7 research outputs found
1992 Blueberry Research Progress Reports
The 1992 Blueberry Research Progress Reports pertain to and report on research conducted in 1991, and were prepared for the Maine Wild Blueberry Commission and the University of Maine Wild Blueberry Advisory Committee by researchers at the University of Maine, Orono. Projects in this report include:
1992 CSRS Progress Reports:
1. Investigation of Groundwater Resources
2. Sprinkler Irrigation
3. Investigation of Preprocess Changes Leading to Berry Spoilage
4. Effect of Fertilization and Irrigation on Blueberry Quality
5. Effects of Calcium Salts and Citric Acid on Quality of Canned Lowbush Blueberries
6. Pollination of Lowbush Blueberry by Native Bees
7. Application of Heat for Controlling Insects
8. Investigations of Lowbush Blueberry Fruit Bud Cold-Hardiness
9. Steam Sterilization in Lowbush Blueberry Fields
10. Heat-Tolerant Molds
11. Vacuum Sanitation for Disease Control
12. Evaluation of Infrared Burner for Weed Control
13. Evaluation and Modification of Commercial Herbicide Wipers
14. Evaluation of Remote Sensing to Estimate Plant Cover in Lowbush Blueberry Fields
15. Comparison of Three Mechanical Blueberry Harvesters vs. Hand Raking
Advisory Committee Research Reports:
16. Biology and action thresholds of secondary blueberry insects
17. Control of secondary blueberry pests
18. Control of blueberry maggot
19. Effects of calcium salts and citric acid on the quality of canned lowbush blueberries
20. The effects of postharvest handling on the dietary fiber and ellagic acid content of lowbush blueberries
21. Investigation of preprocessing changes that could lead to development of simple and inexpensive method to measure preprocessing berry spoilage
22. Determination of pesticide residue levels in fresh and processed lowbush blueberries
23. Vacuum sanitation for disease control
24. Heat-tolerant molds
25. Seedling pruning study
26. Effect of time and rate of application of Clopyralid for control of Vetch in lowbush blueberries
27. Evaluation and modification of commercial herbicide wipers
28. Effect of time of application and formulation of Hexazinone (Velpar) on Blueberry and Bunchberry
29. Evaluation of postemergence applications of Tribenuron Methyl for Bunchberry control
30. Thresholds of Dogbane and Bracken Fern by mechanical and chemical control in lowbush blueberry fields
31. Evaluation of the suitability of remote sensing to evaluate plant cover in lowbush blueberry fields
32. Evalution of infrared burner for weed control
33. Effect of time of fall pruning on growth and productivity of blueberry and evaluation of infrared burner to prune blueberries
34. Effect of Boron on lowbush blueberry fruit set and yield
35. Winter injury protection by potassium
36. Multiple cropping of wild stands
37. Nitrogen-Phosphorus study
38. Phosphorus dose/response curve
39. Investigations of lowbush blueberry fruit bud cold-hardines
Modeling and mitigation of high-concentration antibody viscosity through structure-based computer-aided protein design.
For an antibody to be a successful therapeutic many competing factors require optimization, including binding affinity, biophysical characteristics, and immunogenicity risk. Additional constraints may arise from the need to formulate antibodies at high concentrations (>150 mg/ml) to enable subcutaneous dosing with reasonable volume (ideally <1.0 mL). Unfortunately, antibodies at high concentrations may exhibit high viscosities that place impractical constraints (such as multiple injections or large needle diameters) on delivery and impede efficient manufacturing. Here we describe the optimization of an anti-PDGF-BB antibody to reduce viscosity, enabling an increase in the formulated concentration from 80 mg/ml to greater than 160 mg/ml, while maintaining the binding affinity. We performed two rounds of structure guided rational design to optimize the surface electrostatic properties. Analysis of this set demonstrated that a net-positive charge change, and disruption of negative charge patches were associated with decreased viscosity, but the effect was greatly dependent on the local surface environment. Our work here provides a comprehensive study exploring a wide sampling of charge-changes in the Fv and CDR regions along with targeting multiple negative charge patches. In total, we generated viscosity measurements for 40 unique antibody variants with full sequence information which provides a significantly larger and more complete dataset than has previously been reported