High Temperature Fatigue Crack Growth Behavior of Ti-6Al-4V

Experimental evaluation of high temperature, Fatigue Crack Growth Rate (FCGR) data for Ti-6A1-4V, a titanium alloy, is presented. The FCGR data were measured at room temperature, 175, 230, 290 and 345°C using the Direct Current Potential Difference (DCPD) technique. Compact Tension (CT) specimens were used in the program and crack growth rates (da/dN) vs. Mode I stress intensity factor ranges (ΔΚ) were plotted as a function of temperature. A temperature rise from 175 to 345°C did not cause a substantial increase in crack growth rates within the Stage II region where a linear relationship describes the behavior. Fonnation of secondary cracks, observed at higher temperatures, may have slowed the crack propagation as observed in the fractography

Phytochemical screening, antimicrobial, anti-inflammatory and anti-cancerous activities of ethanol and hexane extracts of Urochloa ramosa

Urochloa ramosa is known for its environmental benefits such as stabilization and reclamation of polluted soils, in agriculture to control root-knot nematodes infecting crops, in medicine to treat cardiovascular diseases, duodenal ulcer, hyperglycemia, nephritis and snake bites. Qualitative analyses of phytochemicals in ethanol and hexane extracts were performed by standard methods. In vitro anti-microbial assay was performed against gram positive bacteria viz., Bacillus subtilis and Staphylococcus aureus, Gram negative bacteria viz., Pseudomonas aeruginosa and Escherichia coli and fungus Candida albicans by disc diffusion method. Hexane extract of Urochloa ramosa was found to be 70% effective against Candida albicans indicating potent antifungal property. In vitro anti-inflammatory activity was performed by albumin denaturation assay, proteinase inhibition activity assay and membrane stabilization assay using various concentrations of extracts with Aspirin and Diclofenac sodium as standard. Heat induced protein denaturation was considerably prevented by ethanol and hexane extract at concentrations between 200-500 µg/ml resulting in 60 and 62% inhibition respectively. Heat induced haemolysis of erythrocyte was remarkably inhibited (59 and 68 % respectively) at concentration of 500 µg/ml. 62 and 65 % Hypotonicity induced haemolysis was also inhibited between concentration of 300-500 µg/ml in both extracts respectively. Inhibition of formation of new blood vessels by Chorioallantoic membrane (CAM) assay proved anti-angiogenic effects of extracts. Purification, characterization and structural elucidation of bioactive molecules present in ethanol and hexane extracts are needed to be explored further for assessment of better biological activites than crude extract

Population Structure of a Hybrid Clonal Group of Methicillin-Resistant Staphylococcus aureus, ST239-MRSA-III

The methicillin-resistant Staphylococcus aureus (MRSA) clonal group known as ST239-MRSA-III is notable for its hybrid origin and for causing sustained hospital epidemics worldwide since the late 1970s. We studied the population structure of this MRSA clonal group using a sample of 111 isolates that were collected over 34 years from 29 countries. Genetic variation was assessed using typing methods and novel ascertainment methods, resulting in approximately 15 kb of sequence from 32 loci for all isolates. A single most parsimonious tree, free of homoplasy, partitioned 28 haplotypes into geographically-associated clades, including prominent European, Asian, and South American clades. The rate of evolution was estimated to be approximately 100× faster than standard estimates for bacteria, and dated the most recent common ancestor of these isolates to the mid-20th century. Associations were discovered between the ST239 phylogeny and the ccrB and dru loci of the methicillin resistance genetic element, SCCmec type III, but not with the accessory components of the element that are targeted by multiplex PCR subtyping tools. In summary, the evolutionary history of ST239 can be characterized by rapid clonal diversification that has left strong evidence of geographic and temporal population structure. SCCmec type III has remained linked to the ST239 chromosome during clonal diversification, but it has undergone homoplasious losses of accessory components. These results provide a population genetics framework for the precise identification of emerging ST239 variants, and invite a re-evaluation of the markers used for subtyping SCCmec

Group B Streptococcus vaccine development: present status and future considerations, with emphasis on perspectives for low and middle income countries.

Globally, group B Streptococcus (GBS) remains the leading cause of sepsis and meningitis in young infants, with its greatest burden in the first 90 days of life. Intrapartum antibiotic prophylaxis (IAP) for women at risk of transmitting GBS to their newborns has been effective in reducing, but not eliminating, the young infant GBS disease burden in many high income countries. However, identification of women at risk and administration of IAP is very difficult in many low and middle income country (LMIC) settings, and is not possible for home deliveries. Immunization of pregnant women with a GBS vaccine represents an alternate pathway to protecting newborns from GBS disease, through the transplacental antibody transfer to the fetus in utero. This approach to prevent GBS disease in young infants is currently under development, and is approaching late stage clinical evaluation. This manuscript includes a review of the natural history of the disease, global disease burden estimates, diagnosis and existing control options in different settings, the biological rationale for a vaccine including previous supportive studies, analysis of current candidates in development, possible correlates of protection and current status of immunogenicity assays. Future potential vaccine development pathways to licensure and use in LMICs, trial design and implementation options are discussed, with the objective to provide a basis for reflection, rather than recommendations

Levels of alpha-toxin correlate with distinct phenotypic response profiles of blood mononuclear cells and with agr background of community-associated Staphylococcus aureus isolates

Epidemiological studies of Staphylococcus aureus have shown a relation between certain clones and the presence of specific virulence genes, but how this translates into virulence-associated functional responses is not fully elucidated. Here we addressed this issue by analyses of community-acquired S. aureus strains characterized with respect to antibiotic resistance, ST types, agr types, and virulence gene profiles. Supernatants containing exotoxins were prepared from overnight bacterial cultures, and tested in proliferation assays using human peripheral blood mononuclear cells (PBMC). The strains displayed stable phenotypic response profiles, defined by either a proliferative or cytotoxic response. Although, virtually all strains elicited superantigen-mediated proliferative responses, the strains with a cytotoxic profile induced proliferation only in cultures with the most diluted supernatants. This indicated that the superantigen-response was masked by a cytotoxic effect which was also confirmed by flow cytometry analysis. The cytotoxic supernatants contained significantly higher levels of α-toxin than did the proliferative supernatants. Addition of α-toxin to supernatants characterized as proliferative switched the response into cytotoxic profiles. In contrast, no effect of Panton Valentine Leukocidin, δ-toxin or phenol soluble modulin α-3 was noted in the proliferative assay. Furthermore, a significant association between agr type and phenotypic profile was found, where agrII and agrIII strains had predominantly a proliferative profile whereas agrI and IV strains had a predominantly cytotoxic profile. The differential response profiles associated with specific S. aureus strains with varying toxin production could possibly have an impact on disease manifestations, and as such may reflect specific pathotypes

Effect of Crystal Orientation on Analysis of Single-Crystal, Nickel-Based Turbine Blade Superalloys

High-cycle fatigue-induced failures in turbine and turbopump blades is a pervasive problem. Single-crystal nickel turbine blades are used because of their superior creep, stress rupture, melt resistance, and thermomechanical fatigue capabilities. Single-crystal materials have highly orthotropic properties making the position of the crystal lattice relative to the part geometry a significant and complicating factor. A fatigue failure criterion based on the maximum shear stress amplitude on the 24 octahedral and 6 cube slip systems is presented for single-crystal nickel superalloys (FCC crystal). This criterion greatly reduces the scatter in uniaxial fatigue data for PWA 1493 at 1,200 F in air. Additionally, single-crystal turbine blades used in the Space Shuttle main engine high pressure fuel turbopump/alternate turbopump are modeled using a three-dimensional finite element (FE) model. This model accounts for material orthotrophy and crystal orientation. Fatigue life of the blade tip is computed using FE stress results and the failure criterion that was developed. Stress analysis results in the blade attachment region are also presented. Results demonstrate that control of crystallographic orientation has the potential to significantly increase a component's resistance to fatigue crack growth without adding additional weight or cost

High Temperature Fatigue Crack Growth Behavior of Ti-6Al-4V

Experimental evaluation of high temperature, Fatigue Crack Growth Rate (FCGR) data for Ti-6A1-4V, a titanium alloy, is presented. The FCGR data were measured at room temperature, 175, 230, 290 and 345°C using the Direct Current Potential Difference (DCPD) technique. Compact Tension (CT) specimens were used in the program and crack growth rates (da/dN) vs. Mode I stress intensity factor ranges (ΔΚ) were plotted as a function of temperature. A temperature rise from 175 to 345°C did not cause a substantial increase in crack growth rates within the Stage II region where a linear relationship describes the behavior. Fonnation of secondary cracks, observed at higher temperatures, may have slowed the crack propagation as observed in the fractography