El Proyecto Alzheimer MuBAM. Accesibilidad a través del Arte

SIAM. Series Iberoamericanas de Museología. Año 3, Vol.

Multiomics integrative analysis identifies APOE allele-specific blood biomarkers associated to Alzheimer's disease etiopathogenesis

Alzheimer’s disease (AD) is the most common form of dementia, currently affecting 35 million people worldwide. Apolipoprotein E (APOE) ε4 allele is the major risk factor for sporadic, late-onset AD (LOAD), which comprises over 95% of AD cases, increasing the risk of AD 4-12 fold. Despite this, the role of APOE in AD pathogenesis is still a mystery. Aiming for a better understanding of APOE-specific effects, the ADAPTED consortium analysed and integrated publicly available data of multiple OMICS technologies from both plasma and brain stratified by APOE haplotype (APOE2, APOE3 and APOE4). Combining genome-wide association studies (GWAS) with differential mRNA and protein expression analyses and single-nuclei transcriptomics, we identified genes and pathways contributing to AD in both APOE dependent and independent fashion. Interestingly, we characterised a set of biomarkers showing plasma and brain consistent protein profiles and opposite trends in APOE2 and APOE4 AD cases that could constitute screening tools for a disease that lacks specific blood biomarkers. Beside the identification of APOE-specific signatures, our findings advocate that this novel approach, based on the concordance across OMIC layers and tissues, is an effective strategy for overcoming the limitations of often underpowered single-OMICS studies

A Critical Mutualism - Competition Interplay Underlies the Loss of Microbial Diversity in Sedentary Lifestyle

Physical exercise improves the overall health status by preventing the development of several diseases. In recent years, it has been observed that physical exercise impacts gut microbiota by increasing the presence of beneficial bacteria and microbial diversity. In contrast, a sedentary lifestyle increases the incidence of chronic diseases that often have an associated loss of microbial diversity. The gut microbiota is a vast ecosystem in which microorganisms interact with each other in different ways; however, microbial ecosystem interactions are scarcely studied. The goal of this study was to determine whether individuals with a sedentary lifestyle have lower diversity in their gut microbiota and how microbial diversity is associated with changes in bacterial network interactions. For that purpose, diet, body composition, physical activity, and sedentarism behavior were characterized for individuals who did or did not comply with the World Health Organization recommendations for physical activity. The composition of the gut microbiome was determined by 16S rRNA gene sequencing. Reorganization of microbial structure with lifestyle was approached from network analysis, where network complexity and the topology of positive and negative interdependences between bacteria were compared and correlated with microbial diversity. Sedentary lifestyle was significantly associated with a diet low in fiber and rich in sugars and processed meat, as well as with high visceral and total corporal fat composition. The diversity (phylogenic diversity, Chao, observed species, and Shannon's index) and network complexity of the gut microbiota were significantly lower in sedentary compared to active individuals. Whereas mutualism or co-occurrence interactions were similar between groups, competitiveness was significantly higher in the active lifestyle group. The mutualism-competition ratio was moderate and positively associated with diversity in sedentary individuals, but not in active individuals. This finding indicates that there is a critical point in this ratio beyond which the stability of the microbial community is lost, inducing a loss of diversity.Ministerio de Economía y Competitividad. Ayudas Ramón y Cajal (2012-11910)MINECO (AGL2016-75878-R)UEM17155.640 JCR (2020) Q1, 28/136 Microbiology1.701 SJR (2020) Q1, 30/152 MicrobiologyNo data IDR 2020UE

Key Bacteria in the Gut Microbiota Network for the Transition between Sedentary and Active Lifestyle

Physical activity modifies the gut microbiota, exerting health benefits on the host; however, the specific bacteria associated with exercise are not yet known. In this work, we propose a novel method, based on hierarchical topology, to study the differences between the microbiota of active and sedentary lifestyles, and to identify relevant bacterial taxa. Our results show that the microbiota network found in active people has a significantly higher overall efficiency and higher transmissibility rate. We also identified key bacteria in active and sedentary networks that could be involved in the conversion of an active microbial network to a sedentary microbial network and vice versa.Ramón y Cajal program (grant 2012_11910)MINECO (Project AGL2016-75878-R)Mindfulness and Cognitive Chair of the Universidad Complutense de Madrid, Spain4.128 JCR (2020) Q2, 52/136 Microbiology0.858 SJR (2020) Q2, 75/152 MicrobiologyNo data IDR 2020UE

Unravelling the gut microbiota interactions in sedentary lifestyle

Sin financiaciónNo data 2018UE

Grupo de ayuda mutua para familiares de pacientes con demencia. “Cuidado al cuidador”

From the Dementia Unit at the Hospital Virgen de la Arrixaca, we consider the need to implement a therapeutic group for relatives of patients diagnosed with dementia, addressed “to the care the carer”. Ten sessions were conducted with caregivers, all women, aged between 30 and 50, in which they explained the concept of “Caregiver’s Syndrome”, the guidelines to be considered for the management of behavioral and psychological symptoms own dementia, group dynamics were made to favor the expression of emotions, as well as develop social skills training. Each group session ended with relaxation training and practice in imagination with induction of self-esteem. There was a decrease in the scale of the Zarit caregiver burden, and the Beck Depression Inventory (BDI), administered before and after the completion of the program. Group members said they had a very satisfying experience with the group through a semistructured interview were administered and in the sharing that took place in the last session.Desde la Unidad de Demencias del Hospital Virgen de la Arrixaca, consideramos la necesidad de poner en marcha un grupo terapéutico para los familiares de los pacientes diagnosticados de demencias, dirigido “al cuidado al cuidador”. Se realizaron diez sesiones con cuidadores, todas mujeres, de una edad comprendida entre 30 y 50 años, en las que se les explicaron el concepto de “síndrome del cuidador”, las pautas a tener en cuenta para el manejo de los síntomas psicológicos y conductuales propios de la demencia, se realizaron dinámicas de grupo para favorecer la expresión de emociones, así como desarrollar un entrenamiento en habilidades sociales. Cada sesión grupal finalizaba con ejercicios de relajación y práctica en imaginación con inducción de la autoestima. Se observó una disminución en la Escala de Sobrecarga del Cuidador de Zarit, y el Inventario de Depresión de Beck (BDI), administradas antes y después de la realización del programa. Los miembros del grupo afirmaron haber tenido una experiencia muy satisfactoria con el grupo mediante una entrevista semiestructurada que se les aplicó así como en la puesta en común que tuvo lugar en la última sesión

Clinical manifestations of intermediate allele carriers in Huntington disease

Objective: There is controversy about the clinical consequences of intermediate alleles (IAs) in Huntington disease (HD). The main objective of this study was to establish the clinical manifestations of IA carriers for a prospective, international, European HD registry. Methods: We assessed a cohort of participants at risk with <36 CAG repeats of the huntingtin (HTT) gene. Outcome measures were the Unified Huntington's Disease Rating Scale (UHDRS) motor, cognitive, and behavior domains, Total Functional Capacity (TFC), and quality of life (Short Form-36 [SF-36]). This cohort was subdivided into IA carriers (27-35 CAG) and controls (<27 CAG) and younger vs older participants. IA carriers and controls were compared for sociodemographic, environmental, and outcome measures. We used regression analysis to estimate the association of age and CAG repeats on the UHDRS scores. Results: Of 12,190 participants, 657 (5.38%) with <36 CAG repeats were identified: 76 IA carriers (11.56%) and 581 controls (88.44%). After correcting for multiple comparisons, at baseline, we found no significant differences between IA carriers and controls for total UHDRS motor, SF-36, behavioral, cognitive, or TFC scores. However, older participants with IAs had higher chorea scores compared to controls (p 0.001). Linear regression analysis showed that aging was the most contributing factor to increased UHDRS motor scores (p 0.002). On the other hand, 1-year follow-up data analysis showed IA carriers had greater cognitive decline compared to controls (p 0.002). Conclusions: Although aging worsened the UHDRS scores independently of the genetic status, IAs might confer a late-onset abnormal motor and cognitive phenotype. These results might have important implications for genetic counseling. ClinicalTrials.gov identifier: NCT01590589