Determinação de fénois e flavonoides totais dos galhos de Jatropha Mollissima (POHL) Baill. (Pinhão-Bravo)/Determination of total phoenixes and flavonoids of Jatropha Mollissima (POHL) Baill branches. (Pinhão-Bravo)

Jatropha mollissima (Pohl.) Baill., popularmente conhecida como pinhão-bravo, ou pinhão-de-purga, é um pequeno arbusto da família Euphorbiaceae, característico da região da Caatinga, sendo considerado uma das mais ricas fontes de fitoquímicos. Na busca por fitoquímicos com potencial farmacológico, este trabalho tem por objetivo determinar o quantitativo de fenóis e flavonoides totais do extrato etanólico dos galhos de J. mollissima, utilizando os métodos de Folin Ciocalteu e do cloreto de alumínio. Após obtenção das curvas padrões de acido gálico e quercetina, foi possível determinar a concentração de fenóis totais de 711,13 mg EAG/g de extrato seco e  flavonoides totais igual a 19,50 mg de EQuer/g de extrato, evidenciando um resultado promissor e destacando a necessidade de estudos que caracterizem tais compostos

Adverse drug reactions in high-risk pregnant women: A prospective study

Background: Because pregnant women are often excluded from clinical trials, there is still very limited information about the risk and safety of prescription drugs during pregnancy. Objective: We aimed to determine the prevalence of Adverse Drug Reactions (ADRs) in high-risk pregnant women after hospital admission. A prospective study was carried out in a teaching maternity hospital in Brazil during six months. Causality of ADRs was assessed through the Naranjo Algorithm and Korean Algorithm for ADR Causality Assessment. Severity of ADRs was assessed using Hartwig’s Severity Assessment Scale. Results: The prevalence of ADRs among the 294 inpatients studied was 8.8%. The mean age was 27.14 (±7.5) y.o. Patient's age was related to the presence of ADRs, while the manifestation of these events was not associated with any adverse pregnancy outcome. 75.9% of the ADRs reported in the study were of mild severity and 24.1% were of moderate severity. No ADR was caused by drug-drug interaction; however, a significant increase in blood pressure was observed in all patients using concurrent methyldopa and ferrous sulfate. Conclusion: Overall, ADRs were not common events among high-risk pregnant women and no adverse pregnancy outcomes following these events were observed

Analysis toxicity by different methods and anxiolytic effect of the aqueous extract Lippia sidoides Cham.

Abstract Lippia sidoides Cham. (Verbenaceae) is a species often mentioned in traditional medicine due to the medicinal properties attributed to its leaves, which include antibacterial, antifungal, acaricidal and antioxidant. Several of these actions have been scientifically proven, according to reports in the literature; however, little is known about toxicological aspects of this plant. This work included studies to determine the chemical composition and toxicity tests, using several methods aiming to evaluate the safety for use of the aqueous extract of L. sidoides leaves, in addition, the anxiolytic effect on adult zebrafish was investigated, thus contributing to the pharmacological knowledge and traditional medicine concerning the specie under study. The chemical profile was determined by liquid chromatography coupled to mass spectrometry-HPLC/MS with electrospray ionization. Toxicity was evaluated by zebrafish, Drosophila melanogaster, blood cells, and Artemia salina models. 12 compounds belonging to the flavonoid class were identified. In the toxicity assays, the observed results showed low toxicity of the aqueous extract in all tests performed. In the analysis with zebrafish, the highest doses of the extract were anxiolytic, neuromodulating the GABAa receptor. The obtained results support the safe use of the aqueous extract of L. sidoides leaves for the development of new drugs and for the use by populations in traditional medicine