11 research outputs found

    Tracking neonicotinoids following their use as cotton seed treatments

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    Neonicotinoids are a leading class of insecticides on the global market, accounting for nearly 25%. They are widely used in both agricultural and residential settings. Causing neuron failure by irreversibly binding to the insect nicotinic acetylcholine receptor, neonicotinoids offer broad spectrum efficacy against a variety of pests. However, because they are non-selective with regard to insect species, there has been some concern with neonicotinoid use over threats to pollinators such as honeybees, and potential indirect effects to migratory waterfowl as a result of invertebrate prey population depletion. In order to study occurrence and fate of neonicotinoids (thiamethoxam and imidacloprid), we analyzed cotton leaves on plants grown from neonicotinoid-treated seeds and corresponding soil samples between cotton rows. Neonicotinoid concentration data from cotton leaves appears to be consistent with the claim that seed treatments protect plants for 3–4 weeks; by 30 days post-planting, neonicotinoid concentrations fell, in general, to 200 ng/g or lower. This represents about a 10-fold decrease from plant concentrations at approximately 2 weeks post-planting. It was found that neonicotinoids used as seed treatments remained present in the soil for months post planting and could be available for runoff. To that end, 21 playa wetlands were sampled; 10 had at least one quantifiable neonicotinoid present, three of which were classified as grassland or rangeland playas, two were urban, and the remaining five were cropland playas. In several instances, neonicotinoid concentrations in playas exceeded EPA chronic benchmarks for aquatic invertebrates

    Rehabilitation of a patient with stroke

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    Stroke is a significant cause of long-term disability world-wide. The post-stroke disabilities are due to loss of locomotion, activity of daily living, cognition and communication skills. Rehabilitation is an integral part of medical management and continues longitudinally through acute care, post-acute care and community reintegration. The objectives of stroke rehabilitation are to maximize the functional independence, minimize the disabilities, reintegrate back into the home and community and improve the self-esteem of patient. A comprehensive stroke rehabilitation service should provide early assessment of impairments and disabilities, management and prevention of complications and well-organized rehabilitation program in both in-patient and out-patient settings. A multidisciplinary or interdisciplinary team approach is necessary to reduce the post-stroke disabilities. It has many members, including physicians, physical therapists, occupational therapists, speech and language pathologists, orthotist, psychotherapists, social workers, vocational rehabilitation therapists, rehabilitation nurse, patients, families and other caregivers. Physicians caring for patients with stroke during rehabilitation must be aware of potential medical complications, as well as a number of special problems that may complicate recovery, including cognitive deficits, aphasia, dysphagia, urinary incontinence, shoulder pain, spasticity, falls and depression. Involvement of patient and caregivers in the rehabilitation process is essential. This article outlines the salient features of the early comprehensive rehabilitation after stroke

    Pecan, <i>Carya illinoinensis</i>, a New Host Report of Yellowstriped Armyworm, <i>Spodoptera ornithogalli</i> (Guenee) (Lepidoptera: Noctuidae) in the Southeastern US

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    Yellowstriped armyworm (YSAW), Spodoptera ornithogalli, is a polyphagous pest that infests various crops, including cotton, cabbage, corn, blackberry, grape, etc. We documented egg clusters, larvae and adults of YSAW in pecan orchards in Georgia. Until now, there have been no reports of YSAW infesting pecan and its suitability as a host. To investigate the survival, development, and reproduction of YSAW on pecan, we used the age-stage, two sex life table. The YSAW successfully completed its lifecycle on pecan with an 82% preadult survival rate. The preadult duration and mean fecundity were 47.84 d and 1212.55 offspring per female. The population parameters, including intrinsic rate of increase (r), finite rate of increase (λ), net reproduction rate (R0), and mean generation time (T) were 0.1184 d−1, 1.1257 d−1, 430.67 offspring/female, and 51.05 d, respectively. This study confirms that pecan, Carya illinoinensis, is a potential host plant of YSAW and perhaps more widespread damage could be observed

    Peptide-based Synthetic Design, Construction and Morphology of Soft Structures

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    Peptide-based self-assembly offers a unique entry into the construction of soft structures with interesting material properties and functions. Aromatic amino acid-containing peptides are commonly employed as they exhibit high propensity to aggregate due to increased hydrophobic content, promotion of favorable secondary structures, planarity and the possibility of ?–? interactions. Incorporation of covalent scaffolds, stimuli-responsive handles and carbohydrate moieties augment beneficial characteristics to the resulting peptide conjugates. These modifications were shown to enforce self-association, elicit stimuli response and achieve improved hydrophilic properties, to name but a few

    Assessing Feeding Damage from Two Leaffooted Bugs, Leptoglossus clypealis Heidemann and Leptoglossus zonatus (Dallas) (Hemiptera: Coreidae), on Four Almond Varieties

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    Leaffooted bugs (Leptoglossus spp; Hemiptera: Coreidae) are phytophagous insects native to the Western Hemisphere. In California, Leptoglossus clypealis and Leptoglossus zonatus are occasional pests on almonds. Early season feeding by L. clypealis and L. zonatus leads to almond drop, while late season feeding results in strikes on kernels, kernel necrosis, and shriveled kernels. A field cage study was conducted to assess feeding damage associated with L. clypealis and L. zonatus on four almond varieties, Nonpareil, Fritz, Monterey, and Carmel. The objectives were to determine whether leaffooted bugs caused significant almond drop, to pinpoint when the almond was vulnerable, and to determine the final damage at harvest. Branches with ~20 almonds were caged and used to compare almond drop and final damage in four treatments: (1) control branches, (2) mechanically punctured almonds, (3) adult Leptoglossus clypealis, and (4) adult Leptoglossus zonatus. Replicates were set up for eight weeks during two seasons. Early season feeding resulted in higher almond drop than late season, and L. zonatus resulted in greater drop than L. clypealis. The almond hull width of the four varieties in the study did not influence susceptibility to feeding damage. The final damage assessment at harvest found significant levels of kernel strikes, kernel necrosis, and shriveled almonds in bug feeding cages, with higher levels attributed to L. zonatus than L. clypealis. Further research is warranted to develop an Integrated Pest Management program with reduced risk controls for L. zonatus

    Experimental infection of pigs with group A rotavirus and enterotoxigenic Escherichia coli in India: gross, histopathological and immunopathological study

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    The authors describe a detailed study conducted in Assam, India, of gross, histopathological and immunopathological alterations in pigs experimentally infected with rotavirus and enterotoxigenic Escherichia coli (ETEC) expressing K88 pili. A total of 30 Caesarean derived piglets were infected experimentally with rotavirus alone or in combination with ETEC to study the gross and histopathological alterations and the distribution pattern of different B- and T‑cell subsets in the gut. Villus atrophy, especially in the jejunum and ileum, was the consistent lesion in piglets infected with rotavirus, while in piglets simultaneously infected with rotavirus and ETEC, severe necrosis of the intestinal villi was observed. Ultrastructural studies revealed similar pathological alterations in the ileum of the infected piglets. A morphometric study of the intestinal villi and crypts showed a reduction in the ratio between the average villus height and crypt depth (VH:CD ratio) in the group infected with rotavirus (5.95 ± 0.33) and those infected with rotavirus and ETEC (7.90 ± 0.16). A higher (p<0.01) reduction in the VH:CD ratio was observed in the jejunum (8.83 ± 0.79) and ileum (8.46 ± 0.78) compared that in the duodenum (10.03 ± 0.50) of the infected pigs. Piglets infected with rotavirus and sacrificed on day 6 post infection revealed the presence of lymphocytes containing cytoplasmic IgA+ (cIgA+) cells in the villus lamina propria and intra-epithelial CD8+ T‑cells in the villus epithelia. Rotavirus infection of young piglets in association with ETEC was more severe than rotavirus infection alone. Such infection resulted in marked clinico-pathological and immunological alterations in the infected piglets

    Copper Complexes Relevant to the Catalytic Cycle of Copper Nitrite Reductase: Electrochemical Detection of NO(<i>g</i>) Evolution and Flipping of NO<sub>2</sub> Binding Mode upon Cu<sup>II</sup> → Cu<sup>I</sup> Reduction

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    Copper complexes of the deprotonated tridentate ligand, <i>N</i>-2-methylthiophenyl-2′-pyridinecarboxamide (HL1), were synthesized and characterized as part of our investigation into the reduction of copper­(II) <i>o</i>-nitrito complexes into the related copper nitric oxide complexes and subsequent evolution of NO­(<i>g</i>) such as occurs in the enzyme copper nitrite reductase. Our studies afforded the complexes [(L1)­Cu<sup>II</sup>Cl]<sub><i>n</i></sub> (<b>1</b>), [(L1)­Cu<sup>II</sup>(ONO)] (<b>2</b>), [(L1)­Cu<sup>II</sup>(H<sub>2</sub>O)]­(ClO<sub>4</sub>)·H<sub>2</sub>O (<b>3</b>·<b>H</b><sub><b>2</b></sub><b>O</b>), [(L1)­Cu<sup>II</sup>(CH<sub>3</sub>OH)]­(ClO<sub>4</sub>) (<b>4</b>), [(L1)­Cu<sup>II</sup>(CH<sub>3</sub>CO<sub>2</sub>)]·H<sub>2</sub>O (<b>5</b>·<b>H</b><sub><b>2</b></sub><b>O</b>), and [Co­(Cp)<sub>2</sub>]­[(L1)­Cu<sup>I</sup>(NO<sub>2</sub>)­(CH<sub>3</sub>CN)] (<b>6</b>). X-ray crystal structure determinations revealed distorted square-pyramidal coordination geometry around Cu<sup>II</sup> ion in <b>1</b>–<b>5</b>. Substitution of the H<sub>2</sub>O of <b>3</b> by nitrite quantitatively forms <b>2</b>, featuring the κ<sup>2</sup>-O,O binding mode of NO<sub>2</sub><sup>–</sup> to Cu<sup>II</sup>. Reduction of <b>2</b> generates two Cu<sup>I</sup> species, one with κ<sup>1</sup>-O and other with the κ<sup>1</sup>-N bonded NO<sub>2</sub><sup>–</sup> group. The Cu<sup>I</sup> analogue of <b>2</b>, compound <b>6</b>, was synthesized. The FTIR spectrum of <b>6</b> reveals the presence of κ<sup>1</sup>-N bonded NO<sub>2</sub><sup>–</sup>. Constant potential electrolysis corresponding to Cu<sup>II</sup> → Cu<sup>I</sup> reduction of a CH<sub>3</sub>CN solution of <b>2</b> followed by reaction with acids, CH<sub>3</sub>CO<sub>2</sub>H or HClO<sub>4</sub> generates <b>5</b> or <b>3</b>, and NO­(<i>g</i>), identified electrochemically. The isolated Cu<sup>I</sup> complex <b>6</b> independently evolves one equivalent of NO­(<i>g</i>) upon reaction with acids. Production of NO­(g) was confirmed by forming [Co­(TPP)­NO] in CH<sub>2</sub>Cl<sub>2</sub> (λ<sub>max</sub> in CH<sub>2</sub>Cl<sub>2</sub>: 414 and 536 nm, ν<sub>NO</sub> = 1693 cm<sup>–1</sup>)
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