624 research outputs found
Transcriptional Activation of Biosynthetic Gene Clusters in Filamentous Fungi
Filamentous fungi are highly productive cell factories, many of which are industrial producers of enzymes, organic acids, and secondary metabolites. The increasing number of sequenced fungal genomes revealed a vast and unexplored biosynthetic potential in the form of transcriptionally silent secondary metabolite biosynthetic gene clusters (BGCs). Various strategies have been carried out to explore and mine this untapped source of bioactive molecules, and with the advent of synthetic biology, novel applications, and tools have been developed for filamentous fungi. Here we summarize approaches aiming for the expression of endogenous or exogenous natural product BGCs, including synthetic transcription factors, assembly of artificial transcription units, gene cluster refactoring, fungal shuttle vectors, and platform strains
On the hadronic contribution to sterile neutrino production
Sterile neutrinos with masses in the keV range are considered to be a viable
candidate for warm dark matter. The rate of their production through
active-sterile neutrino transitions peaks, however, at temperatures of the
order of the QCD scale, which makes it difficult to estimate their relic
abundance quantitatively, even if the mass of the sterile neutrino and its
mixing angle were known. We derive here a relation, valid to all orders in the
strong coupling constant, which expresses the production rate in terms of the
spectral function associated with active neutrinos. The latter can in turn be
expressed as a certain convolution of the spectral functions related to various
mesonic current-current correlation functions, which are being actively studied
in other physics contexts. In the naive weak coupling limit, the appropriate
Boltzmann equations can be derived from our general formulae.Comment: 28 pages. v2: small clarifications added, published versio
Lasing from single, stationary, dye-doped glycerol/water microdroplets located on a superhydrophobic surface
We report laser emission from single, stationary, Rhodamine B-doped
glycerol/water microdroplets located on a superhydrophobic surface. In the
experiments, a pulsed, frequency-doubled Nd:YAG laser operating at 532 nm was
used as the excitation source. The microdroplets ranged in diameter from a few
to 20 um. Lasing was achieved in the red-shifted portion of the dye emission
spectrum with threshold fluences as low as 750 J/cm2. Photobleaching was
observed when the microdroplets were pumped above threshold. In certain cases,
multimode lasing was also observed and attributed to the simultaneous lasing of
two modes belonging to different sets of whispering gallery modes.Comment: to appear in Optics Communication
Author Correction:CRISPR-based transcriptional activation tool for silent genes in filamentous fungi (Scientific Reports, (2021), 11, 1, (1118), 10.1038/s41598-020-80864-3)
The Supplementary Information published with this Article contained errors. In Note S2, the text formatting including green italics, red bold, yellow underline, purple text and blue underline was omitted. The original Supplementary Information file is provided below. These errors have now been corrected in the Supplementary Information file that accompanies the original Article
Color conductivity and ladder summation in hot QCD
The color conductivity is computed at leading logarithmic order using a Kubo
formula. We show how to sum an infinite series of planar ladder diagrams,
assuming some approximations based on the dominance of soft scattering
processes between hard particles in the plasma. The result agrees with the one
obtained previously from a kinetical approach.Comment: 15 pages, 4 figures. Explanations enlarged, two figures and some refs
added, typos corrected. Final version to be published in Phys.Rev.
Resummations of free energy at high temperature
We discuss resummation strategies for free energy in quantum field theories
at nonzero temperatures T. We point out that resummations should be performed
for the short- and long-distance parts separately in order to avoid spurious
interference effects and double-counting. We then discuss and perform Pade
resummations of these two parts for QCD at high T. The resummed results are
almost invariant under variation of the renormalization and factorization
scales. We perform the analysis also in the case of the massless scalar
theory.Comment: 16 pages, revtex4, 15 eps-figures; minor typographic errors
corrected; the version as it appears in Phys.Rev.
Study of Effect on Teeth of Intermittent Fluoridation of a Community Water Supply
Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/67913/2/10.1177_00220345530320011601.pd
Towards a Nonequilibrium Quantum Field Theory Approach to Electroweak Baryogenesis
We propose a general method to compute -violating observables from
extensions of the standard model in the context of electroweak baryogenesis. It
is alternative to the one recently developed by Huet and Nelson and relies on a
nonequilibrium quantum field theory approach. The method is valid for all
shapes and sizes of the bubble wall expanding in the thermal bath during a
first-order electroweak phase transition. The quantum physics of -violation
and its suppression coming from the incoherent nature of thermal processes are
also made explicit.Comment: 19 pages, 1 figure available upon e-mail reques
Recent advances in neutrinoless double beta decay search
Even after the discovery of neutrino flavour oscillations, based on data from
atmospheric, solar, reactor, and accelerator experiments, many characteristics
of the neutrino remain unknown. Only the neutrino square-mass differences and
the mixing angle values have been estimated, while the value of each mass
eigenstate still hasn't. Its nature (massive Majorana or Dirac particle) is
still escaping. Neutrinoless double beta decay (-DBD) experimental
discovery could be the ultimate answer to some delicate questions of elementary
particle and nuclear physics. The Majorana description of neutrinos allows the
-DBD process, and consequently either a mass value could be measured or
the existence of physics beyond the standard should be confirmed without any
doubt. As expected, the -DBD measurement is a very difficult field of
application for experimentalists. In this paper, after a short summary of the
latest results in neutrino physics, the experimental status, the R&D projects,
and perspectives in -DBD sector are reviewed.Comment: 36 pages, 7 figures, To be publish in Czech Journal of Physic
Dynamical renormalization group approach to transport in ultrarelativistic plasmas: the electrical conductivity in high temperature QED
The DC electrical conductivity of an ultrarelativistic QED plasma is studied
in real time by implementing the dynamical renormalization group. The
conductivity is obtained from the realtime dependence of a dissipative kernel
related to the retarded photon polarization. Pinch singularities in the
imaginary part of the polarization are manifest as growing secular terms that
in the perturbative expansion of this kernel. The leading secular terms are
studied explicitly and it is shown that they are insensitive to the anomalous
damping of hard fermions as a result of a cancellation between self-energy and
vertex corrections. The resummation of the secular terms via the dynamical
renormalization group leads directly to a renormalization group equation in
real time, which is the Boltzmann equation for the (gauge invariant) fermion
distribution function. A direct correspondence between the perturbative
expansion and the linearized Boltzmann equation is established, allowing a
direct identification of the self energy and vertex contributions to the
collision term.We obtain a Fokker-Planck equation in momentum space that
describes the dynamics of the departure from equilibrium to leading logarithmic
order in the coupling.This determines that the transport time scale is given by
t_{tr}=(24 pi)/[e^4 T \ln(1/e)}]. The solution of the Fokker-Planck equation
approaches asymptotically the steady- state solution as sim e^{-t/(4.038
t_{tr})}.The steady-state solution leads to the conductivity sigma = 15.698
T/[e^2 ln(1/e)] to leading logarithmic order. We discuss the contributions
beyond leading logarithms as well as beyond the Boltzmann equation. The
dynamical renormalization group provides a link between linear response in
quantum field theory and kinetic theory.Comment: LaTex, 48 pages, 14 .ps figures, final version to appear in Phys.
Rev.
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