Biochemicka diagnostika poruch vnitrniho prostredi a monitorovani lecby u nemocnych v tezkych stavech.

Available from STL, Prague, CZ / NTK - National Technical LibrarySIGLECZCzech Republi

International federation of clinical chemistry : A strategy to promote the rational use of laboratory tests

Peter Pannall, William Marshall, Antonin Jabor and Erik Magi

Circulating beta‐hydroxybutyrate levels in advanced heart failure with reduced ejection fraction: Determinants and prognostic impact

International audienceAbstract Aims Patients with heart failure (HF) display metabolic alterations, including heightened ketogenesis, resulting in increased beta‐hydroxybutyrate (β‐OHB) formation. We aimed to investigate the determinants and prognostic impact of circulating β‐OHB levels in patients with advanced HF and reduced ejection fraction (HFrEF). Methods and results A total of 867 patients with advanced HFrEF (age 57 ± 11 years, 83% male, 45% diabetic, 60% New York Heart Association class III), underwent clinical and echocardiographic examination, circulating metabolite assessment, and right heart catheterization ( n = 383). The median β‐OHB level was 64 (interquartile range [IQR] 33–161) μmol/L (normal 0–74 μmol/L). β‐OHB levels correlated with increased markers of lipolysis (free fatty acids [FFA]), higher natriuretic peptides, worse pulmonary haemodynamics, and lower humoral regulators of ketogenesis (insulin/glucagon ratio). During a median follow‐up of 1126 (IQR 410–1781) days, there were 512 composite events, including 324 deaths, 81 left ventricular assist device implantations and 107 urgent cardiac transplantations. In univariable Cox regression, increased β‐OHB levels (T3 vs. T1: hazard ratio [HR] 1.39, 95% confidence interval [CI] 1.13–1.72, p = 0.002) and elevated FFA levels (T3 vs. T1: HR 1.39, 95% CI 1.09–1.79, p = 0.008) were both predictors of a worse prognosis. In multivariable Cox analysis evaluating the simultaneous associations of FFA and β‐OHB levels with outcomes, only FFA levels remained significantly associated with adverse outcomes. Conclusions In patients with advanced HFrEF, increased plasma β‐OHB correlate with FFA levels, worse right ventricular function, greater neurohormonal activation and other markers of HF severity. The association between plasma β‐OHB and adverse outcomes is eliminated after accounting for FFA levels, suggesting that increased β‐OHB is a consequence reflecting heightened lipolytic state, rather than a cause of worsening HF

Accuracy and Feasibility of Real-time Continuous Glucose Monitoring in Critically Ill Patients After Abdominal Surgery and Solid Organ Transplantation.

OBJECTIVE: Glycemia management in critical care is posing a challenge in frequent measuring and adequate insulin dose adjustment. In recent years, continuous glucose measurement has gained accuracy and reliability in outpatient and inpatient settings. The aim of this study was to assess the feasibility and accuracy of real-time continuous glucose monitoring (CGM) in ICU patients after major abdominal surgery. RESEARCH DESIGN AND METHODS: We included patients undergoing pancreatic surgery and solid organ transplantation (liver, pancreas, islets of Langerhans, kidney) requiring an ICU stay after surgery. We used a Dexcom G6 sensor, placed in the infraclavicular region, for real-time CGM. Arterial blood glucose measured by the amperometric principle (ABL 800; Radiometer, Copenhagen, Denmark) served as a reference value and for calibration. Blood glucose was also routinely monitored by a StatStrip bedside glucose meter. Sensor accuracy was assessed by mean absolute relative difference (MARD), bias, modified Bland-Altman plot, and surveillance error grid for paired samples of glucose values from CGM and acid-base analyzer (ABL). RESULTS: We analyzed data from 61 patients and obtained 1,546 paired glucose values from CGM and ABL. Active sensor use was 95.1%. MARD was 9.4%, relative bias was 1.4%, and 92.8% of values fell in zone A, 6.1% fell in zone B, and 1.2% fell in zone C of the surveillance error grid. Median time in range was 78%, with minimum (<1%) time spent in hypoglycemia. StatStrip glucose meter MARD compared with ABL was 5.8%. CONCLUSIONS: Our study shows clinically applicable accuracy and reliability of Dexcom G6 CGM in postoperative ICU patients and a feasible alternative sensor placement site

Accuracy and Feasibility of Real-time Continuous Glucose Monitoring in Critically Ill Patients after Abdominal Surgery and Solid Organ Transplantation

Objectives: Glycemia management in critical care is posing a challenge in frequent measuring and adequate insulin dose adjustment. In recent years continuous glucose measurement is gaining accuracy and reliability in outpatient and inpatient setting. The aim of this study was to assess the feasibility and accuracy of real-time continuous glucose monitoring in ICU patients after major abdominal surgery. Research design and methods: We included patients undergoing pancreatic surgery and solid organ transplantation (liver, pancreas, islets of Langerhans, kidney) requiring ICU stay after surgery. We used a Dexcom G6 sensor, placed in the infraclavicular region, for rtCGM. Arterial blood glucose measured by the amperometric principle (ABL 800, Radiometer, Copenhagen, Denmark) served as reference values and for calibration. Blood glucose was also routinely monitored by StatStrip bedside glucose meter. Sensor accuracy was assessed by mean absolute relative difference (MARD), bias, modified Bland-Altman plot and Surveillance Error Grid for paired samples of glucose values from CGM and ABL. Results: We analyzed data from 61 patients and obtained 1546 paired glucose values from CGM and ABL. Active sensor use was 95.1%. MARD was 9.4%, relative bias 1,4%, 92.8% values fell in zone A, 6.1% in zone B and 1.2% in zone C of Surveillance Error Grid. Median time in range was 78%, with minimum (Conclusions: Our study shows clinically applicable accuracy and reliability of Dexcom G6 CGM in postoperative ICU patients and a feasible alternative sensor placement site.</p