37 research outputs found
Oda a la convocación a Cortes
Get PDFSign.: []4, *7AntepCub. anter. y post. con orlada tip. con grab. calc. y doble fileteGrab. calc. en por
Multiple stable conformations account for reversible concentration-dependent oligomerization and autoinhibition of a metamorphic metallopeptidase
Get PDF© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Molecular plasticity controls enzymatic activity: the native fold of a protein in a given environment is normally unique and at a global free-energy minimum. Some proteins, however, spontaneously undergo substantial fold switching to reversibly transit between defined conformers, the >metamorphic> proteins. Here, we present a minimal metamorphic, selective, and specific caseinolytic metallopeptidase, selecase, which reversibly transits between several different states of defined three-dimensional structure, which are associated with loss of enzymatic activity due to autoinhibition. The latter is triggered by sequestering the competent conformation in incompetent but structured dimers, tetramers, and octamers. This system, which is compatible with a discrete multifunnel energy landscape, affords a switch that provides a reversible mechanism of control of catalytic activity unique in nature. Shape shifting: A minimal metamorphic, selective, and specific caseinolytic metallopeptidase, selecase, reversibly transits between several different states of defined three-dimensional structure (monomer and tetramer represented in picture). The competent conformation is sequestered in incompetent but structured dimers, tetramers, and octamers, which are associated with loss of enzymatic activity due to autoinhibition.This study was supported in part by grants from European, Spanish, and Catalan agencies (FP7-HEALTH-2010-261460 “Gums&Joints”; FP7-PEOPLE-2011-ITN-290246 “RAPID”; FP7-HEALTH-2012-306029-2 “TRIGGER”; BFU2012-32862; CSD2006-00015; Fundació “La Marató de TV3” grant 2009-100732; 2009SGR1036; and “Pot d’Idees” FGB301793) and FPI Ph.D. fellowships from the former Spanish Ministry for Science and Technology, currently of Economy and Competitiveness, to M.L.-P. and A.C.-P. P.B. acknowledges funds from ANR-CHEX (project SPIN-HD) and ATIP-AvenirPeer Reviewe
Juana Wolf : drama de aparato en ocho actos, y diez cuadros [Ms. 500/6]
No full text15 f. ; 23 cm. — Acte quart. — Còpia de representació. — Acotacions de l'autor en lletra més petita i entre parèntesi
Juana Wolf : drama de aparato en ocho actos, y diez cuadros [Ms. 500/3]
No full text16 f. ; 23 cm. — Acte tercer. — Còpia de representació. — Acotacions de l'autor en lletra més petita i entre parèntesi
Oda a la convocación a Cortes / por J.A.
No full textCCPB000847872-4Sign.: []4, *7Antep.Cub. anter. y post. con orla tip. con grab. calc. y doble fileteGrab. calc. en port
Juana Wolf : drama de aparato en ocho actos, y diez cuadros [Ms. 500/8]
No full text16 f. ; 23 cm. — Acte quart. — Còpia de representació. — Acotacions de l'autor en lletra més petita i entre parèntesi
Juana Wolf : drama de aparato en ocho actos, y diez cuadros [Ms. 500/1]
No full text23 f. ; 23 cm. — Acte primer. — Còpia de representació. — Acotacions de l'autor en lletra més petita i entre parèntesi
Juana Wolf : drama de aparato en ocho actos, y diez cuadros [Ms. 500/7]
No full text13 f. ; 23 cm. — Acte quart. — Còpia de representació. — Acotacions de l'autor en lletra més petita i entre parèntesi
