Cytotoxic effect of crude venom isolated from Sea anemone Calliactis tricolor on human cancer cell lines

601-609The present study was made to analyze the antiproliferative effect of crude venom isolated from sea anemone Calliactis tricolor against human cancer cell lines such as Human Neuroblastoma cell (SHSY5Y), Human Lung Cancer cells (A549) and Human Colon Cancer cells (HT-29). The protein profile of venom was performed by Native PAGE and subunit profile was analyzed by SDS-PAGE. The in vitro cytotoxic effect of crude venom against SHSY5Y, A549, HT-29 and Vero cell lines was evaluated by MTT assay method. All the cells exposed to crude venom showed dose-dependent cytotoxic effect with IC50 of 60 µg/ml for both SHSY5Y and A549 cells and 75 µg/ml for HT-29 cells compared to with IC50 of 100 µg/ml for the Vero cell control. The significant decrease in cell viability was observed in SHSY5Y Human Neuroblastoma cells among other cancer cells. The cellular and nuclear morphological observations revealed the loss of cell morphological integrity along with the prominent damage of nucleus in the cell. This was further confirmed by DNA fragmentation assay. Based on the preliminary results, it could be clearly stated that the crude venom of C. tricolor may have a potential anti-cancerous molecules which can be further explored and used as a tool for Neuroblastoma chemotherapy. 

Photocatalytic Activity of Biosynthesized Silver Nanoparticle from Leaf Extract of Justicia Adhatoda

International audienceIn the present study, we investigate synthesis of silver nanoparticles from leaf extract of Justicia adhatoda. The synthesized silver nanoparticles showed the Surface Plasmon Resonance peak at 425 nm. The XRD analyzed intense peaks corresponding to (111), (200), (220) and (311) bragg's reflection based on the face centered cubic structure of silver nanomaterial. In addition, spherical shape topography of nanoparticle was observed by HRSEM and EDAX indicates the presence of silver. Further, biogenic silver nanoparticles are proved its efficient photocatalytic activity against methyl orange dye

In vitro trans-differentiation of human umbilical cord derived hematopoietic stem cells into hepatocyte like cells using combination of growth factors for cell based therapy

The aim of the study was to develop a new strategy for the differentiation of hematopoietic stem cell (HSC) derived from UCB into hepatocyte like cells and also to estimate the effects of combination of fibroblast growth factor 4 (FGF 4) and hepatocyte growth factor (HGF) on hematopoietic stem cell differentiation. HSCs were isolated and purified by magnetic activated cell sorting. HSCs were induced to hepatocyte like cells under a 2-step protocol with combination of growth factors. Reverse transcription polymerase chain reaction was performed to detect multiple genes related to hepatocyte like cells development and function. Hepatocyte like morphology was illustrated by inverted repeat microscope and the secretion of albumin and α- fetoprotein by these cells was confirmed by enzyme linked immunosorbent assay. Hepatocyte like cells was observed at the end of the protocol (days 14). These differentiated cells were observed to show high expression of genes related to hepatocytes (tryptophan 2, 3-dioxygenase [TO], glucose 6-phosphate [G6P], cytokeratin 18 [CK 18], albumin and α- fetoprotein [AFP]). The quantities of albumin and AFP at day 0 were low and upon differentiation the cells were able to produce albumin and AFP at high levels. Our results show a new strategy for differentiation in a short duration, using a combination of growth factors for the differentiation of umbilical cord blood derived HSC into hepatocyte like cells under certain in vitro conditions. After further studies this approach has the potency, for widespread cell replacement therapy for liver diseases