Assessment of the Time Management and Stress among Nursing Staff in Selected Wards of Tertiary Care Hospital, Chandigarh (2017- 2018): A Descriptive Study

Nursing by its nature is an occupation which is prone to high degree of stress level and busy schedule. This study was done to assess the time management and stress among nursing staff and to assess the correlation between time management and stress among nurses. This was a descriptive study conducted in the selected wards of tertiary care hospital (Psychiatry Ward, Advance Trauma Centre, Advance Eye Centre, Maternity Ward, Communicable Disease Ward, Advance Pediatrics Centre). Data was collected by using socio-demographic profile, Nurses Time management Inventory for Personal life, Nurses Time Management Inventory for Professional life, Nursing Stress Inventory. The data was analyzed using SPSS (Version 20.0). The study revealed that about 60% of the subjects were having moderate personal time management while 55% of subjects were having moderate time management in professional life. 79% of the subjects were often stressed at working place and 38% of the subjects stated that they were very stressful. This study revealed the correlation between the time management and nursing stress which is significant at the 0,01 level. This showed that the time management and stress are inversely proportional to each other i.e. the low will be the time management; the greater will be the stress

Study of red blood cell alloimmunization in multitransfused thalassemic children of Jammu region

Introduction: Thalassemia is one of the most common genetic disorder of hemoglobin synthesis in Jammu region. Although RBC transfusion is life saving for these patients, it may be associated with some complications like RBC alloimmunization. Thus, the aim of this study was to determine the frequency of alloimmunization and the most common alloantibodies involved. Material and Methods: This was a descriptive study involving a total of 70 thalassemic patients in the age range of 2-17 years receiving regular blood transfusions, registered at SMGS Blood Bank, Jammu. Relevant clinical and laboratory data was collected with reference to age at the start of transfusions, total number of transfusions received and splenectomy status. Antibodies screening, antibody identification, and cross matching was done on allpatient samples included in the study, during the period between November 2009 and October 2010. Results: In this study, a total of six alloantibodies six patients (8.5%) and one autoantibody (1.42%) was detected. All identified alloantibodies belonged to Rh system (i.e. anti-E, in 3 patients (50%), anti D, in one patient (16.66%)) and Kell system (anti-K, in two patients (33.34%)). Higher frequency of alloimmunization was found, with increase in number of transfusions and in those who received transfusions after 1 year of age. Alloimmunization was not significantly associated with gender and splenectomy status (P-value > 0.05). Conclusion: Red cell alloantibodies developed in 8.5% of thalassemic patients and 1.42% had autoantibodies. The most common alloantibodies identified were anti Rh system antibodies (anti-E and anti-D) present in 50% and 16.66% of patients respectively. Alloimmunization is not an uncommon problem faced by blood banks and finding compatible units for regularly transfused thalassemic patients may become very difficult. In order to reduce alloimmunization, a policy for performing extended red cell phenotyping of these patients is essential and at least antigen E and Kell negative blood should be provided for transfusion to these patients

Accuracy of two different apex locators for working length determination during root canal retreatment of mandibular molars using two different retreatment files: An in vitro study

Aim: The aim of this study was to evaluate the accuracy of two different apex locators for working length determination during root canal retreatment of mandibular molars in an in vitro study. Method: Sixty extracted mandibular first molars with separate mesial canals and apical foraminae and one distal canal were selected. The mesiobuccal and distal canals were investigated; the length with the file tip at the major diameter was defined as the tooth length (TL). The canals were prepared with ProTaper files to 1 mm short of this and filled with gutta-percha and AH Plus sealer. One week later, the root fillings were removed using ProTaper and M2 retreatment files. Tooth length was remeasured and recorded as the retreatment tooth length (RTL). Then electronic measurements were taken at the major (electronic apex locator (EAL) major) and minor (EAL minor) foraminae with Root ZX and Apex ID apex locator as suggested by the instrument display. These lengths were compared with RTL and measurements 0.5 and 1 mm short of this distance. Results: For both canals, no significant difference was found between RTL and EAL major, and 0.5 mm short of RTL and EAL minor with Root ZX apex locator and protaper retreatment files. There were significant differences found between Apex ID apex locator with M2 retreatment files readings. Conclusion: Root ZX apex locator was more accurate as compared to Apex ID apex locator in determining the working length in teeth after removing the root canal obturating material

In vitro cytotoxicity, antimicrobial, and metal-chelating activity of triterpene saponins from tea seed grown in Kangra valley, India

This study was undertaken to isolate and characterize saponins from seeds of Camellia sinensis. Four triterpene saponins S1, S2, S3, and S4 were isolated by chromatography on silica (60–120 mesh), followed by purification on Sep-Pak C-18 columns. The chemical structures (S1–S4) were elucidated on the basis of 1-D and 2-D NMR. All the saponins show broad-spectrum antifungal activity against Candida albicans, Issatchenkia orientalis, Aspergillus flavus, A. niger, A. ochraceous, A. parasiticus, A. sydowii, and Trichophyton rubrum. The most susceptible test fungus was T. rubrum inhibited at a minimum inhibitory concentration of 31.25 lg/ml by all the four saponins. Cytotoxicity of these saponins was evaluated by methyl thiazole tetrazolium and sulfo-rhodamine B assays. The saponins when tested against five human cancer cells lines, viz., OVCAR-5 (ovarian carcinoma cells), MCF-7 (human breast adenocarcinoma cells), PC-3 (human prostate cancer cells), Colo-205 (colorectal adenocarcinoma cells), and HL-60 (human promyelocytic leukemia cells) showed high cytotoxicity activity (99 %) by S1 and S2 on PC-3 cells at concentration of 100 lg/ml. Similarly, when these saponins were tested against human PBMCs by lymphocytes proliferation assay, none showed significant activity. S3 (IC50 = 1.72 mg/ml) showed high metal-chelating activity at a concentration of 20 mg/ml