7 research outputs found
Wet-spinnability and crosslinked fibre properties of two collagen polypeptides with varied molecular weight
The formation of naturally-derived materials with wet stable fibrous architectures is paramount in order to mimic the features of tissues at the molecular and microscopic scale. Here, we investigated the formation of wet-spun fibres based on collagen-derived polypeptides with comparable chemical composition and varied molecular weight. Gelatin and hydrolysed fish collagen (HFC) were selected as widely-available linear amino-acidic chains of high and low molecular weight, respectively, and functionalised in the wet-spun fibre state in order to preserve the material geometry in physiological conditions. Wet-spun fibre diameter and morphology were dramatically affected depending on the polypeptide molecular weight, wet-spinning solvent (i.e. 2,2,2-Trifluoroethanol and dimethyl sulfoxide) and coagulating medium (i.e. acetone and ethanol), resulting in either bulky or porous internal geometry. Dry-state tensile moduli were significantly enhanced in gelatin and HFC samples following covalent crosslinking with activated 1,3-phenylenediacetic acid (Ph) (E: 726 ± 43 ‒ 844 ± 85 MPa), compared to samples crosslinked via intramolecular carbodiimide-mediated condensation reaction (E: 588 ± 38 MPa). Resulting fibres displayed a dry diameter in the range of 238±18–355±28 μm and proved to be mechanically-stable (E: 230 kPa) following equilibration with PBS, whilst a nearly-complete degradation was observed after 5-day incubation in physiological conditions
High performance additive manufactured scaffolds for bone tissue engineering application
10.1039/c1sm05793fSoft Matter7188013-802
Chitosan-Stabilized CuO Nanostructure-Functionalized UV-Crosslinked PVA/Chitosan Electrospun Membrane as Enhanced Wound Dressing
Electrospun nanofibrous membranes are of great interest
for tissue
engineering, active material delivery, and wound dressing. These nanofibers
possess unique three-dimensional (3D) interconnected porous structures
that result in a higher surface-area-to-volume ratio and porosity.
This study was carried out to prepare nanofibrous membranes by electrospinning
a blend of PVA/chitosan polymeric solution functionalized with different
ratios of copper oxide. Chitosan-stabilized CuO nanoparticles (CH-CuO
NPs) were biosynthesized successfully utilizing chitosan as the capping
and reducing agent. XRD analysis confirmed the monoclinic structure
of CH-CuO NPs. In addition, the electrospun nanofibrous membranes
were UV-crosslinked for a definite time. The membranes containing
CH-CuO NPs were characterized by X-ray diffraction (XRD), scanning
electron microscopy (SEM), differential scanning calorimetry (DSC),
Fourier transform infrared (FTIR) spectroscopy, ultraviolet–visible
(UV–vis) spectrophotometry, and dynamic light scattering (DLS).
SEM results showed the nanosize of the fiber diameter in the range
of 147–207 nm. The FTIR spectroscopy results indicated the
successful incorporation of CH-CuO NPs into the PVA/chitosan nanofibrous
membranes. DSC analysis proved the enhanced thermal stability of the
nanofibrous membranes due to UV-crosslinking. Swelling and degradation
tests were carried out to ensure membrane stability. Greater antimicrobial
activity was observed in the nanoparticle-loaded membrane. An in vitro
release study of Cu2+ ions from the membrane was carried
out for 24 h. The cytotoxicity of CH-CuO NP-incorporated membranes
was investigated to estimate the safe dose of nanoparticles. An in
vivo test using the CH-CuO NP-loaded PVA/chitosan membrane was conducted
on a mice model, in which wound healing occurred in approximately
12 days. These results confirmed that the biocompatible, nontoxic
nanofibrous membranes are ideal for wound-dressing applications
Fabrication of polycaprolactone-silanated β-tricalcium phosphate-heparan sulfate scaffolds for spinal fusion applications
GPTMS-Modified Bredigite/PHBV Nanofibrous Bone Scaffolds with Enhanced Mechanical and Biological Properties
Biomimetic composite coating on rapid prototyped scaffolds for bone tissue engineering
10.1016/j.actbio.2010.09.010Acta Biomaterialia72809-82