid landscapes (open waters, aquatic macrophytes and wet soils), as well as through Sankey diagrams and spatiotemporal mapping with Boolean operations according to the rate of dryland recovery. We found that dryland recovery associated with an older and smaller avulsion (known as Zé da Costa) is analogous to that of a most recent and much larger avulsion (known as Caronal), which is still ongoing and has greater importance due to the scale of the impacts. Land value and fish capture depreciate as the partial Caronal avulsion still evolves, increasing the likelihood of environmental conflicts. While pantaneiros no longer profit from ecosystem services of provision (e.g., livestock or fishing), dryland recovery may deliver quantifiable ecosystem services of regulation. The strengthening of partnerships among stakeholders and the implementation of environmental compensation mechanisms are central for the best management of the Pantanal\u27s megafans that ensure quality of life for all pantaneiros

AMBIENTES DEPOSICIONAIS E EVOLUÇÃO ESTRATIGRÁFICA DO CRETÁCEO SUPERIOR DA BACIA DOS PARECIS

Embora haja muitas publicações sobre as bacias cretácicas brasileiras, o entendimento sobre processos tectônicos e climáticos envolvidos na evolução das sequências continentais ainda carece de estudos, sobretudo aqueles relacionados à porção centro-oeste do país. A sequência sedimentar do Cretáceo Superior da Bacia dos Parecis, se assenta discordantemente sobre rochas vulcânicas das formações Anarí e Tapirapuã (Jurássico Inferior) e arenitos do Cretáceo Inferior da Formação Rio Ávila. É constituída por uma sucessão de estratos conglomeráticos e arenosos na base (Formação Salto das Nuvens) sobreposta por estratos arenosos no topo (Formação Utiariti). A partir da análise de fácies e arquitetura estratigráfica, foi elaborado um modelo paleoambiental e uma proposta de evolução que contempla aspectos tectônicos e climáticos dessa porção brasileira. Usando o conceito de nível de base estratigráfico e/ou a razão entre espaço de acomodação e suprimento sedimentar (A/S), foi identificado o padrão de empilhamento aluvial e eólico, que corresponde aos diferentes estágios da Tectônica Juruá. Os eventos de sedimentação que deram origem a sequência tiveram início após a gênese de uma discordância regional (Discordância K-1) entre o Cretáceo Precoce e Tardio. Durante o Cretáceo Tardio a Bacia dos Parecis abrigou um ciclo de sedimentação aluvial (Sequência 1A) e outro eólico (Sequência 1B). O primeiro registra o aumento na razão A/S onde se desenvolveram sistemas de leques retrabalhados por rios entrelaçados e dunas eólicas baixas (Trato de Sistema de Alta Acomodação – HST). Progressivamente o espaço de acomodação foi diminuindo, fazendo com que os depósitos aluviais fossem colmatados por campos de dunas (Trato de Sistema de Baixa Acomodação – LST). Nesse período a razão A/S tendeu a 1, chegando a ser negativa nos estágios finais, com possível by-pass sedimentar e gênese da Discordância K-2. A variação de fácies da base para o topo registra mudança nas condições de climáticas da bacia, passando de um ambiente mais úmido para mais seco. Dados de paleocorrentes indicam que o Alto de Tapajós ao norte da bacia esteve ativo, sendo a área fonte dos sedimentos

Structural Blocks as Flood Control in Brazilian Pantanal

The Pantanal basin formation and evolution are not well known and explained. This work brings new information showing the structural control over the physical environment of the Pantanal, one of the most important regions all around the world due to its biodiversity. We used different data sets from remote sensing data, the first set is a corrected SRTM (shuttle radar topographic mission) altimetric data and the second is a map of the major lineaments of Pantanal extracted from photointerpretation of satellite images. Both sets were confronted with the official borderlines of Pantanal and to other two limits defined by phytogeography and remote sensing images. It is possible to show that there is a good match between the internal limits of Pantanal and the interpreted lineaments. The altitudes decrease from N to S and from E to W and the slope analysis shows that this declivity is not homogeneous on these directions. The height difference between adjacent blocks is about 3 or 4 meters. Moreover, different regions have different mean altitudes and slope, although the slope inside each block is homogeneous. Considering Pantanal as a Cenozoic active basin, this paper proposes that the neotectonic controls these blocks and so the water flow in the basin, which in turn controls the fauna and flora of the region

Phylogeny, biogeography and diversification patterns of side-necked turtles (Testudines: Pleurodira)

Pleurodires or side-necked turtles are today restricted to freshwater environments of South America, Africa– Madagascar and Australia, but in the past they were distributed much more broadly, being found also on Eurasia, India and North America, and marine environments. Two hypotheses were proposed to explain this distribution; in the first, vicariance would have shaped the current geographical distribution and, in the second, extinctions constrained a previously widespread distribution. Here, we aim to reconstruct pleurodiran biogeographic history and diversification patterns based on a new phylogenetic hypothesis recovered from the analysis of the largest morphological dataset yet compiled for the lineage, testing which biogeographical process prevailed during its evolutionary history. The resulting topology generally agrees with previous hypotheses of the group and shows that most diversification shifts were related to the exploration of new niches, e.g. littoral or marine radiations. In addition, as other turtles, pleurodires do not seem to have been much affected by either the Cretaceous– Palaeogene or the Eocene–Oligocene mass extinctions. The biogeographic analyses highlight the predominance of both anagenetic and cladogenetic dispersal events and support the importance of transoceanic dispersals as a more common driver of area changes than previously thought, agreeing with previous studies with other non-turtle lineages.Fil: Ferreira, Gabriel S.. Universidade de Sao Paulo; Brasil. Senckenberg Centre For Human Evolution And Palaeoenvironment; Alemania. Universität Tübingen; AlemaniaFil: Bronzati Filho, Mario. Bayerische Staatssammlung für Paläontologie und Geologie; AlemaniaFil: Langer, Max C.. Universidade de Sao Paulo; BrasilFil: Sterli, Juliana. Museo Paleontológico Egidio Feruglio; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

Cryptospores of Iapó formation: The first evidence of early land plants from Late Ordovician in Paraná Basin, Brazil.

peer reviewe

CALCULATING BEDLOAD TRANSPORT IN RIVERS: CONCEPTS, CALCULUS ROUTINES AND APPLICATION

Rivers are immensely important to human activities such as water supply, navigation, energy generation, and agriculture. They are also an important morphodynamic agent of erosion, transport and deposition. Their capacity to transport sediment depends on their hydraulic characteristics and can be predicted by mathematical models. Several mathematical models can be used to compute bed-load transport. Each one is appropriately better for certain conditions. In this paper, we present an application built in Microsoft Excel to compute the bed-load transport in rivers based on the Van Rijn mathematical model. The Van Rijn model is appropriate for rivers transporting sandy sediments in conditions of subcritical flow. Hydraulic parameters such as channel slope, stream power, and Reynolds and Froude numbers can be calculated using the application proposed here. The application was tested in the Parana River and results from the calculations are consistent with data obtained from fieldwork surveys. The error of the application was only 20%, which shows good agreement of the model with survey values.CNPqMato Grosso do Sul Research FoundationSao Paulo Research FoundationNational Council of Technological and Scientific DevelopmentCAPESSao Paulo Research Foundation (FAPESP)Univ Estadual Paulista, Inst Geociencias & Ciencias Exatas, Ave 24A,1515, BR-13506900 Rio Claro, SP, BrazilUniv Fed Mato Grosso do Sul, Dept Geog, Ave Rio Branco 1270, BR-79304902 Corumba, MS, BrazilUniv Fed Sao Paulo, Dept Ciencias Ambientais, Rua Sao Nicolau 210, BR-09913030 Diadema, SP, BrazilUniv Kentucky, Dept Earth & Environm Sci, 101 Slone Res Bldg, Lexington, KY 40506 USAUniv Fed Sao Paulo, Dept Ciencias Ambientais, Rua Sao Nicolau 210, BR-09913030 Diadema, SP, BrazilCNPq: 140334/2015-7CNPq: 308563/2013-1CNPq: 312386/2014-1FUNDECT/CNPq: 083/2016]FAPESP: 2007/55987-3FAPESP: 2014/06889-2CNPq: 443437/2014-9CNPq: 447402/2014-5CAPES: 140334/2015-7CAPES: 308563/2013-1CAPES: 312386/2014-1FAPESP: 2014/23334-4Web of Scienc