Hardware-in-the-Loop Simulation to Evaluate the Performance and Constraints of the Red-light Violation Warning Application on Arterial Roads

Understanding the safety and mobility impacts of Connected Vehicle (CV) applications is critical for ensuring effective implementations of these applications. This dissertation provides an assessment of the safety and mobility impacts of the Red-Light Violation Warning (RLVW), a CV-based application at signalized intersections, under pre-timed signal control and semi-actuated signal control utilizing Emulator-in-the-loop (EILS), Software-in-the-loop (SILS), and Hardware-in-the-loop simulation (HILS) environments. Modern actuated traffic signal controllers contain several features with which controllers can provide varying green intervals for actuated phases, skip phases, and terminate phases depending on the traffic demand fluctuation from cycle to cycle. With actuated traffic signal operations, there is uncertainty in the end-of-green information provided to the vehicles using CV messages. The RLVW application lacks accurate input information about when exactly a phase is going to be terminated since this termination occurs when a gap of a particular length is encountered at the detector. This study compares the results obtained with the use of these three aforementioned simulation platforms and how the use of the platforms impacts the assessed performance of the modeled CV application. In addition, the study investigates using HILS and a method to provide an Assured Green Period (AGP) which is a definitive time when the green interval will end to mitigate the uncertainties associated with the green termination and to improve the performance of the CV application. The study results showed that in the case of pre-timed signal control, there are small differences in the assessed performance when using the three simulated platforms. However, in the case of the actuated control, the utilization of EILS showed significantly different results compared to the utilization of the SILS and the HILS platforms. The use of the SILS and the HILS platforms produced similar results. The differences can be attributed to the variations in the time lag between vehicle detection and the use of this information between the EILS and the other two platforms. In addition, the results showed that the reduction in red-light running due to RLVW was significantly higher with pre-timed control compared to the reduction with semi-actuated control. The reason is the uncertainty in the end-of-green intervals provided in the messages communicated to the vehicles, as stated above. In the case of semi-actuated control, the results showed that the safety benefits of the RLVW without the use of AGP were limited. On the other hand, the study results showed that by introducing the AGP, the RLVW can reduce the number of red-light running events at signalized intersections by approximately 92% with RLVW utilization of 100%. However, the results show that the application of the AGP, as applied and assessed in this dissertation, can have increased stopped delay and approach delay under congested traffic conditions. This issue will need to be further investigated to determine the optimal setting of the AGP considering both mobility and safety impacts

Synthesis and biological assessment of new benzothiazolopyridine and benzothiazolyl- triazole derivatives as antioxidant and antibacterial agents

ABSTRACT. A novel series of benzothiazolopyridine derivatives was synthesized via interaction of -2-(benzothiazol-2-yl)-3-(4-chlorophenyl)acrylonitrile (2) with a diverse of commercially available reagents (indandione, thiobarbituric acid, and malononitrile). Moreover, a novel group of benzothiazole linked substituted 1,2,3-triazole derivatives were synthesized by exploring the chemical behavior of 5-benzothiazolyl-2-(4-chlorophenyl)-triazol-4-amine through refluxing in glacial acetic acid, condensation with phthalic anhydride, and cyanoacetylation reactions. All newly synthetized compounds have been tested for their antioxidant and antibacterial activities compared with ascorbic acid and Ampicillin as reference drugs, respectively. The benzothiazolo- pyridopyrimidine compound 6 was found the most potent antioxidant agent with IC50 = 0.015 mg/mL compared to the results of ascorbic acid (IC50 = 0.022 mg/mL). The investigated compounds showed no antibacterial properties against Gram-negative bacterial species, Pseudomonas aeruginosa and Escherichia coli. Benzothiazolopyridine derivative 5 displayed the best growth inhibition against Gram-positive bacteria, Staphylococcus aureus and Bacillus cereus with inhibition zones 24 and 20 mm, respectively.   KEY WORDS: Benzothiazole, Pyridobenzothiazole, 1,2,3-Triazole, Naphtharidine, Antioxidant   Bull. Chem. Soc. Ethiop. 2022, 36(2), 451-463.                                                              DOI: https://dx.doi.org/10.4314/bcse.v36i2.17                                                     &nbsp

Small Molecule Glycomimetics Inhibit Vascular Calcification via c-Met/Notch3/HES1 Signalling

© Copyright by the Author(s). Published by Cell Physiol Biochem Press. BACKGROUND/AIMS: Vascular calcification represents a huge clinical problem contributing to adverse cardiovascular events, with no effective treatment currently available. Upregulation of hepatocyte growth factor has been linked with vascular calcification, and thus, represent a potential target in the development of a novel therapeutic strategy. Glycomimetics have been shown to interrupt HGF-receptor signalling, therefore this study investigated the effect of novel glycomimetics on osteogenic signalling and vascular calcification in vitro. METHODS: Primary human vascular smooth muscle cells (HVSMCs) were induced by β-glycerophosphate (β-GP) and treated with 4 glycomimetic compounds (C1-C4). The effect of β-GP and C1-C4 on alkaline phosphatase (ALP), osteogenic markers and c-Met/Notch3/HES1 signalling was determined using colorimetric assays, qRT-PCR and western blotting respectively. RESULTS: C1-C4 significantly attenuated β-GP-induced calcification, as shown by Alizarin Red S staining and calcium content by day 14. In addition, C1-C4 reduced ALP activity and prevented upregulation of the osteogenic markers, BMP-2, Runx2, Msx2 and OPN. Furthermore, β-GP increased c-Met phosphorylation at day 21, an effect ameliorated by C2 and C4 and the c-Met inhibitor, crizotinib. We next interrogated the effects of the Notch inhibitor DAPT and confirmed an inhibition of β-GP up-regulated Notch3 protein by C2, DAPT and crizotinib compared to controls. Hes-1 protein upregulation by β-GP, was also significantly downregulated by C2 and DAPT. GOLD docking analysis identified a potential binding interaction of C1-C4 to HGF which will be investigated further. CONCLUSION: These findings demonstrate that glycomimetics have potent anti-calcification properties acting via HGF/c-Met and Notch signalling

Common investigation process model for internet of things forensics

Internet of Things Forensics (IoTFs) is a new discipline in digital forensics science used in the detection, acquisition, preservation, rebuilding, analyzing, and the presentation of evidence from IoT environments. IoTFs discipline still suffers from several issues and challenges that have in the recent past been documented. For example, heterogeneity of IoT infrastructures has mainly been a key challenge. The heterogeneity of the IoT infrastructures makes the IoTFs very complex, and ambiguous among various forensic domain. This paper aims to propose a common investigation processes for IoTFs using the metamodeling method called Common Investigation Process Model (CIPM) for IoTFs. The proposed CIPM consists of four common investigation processes: i) preparation process, ii) collection process, iii) analysis process and iv) final report process. The proposed CIPM can assist IoTFs users to facilitate, manage, and organize the investigation tasks

Insulin-Like Growth Factor (IGF) Binding Protein-2, Independently of IGF-1, Induces GLUT-4 Translocation and Glucose Uptake in 3T3-L1 Adipocytes

Insulin-like growth factor binding protein-2 (IGFBP-2) is the predominant IGF binding protein produced during adipogenesis and is known to increase the insulin-stimulated glucose uptake (GU) in myotubes. We investigated the IGFBP-2-induced changes in basal and insulin-stimulated GU in adipocytes and the underlying mechanisms. We further determined the role of insulin and IGF-1 receptors in mediating the IGFBP-2 and the impact of IGFBP-2 on the IGF-1-induced GU. Fully differentiated 3T3-L1 adipocytes were treated with IGFBP-2 in the presence and absence of insulin and IGF-1. Insulin, IGF-1, and IGFBP-2 induced a dose-dependent increase in GU. IGFBP-2 increased the insulin-induced GU after long-term incubation. The IGFBP-2-induced impact on GU was neither affected by insulin or IGF-1 receptor blockage nor by insulin receptor knockdown. IGFBP-2 significantly increased the phosphorylation of PI3K, Akt, AMPK, TBC1D1, and PKCζ/λ and induced GLUT-4 translocation. Moreover, inhibition of PI3K and AMPK significantly reduced IGFBP-2-stimulated GU. In conclusion, IGFBP-2 stimulates GU in 3T3-L1 adipocytes through activation of PI3K/Akt, AMPK/TBC1D1, and PI3K/PKCζ/λ/GLUT-4 signaling. The stimulatory effect of IGFBP-2 on GU is independent of its binding to IGF-1 and is possibly not mediated through the insulin or IGF-1 receptor. This study highlights the potential role of IGFBP-2 in glucose metabolism

CIPM: Common identification process model for database forensics field

Database Forensics (DBF) domain is a branch of digital forensics, concerned with the identification, collection, reconstruction, analysis, and documentation of database crimes. Different researchers have introduced several identification models to handle database crimes. Majority of proposed models are not specific and are redundant, which makes these models a problem because of the multidimensional nature and high diversity of database systems. Accordingly, using the metamodeling approach, the current study is aimed at proposing a unified identification model applicable to the database forensic field. The model integrates and harmonizes all exiting identification processes into a single abstract model, called Common Identification Process Model (CIPM). The model comprises six phases: 1) notifying an incident, 2) responding to the incident, 3) identification of the incident source, 4) verification of the incident, 5) isolation of the database server and 6) provision of an investigation environment. CIMP was found capable of helping the practitioners and newcomers to the forensics domain to control database crimes

Comparative analysis of network forensic tools and network forensics processes

Network Forensics (NFs) is a branch of digital forensics which used to detect and capture potential digital crimes over computer networked environments crime. Network Forensic Tools (NFTs) and Network Forensic Processes (NFPs) have abilities to examine networks, collect all normal and abnormal traffic/data, help in network incident analysis, and assist in creating an appropriate incident detection and reaction and also create a forensic hypothesis that can be used in a court of law. Also, it assists in examining the internal incidents and exploitation of assets, attack goals, executes threat evaluation, also by evaluating network performance. According to existing literature, there exist quite a number of NFTs and NTPs that are used for identification, collection, reconstruction, and analysing the chain of incidents that happen on networks. However, they were vary and differ in their roles and functionalities. The main objective of this paper, therefore, is to assess and see the distinction that exist between Network Forensic Tools (NFTs) and Network Forensic Processes (NFPs). Precisely, this paper focuses on comparing among four famous NFTs: Xplico, OmniPeek, NetDetector, and NetIetercept. The outputs of this paper show that the Xplico tool has abilities to identify, collect, reconstruct, and analyse the chain of incidents that happen on networks than other NF tools

Mortality from gastrointestinal congenital anomalies at 264 hospitals in 74 low-income, middle-income, and high-income countries: a multicentre, international, prospective cohort study

Background: Congenital anomalies are the fifth leading cause of mortality in children younger than 5 years globally. Many gastrointestinal congenital anomalies are fatal without timely access to neonatal surgical care, but few studies have been done on these conditions in low-income and middle-income countries (LMICs). We compared outcomes of the seven most common gastrointestinal congenital anomalies in low-income, middle-income, and high-income countries globally, and identified factors associated with mortality. // Methods: We did a multicentre, international prospective cohort study of patients younger than 16 years, presenting to hospital for the first time with oesophageal atresia, congenital diaphragmatic hernia, intestinal atresia, gastroschisis, exomphalos, anorectal malformation, and Hirschsprung's disease. Recruitment was of consecutive patients for a minimum of 1 month between October, 2018, and April, 2019. We collected data on patient demographics, clinical status, interventions, and outcomes using the REDCap platform. Patients were followed up for 30 days after primary intervention, or 30 days after admission if they did not receive an intervention. The primary outcome was all-cause, in-hospital mortality for all conditions combined and each condition individually, stratified by country income status. We did a complete case analysis. // Findings: We included 3849 patients with 3975 study conditions (560 with oesophageal atresia, 448 with congenital diaphragmatic hernia, 681 with intestinal atresia, 453 with gastroschisis, 325 with exomphalos, 991 with anorectal malformation, and 517 with Hirschsprung's disease) from 264 hospitals (89 in high-income countries, 166 in middle-income countries, and nine in low-income countries) in 74 countries. Of the 3849 patients, 2231 (58·0%) were male. Median gestational age at birth was 38 weeks (IQR 36–39) and median bodyweight at presentation was 2·8 kg (2·3–3·3). Mortality among all patients was 37 (39·8%) of 93 in low-income countries, 583 (20·4%) of 2860 in middle-income countries, and 50 (5·6%) of 896 in high-income countries (p<0·0001 between all country income groups). Gastroschisis had the greatest difference in mortality between country income strata (nine [90·0%] of ten in low-income countries, 97 [31·9%] of 304 in middle-income countries, and two [1·4%] of 139 in high-income countries; p≤0·0001 between all country income groups). Factors significantly associated with higher mortality for all patients combined included country income status (low-income vs high-income countries, risk ratio 2·78 [95% CI 1·88–4·11], p<0·0001; middle-income vs high-income countries, 2·11 [1·59–2·79], p<0·0001), sepsis at presentation (1·20 [1·04–1·40], p=0·016), higher American Society of Anesthesiologists (ASA) score at primary intervention (ASA 4–5 vs ASA 1–2, 1·82 [1·40–2·35], p<0·0001; ASA 3 vs ASA 1–2, 1·58, [1·30–1·92], p<0·0001]), surgical safety checklist not used (1·39 [1·02–1·90], p=0·035), and ventilation or parenteral nutrition unavailable when needed (ventilation 1·96, [1·41–2·71], p=0·0001; parenteral nutrition 1·35, [1·05–1·74], p=0·018). Administration of parenteral nutrition (0·61, [0·47–0·79], p=0·0002) and use of a peripherally inserted central catheter (0·65 [0·50–0·86], p=0·0024) or percutaneous central line (0·69 [0·48–1·00], p=0·049) were associated with lower mortality. // Interpretation: Unacceptable differences in mortality exist for gastrointestinal congenital anomalies between low-income, middle-income, and high-income countries. Improving access to quality neonatal surgical care in LMICs will be vital to achieve Sustainable Development Goal 3.2 of ending preventable deaths in neonates and children younger than 5 years by 2030

Mortality from gastrointestinal congenital anomalies at 264 hospitals in 74 low-income, middle-income, and high-income countries: a multicentre, international, prospective cohort study

Summary Background Congenital anomalies are the fifth leading cause of mortality in children younger than 5 years globally. Many gastrointestinal congenital anomalies are fatal without timely access to neonatal surgical care, but few studies have been done on these conditions in low-income and middle-income countries (LMICs). We compared outcomes of the seven most common gastrointestinal congenital anomalies in low-income, middle-income, and high-income countries globally, and identified factors associated with mortality. Methods We did a multicentre, international prospective cohort study of patients younger than 16 years, presenting to hospital for the first time with oesophageal atresia, congenital diaphragmatic hernia, intestinal atresia, gastroschisis, exomphalos, anorectal malformation, and Hirschsprung’s disease. Recruitment was of consecutive patients for a minimum of 1 month between October, 2018, and April, 2019. We collected data on patient demographics, clinical status, interventions, and outcomes using the REDCap platform. Patients were followed up for 30 days after primary intervention, or 30 days after admission if they did not receive an intervention. The primary outcome was all-cause, in-hospital mortality for all conditions combined and each condition individually, stratified by country income status. We did a complete case analysis. Findings We included 3849 patients with 3975 study conditions (560 with oesophageal atresia, 448 with congenital diaphragmatic hernia, 681 with intestinal atresia, 453 with gastroschisis, 325 with exomphalos, 991 with anorectal malformation, and 517 with Hirschsprung’s disease) from 264 hospitals (89 in high-income countries, 166 in middleincome countries, and nine in low-income countries) in 74 countries. Of the 3849 patients, 2231 (58·0%) were male. Median gestational age at birth was 38 weeks (IQR 36–39) and median bodyweight at presentation was 2·8 kg (2·3–3·3). Mortality among all patients was 37 (39·8%) of 93 in low-income countries, 583 (20·4%) of 2860 in middle-income countries, and 50 (5·6%) of 896 in high-income countries (p<0·0001 between all country income groups). Gastroschisis had the greatest difference in mortality between country income strata (nine [90·0%] of ten in lowincome countries, 97 [31·9%] of 304 in middle-income countries, and two [1·4%] of 139 in high-income countries; p≤0·0001 between all country income groups). Factors significantly associated with higher mortality for all patients combined included country income status (low-income vs high-income countries, risk ratio 2·78 [95% CI 1·88–4·11], p<0·0001; middle-income vs high-income countries, 2·11 [1·59–2·79], p<0·0001), sepsis at presentation (1·20 [1·04–1·40], p=0·016), higher American Society of Anesthesiologists (ASA) score at primary intervention (ASA 4–5 vs ASA 1–2, 1·82 [1·40–2·35], p<0·0001; ASA 3 vs ASA 1–2, 1·58, [1·30–1·92], p<0·0001]), surgical safety checklist not used (1·39 [1·02–1·90], p=0·035), and ventilation or parenteral nutrition unavailable when needed (ventilation 1·96, [1·41–2·71], p=0·0001; parenteral nutrition 1·35, [1·05–1·74], p=0·018). Administration of parenteral nutrition (0·61, [0·47–0·79], p=0·0002) and use of a peripherally inserted central catheter (0·65 [0·50–0·86], p=0·0024) or percutaneous central line (0·69 [0·48–1·00], p=0·049) were associated with lower mortality. Interpretation Unacceptable differences in mortality exist for gastrointestinal congenital anomalies between lowincome, middle-income, and high-income countries. Improving access to quality neonatal surgical care in LMICs will be vital to achieve Sustainable Development Goal 3.2 of ending preventable deaths in neonates and children younger than 5 years by 2030