Development and Characterization of F2 Population for Molecular Mapping of Aluminum-Toxicity Tolerant QTL in Soybean

Keracunan aluminium merupakan salah satukendala utama dalam budidaya kedelai pada lahan masam.Pembentukan populasi F2 merupakan langkah awal yangmenentukan keberhasilan program pemuliaan tanaman. Tujuanpenelitian ini untuk membentuk dan mengkarakterisasipopulasi F2 hasil persilangan tetua toleran dan tetua pekakeracunan Al. Pembentukan populasi dilakukan menggunakanbantuan marka SSR. Dengan marka SSR populasi dapatdibentuk dengan cepat, akurat, dan efisien. Skrining genotipakedelai pada tanah masam kahat hara menghasilkan duagenotipa toleran dan dua peka. Empat persilangan tunggaldibuat untuk mendapatkan benih F1. Tanaman F1 dan F2 diidentifikasimenggunakam marka SSR Satt_070. Dua populasi(B3462 X B3293 dan B3462 X B3442) dipilih berdasarkansuperiotas fenotipa pada lahan masam dan karakteristik molekulerpasangan tetua. Karakterisasi kedua populasi di lapangmenunjukkan transgresiveness luas untuk karakter reproduksiseperti jumlah polong dan berat 100 biji. Ini mengindikasikanbahwa karakter penting lain selain karakter ketahananterhadap keracunan Al potensial untuk dipetakandari populasi ini. Metoda pembentukan populasi ini akan sangatbermanfaat bagi pemulia tanaman khususnya pemuliakedelai untuk meningkatkan efisiensi program pemuliaanketahanan terhadap keracunan Al

Development and Characterization of F2 Population for Molecular Mapping of Aluminum-Toxicity Tolerant QTL in Soybean

Keracunan aluminium merupakan salah satukendala utama dalam budidaya kedelai pada lahan masam.Pembentukan populasi F2 merupakan langkah awal yangmenentukan keberhasilan program pemuliaan tanaman. Tujuanpenelitian ini untuk membentuk dan mengkarakterisasipopulasi F2 hasil persilangan tetua toleran dan tetua pekakeracunan Al. Pembentukan populasi dilakukan menggunakanbantuan marka SSR. Dengan marka SSR populasi dapatdibentuk dengan cepat, akurat, dan efisien. Skrining genotipakedelai pada tanah masam kahat hara menghasilkan duagenotipa toleran dan dua peka. Empat persilangan tunggaldibuat untuk mendapatkan benih F1. Tanaman F1 dan F2 diidentifikasimenggunakam marka SSR Satt_070. Dua populasi(B3462 X B3293 dan B3462 X B3442) dipilih berdasarkansuperiotas fenotipa pada lahan masam dan karakteristik molekulerpasangan tetua. Karakterisasi kedua populasi di lapangmenunjukkan transgresiveness luas untuk karakter reproduksiseperti jumlah polong dan berat 100 biji. Ini mengindikasikanbahwa karakter penting lain selain karakter ketahananterhadap keracunan Al potensial untuk dipetakandari populasi ini. Metoda pembentukan populasi ini akan sangatbermanfaat bagi pemulia tanaman khususnya pemuliakedelai untuk meningkatkan efisiensi program pemuliaanketahanan terhadap keracunan Al

COSMOS 2044. Experiment K-7-19. Pineal physiology in microgravity: Relation to rat gonadal function

It is now known that the pineal organ can interact with many endocrine and nonendocrine tissues in a regulatory fashion. Given its key role in the regulation of melatonin synthesis, its high concentration, and that its levels may persist longer than the more rapidly changing melatonin, it was felt that serotonin might give a more accurate assessment of the effects of microgravity on pineal function following recovery of animals from flight. Five-hydroxyindole acetic acid (5-HIAA), a major metabolite of serotonin metabolism, was also measured. One of the most interesting concomitants to spaceflight and exposure to microgravity has been the disturbing alteration in calcium metabolism and resulting skeletal effects. Given the link between exposure to microgravity and perturbation of calcium metabolism and the fact that the pineal is apparently one of the only soft tissues to calcify, pineal calcium content was examined following spaceflight

Gene Duplication to Reveal Adaptation Clue of Plant to Environmental Stress: A Case Study of NBS-LRR Genes in Soybean

Gene duplication to reveal adaptation clue of plant to environmental stress: A case study of NBS-LRR genes in soybean. Puji Lestari, Suk-Ha Lee, I Made Tasma, and Asadi. Adaptive strategies of plant to stress are fine-tuned by adjusting several activities including molecular mechanism which involve duplicated genes responsive to environmental changes. Genes responsive to the environmental stresses which are retained after small scale duplication are part of plant genome duplication. However, less information of duplicated genes could be adaptive to environmental changes in plant. This review presents an overview of duplication events in plant genomes which impact to gene duplication in relation to environmental changes, gene duplication as an adaptation mechanism, a case of duplicated nucleotide binding site-leucine-rich repeat (NBS-LRR) genes in soybean, and the gene duplication implementation for plant breeding in Indonesia. Notably, genome duplication events generate gene duplication and contribute to adaptive evolution against environmental changes. Generalization of plants to adapt the stressful conditions also probably improves our understanding of gene duplication as a mechanism of adaptation. Several recently duplicated NBS-LRR genes in soybean retain disease resistance QTL and the differential expression convince their contribution to biotic stress resistance in soybean. Proposed models of NBS-LRR genes duplication process may help to understand these genes response to the environmental changes. The duplication of genes resistant to pest/disease particularly NBS-LRR provides important information to select breeding parents and develop molecular markers related to desease resistance to genetically improve soybean in Indonesia. Overall, it may therefore be possible to enhance breeding which targets on genes tolerance/resistance to abiotic/biotic stress, and provide a molecular basis for crop-stress protection strategy and more improved soybean varieties specified for harsh environment

A Sphere-Packing Error Exponent for Mismatched Decoding

We derive a sphere-packing error exponent for coded transmission over discrete memoryless channels with a fixed decoding metric. By studying the error probability of the code over an auxiliary channel, we find a lower bound to the probability of error of mismatched decoding. The bound is shown to decay exponentially for coding rates smaller than a new upper bound to the mismatch capacity. For rates higher than the new upper bound, the error probability is shown to be bounded away from zero. The new upper bound is shown to improve over previous upper bounds to the mismatch capacity

A Single-Letter Upper Bound to the Mismatch Capacity

We derive a single-letter upper bound to the mismatched-decoding capacity for discrete memoryless channels. The bound is expressed as the mutual information of a transformation of the channel, such that a maximum-likelihood decoding error on the translated channel implies a mismatched-decoding error in the original channel. In particular, a strong converse is shown to hold for this upper-bound: if the rate exceeds the upper-bound, the probability of error tends to 1 exponentially when the block-length tends to infinity. We also show that the underlying optimization problem is a convex-concave problem and that an efficient iterative algorithm converges to the optimal solution. In addition, we show that, unlike achievable rates in the literature, the multiletter version of the bound does not improve. A number of examples are discussed throughout the paper.European Research Council under Grant 725411, and by the Spanish Ministry of Economy and Competitiveness under Grant TEC2016-78434-C3-1-R

Determination of optimum insulation and cement plaster thickness for bungalow buildings through a simulation-statistical approach using response surface methodology

Insulating interior side of external wall and finishing by cement plaster is one of the most appropriate methods of reducing annual energy consumption in available buildings. The aim of this study is to determine the optimum expanded polystyrene (EPS) and cement plaster thickness for bungalow building in Malaysia. The present study evaluates the effect of different thermal insulation and interior cement plaster thicknesses on the annual cooling energy consumption. Furthermore, the optimum thickness of EPS and plaster is estimated based on wall type and building orientation. Two different types of walls made of concrete and brick are considered. EPS and plaster were used in the range of 20 to 100 mm and 0 to 20mm, respectively. The results show that both thermal insulation and plaster thickness have a direct effect on annual cooling energy consumption, however, the influence of wall thermal insulation thickness is more significant than cement plaster thickness. Further, the optimum EPS thickness decreases with the increment in cement plaster thickness for different orientations and wall types. The optimum EPS thickness ranges from 31.5 mm to 53.1 mm based on wall type, orientation and cement plaster thickness. Utilizing optimum EPS and cement plaster thicknesses can thus reduce annual energy consumption by about 6 to 12 in different directions. © 2019, University of Malaya. All rights reserved

Quantitative Modeling of the Equilibration of Two-Phase Solid-Liquid Fe by Atomistic Simulations on Diffusive Time Scales

In this paper, molecular dynamics (MD) simulations based on the modified-embedded atom method (MEAM) and a phase-field crystal (PFC) model are utilized to quantitatively investigate the solid-liquid properties of Fe. A set of second nearest-neighbor MEAM parameters for higherature applications are developed for Fe, and the solid-liquid coexisting approach is utilized in MD simulations to accurately calculate the melting point, expansion in melting, latent heat, and solid-liquid interface free energy, and surface anisotropy. The required input properties to determine the PFC model parameters, such as liquid structure factor and fluctuations of atoms in the solid, are also calculated from MD simulations. The PFC parameters are calculated utilizing an iterative procedure from the inputs of MD simulations. The solid-liquid interface free energy and surface anisotropy are calculated using the PFC simulations. Very good agreement is observed between the results of our calculations from MEAM-MD and PFC simulations and the available modeling and experimental results in the literature. As an application of the developed model, the grain boundary free energy of Fe is calculated using the PFC model and the results are compared against experiments

A practical methodology for optimum seismic design of RC frames for minimum damage and life-cycle cost

The design criteria in current seismic design codes are mainly to control lateral displacements and provide adequate strength to sustain expected design load combinations. However, to achieve the most economic design solutions, the life-cycle total cost (TLCC), which includes both initial structural cost and expected damage cost, should be also considered for the probable earthquakes during the lifetime of the structure. In the present study, the TLCC of the buildings is used as the main objective function for optimum seismic design of reinforced concrete (RC) frames. First, it is demonstrated that the blind increase of the reinforcement ratios does not necessarily reduce the displacement demands and the damage costs. Subsequently, a practical methodology is developed for the optimum seismic design of RC frames based on the concept of uniform damage distribution (UDD). Using an adaptive iterative procedure, the distribution of inter-storey drifts and TLCC of the floors is modified along the height of the structure. To demonstrate the efficiency of the method, 5, 8 and 12 storey RC frames are optimized using the proposed algorithm. The results indicate that, while all predefined performance targets are satisfied, the maximum inter-storey drift ratio and TLCC of the frames are considerably reduced (up to 56% and 45%, respectively) only after a few steps. The proposed method should prove useful for more efficient performance-based design of RC frames in practice