Alterações morfológicas em ratas induzidas pelo tratamento prolongado com o bromopride

There were morphoIogicaIy examined the ovaries, uterus, fallopian tubes and mammary glands of adult female rats, that were long-term treated with bromopride. Large corpus luteum, uterus in a secretory stage and hipertrophiated mammary gland were observed in treated animals. These marked morphological changes were correlated to the neuroendocrinal effects of the drug, considering what it is able to block tuberoinfundibuIar dopamine (DA) receptors leading to an increase in prolactin levels.Foram examinados ovários, úteros, trompas de falópio e glândulas mamárias, provenientes de ratas tratadas prolongadamente com o bromopride. Foram observados corpos lúteos muito desenvolvidos, úteros em fase secretória e glândulas mamárias hipertrofiadas. Estas alterações morfológicas marcantes estão correlacionadas com os- efeitos neuroendócrinos da droga, pois esta é capaz de bloquear os dopaninoceptores do sistema tuberoinfundibular, aumentando, assim, os níveis séricos de prolactin

Factores nutricionales y los niveles de hemoglobina en gestantes del Centro de Salud Chaglla Huanuco, de enero a junio del 2021

El objetivo de nuestra investigación fue determinar la relación entre los factores nutricionales y los niveles de hemoglobina en gestantes del Centro de Salud Chaglla Huánuco, enero a junio del 2021. Metodología, se realizó un estudio de tipo básico, transversal, retroprospectivo, con diseño correlacional. Se trabajó con una población muestral de 130 gestantes que acudieron al Centro de Salud entre los periodos de enero a junio del 2021. Se utilizó como técnicas: el análisis documental y la encuesta; como instrumentos, la ficha de evaluación del estado nutricional pregestacional y del nivel de hemoglobina así también el cuestionario de hábitos alimenticios y consumo de hierro. Los instrumentos fueron validados por juicio de expertos y la confiabilidad a través del Alfa de Cronbach (0,780). Para constatar las hipótesis se utilizó la prueba estadística Tau b de Kendall (t) para relacionar las variables ordinales del estudio. Resultados: los resultados determinaron relación moderada, positiva, y significativa (t=0,451 p=0,000) entre los factores nutricionales medianamente adecuados (46,9%) y el nivel de hemoglobina normal (89,2%). En sus dimensiones, se comprobó relación baja, positiva y significativa (t=0,245 p=0,002) entre el estado nutricional pregestacional normal (44,6%) y el nivel de hemoglobina normal (89,2%). No se evidenció relación entre el consumo de hierro y el nivel de hemoglobina (t=0,368 p=0,000). Conclusión: se concluyó que la relación entre los factores nutricionales y el nivel de hemoglobina es moderada y significativa

Analysis of shared common genetic risk between amyotrophic lateral sclerosis and epilepsy

Because hyper-excitability has been shown to be a shared pathophysiological mechanism, we used the latest and largest genome-wide studies in amyotrophic lateral sclerosis (n = 36,052) and epilepsy (n = 38,349) to determine genetic overlap between these conditions. First, we showed no significant genetic correlation, also when binned on minor allele frequency. Second, we confirmed the absence of polygenic overlap using genomic risk score analysis. Finally, we did not identify pleiotropic variants in meta-analyses of the 2 diseases. Our findings indicate that amyotrophic lateral sclerosis and epilepsy do not share common genetic risk, showing that hyper-excitability in both disorders has distinct origins

In vitro macrophage activity: Biphasic effect of prolactin and indirect evidence of dopaminergic modulation

Objective: Prolactin (PRL), a peptide hormone produced by the pituitary gland, is involved in the interaction between the neuroendocrine and immune system. Since dopamine receptor antagonists increase serum levels of PRL, both PRL and dopamine receptors might be involved in the modulation of macrophage activity, providing means of communication between the nervous and immune systems. This study evaluated the effects of PRL and the dopamine antagonist domperidone (DOMP) on macrophage activity of female rats. Methods: Oxidative burst and phagocytosis of peritoneal macrophages were evaluated by flow cytometry. Samples of peritoneal liquid from female rats were first incubated with PRL (10 and 100 nM) for different periods. The same procedure was repeated to evaluate the effects of DOMP (10 and 100 nM). Results: In vitro incubation of macrophages with 10 nM DOMP decreased oxidative burst, after 30 min, whereas the PMA-induced burst was decreased by DOMP 10 nM after 2 and 4 h. Treatment with PRL (10 and 100 nM) for 30 min decreased oxidative burst and rate of phagocytosis (10 nM). After 2 h of incubation, 10 nM PRL decreased oxidative burst and phagocytosis intensity, but increased the rate of phagocytosis. On the other hand, after 4 h, PRL 10 and 100 nM increased oxidative burst and the rate of phagocytosis, but decreased intensity of phagocytosis. Conclusions: These observations suggest that macrophage functions are regulated by an endogenous dopaminergic tone. Our data also suggest that both PRL and dopamine exert their action by acting directly on the peritoneal macrophage. Copyright (C) 2008 S. Karger AG, Basel.Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)[05/56833-4]Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)[04/14128-0]Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES

Additional file 2: of Dintor: functional annotation of genomic and proteomic data

Data files for Dintor use-cases one to three

Additional file 1: of Dintor: functional annotation of genomic and proteomic data

Dintor tools summary. (PDF 567 kb