Full optimization and validation of an HPLC method for the quantitative analysis of total sugars in a soft drink

Five HPLC methods were employed for the quantitative analysis of three natural sugars namely fructose, glucose and sucrose in soft drinks. HPLC-refractive index detector (RID)-AMINO proved to be the most suitable HPLC method to carry out the latter task. For the optimum separation and response of the natural sugars the best conditions employed were column oven temperature 30 oC, flow rate 0.1 mL/min, mobile phase ratio acetonitrile:water 75:25 and they were determined by studying all possible interactions among these three parameters. Full validation of HPLC-RID-AMINO was performed in terms of system suitability test, precision check, accuracy check and robustness.                     KEY WORDS: Sugar, Soft drink, Experimental design, Validation, HPLC, System suitability   Bull. Chem. Soc. Ethiop. 2020, 34(2), 419-426 DOI: https://dx.doi.org/10.4314/bcse.v34i2.1

Laser-controlled fluorescence in two-level systems

The ability to modify the character of fluorescent emission by a laser-controlled, optically nonlinear process has recently been shown theoretically feasible, and several possible applications have already been identified. In operation, a pulse of off-resonant probe laser beam, of sufficient intensity, is applied to a system exhibiting fluorescence, during the interval of excited- state decay following the initial excitation. The result is a rate of decay that can be controllably modified, the associated changes in fluorescence behavior affording new, chemically specific information. In this paper, a two-level emission model is employed in the further analysis of this all-optical process; the results should prove especially relevant to the analysis and imaging of physical systems employing fluorescent markers, these ranging from quantum dots to green fluorescence protein. Expressions are presented for the laser-controlled fluorescence anisotropy exhibited by samples in which the fluorophores are randomly oriented. It is also shown that, in systems with suitably configured electronic levels and symmetry properties, fluorescence emission can be produced from energy levels that would normally decay nonradiatively. © 2010 American Chemical Society

Developments in the Photonic Theory of Fluorescence

Conventional fluorescence commonly arises when excited molecules relax to their ground electronic state, and most of the surplus energy dissipates in the form of photon emission. The consolidation and full development of theory based on this concept has paved the way for the discovery of several mechanistic variants that can come into play with the involvement of laser input – most notably the phenomenon of multiphoton-induced fluorescence. However, other effects can become apparent when off-resonant laser input is applied during the lifetime of the initial excited state. Examples include a recently identified scheme for laser-controlled fluorescence. Other systems of interest are those in which fluorescence is emitted from a set of two or more coupled nanoemitters. This chapter develops a quantum theoretical outlook to identify and describe these processes, leading to a discussion of potential applications ranging from all-optical switching to the generation of optical vortices

Towards Smart Tourism: An individual appreciation of Porlwi-By-Light festival: An Ordered Probit Approach

© 2016 IEEE.The festival of light is considered as one application of Smart Tourism to intelligently promote this sector. This paper examines how different socio-demographic and psychological aspects are complementary to attitude towards individual appreciation of the recent festival of light (Porlwi-By-Light). In order to obtain a precise description and understanding of individual perception, specific attempts have been carried out to control for personality traits and the anger scale of individual. The big five factor model of personality traits and individual anger scale are thereby considered. We apply a cross sectional data, captured from a drop-off survey with a sample size of 725 observations. Within an ordered probit and negative binomial framework, gender, age, residential location, educational level, family size, income, marital status, numbers of attendees at the festival, the extent to which they wish to go again to Porlwi-By-Light festival, whether it was a good season to organize the festival, the festival was appreciative, to extent to which the traffic jam affected the visitors, how the 5Es (education, entertainment, escapism, esthetics and economic value) contributed in the festival as well as personality traits and motivational factors, are found to be the conclusive factors affecting an individual appreciation at the festival. Our findings help us to draw consideration to the consequences of festival's experience and how personality-specific policies can be designed to influence public's perceptions

CN43 Addressing the professional and educational issues for the cancer nursing and allied health professions workforce: A collaborative, strategic, UK-wide approach

Background: Cancer will affect 1 in 2 of us during our lifetime. Never has the time to focus upon the cancer workforce been more critical. The importance of developing the cancer workforce has been emphasised globally, within Europe and the United Kingdom (UK). However, within the UK, there is no nationally defined career pathway or agreed overarching competency framework for cancer nurses and the allied health professionals across the spectrum of the cancer workforce. Methods: A multi partnership collaboration within the UK, including Health Education England (HEE), Macmillan Cancer Support, UK Oncology Nursing Society (UKONS) and the Royal College of Nursing (RCN), has developed the Aspirant Cancer Career and Education Development programme (ACCEnD). ACCEnD provides guidance regarding the knowledge and capabilities required by the nursing and allied health professional workforce to care for people affected by cancer. Results: An overarching career pathway and education framework for nursing and the allied health professions has been developed. To support the implementation of the framework, four workstreams, ranging in focus from pre-registration to strategic leadership, provide guidance on the learning, development and educational needs and resources required to support the workforce throughout their cancer career. Finally, ACCEnD includes the development of a digital portfolio incorporating the framework to record practitioners’ development throughout their career pathway. Conclusions: The ACCEnD programme seeks to address and provide solutions to key issues that challenge the cancer workforce both now and into the future promoting both recruitment and retention. We welcome the opportunity to present the programme to the European Oncology Nursing Society audience

Triage of children with headache at the ED: a guideline implementation study

We present a multicenter validation of a modified Manchester Triage System (MTS) flowchart for pediatric patients who present with headache to the emergency department. A prospective observational study was conducted across 5 European pediatric emergency departments. The standard MTS headache flowchart and a modified MTS headache flowchart were tested in the participating centers, and results were compared with triage categories identified by either the physician at the end of the clinical examination or the reference classification matrix (RCM). Fifty-three patients were enrolled in the preimplementation phase and 112 in the postimplementation phase. When compared with physician's triage and RCM, the modified MTS flowchart demonstrated good sensitivity (79% and 70%, respectively), specificity (77% and 76%, respectively), and a high positive likelihood ratio (9.14 and 16.75, respectively) for the identification of low-risk children. Conclusions: Our modified headache flowchart is safe and reliable in pediatric emergency settings, especially for lower classes of urgency

Triage of children with headache at the ED: a guideline implementation study.

none18Abstract We present a multicenter validation of a modified Manchester Triage System (MTS) flowchart for pediatric patients who present with headache to the emergency department. A prospective observational study was conducted across 5 European pediatric emergency departments. The standard MTS headache flowchart and a modified MTS headache flowchart were tested in the participating centers, and results were compared with triage categories identified by either the physician at the end of the clinical examination or the reference classification matrix (RCM). Fifty-three patients were enrolled in the preimplementation phase and 112 in the postimplementation phase. When compared with physician's triage and RCM, the modified MTS flowchart demonstrated good sensitivity (79% and 70%, respectively), specificity (77% and 76%, respectively), and a high positive likelihood ratio (9.14 and 16.75, respectively) for the identification of low-risk children. Conclusions: Our modified headache flowchart is safe and reliable in pediatric emergency settings, especially for lower classes of urgency. (C) 2013 Elsevier Inc. All rights reserved.noneBalossini V;Zanin A;Alberti C;Freund Y;Decobert M;Tarantino A;La Rocca M;Lacroix L;Spiri D;Lejay E;Armoogum P;Wood C;Gervaix A;Zuccotti GV;Perilongo G;Bona G;Mercier JC;Titomanlio LBalossini, V; Zanin, A; Alberti, C; Freund, Y; Decobert, M; Tarantino, A; La Rocca, M; Lacroix, L; Spiri, D; Lejay, E; Armoogum, P; Wood, C; Gervaix, A; Zuccotti, Gv; Perilongo, Giorgio; Bona, G; Mercier, Jc; Titomanlio, L