Study to assess the prevalence of human leukocyte antigen-A*3101 allele among Indian epileptic patients and its influence on safety and efficacy of antiepileptic therapy

Background: The objective was to study the prevalence of human leukocyte antigen (HLA)-A*3101 allele among epileptic patients and to assess the safety and efficacy of antiepileptic therapy.Methods: 295 subjects were selected and divided into two groups, Group I had 192 epileptic patients and Group II had 103 normal healthy controls. After written informed consent, 30 ml of mouthwash sample was collected from each subject and DNA was extracted by standard salting-out technique and used for HLA-A*3101 genotyping by two-step nested allele-specific polymerase chain reaction amplification and agarose gel electrophoresis.Results: In Group I, 12 (6.25%) of the 192 patients were tested positive for HLA-A*3101 allele and all were taking carbamazepine (CBZ). Among them, 56 (30%) subjects had developed less severe adverse effects such as headache and giddiness, skin rashes and memory disturbances, and HLA-A*3101 was present in 8 of them while 136 had no adverse effects in which 4 of them were tested positive for the allele. In Group II, 3 (2.9%) of the 103 healthy controls were tested positive for the allele. No difference was found in response to antiepileptic therapy between allele positive and negative patients.Conclusion: The present study had shown that HLA-A*3101 is prevalent in 6.25% of the Indian epileptic population under study. The presence of this allele has a significant association with the development of mild cutaneous reactions like skin rashes. However, no difference was observed in allele positive patients in response to antiepileptic therapy in comparison with allele negative patients

STUDY AND ANALYSIS OF MICROSTRUCTURE AND MECHANICAL PROPERTIES OF WELD DURING UNDERWATER WET SMAW

SMAW experiments were conducted under the water and in the air with PVA-alumina coated electrode. Influences of surrounding water on the weld geometry, weld microstructure and mechanical properties were studied. Results shown that formation of fine granular pearlite and ferrite in the wet weld. High amount of acicular ferrite (AF) in HAZ of wet weld and this amount is decreased by increasing the heat input, more ferrite content in the air weld. Two types of metal transfer mode has been observed, i.e. globular transfer mode and combined surface tension and repelled globular transfer mode. Second one is the reason for poor weld bead appearances in wet weld. Mechanical properties like toughness and hardness has been studied. Results shows improved mechanical properties at reduced cooling rate by increasing the heat flux

Cold Atmospheric Pressure Argon Plasma Jet Assisted Degradation of Malachite Green (MG) Aqueous Solution

The oxidative degradation of cold atmospheric pressure plasma assisted degradation of malachite Green (MG) was investigated in this study. Cold atmospheric pressure plasma assisted MG degradation process was carried out as a function various plasma treatment time (05, 10, and 15 mins). The % of degradation and presence carbon content in the plasma treated MG was examined by UV-Visible spectroscopy (UV-Vis) and total organic carbon (TOC) analyzer. Optical emission spectrometer was used to identify formation of various reactive species during in situ plasma treatment. The higher degradation percentage of 90% was obtained after plasma treatment time of 15 min and value of TOC also found to decreased significantly with increasing plasma treatment time.  Toxicity of the plasma-treated MG aqueous solution samples was also examined by Staphylococcus aureus (S.aureus) bacteria

Pulmonary tuberculosis in HIV individuals: Preliminary report on clinical features and response to treatment

Objectives: To study the clinical, radiological and immunological profile of pulmonary tuberculosis in HIV infected patients and assess the response to short-course chemotherapy regimens. Methods: Seventy eight patients (68 males and 10 females) with HIV infection and having symptoms suggestive of tuberculosis attending the Government Hospital for Thoracic Medicine, Tambaram or the Tuberculosis Research Centre, Chennai were studied. The diagnosis of tuberculosis was based on clinical evaluation, bacteriological examination including sputum smear and culture and chest skiagram. HIV diagnosis was based on two tests (rapid/ELISA), detecting different antigens. CD4+ T cell counts were done on all patients initially and at the end of treatment. Blood tests and skiagrams were repeated at 2 months and at the end of treatment. All the patients were treated with standard (RNTCP) short course regimens. Patients were given all the doses under supervision during the initial intensive phase and through community DOTS providers in the continuation phase. Results: Sixty five patients had culture confirmed pulmonary tuberculosis, of whom 54 had smear positive disease, initially. The radiological manifestations were varied, with 11 subjects having miliary tuberculosis, 54 with non-homogeneous opacities and 10 with cavitation. The mean CD4 cell count at intake was 192 ± 172 cells/cumm. Patients showed good initial response to treatment with significant weight gain. At the end of 2 months of treatment, 91% of patients had sputum cultures negative for Mycobacterium tuberculosis. However, the CD4 % fell significantly by the sixth month. The study is being continued to assess the long-term response to SCC of patients with HIV and tuberculosis. Conclusions: Tuberculosis has a varied clinical presentation in patients with HIV infection. The spectrum of radiographic features ranges from normal to a miliary pattern. Inspite of clinical and bacteriological improvement during treatment, immunologic deterioration may continue

SilkSatDb: a microsatellite database of the silkworm, Bombyx mori

The SilkSatDb (silkmoth microsatellite database) (http://www.cdfd.org.in/silksatdb) is a relational database of microsatellites extracted from the available expressed sequence tags and whole genome shotgun sequences of the silkmoth, Bombyx mori. The database has been rendered with a simple and robust web-based search facility, developed using PHP. The SilkSatDb also stores information on primers developed and validated in the laboratory. Users can retrieve information on the microsatellite and the protocols used, along with informative figures and polymorphism status of those microsatellites. In addition, the interface is coupled with Autoprimer, a primer-designing program, using which users can design primers for the loci of interest

Human PrimPol is a highly error-prone polymerase regulated by single-stranded DNA binding proteins

PrimPol is a recently identified polymerase involved in eukaryotic DNA damage tolerance, employed in both re-priming and translesion synthesis mechanisms to bypass nuclear and mitochondrial DNA lesions. In this report, we investigate how the enzymatic activities of human PrimPol are regulated. We show that, unlike other TLS polymerases, PrimPol is not stimulated by PCNA and does not interact with it in vivo. We identify that PrimPol interacts with both of the major single-strand binding proteins, RPA and mtSSB in vivo. Using NMR spectroscopy, we characterize the domains responsible for the PrimPol-RPA interaction, revealing that PrimPol binds directly to the N-terminal domain of RPA70. In contrast to the established role of SSBs in stimulating replicative polymerases, we find that SSBs significantly limit the primase and polymerase activities of PrimPol. To identify the requirement for this regulation, we employed two forward mutation assays to characterize PrimPol's replication fidelity. We find that PrimPol is a mutagenic polymerase, with a unique error specificity that is highly biased towards insertion-deletion errors. Given the error-prone disposition of PrimPol, we propose a mechanism whereby SSBs greatly restrict the contribution of this enzyme to DNA replication at stalled forks, thus reducing the mutagenic potential of PrimPol during genome replication

DESFILE DE LOS BOYS SCOUTS EN LA ALAMEDA DE COLÓN [Material gráfico]

ADQUIRIDA POR EL COLECCIONISTA EN LAS PALMAS G.C.NIÑAS UNIFORMADAS DESFILANDO POR LA ALAMEDA DE COLÓN.Copia digital. Madrid : Ministerio de Educación, Cultura y Deporte. Subdirección General de Coordinación Bibliotecaria, 201

CRISPR/Cas9-Mediated Mutation in XSP10 and SlSAMT Genes Impart Genetic Tolerance to Fusarium Wilt Disease of Tomato (Solanum lycopersicum L.)

Fusarium wilt is a major devastating fungal disease of tomato (Solanum lycopersicum L.) caused by Fusarium oxysporum f. sp. lycopersici (Fol) which reduces the yield and production. Xylem sap protein 10 (XSP10) and Salicylic acid methyl transferase (SlSAMT) are two putative negative regulatory genes associated with Fusarium wilt of tomato. Fusarium wilt tolerance in tomato can be developed by targeting these susceptible (S) genes. Due to its efficiency, high target specificity, and versatility, CRISPR/Cas9 has emerged as one of the most promising techniques for knocking out disease susceptibility genes in a variety of model and agricultural plants to increase tolerance/resistance to various plant diseases in recent years. Though alternative methods, like RNAi, have been attempted to knock down these two S genes in order to confer resistance in tomato against Fusarium wilt, there has been no report of employing the CRISPR/Cas9 system for this specific intent. In this study, we provide a comprehensive downstream analysis of the two S genes via CRISPR/Cas9-mediated editing of single (XSP10 and SlSAMT individually) and dual-gene (XSP10 and SlSAMT simultaneously). Prior to directly advancing on to the generation of stable lines, the editing efficacy of the sgRNA-Cas9 complex was first validated using single cell (protoplast) transformation. In the transient leaf disc assay, the dual-gene editing showed strong phenotypic tolerance to Fusarium wilt disease with INDEL mutations than single-gene editing. In stable genetic transformation of tomato at the GE1 generation, dual-gene CRISPR transformants of XSP10 and SlSAMT primarily exhibited INDEL mutations than single-gene-edited lines. The dual-gene CRISPR-edited lines (CRELs) of XSP10 and SlSAMT at GE1 generation conferred a strong phenotypic tolerance to Fusarium wilt disease compared to single-gene-edited lines. Taken together, the reverse genetic studies in transient and stable lines of tomato revealed that, XSP10 and SlSAMT function together as negative regulators in conferring genetic tolerance to Fusariumwilt disease