52 research outputs found

    Oxidative Study of Benzaldehyde by Isoqauinolinium Bromo Chromate

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    The kinetics analysis of the oxidative reaction between benzaldehyde and oxidant is quinolinium Bromo chromate was reported in aqueous 40% acetic acid medium at 313 K. The rate of reaction varies first-power of [IQBC] and [H2SO4], whereas fractional-order kinetics was observed for benzaldehyde. The rate constant gradually increases with decrease in dielectric constant of the medium. The neutral salt does not alter the rate. The metal cations (Cu++) slightly accelerate the rate of oxidation when added to reaction mixture. The study rules out the participation of keto form of substrate in complex formation. Benzoic acid was identified as the end-product in stoichiometrically 1:1 based mechanism. The rate law was derived in accordance with the kinetic results

    Integrating transcriptomic and proteomic data for accurate assembly and annotation of genomes

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    © 2017 Wong et al.; Published by Cold Spring Harbor Laboratory Press. Complementing genome sequence with deep transcriptome and proteome data could enable more accurate assembly and annotation of newly sequenced genomes. Here, we provide a proof-of-concept of an integrated approach for analysis of the genome and proteome of Anopheles stephensi, which is one of the most important vectors of the malaria parasite. To achieve broad coverage of genes, we carried out transcriptome sequencing and deep proteome profiling of multiple anatomically distinct sites. Based on transcriptomic data alone, we identified and corrected 535 events of incomplete genome assembly involving 1196 scaffolds and 868 protein-coding gene models. This proteogenomic approach enabled us to add 365 genes that were missed during genome annotation and identify 917 gene correction events through discovery of 151 novel exons, 297 protein extensions, 231 exon extensions, 192 novel protein start sites, 19 novel translational frames, 28 events of joining of exons, and 76 events of joining of adjacent genes as a single gene. Incorporation of proteomic evidence allowed us to change the designation of more than 87 predicted noncoding RNAs to conventional mRNAs coded by protein-coding genes. Importantly, extension of the newly corrected genome assemblies and gene models to 15 other newly assembled Anopheline genomes led to the discovery of a large number of apparent discrepancies in assembly and annotation of these genomes. Our data provide a framework for how future genome sequencing efforts should incorporate transcriptomic and proteomic analysis in combination with simultaneous manual curation to achieve near complete assembly and accurate annotation of genomes

    Groundwater recharge: technical aspects

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    In the area under study and in Madhya Pradesh State too, the rural population greatly depend on tube wells fitted with hand pumps or power pumps to cater to their need of safe drinking water, since surface sources are either not available or too polluted. Indiscrimate exploitation of ground water particularly for irrigation purposes has resulted in nation wide excessive recession of ground water table adversely effecting the yield of drinking water sources and some times leading to total drying of tube wells, more so in summer season. The balance between allowable draft and natural recharge seem to have been disturbed beyond redemption by natural processes. Hence there is pressing need to address this problem immediately

    Wastewater disposal through plantation

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    Wastewater disposal through plantatio

    Cell Line Techniques and Gene Editing Tools for Antibody Production: A Review

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    The present day modern formulation practices for drugs are based on newer tools and techniques toward effective utilization. The methods of antibody formulations are to be revolutionized based on techniques of cell engineering and gene editing. In the present review, we have discussed innovations in cell engineering toward production of novel antibodies for therapeutic applications. Moreover, this review deciphers the use of RNAi, ribozyme engineering, CRISPR-Cas-based techniques for better strategies for antibody production. Overall, this review describes the multidisciplinary aspects of the production of therapeutic proteins that has gained more attention due to its increasing demand

    How Caribbean Medical Students view communications

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    Background Medical student attitudes towards communication skills are important for curriculum planners and teachers. Xavier University School of Medicine (XUSOM) is a private medical school admitting students mainly from the United States and Canada. Aims Attitude of students towards communication skills has not been previously studied in the institution. Hence the present study was carried out. Method The study was carried out among the first, second, third and fourth semester undergraduate medical (MD) students at XUSOM, Aruba during July 2013 using the communication skills attitude scale (CSAS). Respondents’ age, gender, nationality, occupation of parents, place of residence of family, semester of study were noted. The positive and negative attitude scale scores were calculated and compared among different subgroups of respondents (
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